scholarly journals CBP-1 Acts in GABAergic Neurons to Double Life Span in Axenically Cultured Caenorhabditis elegans

2017 ◽  
Vol 74 (8) ◽  
pp. 1198-1205 ◽  
Author(s):  
Huaihan Cai ◽  
Ineke Dhondt ◽  
Lieselot Vandemeulebroucke ◽  
Caroline Vlaeminck ◽  
Madina Rasulova ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Life Span ◽  
Dietary Restriction ◽  
Gabaergic Neurons ◽  
Creb Binding Protein ◽  
Axenic Medium ◽  
Life Span Extension ◽  
Gene Functions ◽  
Key Genes

Abstract When cultured in axenic medium, Caenorhabditis elegans shows the largest life-span extension compared with other dietary restriction regimens. However, the underlying molecular mechanism still remains elusive. The gene cbp-1, encoding the worm ortholog of p300/CBP (CREB-binding protein), is one of the very few key genes known to be essential for life span doubling under axenic dietary restriction (ADR). By using tissue-specific RNAi, we found that cbp-1 expression in the germline is essential for fertility, whereas this gene functions specifically in the GABAergic neurons to support the full life span–doubling effect of ADR. Surprisingly, GABA itself is not required for ADR-induced longevity, suggesting a role of neuropeptide signaling. In addition, chemotaxis assays illustrate that neuronal inactivation of CBP-1 affects the animals’ food sensing behavior. Together, our results show that the strong life-span extension in axenic medium is under strict control of GABAergic neurons and may be linked to food sensing.

scholarly journals Age- and calorie-independent life span extension from dietary restriction by bacterial deprivation in Caenorhabditis elegans

2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Erica D Smith ◽  
Tammi L Kaeberlein ◽  
Brynn T Lydum ◽  
Jennifer Sager ◽  
K Linnea Welton ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Life Span ◽  
Dietary Restriction ◽  
Life Span Extension ◽  
Independent Life

scholarly journals The genetic paradigms of dietary restriction fail to extend life span in cep-1(gk138) mutant of C. elegans p53 due to possible background mutations

2020 ◽  
Author(s):  
Anita Goyala ◽  
Aiswarya Baruah ◽  
Arnab Mukhopadhyay
Keyword(s):  
Life Span ◽  
Dietary Restriction ◽  
Model Systems ◽  
Regulation Of Expression ◽  
Life Span Extension ◽  
Phenotypic Differences ◽  
C Elegans ◽  
Xenobiotic Detoxification ◽  
Organismal Biology ◽  
Extend Life Span

AbstractDietary restriction (DR) increases life span and improves health in most model systems tested, including non-human primates. In C. elegans, as in other models, DR leads to reprogramming of metabolism, improvements in mitochondrial health, large changes in gene expression, including increase in expression of cytoprotective genes, better proteostasis etc. Understandably, multiple global transcriptional regulators like transcription factors FOXO/DAF-16, FOXA/PHA-4, HSF1/HSF-1 and NRF2/SKN-1 are important for DR longevity. Considering the wide-ranging effects of p53 on organismal biology, we asked whether the C. elegans ortholog, CEP-1 is required for DR-mediated longevity assurance. We employed the widely-used TJ1 strain of cep-1(gk138). We show that cep-1(gk138) suppresses the life span extension of two genetic paradigms of DR, but two non-genetic modes of DR remain unaffected in this strain. We find that in cep-1(gk138), two aspects of DR, increased autophagy and the up-regulation of expression of cytoprotective xenobiotic detoxification program (cXDP) genes are dampened. Importantly, we find that background mutation(s) in the strain may be the actual cause for the phenotypic differences that we observed and cep-1 may not be directly involved in genetic DR-mediated longevity assurance in worms. Identifying these mutation(s) may reveal a novel regulator of longevity required specifically by genetic modes of DR.

scholarly journals Regulation of Life Span by The Gut Microbiota in The Short-Lived African Turquoise Killifish

2017 ◽  
Author(s):  
Patrick Smith ◽  
David Willemsen ◽  
Miriam Popkes ◽  
Franziska Metge ◽  
Edson Gandiwa ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Gut Microbiota ◽  
Life Span ◽  
Middle Age ◽  
External Environment ◽  
Systemic Effects ◽  
Life Span Extension ◽  
Nothobranchius Furzeri ◽  
Vertebrate Model

