Post-coital sperm recovery and cryopreservation in the Sumatran rhinoceros (Dicerorhinus sumatrensis) and application to gamete rescue in the African black rhinoceros (Diceros bicornis)

Reproduction ◽  
2000 ◽  
pp. 263-271 ◽  
Author(s):  
JK O'Brien ◽  
TL Roth
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Sperm Quality ◽  
Glass Wool ◽  
Motility Index ◽  
Black Rhinoceros ◽  
Diceros Bicornis ◽  
Fluorescent Markers ◽  
Acceptable Quality ◽  
African Black

Sumatran rhinoceros (Dicerorhinus sumatrensis) sperm samples were collected from a post-copulatory female and characterized to determine their potential for sperm preservation and future use in artificial insemination. Five samples of acceptable quality from one male were used to compare the effect of two cryoprotectants (glycerol and dimethyl sulfoxide (DMSO)) and two post-thaw protocols (untreated and glass wool column) on sperm quality. The percentage of motile spermatozoa, sperm motility index (0-100) and sperm morphology were evaluated subjectively, and viability and acrosomal status were assessed using fluorescent markers. Evaluations of frozen-thawed spermatozoa were performed over a 6 h incubation interval. Post-coital semen samples (n = 5; 104.0 +/- 9.1 ml; 2.5 +/- 0.8 x 10(9) total spermatozoa; mean +/- SEM) exhibited a sperm motility index of 56.7 +/- 3.3, and contained 40.2 +/- 6.3%, 72.0 +/- 3.2% and 79.8 +/- 6.5% normal, viable and acrosome-intact spermatozoa, respectively. Glycerol and DMSO were equally effective as cryoprotectants and, regardless of post-thaw protocol, samples retained greater than 80% of all pre-freeze characteristic values. Processing semen samples through glass wool yielded higher quality samples, but only half the total number of motile spermatozoa compared with untreated samples. High values for pre-freeze sperm characteristics were also maintained after cryopreservation of epididymal spermatozoa from one black rhinoceros (Diceros bicornis) using the same protocol. In summary, Sumatran rhinoceros spermatozoa of moderate quality can be collected from post-copulatory females. Rhinoceros sperm samples show only slight reductions in quality after cryopreservation and thawing and have potential for use in artificial insemination.

scholarly journals Use of a sperm analyzer for evaluating broiler breeder males. 1. Effects of altering sperm quality and quantity on the sperm motility index

1998 ◽  
Vol 77 (6) ◽  
pp. 888-893 ◽  
Author(s):  
CD McDaniel ◽  
JL Hannah ◽  
HM Parker ◽  
TW Smith ◽  
CD Schultz ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Motility Index ◽  
Broiler Breeder ◽  
Broiler Breeder Males

scholarly journals Fertility and Sperm Quality of 1- and 2-year-Old Native Ganders Used for Natural Mating and Artificial Insemination

2021 ◽  
Author(s):  
Mehmet Akif BOZ ◽  
Hatice BAŞ ◽  
Musa SARICA ◽  
Kadir ERENSOY
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Egg Production ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Day Length ◽  
Quality Traits ◽  
Female Ratio ◽  
Semen Volume ◽  
Natural Mating

Abstract In the present study, the fertility rate and egg-hatching results from either natural mating or artificial insemination using 1- and 2-year-old domestic Turkish geese were compared. Sperm quality traits of 1- and 2-year-old ganders used for mating were determined. The study comprised 72 two-year-old females, 12 one-year-old, and 12 two-year-old ganders at the beginning of the laying period. Thirty-six female geese were mated naturally (18 with 1-year-old ganders, 18 with 2-year-old ganders); the remaining 36 were artificially inseminated (18 with 1-year-old ganders, 18 with 2-year-old ganders). Twenty-four ganders were separated into groups (12 for natural mating; 12 for artificial insemination). The male-to-female ratio in both mating protocols was 1:3. The geese were housed during the laying period in a natural and artificially ventilated house under natural lighting (increased day length). Quality traits, such as semen volume, sperm concentration, sperm quality factor (SQF), sperm motility, and some sperm morphological properties were determined. Fertility was higher in the groups containing 2-year-old ganders than in those using 1-year-old ganders (P < 0.05). The effect of insemination on semen volume, sperm concentration, SQF, sperm motility, total live sperm, normal sperm, macrocephalus sperm, and dead sperm was determined to be significant (P < 0.05). As a result, there is a need to improve the egg production and broody behavior traits of domestic Turkish geese. In addition, the use of 2-year-old geese in artificial insemination is appropriate and will contribute positively to the breeding and selection process.

CHANGES DURING LACTATION IN THE COMPOSITION OF THE MILK OF THE AFRICAN BLACK RHINOCEROS (DICEROS BICORNIS)

2009 ◽  
Vol 145 (3) ◽  
pp. 327-333 ◽  
Author(s):  
MARGARET E. GREGORY ◽  
S. J. ROWLAND ◽  
S. Y. THOMPSON ◽  
VANDA M. KON
Keyword(s):  
Black Rhinoceros ◽  
Diceros Bicornis ◽  
African Black

scholarly journals Rectal prolapse associated with a healed pelvic fracture in a pregnant free-ranging African black rhinoceros (Diceros bicornis). Part 1 : anaesthesia : case report

