scholarly journals Relationship between chromosome 9 of maize and wheat homeologous group 7 chromosomes.

Genetics ◽  
1994 ◽  
Vol 138 (4) ◽  
pp. 1287-1292
Author(s):  
K M Devos ◽  
S Chao ◽  
Q Y Li ◽  
M C Simonetti ◽  
M D Gale

Abstract Comparison of the genetic map of maize chromosome 9 with maps of wheat chromosomes has revealed a high degree of colinearity between maize chromosome 9 and the group 4 and 7 chromosomes of wheat. The order of DNA markers on the short arm and a proximal region of the long arm of the genetic map of maize chromosome 9 is highly conserved with the marker order on the short arm and proximal region of the long arm of the genetic map of the wheat homeologous group 7 chromosomes. A major part of the long arm of the genetic map of maize chromosome 9 is homeologous with a short segment in the proximal region of the long arm of the genetic map of the wheat group 4 chromosomes. Evidence is also presented that maize chromosome 9 has diverged from the wheat group 7 chromosomes by both a pericentric and a paracentric inversion. The paracentric inversion is probably unique to maize among the major cereal genomes.

Genetics ◽  
2000 ◽  
Vol 156 (1) ◽  
pp. 327-339 ◽  
Author(s):  
O Riera-Lizarazu ◽  
M I Vales ◽  
E V Ananiev ◽  
H W Rines ◽  
R L Phillips

Abstract In maize (Zea mays L., 2n = 2x = 20), map-based cloning and genome organization studies are often complicated because of the complexity of the genome. Maize chromosome addition lines of hexaploid cultivated oat (Avena sativa L., 2n = 6x = 42), where maize chromosomes can be individually manipulated, represent unique materials for maize genome analysis. Maize chromosome addition lines are particularly suitable for the dissection of a single maize chromosome using radiation because cultivated oat is an allohexaploid in which multiple copies of the oat basic genome provide buffering to chromosomal aberrations and other mutations. Irradiation (gamma rays at 30, 40, and 50 krad) of a monosomic maize chromosome 9 addition line produced maize chromosome 9 radiation hybrids (M9RHs)—oat lines possessing different fragments of maize chromosome 9 including intergenomic translocations and modified maize addition chromosomes with internal and terminal deletions. M9RHs with 1 to 10 radiation-induced breaks per chromosome were identified. We estimated that a panel of 100 informative M9RHs (with an average of 3 breaks per chromosome) would allow mapping at the 0.5- to 1.0-Mb level of resolution. Because mapping with maize chromosome addition lines and radiation hybrid derivatives involves assays for the presence or absence of a given marker, monomorphic markers can be quickly and efficiently mapped to a chromosome region. Radiation hybrid derivatives also represent sources of region-specific DNA for cloning of genes or DNA markers.


Genetics ◽  
2004 ◽  
Vol 166 (4) ◽  
pp. 1923-1933 ◽  
Author(s):  
Lorinda K Anderson ◽  
Naser Salameh ◽  
Hank W Bass ◽  
Lisa C Harper ◽  
W Z Cande ◽  
...  

Abstract Genetic linkage maps reveal the order of markers based on the frequency of recombination between markers during meiosis. Because the rate of recombination varies along chromosomes, it has been difficult to relate linkage maps to chromosome structure. Here we use cytological maps of crossing over based on recombination nodules (RNs) to predict the physical position of genetic markers on each of the 10 chromosomes of maize. This is possible because (1) all 10 maize chromosomes can be individually identified from spreads of synaptonemal complexes, (2) each RN corresponds to one crossover, and (3) the frequency of RNs on defined chromosomal segments can be converted to centimorgan values. We tested our predictions for chromosome 9 using seven genetically mapped, single-copy markers that were independently mapped on pachytene chromosomes using in situ hybridization. The correlation between predicted and observed locations was very strong (r2 = 0.996), indicating a virtual 1:1 correspondence. Thus, this new, high-resolution, cytogenetic map enables one to predict the chromosomal location of any genetically mapped marker in maize with a high degree of accuracy. This novel approach can be applied to other organisms as well.


Genetics ◽  
2001 ◽  
Vol 157 (4) ◽  
pp. 1735-1747 ◽  
Author(s):  
Devinder Sandhu ◽  
Julie A Champoux ◽  
Svetlana N Bondareva ◽  
Kulvinder S Gill

AbstractThe short arm of Triticeae homeologous group 1 chromosomes is known to contain many agronomically important genes. The objectives of this study were to physically localize gene-containing regions of the group 1 short arm, enrich these regions with markers, and study the distribution of genes and recombination. We focused on the major gene-rich region (“1S0.8 region”) and identified 75 useful genes along with 93 RFLP markers by comparing 35 different maps of Poaceae species. The RFLP markers were tested by gel blot DNA analysis of wheat group 1 nullisomic-tetrasomic lines, ditelosomic lines, and four single-break deletion lines for chromosome arm 1BS. Seventy-three of the 93 markers mapped to group 1 and detected 91 loci on chromosome 1B. Fifty-one of these markers mapped to two major gene-rich regions physically encompassing 14% of the short arm. Forty-one marker loci mapped to the 1S0.8 region and 10 to 1S0.5 region. Two cDNA markers mapped in the centromeric region and the remaining 24 loci were on the long arm. About 82% of short arm recombination was observed in the 1S0.8 region and 17% in the 1S0.5 region. Less than 1% recombination was observed for the remaining 85% of the physical arm length.


