Sperm motility index: a quick screening parameter from sperm quality analyser-IIB to rule out oligo- and asthenozoospermia in male fertility study

Abstract ObjectiveThis study aimed to reveal the protective effect of hydrogen storage nanomaterial MgH2 on radiation-induced male fertility impairment.MethodsThe characterization of MgH2 were analyzed by scanning electron microscopy (SEM) and particle size analyzer. The safety of MgH2 were evaluated in vivo and in vitro. The radioprotective effect of MgH2 on the reproductive system were analyzed in mice, including sperm quality, genetic effect, spermatogenesis, and hormone secretion. ESR, flow cytometry and western blotting assay were used to reveal the underlying mechanisms.ResultsMgH2 had an irregular spherical morphology and a particle size of approximately 463.2 nm, and the content of Mg reached 71.46%. MgH2 was safe and nontoxic in mice and cells. After irradiation, MgH2 treatment significantly protected testicular structure, increased sperm density, improved sperm motility, reduced deformity rates, and reduced the genetic toxicity. Particularly, the sperm motility were consistent with those in MH mice and human semen samples. Furthermore, MgH2 treatment could maintain hormone secretion and testicular spermatogenesis, especially the generation of Sertoli cells, spermatogonia and round sperm cells. In vitro, MgH2 eliminated the [·OH], suppressed the irradiation-induced increase in ROS production, and effectively alleviated the increase in MDA contents. Moreover, MgH2 significantly ameliorated apoptosis in testes and cells and reversed the G2/M phase cell cycle arrest induced by irradiation. In addition, MgH2 inhibited the activation of radiation-induced inflammation and pyroptosis.ConclusionMgH2 improved irradiation-induced male fertility impairment by eliminating hydroxyl free radicals.

Download Full-text

A Review on Environmental Contaminants-Related Fertility Threat in Male Fishes: Effects and Possible Mechanisms of Action Learned from Wildlife and Laboratory Studies

Animals ◽

10.3390/ani11102817 ◽

2021 ◽

Vol 11 (10) ◽

pp. 2817

Author(s):

Sayyed Mohammad Hadi Alavi ◽

Sepideh Barzegar-Fallah ◽

Parastoo Rahdar ◽

Mohammad Mahdi Ahmadi ◽

Mina Yavari ◽

...

Keyword(s):

Sperm Motility ◽

Reproductive System ◽

Male Fertility ◽

Developmental Stages ◽

Sperm Quality ◽

Environmental Contaminants ◽

Laboratory Studies ◽

Reproductive Disorders ◽

Synthetic Hormones ◽

Early Developmental

Increasing global rates of diminished fertility in males has been suggested to be associated with exposure to environmental contaminants (ECs). The aquatic environments are the final repository of ECs. As the reproductive system is conserved in vertebrates, studies on the effects of ECs on fertility endpoints in fishes provide us with valuable information to establish biomarkers in risk assessment of ECs, and to understand the ECs-related fertility threat. The aim of the present review was to evaluate associations between ECs and fertility determinants to better understand ECs-related male fertility threat in male fishes. Wildlife studies show that the reproductive system has been affected in fishes sampled from the polluted aquatic environment. The laboratory studies show the potency of ECs including natural and synthetic hormones, alkylphenols, bisphenols, plasticizers, pesticides, pharmaceutical, alkylating, and organotin agents to affect fertility determinants, resulting in diminished fertility at environmentally relevant concentrations. Both wildlife and laboratory studies reveal that ECs adverse effects on male fertility are associated with a decrease in sperm production, damage to sperm morphology, alternations in sperm genome, and decrease in sperm motility kinetics. The efficiency of ECs to affect sperm quality and male fertility highly depends on the concentration of the contaminants and the duration of exposure. Our review highlights that the number of contaminants examined over fertility tests are much lower than the number of contaminants detected in our environment. The ECs effects on fertility are largely unknown when fishes are exposed to the contaminants at early developmental stages. The review suggests the urgent need to examine ECs effects on male fertility when a fish is exposed at different developmental stages in a single or combination protocol. The ECs effects on the sperm genome are largely unknown to understand ECs-related inheritance of reproductive disorders transmitted to the progeny. To elucidate modes of action of ECs on sperm motility, it is needed to study functional morphology of the motility apparatus and to investigate ECs-disrupted motility signaling.