ABSTRACTGut bacteria occupy the interface between the organism and the external environment, contributing to homeostasis and disease. Yet, the causal role of the gut microbiota during host aging is largely unexplored. Here, using the African turquoise killifish (Nothobranchius furzeri), a naturally short-lived vertebrate, we show that the gut microbiota plays a key role in modulating vertebrate life span. Recolonizing the gut of middle-age individuals with bacteria from young donors resulted in life span extension and delayed behavioral decline. This intervention prevented the decrease in microbial diversity associated with host aging and maintained a young-like gut bacterial community, characterized by overrepresentation of the key genera Exiguobacterium, Planococcus, Propionigenium and Psychrobacter. Our findings demonstrate that the natural microbial gut community of young individuals can causally induce long-lasting beneficial systemic effects that lead to life span extension in a vertebrate model.

scholarly journals Lamin regulates the dietary restriction response via the mTOR pathway in Caenorhabditis elegans

2021 ◽  
Author(s):  
Chayki Charar ◽  
Sally Metsuyanim-Cohen ◽  
Daniel Z. Bar
Keyword(s):  
Body Size ◽  
Caenorhabditis Elegans ◽  
Life Span ◽  
Dietary Restriction ◽  
Metabolic Regulation ◽  
Fat Content ◽  
Mtor Pathway ◽  
Reduced Body ◽  
Animal Size ◽  
Mtor Complex 1

Animals subjected to dietary restriction (DR) have reduced body size, low fecundity, slower development, lower fat content and longer life span. We identified lamin as a regulator of multiple dietary restriction phenotypes. Downregulation of lmn-1, the single Caenorhabditis elegans lamin gene, increased animal size and fat content, specifically in DR animals. The LMN-1 protein acts in the mTOR pathway, upstream to RAPTOR and S6K, key component and target of mTOR complex 1 (mTORC1), respectively. DR excludes the mTORC1 activator RAGC-1 from the nucleus. Downregulation of lmn-1 restores RAGC-1 to the nucleus, a necessary step for the activation of the mTOR pathway. These findings further link lamin to metabolic regulation.

scholarly journals Hexane Extract of Rhizomes of Curcuma Longa, Zingiber Officinale and Curcumine Life Span Extension in Caenorhabditis Elegans By Reducing Fat in Intestine

Med Phoenix ◽  
2018 ◽  
Vol 3 (1) ◽  
pp. 21-28
Author(s):  
Parveen Gazala ◽  
Basavan Duraiswamy ◽  
Firoz Ansari
Keyword(s):  
Caenorhabditis Elegans ◽  
Life Span ◽  
Curcuma Longa ◽  
Zingiber Officinale ◽  
Intracellular Reactive Oxygen Species ◽  
Hexane Extract ◽  
Oxygen Species ◽  
Wild Type ◽  
Life Span Extension ◽  
Hexane Extracts

Background: Curcumine is obtained from curcuma longa and we examined the effects of curcumin, hexane extracts of Curcuma longa and Zingiber officinale on the lifespan and aging in Caenorhabditis elegans and found that it responded to curcumin, hexane extracts of Curcuma longa and Zingiber officinale with an increased lifespan and reduced intracellular reactive oxygen species during aging.Methods: MutantStrains, culture, Curcumin and Hexane extract of Rhizomes of Curcuma longa and Zingiber officinale treatment of C. Elegan.Results: Curcumin and hexane extracts of Curcuma longa and Zingiber officinale increased the life span and life cycle of the N2 wild type and Zdls-5 worms. On comparison, curcumin was found to be the most effective followed by the hexane extracts of Curcuma longa and hexane extracts of Zingiber officinale. Hexane extracts of Zingiber officinale were found to be least effective. Sudan black staining exhibited that stored contents of fat in C.elegans decreased as the concentration of the drug increased.Conclusions: Our study has established that curcumin and hexane extract of rhizomes of Curcuma longa and Zingiber officinale provides longevity and decreases the fat content in C.elegans.Med Phoenix. Vol. 3, Issue. 1, 2018, Page: 21-28                                            

scholarly journals 20S proteasome activation promotes life span extension and resistance to proteotoxicity in Caenorhabditis elegans

2014 ◽  
Vol 29 (2) ◽  
pp. 611-622 ◽  
Author(s):  
Niki Chondrogianni ◽  
Konstantina Georgila ◽  
Nikos Kourtis ◽  
Nektarios Tavernarakis ◽  
Efstathios S. Gonos
Keyword(s):  
Caenorhabditis Elegans ◽  
Life Span ◽  
20S Proteasome ◽  
Life Span Extension

scholarly journals Life-Span Extension From Hypoxia in Caenorhabditis elegans Requires Both HIF-1 and DAF-16 and Is Antagonized by SKN-1

2013 ◽  
Vol 68 (10) ◽  
pp. 1135-1144 ◽  
Author(s):  
Scott F. Leiser ◽  
Marissa Fletcher ◽  
Anisoara Begun ◽  
Matt Kaeberlein
Keyword(s):  
Caenorhabditis Elegans ◽  
Life Span ◽  
Life Span Extension
Sign in / Sign up

Export Citation Format

Share Document