scholarly journals P081 The structure of the proteomic profile and gut microbiota in patients with primary immunodeficiency, common variable immunodeficiency and inflammatory bowel disease

2020 ◽  
Vol 14 (Supplement_1) ◽  
pp. S175-S176
Author(s):  
A A Iakovlev ◽  
A Volkov ◽  
O Bashtovaya ◽  
I Shcherbakova ◽  
T Ghazudin ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Primary Immunodeficiency ◽  
Common Variable Immunodeficiency ◽  
Fold Increase ◽  
Control Group ◽  
Microbiological Analysis ◽  
Colon Mucosa ◽  
Hydrogen Breath Test ◽  
E Coli ◽  
Common Variable

Abstract Background Infringement of intestinal microbiota in patients with primary immunodeficiency (PID), common variable immunodeficiency (CVID) and ulcerative colitis (UC) continues to be studied. The aim of the research is a comprehensive study of the structure of gut microbiota and mucosal protein profile (MPP) in patients with PID, CVID and UC in different phases of the disease course. Methods The research included 15 patients with PID, 22 CVID and 46 UC (28 with relapse and 18 with persistent remission of the disease), control group—20 healthy volunteers. Estimation of gut microbiota was performed by bacteriological seeding, hydrogen breath test (HBT) with lactulose. Content of short-chain fatty acids (SCFAs) and microbial lipid markers (MLM) in faeces and colon mucosa was determined by gas–liquid chromatographic and gas chromatography–mass spectrometry (GC–MS). MPP was based on isoelectric focusing techniques (SDS–PAGE, 2DGE). Mass spectrograms were obtained using MALDI-TOF-MS/MS (Bruker, USA). Results HBT showed a 4.5-, 8- and 11-fold increase in hydrogen production on a 150th-minute study. SCFA showed a 6-, 9- and 11-fold decrease in propionic and butyric acids, mainly in patients with PID and UC: 0.2 + 0.1, 0.14 + 0.03 and 0.04 + 0.02 mg/g, respectively. Microbiological analysis showed a decrease in titers of E. coli, bifido- and lactobacilli on average 4.6 + 0.8 Lg. The conditionally pathogenic microflora was represented by E. coli, lactose-negative strains (n = 51,105/g) and haemolytic strains (n = 34,104/g, Proteus spp. (n = 48,105/g), Staphylococcus spp. (n = 29,105–6/g), Candida (n = 51,104–6/g) and Clostridium spp. (n = 38,104/g) in stool culture. MLN GS-MS results showed a 5.5-, 3- and 6-fold increase in total bacterial load in patients with PID, CVID and UC, respectively, which was represented by resident anaerobic microflora: Streptococcus mutants, Clostridium difficile, Candida albicans. Results of MPP (detection rate of 75% or more) of the colon mucosa in patients with PID were detected: 1, 2, 4 okkludin, kininogen 1, interleukin-1B, interleukin 8, B2-glycoprotein, heat shock protein 27, in patients with CVID—translational elongation factor, apolipoprotein C-III. In patients with UC—NF-kB, alipoprotein C-III, TNF-α, interleukin-2 and 8 were presented. Conclusion In patients with PID, CVID and UC markers of colon excess growth and significant decrease in SCFA are recorded during the relapse period. MPP in patients with PID, CVID and UC was characterised by proteins characteristic of inflammation, apoptosis, proliferation and proteins reflecting the activity of the autoimmune process. Patients with UC in the remission have recovery trend of gut microbiota, production of SCFA, disappearance of specific components of altered MPP.

scholarly journals Assessment of Gut Microbiota and Selected Blood Parameters in Weaned Piglets Following Supplementation with a Probiotic and Vitamin C

2020 ◽  
Vol 20 (1) ◽  
pp. 179-189
Author(s):  
Leszek Tymczyna ◽  
Beata Trawińska ◽  
Marta Kowaleczko ◽  
Anna Chmielowiec-Korzeniowska ◽  
Jerzy Lechowski
Keyword(s):  
Vitamin C ◽  
Gut Microbiota ◽  
Total Cholesterol ◽  
Hdl Cholesterol ◽  
Blood Parameters ◽  
Control Group ◽  
Weaned Piglets ◽  
E Coli ◽  
Experimental Group ◽  
Control Group Group