Download Full-text

Effect of Lunasia amara Blanco on Sperm Number, Sperm Motility, and Testicular Histology of Male Rats

Biology Medicine & Natural Product Chemistry ◽

10.14421/biomedich.2015.42.31-33 ◽

2015 ◽

Vol 4 (2) ◽

pp. 31

Author(s):

Muhammad Ja’far Luthfi

Keyword(s):

Sperm Motility ◽

Male Fertility ◽

Sperm Quality ◽

Sperm Count ◽

Male Rats ◽

Quality Analysis ◽

Sprague Dawley ◽

Sperm Analysis ◽

Testicular Histology ◽

Positive Effect

<p>Sanrego (<em>Lunasia amara</em>), has been used in the folk medicine to increase and/or to treat male fertility. However there is no scientific evidence to confirm the positive effect of the plant on an improvement of male fertility. The objective of this research was to study the effects of the plant (on adult Sprague-Dawley male rats) at the doses of 30 mg/kg, 60 mg/kg, and 90 mg/kg on the sperm count, motility, and testicular histology. Administration were given by force-feeding between 10.00 am and 12.00 pm daily for a period of 42 days followed by sperm quality analysis and testicular histology evaluation. The sperm analysis showed that the sanrego increased the sperm count and sperm motility. The testicular histology also revealed positive effect of the plant on spermatogenesis. Overall the present study showed the sanrego is potential plant to increase male fertility.</p>

Download Full-text

Post-coital sperm recovery and cryopreservation in the Sumatran rhinoceros (Dicerorhinus sumatrensis) and application to gamete rescue in the African black rhinoceros (Diceros bicornis)

Reproduction ◽

10.1530/reprod/118.2.263 ◽

2000 ◽

pp. 263-271 ◽

Cited By ~ 15

Author(s):

JK O'Brien ◽

TL Roth

Keyword(s):

Sperm Motility ◽

Artificial Insemination ◽

Sperm Quality ◽

Glass Wool ◽

Motility Index ◽

Black Rhinoceros ◽

Diceros Bicornis ◽

Fluorescent Markers ◽

Acceptable Quality ◽

African Black

Sumatran rhinoceros (Dicerorhinus sumatrensis) sperm samples were collected from a post-copulatory female and characterized to determine their potential for sperm preservation and future use in artificial insemination. Five samples of acceptable quality from one male were used to compare the effect of two cryoprotectants (glycerol and dimethyl sulfoxide (DMSO)) and two post-thaw protocols (untreated and glass wool column) on sperm quality. The percentage of motile spermatozoa, sperm motility index (0-100) and sperm morphology were evaluated subjectively, and viability and acrosomal status were assessed using fluorescent markers. Evaluations of frozen-thawed spermatozoa were performed over a 6 h incubation interval. Post-coital semen samples (n = 5; 104.0 +/- 9.1 ml; 2.5 +/- 0.8 x 10(9) total spermatozoa; mean +/- SEM) exhibited a sperm motility index of 56.7 +/- 3.3, and contained 40.2 +/- 6.3%, 72.0 +/- 3.2% and 79.8 +/- 6.5% normal, viable and acrosome-intact spermatozoa, respectively. Glycerol and DMSO were equally effective as cryoprotectants and, regardless of post-thaw protocol, samples retained greater than 80% of all pre-freeze characteristic values. Processing semen samples through glass wool yielded higher quality samples, but only half the total number of motile spermatozoa compared with untreated samples. High values for pre-freeze sperm characteristics were also maintained after cryopreservation of epididymal spermatozoa from one black rhinoceros (Diceros bicornis) using the same protocol. In summary, Sumatran rhinoceros spermatozoa of moderate quality can be collected from post-copulatory females. Rhinoceros sperm samples show only slight reductions in quality after cryopreservation and thawing and have potential for use in artificial insemination.

Download Full-text

Measurement of the sperm motility index via the sperm quality analyzer and its relationship to other qualitative sperm parameters

Theriogenology ◽

10.1016/0093-691x(96)00164-1 ◽

1996 ◽

Vol 46 (3) ◽

pp. 421-427 ◽

Cited By ~ 7

Author(s):

P.M. Zavos ◽

J.R. Correa ◽

P.N. Zarmakoupis-Zavos

Keyword(s):

Sperm Motility ◽

Sperm Quality ◽

Sperm Parameters ◽

Motility Index ◽

Quality Analyzer ◽

Sperm Quality Analyzer

Download Full-text

Determinants of infertility in couples and methods for their correction

Voprosy ginekologii akušerstva i perinatologii ◽

10.20953/1726-1678-2021-1-105-112 ◽

2021 ◽

Vol 20 (1) ◽

pp. 105-112

Author(s):

E.V. Shikh ◽

◽

A.A. Makhova ◽

E.D. Khaytovich ◽

◽

...

