P–153 Comparison outcome of vitrified human embryos stored in vapor phase liquid nitrogen (LN2) and direct LN2

Abstract Study question Is vapor cryopreserved LN2 storage beneficial for clinical outcomes of vitrified human embryos that are frozen compared to vitrified human embryos having direct contact with LN2. Summary answer There are no significant differences compared to clinical outcomes of human embryos stored in LN2 vapor and direct store in LN2. What is known already There has been concerned about potential cross-contamination and biohazard issues of embryos for long term storage using direct LN2. This study aimed to compare clinical outcomes of human embryos transfer between vapor phase and liquid LN2. Study design, size, duration The embryo has undergone vitrification for long term storage with vapor or direct contact in LN2. After the thawing of the embryo, we checked on the survival rates. We transferred only one or two embryos per patient and kept analyzing the implantation and pregnancy rates Participants/materials, setting, methods This retrospective study was carried out from January 2018 to December 2019 with 3272cycles 4713embryos; vitrified for long term storage in vapor phase or direct contact with LN2. We compared the clinical outcomes of frozen embryo transfer cycles using vitrified for long term storage in vapor phase and direct contact with LN2. Clinical outcomes monitored were embryo survival, subsequent implantation and pregnancy after single or double embryo transfer Main results and the role of chance A total of 4713 fertilized human embryos are vitrified and then stored in LN2 vapor (n = 2520 cycles) or direct contact LN2 (n = 752 cycles). The study showed that the blastocyst stored in vapor able to retain full development. Survival was 97.8% (vapor) and 97.6% (direct contact LN2), and the vapor storage of human embryos had no significant difference in survival rates after a long term storage. For single blastocyst transfer, pregnancy and implantation rates were 51.5%, 52.4% in vapor, 54.6%, 54.9% in direct LN2; respectively (p=NS). In double blastocyst transfer, the pregnancy and implantation rates were 61.8%, 42.0% in vapor and 64.7%, 44.5% in direct LN2; respectively (p=NS). There were also no significant differences between two groups. Limitations, reasons for caution The study showed that the blastocyst stored in vapor can retain full development. A vapor storage system thus is safe and effective for long term vapor storage of vitrified human embryos.Within the limits of this study, there was no detection of an adverse effect of vapor storage. Wider implications of the findings: Vapor storage systems thus represent a useful alternative for safe and effective long-term storage of vitrified human embryos that can avoid cross contamination chances from having direct contact with LN2. Trial registration number Not applicable

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61. Comparison of a mechanical (−150°C) refrigerator with the vapor phase over liquid nitrogen (<−170°C) for the long-term storage of human peripheral blood stem cells

Cryobiology ◽

10.1016/j.cryobiol.2011.09.064 ◽

2011 ◽

Vol 63 (3) ◽

pp. 322-323

Author(s):

Andreas Sputtek ◽

Evgeny Klyuchnikov ◽

Nikolaus Kröger ◽

Sven Peine ◽

Arthur W Rowe

Keyword(s):

Stem Cells ◽

Liquid Nitrogen ◽

Vapor Phase ◽

Peripheral Blood ◽

Human Peripheral Blood ◽

Peripheral Blood Stem Cells ◽

Long Term Storage ◽

Blood Stem Cells ◽

Term Storage

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Human embryos and eggs: from long-term storage to biobanking

Bioethics News ◽

10.1007/s40592-015-0045-8 ◽

2015 ◽

Vol 33 (4) ◽

pp. 340-359 ◽

Cited By ~ 4

Author(s):

Françoise Baylis ◽

Heather Widdows

Keyword(s):

Human Embryos ◽

Long Term Storage ◽

Term Storage

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Microbial occurrence in liquid nitrogen storage tanks: a challenge for cryobanking?

Applied Microbiology and Biotechnology ◽

10.1007/s00253-021-11531-4 ◽

2021 ◽

Author(s):

Felizitas Bajerski ◽

Manuela Nagel ◽

Joerg Overmann

Keyword(s):