AbstractThe aim of the study was to assess the gut microbiota and selected haematological and biochemical blood parameters of weaned piglets following dietary supplementation with a probiotic and vitamin C. the piglets were divided into a control group (group C) and an experimental group (group E), with 30 piglets in each group. All animals received the same feed ad libitum. The animals in the control group (group C) received feed with no added probiotic and vitamin C. the piglets in the experimental group (group E) were given a supplement containing a probiotic (Bacillus cereus 1×109 CFU/kg) in the amount of 1.5 g/piglet/day and vitamin c in the amount of 300 mg/piglet/day. The supplement was administered for 28 days. The total numbers of bacteria of the family Enterobacteriaceae and of the genus Lactobacillus were determined in faeces. The erythrocyte count, haemoglobin level, haematocrit, leukocyte count, and percentages of neutrophils and lymphocytes were determined in the blood. The biochemical analysis concerned the concentration of triacylglycerols, total cholesterol, and LDL and HDL cholesterol. In group E a significant decrease (P<0.01) in the total number of Enterobacteriaceae bacteria was observed in the faeces of the piglets, accompanied by an increase in the number of lactobacilli relative to group C. E. coli was found to predominate over other microorganisms. Salmonella choleraesuis bacteria were present in the faeces of both groups before administration of the supplement, but were not found after its use. The supplement with probiotic and vitamin C caused a significant increase in the erythrocyte, haemoglobin and haematocrit levels in the blood of the piglets and a significant decrease in the concentration of triacylglycerols, total cholesterol and LDL cholesterol.

scholarly journals Common variable immunodeficiency – case report

2013 ◽  
Vol 3 (2) ◽  
pp. 170-172
Author(s):  
Emina Vukas ◽  
Aida Dizdarević ◽  
Senka Mesihović - Dinarević ◽  
Adisa Čengić
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Case Report ◽  
Immune System ◽  
Primary Immunodeficiency ◽  
Common Variable Immunodeficiency ◽  
Clinical Picture ◽  
Clinical Signs ◽  
Common Variable

Common variable immunodeficiency (CVID) or acquired hypogammaglobulinemia is the type of primary immunodeficiency. Deregulation of the immune system, leading to hypogammaglobulinemia, defective activation and proliferation of T cells and dendritic cells, and malfunction of the cytokines are observed in CVID. The clinical picture of CVID varies, any organ or system can be affected, therefore the diagnosis is often difficult and delayed and sometimes is not always possible. This article describes a twelve years old boy with all the clinical signs of immunodeficiency, as confi rmed by laboratory. The main treatment consists of life-long immunoglobulin substitution in intravenous or subcutaneous form.

Monogenic Primary Immunodeficiency Disorder Associated with Common Variable Immunodeficiency and Autoimmunity

2020 ◽  
Vol 181 (9) ◽  
pp. 706-714 ◽  
Author(s):  
Mohammad Hossein Asgardoon ◽  
Gholamreza Azizi ◽  
Reza Yazdani ◽  
Mahsa Sohani ◽  
Salar Pashangzadeh ◽  
...  
Keyword(s):  
Primary Immunodeficiency ◽  
Common Variable Immunodeficiency ◽  
Primary Immunodeficiency Disorder ◽  
Immunodeficiency Disorder ◽  
Common Variable

scholarly journals Gut microbiota in Mexican patients with common variable immunodeficiency

2020 ◽  
Vol 155 (5) ◽  
Author(s):  
Ana Paola Franco-Esquivias ◽  
Cristina García-De la Peña ◽  
Carlos Torres-Lozano ◽  
Felipe Vaca-Paniagua ◽  
Clara Díaz-Velásquez ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Common Variable Immunodeficiency ◽  
Common Variable ◽  
Mexican Patients

scholarly journals Salivary Immunoglobulins in Individuals with Common Variable Immunodeficiency

2016 ◽  
Vol 27 (6) ◽  
pp. 641-645 ◽  
Author(s):  
Karin Sá Fernandes ◽  
Michella Bezerra Lima ◽  
Cíntia de Paula Martins ◽  
Maria Cristina dos-Santos ◽  
Fabio Daumas Nunes ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Common Variable Immunodeficiency ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Salivary Iga ◽  
Serum Iga ◽  
Whole Saliva ◽  
Few Data ◽  
Immunoglobulin Levels ◽  
Common Variable