Keyword(s):

Sperm Motility ◽

Male Fertility ◽

Sperm Quality ◽

Sperm Count ◽

Sperm Concentration ◽

Added Sugar ◽

Trans Fats ◽

Factors Affecting ◽

Micronutrient Intake ◽

Positive Effect

Nutrition is one of the main environmental factors affecting the reproductive system. The modern diet is characterized by higher consumption of high-calorie foods, saturated fats, trans fats, refined grains and added sugar. Despite the heterogeneity of the populations studied, in general, the data confirm a steady downward trend in male sperm quality in most Western countries, including an increase in the prevalence of conditions such as complete asthenozoospermia (lack of motility) and isolated asthenozoospermia (low sperm motility due to varicocele, infections or genetic factors). Clear patterns between nutrition and fertility have been identified. Studies have shown a significant positive effect of additional exogenous micronutrient intake on total sperm count (ω-3 and CoQ10); sperm concentration (selenium, zinc, ω-3 and CoQ10); sperm motility (selenium, zinc, ω-3, CoQ10 and carnitine); morphology (selenium, ω-3, CoQ10 and carnitine). Additional exogenous intake of micronutrients that affect sperm can be used as an effective and safe factor in modulating male fertility. Key words: coenzyme Q10, lipoic acid, male fertility, selenium, sperm, zinc

Download Full-text

Effect of new dietary supplement on sperm quality

Orvosi Hetilap ◽

10.1556/oh.2012.29468 ◽

2012 ◽

Vol 153 (45) ◽

pp. 1787-1792 ◽

Cited By ~ 1

Author(s):

Mária Horváth ◽

Endre Czeizel

Keyword(s):

Adverse Effect ◽

Clinical Trial ◽

Dietary Supplement ◽

Male Fertility ◽

Sperm Quality ◽

Sperm Count ◽

Before And After ◽

The Usa ◽

New Treatments

Introduction: There is a decline in male fertility thus new treatments are needed. Aims: To test the efficacy of a new dietary supplement developed in the USA and registered as a curing drug in Hungary (OGYI). Methods: In a clinical trial 100 men with low sperm quality (spermium count 5–20 M/ml, good motility 10–40%, and adverse shape 30–50%) were examined. Results: Sperm parameters were measured before and after a 3-month treatment and after another 3-month without treatment. This dietary supplement statistically and clinically significantly improved sperm count and motility. In 74 cases this dietary supplement demonstrated a beneficial effect on sperm quality (more than 10% increase in sperm count, or quality of motility, or shape); in 16 cases the improvement exceeded 30%. No adverse effect could be accounted for this treatment. Conclusions: This new dietary supplement may contribute to the treatment of male infertility. Orv. Hetil., 2012, 153, 1787–1792.

Download Full-text

Faculty Opinions recommendation of A sperm ion channel required for sperm motility and male fertility.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1002013.18956 ◽

2001 ◽

Author(s):

Roger Hardie

Keyword(s):

Ion Channel ◽

Sperm Motility ◽

Male Fertility

Download Full-text

CagA-PositiveHelicobacter pyloriInfection and Reduced Sperm Motility, Vitality, and Normal Morphology

Disease Markers ◽

10.1155/2013/919174 ◽

2013 ◽

Vol 35 ◽

pp. 229-234 ◽

Cited By ~ 8

Author(s):

E. Moretti ◽

G. Collodel ◽

L. Mazzi ◽

M. S. Campagna ◽

N. Figura

Keyword(s):

Sperm Motility ◽

Normal Forms ◽

Molecular Mimicry ◽

Sperm Quality ◽

Semen Analysis ◽

Normal Morphology ◽

Partial Linear ◽

Caga Status ◽

The Relationship ◽

Autoimmune Phenomena

Helicobacter pylori(HP) infection, particularly when caused by strains expressing CagA, may be considered a concomitant cause of male and female reduced fertility. This study explored, in 87 HP-infected males, the relationship between infection by CagA-positive HP strains and sperm parameters. HP infection and CagA status were determined by ELISA and Western blotting; semen analysis was performed following WHO guidelines. The amino acid sequence of human enzymes involved in glycolysis and oxidative metabolism were “blasted” with peptides expressed by HP J99. Thirty-seven patients (42.5%) were seropositive for CagA. Sperm motility (18% versus 32%; ), sperm vitality (35% versus 48%; ) and the percentage of sperm with normal forms (18% versus 22%; ) in the CagA-positive group were significantly reduced versus those in the CagA-negative group. All the considered enzymes showed partial linear homology with HP peptides, but four enzymes aligned with four different segments of the samecagisland protein. We hypothesize a relationship between infection by strains expressing CagA and decreased sperm quality. Potentially increased systemic levels of inflammatory cytokines that occur in infection by CagA-positive strains and autoimmune phenomena that involve molecular mimicry could explain the pathogenetic mechanism of alterations observed.

Download Full-text