2001 ◽  
Vol 72 (4) ◽  
Author(s):  
G.F. Stegmann ◽  
M. Hofmeyr ◽  
A. Olivier ◽  
E. Lane ◽  
D.H. Volkmann
Keyword(s):  
Rectal Prolapse ◽  
Tidal Volume ◽  
Venous Blood ◽  
Blood Gas Analysis ◽  
Gas Analysis ◽  
Muscle Rigidity ◽  
Black Rhinoceros ◽  
Diceros Bicornis ◽  
African Black ◽  
Free Ranging

Anaesthesia was required in a heavily-pregnant, adult, free-ranging African black rhinoceros Diceros bicornis with a rectal prolapse for examination and possible treatment. The animal was immobilised with 4.5 mg etorphine and 60 mg azaperone. For continued observation, the immobilised animal was transported to a boma. Additional etorphine and azaperone were administered to keep the animal anaesthetised during treatment and transport. In addition, 15 mg nalorphine was administered during this time to improve ventilation and reduce muscle rigidity. Sixty hours later, in preparation for surgery, 2.5 mg etorphine and 40 mg azaperone were administered, followed by endotracheal intubation and halothane anaesthesia. During anaesthesia, a decrease in tidal volume was observed. Venous blood-gas analysis indicated a decrease in the oxygen partial pressure, and a mixed respiratory and metabolic acidosis. Cardiac arrest was preceded by an increase in heart rate and tidal volume after 80 min of inhalation anaesthesia.

THE COMPOSITION OF THE MILK OF THE AFRICAN BLACK RHINOCEROS (DICEROS BICORNIS; LINN.)

2009 ◽  
Vol 137 (3) ◽  
pp. 475-479 ◽  
Author(s):  
R. ASCHAFFENBURG ◽  
MARGARET E. GREGORY ◽  
S. J. ROWLAND ◽  
S. Y. THOMPSON ◽  
VANDA M. KON
Keyword(s):  
Black Rhinoceros ◽  
Diceros Bicornis ◽  
African Black

scholarly journals Umur Memengaruhi Volume Semen dan Motilitas Spermatozoa Babi Landrace di Balai Inseminasi Buatan Baturiti, Tabanan, Bali (AGE AFFECTS SEMEN VOLUME AND MOTILITY OF SPERMATOZOA LANDRACE BOAR’S OF BATURITI ARTIFICIAL INSEMINATION CENTER, TABANAN, BALI)

2019 ◽  
Vol 20 (3) ◽  
pp. 324
Author(s):  
Ni Luh Gde Sumardani ◽  
Komang Budaarsa ◽  
Tjok Istri Putri ◽  
Antonius Wayan Puger
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Light Microscope ◽  
Sperm Quality ◽  
Volume Average ◽  
Randomized Design ◽  
Glass Slides ◽  
Semen Volume ◽  
Research Show

Sperm quality have been associated with age for many years. This study aims to determine the inffluence of age to the volume and motility of Landrace boar’s sperm at Baturiti Artificial Insemination (AI) center. A Total of 300 ejaculates were used in this study. An ejaculate origin from five boar are a collection of five month. A complete randomized design (CRD) was used with two different boar of block ages, block A (2-4 year) and block B (6-8 year). Three or four glass slides were prepared for each boar sample; a drop of semen was placed on each glass slides. This sample was examined under the light microscope on five view field in each glass slides. The result of the research show that the Landrace boars in block A have semen volume average 273.60 mL and sperm motility 73.86%, while in block B the average semen volume was 107.66 mL and sperm motility 62.92%. It can be concluded that Landrace boars in block A had higher volume and sperm motility compared to Landrace boars in block B.

scholarly journals Improving the Rabbit Semen Cryopreservation Protocol: Comparison Between Two Extenders and Inseminating Doses

2020 ◽  
Vol 20 (3) ◽  
pp. 887-898
Author(s):  
Michele Di Iorio ◽  
Giusy Rusco ◽  
Maria Antonietta Colonna ◽  
Michele Schiavitto ◽  
Maria Silvia D’Andrea ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Artificial Insemination ◽  
Sperm Quality ◽  
Dna Integrity ◽  
Semen Cryopreservation ◽  
Fertilizing Ability ◽  
Cryopreservation Protocol

AbstractThis study has been designed to optimize the semen freezing protocol in rabbits, in this regard we compared a Tris-citrate-glucose (TCG) extender with a commercial one (Cortalap®), that to the best of our knowledge has never been used up to now on the in vitro freezability and fertilizing ability of cryopreserved rabbit semen. Two different inseminating semen doses were considered. Five pooled semen samples were divided into two subsamples and each of them were diluted to a ratio 1:1 (v:v) with a freezing extender composed of TCG or Cortalap® containing 16% of dimethylsulfoxide and 0.1 mol/L of sucrose. The extended semen was filled into 0.25 mL plastic straws and frozen above a liquid nitrogen surface. After thawing (50°C/10 seconds) we determined sperm motility, viability, membrane functionality, acrosome and DNA integrity. Our results showed that the Cortalap® extender significantly improved the in vitro post-thaw sperm quality, in comparison to TCG. When we compared the extenders in vivo, no significant differences in the reproductive performances were observed independently by inseminating doses used. In this study we demonstrated that Cortalap® extender can be used as an alternative to TCG. Thus, the Cortalap® being a ready to use extender, implies a reduction of time, mistakes and microbial contaminations during its preparation. This discovery results as significant because it provides beyond an important contribution to the creation of the first Italian semen cryobank of rabbit breeds and also for livestock rabbit farms based on artificial insemination (AI) program.

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