Author(s):  
Svetlana M. Kleyman ◽  
Aruna J. Parekh ◽  
Abraham R. Rodriguez ◽  
Robert A. Conte ◽  
Ram S. Verma

1977 ◽  
Vol 29 (1) ◽  
pp. 83-92 ◽  
Author(s):  
Mary F. Lyon ◽  
P. H. Glenister

SUMMARYThe frequency of adjacent-2 disjunction in mice carrying the reciprocal translocation T(9; 17)138Ca was studied by mating together animals heterozygous for the translocation and carrying different recessive marker genes, using Tt for chromosome 17 and cwcw for chromosome 9. The proportion of marked young arising from adjacent-2 disjunction varied according to the markers carried in the two parents. When the female carried Tt the frequencies of marked young were always higher than when non-T females were used, and when Tt and cwcw were carried in the same parent there was a shortage of marked young obtaining both copies of the proximal region of chromosome 17 from the father. Both these effects were regarded as probably another example of the phenomenon discovered by Johnson, of inviability of young lacking a maternal homologue of a certain region of chromosome 17. There were other variations in frequency of marked young, among crosses using non-T females, which may have been due to differences in transmission ratio of male gametes carrying various t-haplotypes or to true variations in frequency of adjacent-2 disjunction.


2001 ◽  
Vol 102 (5) ◽  
pp. 658-663 ◽  
Author(s):  
L. J. Li ◽  
K. Arumuganathan ◽  
H. W. Rines ◽  
R. L. Phillips ◽  
O. Riera-Lizarazu ◽  
...  

Genome ◽  
2003 ◽  
Vol 46 (4) ◽  
pp. 665-672 ◽  
Author(s):  
E Schnabel ◽  
O Kulikova ◽  
R V Penmetsa ◽  
T Bisseling ◽  
D R Cook ◽  
...  

The sunn mutation of Medicago truncatula is a single-gene mutation that confers a novel supernodulation phenotype in response to inoculation with Sinorhizobium meliloti. We took advantage of the publicly available codominant PCR markers, the high-density genetic map, and a linked cytogenetic map to define the physical and genetic region containing sunn. We determined that sunn is located at the bottom of linkage group 4, where a fine-structure genetic map was used to place the locus within a ~400-kb contig of bacterial artificial chromosome (BAC) clones. Genetic analyses of the sunn contig, as well as of a second, closely linked BAC contig designated NUM1, indicate that the physical to genetic distance within this chromosome region is in the range of 1000 –1100 kb·cM–1. The ratio of genetic to cytogenetic distance determined across the entire region is 0.3 cM·μm–1. These estimates are in good agreement with the empirically determined value of ~300 kb·μm–1 measured for the NUM1 contig. The assignment of sunn to a defined physical interval should provide a basis for sequencing and ultimately cloning the responsible gene.Key words: FISH, physical to genetic distance, Medicago truncatula, map-based cloning.


1999 ◽  
Vol 181 (15) ◽  
pp. 4680-4685 ◽  
Author(s):  
Gregg S. Pettis ◽  
Shubha Prakash

ABSTRACT A database search revealed extensive sequence similarity betweenStreptomyces lividans plasmid pIJ101 andStreptomyces plasmid pSB24.2, which is a deletion derivative of Streptomyces cyanogenus plasmid pSB24.1. The high degree of relatedness between the two plasmids allowed the construction of a genetic map of pSB24.2, consisting of putative transfer and replication loci. Two pSB24.2 loci, namely, thecis-acting locus for transfer (clt) and the transfer-associated korB gene, were shown to be capable of complementing the pIJ101 clt and korBfunctions, respectively, a result that is consistent with the notion that pIJ101 and the parental plasmid pSB24.1 encode highly similar, if not identical, conjugation systems.


Author(s):  
Bojan Leontijević ◽  
Aleksandar Janković ◽  
Lazar Tomić

The aim of this study was to compare the attacking performance profiles of the football teams playing in the national leagues of different rankings, defined on the basis of the Union of European Football Associations (UEFA) coefficient regarding club competitions. Applying the method of random sampling, one national league belonging to the first group – 4 participants in the Champions League and 3 teams participating in the Europa League (CL4+EL3; Germany), one belonging to the second group – CL3+EL3 (France), one belonging to the third group (CL2+EL3; Austria) and one from the fourth group – CL1+EL3 (Serbia) were selected. The analysis included all championship matches within the mentioned national competitions during the 2016/2017 season, which was a total of 1162 matches. The variables related to ball possession, passing game structure and efficacy and goal scoring attacks were monitored (19 variables in total). The results of this study have shown that there are significant differences in the organization of attacking games by the teams competing in the leagues of different rankings. It can be concluded that the players of the teams from the German and French leagues possess higher quality in technical and tactical sense, and are trained to play extremely fast, with a high degree of success in ball control.


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