Abstract Oral manifestations of common variable immunodeficiency (CVID) are rare, have rarely been studied and have given controversial results. There are few data about IgA, IgG, and IgM antibody salivary levels in the literature, and there are few papers about the clinical impact of antibody deficiencies and CVID on the oral health of such patients. The aim of this study was to measure serum and salivary IgA, IgG, and IgM levels in CVID participants and controls, and to associate immunoglobulin levels with caries and periodontal disease. This was a case-control study involving 51 CVID individuals and 50 healthy controls. All participants underwent examination for dental caries and periodontal disease. Blood and whole saliva samples were collected on the same day of the oral examination. Serum IgA, IgM, and IgG levels were measured by turbidimetry and salivary IgA, IgM, and IgG titers were assessed by enzyme-linked immunosorbent assay. Incidences of caries and gingivitis were significantly higher in the CVID group than in the control group (p<0.05). Salivary and blood IgA and IgM titers were significantly reduced in the CVID group, but there was no association of salivary immunoglobulin levels with periodontal disease or with caries incidence (p>0.05 for both). Although CVID was associated with increased susceptibility to caries and gingivitis, it was not associated with low salivary levels of IgA and IgM.

scholarly journals Gut Microbiota-Dependent Trimethylamine N-Oxide Associates With Inflammation in Common Variable Immunodeficiency

2020 ◽  
Vol 11 ◽  
Author(s):  
Magnhild E. Macpherson ◽  
Johannes R. Hov ◽  
Thor Ueland ◽  
Tuva B. Dahl ◽  
Martin Kummen ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Common Variable Immunodeficiency ◽  
Common Variable

scholarly journals Common variable immunodeficiency and isosporiasis: first report case

2012 ◽  
Vol 45 (6) ◽  
pp. 768-769 ◽  
Author(s):  
Gisele Barbosa e Silva ◽  
Karla Pereira Fernandes ◽  
Gesmar Rodrigues Silva Segundo
Keyword(s):  
Primary Immunodeficiency ◽  
Common Variable Immunodeficiency ◽  
Giardia Lamblia ◽  
Opportunistic Infections ◽  
Severe Case ◽  
Negative Patient ◽  
Immunological Status ◽  
Laboratory Investigations ◽  
Common Variable ◽  
Hiv Negative

We report a severe case of diarrhea in a 62-year-old female HIV-negative patient from whom Giardia lamblia and Isospora belli were isolated. Because unusual and opportunistic infections should be considered as criteria for further analysis of immunological status, laboratory investigations led to a diagnosis of common variable immunodeficiency (CVID). This is the first reported case of isosporiasis in a patient with CVID and illustrates the importance of being aware of a possible link, particularly in relation to primary immunodeficiency.

scholarly journals A Young Girl with Repeated Episodes of Pneumonia and Intermittednt Diarrhoea - The Primary Immunodeficiency Syndrome

2013 ◽  
Vol 22 (1) ◽  
pp. 24-26
Author(s):  
Abdullah Al-Mamun ◽  
Samim Hasan ◽  
Syed Ahmed Abdullah ◽  
Ahmed Riyad Hossain ◽  
Safiul Alam ◽  
...  
Keyword(s):  
Replacement Therapy ◽  
Primary Immunodeficiency ◽  
Common Variable Immunodeficiency ◽  
Young Girl ◽  
Organ Specific ◽  
Relative Rarity ◽  
Immunoglobulin Replacement ◽  
Immunodeficiency Syndrome ◽  
Work Up ◽  
Common Variable

A description is given of a case of a young girl who presented with repeated episodes of pneumonia since childhood. In addition, she had episodes of intermittent diarrhoea. Her investigative work-up revealed a deficiency of serum immunoglobulins. The profile was suggestive of common variable immunodeficiency. Because of a relative rarity of this disease, it is often misdiagnosed earlier. Late diagnosis and delayed institution of immunoglobulin replacement therapy results in increased morbidity with a wide variety of organ-specific complications and increased mortality. DOI: http://dx.doi.org/10.3329/bjmed.v22i1.13597 Bangladesh J Medicine 2011; 22: 24-26

scholarly journals Features of the cytokine profile in humoral primary immunodeficiency patients

2015 ◽  
Vol 12 (1) ◽  
pp. 34-37
Author(s):  
L P Sizyakina ◽  
I I Andreeva ◽  
E A Antonova
Keyword(s):  
Primary Immunodeficiency ◽  
Common Variable Immunodeficiency ◽  
Serum Levels ◽  
Cytokine Profile ◽  
Common Variable

The results of cytokines serum levels in patients with infectious and autoimmune phenotypes of X-linked agammaglobulinemia (X-AGG) and common variable immunodeficiency (CVID) are presented. In patients with varying nature of the primary agammaglobulinemia similar cytokine changes were estimated.

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