Risk Assessment ◽

Quality Management ◽

Liquid Nitrogen ◽

Potential Risk ◽

Biological Materials ◽

Cross Contamination ◽

Storage Tanks ◽

Long Term Storage ◽

Term Storage

Abstract Modern biobanks maintain valuable living materials for medical diagnostics, reproduction medicine, and conservation purposes. To guarantee high quality during long-term storage and to avoid metabolic activities, cryostorage is often conducted in the N2 vapour phase or in liquid nitrogen (LN) at temperatures below − 150 °C. One potential risk of cryostorage is microbial cross contamination in the LN storage tanks. The current review summarises data on the occurrence of microorganisms that may compromise the safety and quality of biological materials during long-term storage. We assess the potential for the microbial contamination of LN in storage tanks holding different biological materials based on the detection by culture-based and molecular approaches. The samples themselves, the LN, the human microbiome, and the surrounding environment are possible routes of contamination and can cause cross contaminations via the LN phase. In general, the results showed that LN is typically not the source of major contaminations and only a few studies provided evidence for a risk of microbial cross contamination. So far, culture-based and culture-independent techniques detected only low amounts of microbial cells, indicating that cross contamination may occur at a very low frequency. To further minimise the potential risk of microbial cross contaminations, we recommend reducing the formation of ice crystals in cryotanks that can entrap environmental microorganisms and using sealed or second sample packing. A short survey demonstrated the awareness for microbial contaminations of storage containers among different culture collections. Although most participants consider the risk of cross contaminations in LN storage tanks as low, they prevent potential contaminations by using sealed devices and − 150 °C freezers. It is concluded that the overall risk for cross contaminations in biobanks is relatively low when following standard operating procedures (SOPs). We evaluated the potential sources in detail and summarised our results in a risk assessment spreadsheet which can be used for the quality management of biobanks. Key points • Identification of potential contaminants and their sources in LN storage tanks. • Recommendations to reduce this risk of LN storage tank contamination. • Development of a risk assessment spreadsheet to support quality management.

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Long term storage and the compensatory growth of white shrimp Litopenaeus vannamei in aquaculture ponds

Latin American Journal of Aquatic Research ◽

10.3856/vol44-issue3-fulltext-17 ◽

2017 ◽

Vol 44 (3) ◽

pp. 588-594

Author(s):

Geraldo Fóes ◽

Dariano Krummenauer ◽

Gabriele Lara ◽

Luis Poersch ◽

Wilson Wasielesky Jr.

Keyword(s):

Compensatory Growth ◽

Management Practices ◽

Specific Growth ◽

Survival Rates ◽

White Shrimp ◽

Growth And Survival ◽

Conventional Management ◽

Long Term Storage ◽

Term Storage

Effects of shrimp confinement in a situation of high density stocking in a long term nursery on their growth performance in grow out ponds. Were analized two nurseries with a density of 2000 shrimp m-2 were stocked at two different times. The first nursery (LTN) lasted 144 days, and the SGR of the animals was 3.0% day-1. The second nursery (STN) lasted 18 days and the specific growth rate (SGR) was 19.9% day-1. On the same day, shrimps were transferred to six lined ponds at a density of 20 shrimp m2 where they remained for 101 days. In the first biometry, the SGR in the LTN treatment, increased to 6.7% day-1 and in the STN it decreased to 5.0% day-1. At the end, shrimps of the LTN and STN treatments reached weights of 8.46 and 6.72 g and had productivities of 1287 and 1015 kg ha-1, respectively. Shrimps reared in nurseries for long periods showed growth and survival rates similar to those obtained using conventional management practices in grow out structures.

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Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure

Horticultural Science ◽

10.17221/234/2013-hortsci ◽

2014 ◽

Vol 41 (No. 2) ◽

pp. 55-63 ◽

Cited By ~ 2

Author(s):

Dj. Ružić ◽

T. Vujović ◽

R. Cerović

Keyword(s):

Sucrose Concentration ◽

Survival Rates ◽

Shoot Tips ◽

Prunus Cerasus ◽

Ms Medium ◽

Long Term Storage ◽

Term Storage ◽

Gisela 5

In vitro-grown shoot tips of Gisela 5 (Prunus cerasus × Prunus canescens) cherry rootstock were tested for regrowth after cryopreservation using vitrification technique. Explants were precultured in the dark at 23°C, in a liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in a solution containing 2 M glycerol and 0.4 M sucrose for 20 minutes. Shoot tips were dehydrated at 0°C using either the original PVS2 or modified PVS2 solution (PVS A3 – 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30, 40 and 50 minutes. The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 36–54% and 8–17%, respectively. However, the dehydration with the PVS A3 solution resulted in considerably higher survival rates (81–92%), as well as higher regrowth rates (39–56%) after cryopreservation. These results prove the feasibility of the PVS A3-based vitrification technique for a long-term storage of this genotype.  

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Modelling of Moisture Movement in Wood during Outdoor Storage

Nonlinear Analysis Modelling and Control ◽

10.15388/na.2001.6.1.15210 ◽

2001 ◽

Vol 6 (2) ◽

pp. 3-14 ◽

Cited By ~ 23

Author(s):

R. Baronas ◽

F. Ivanauskas ◽

I. Juodeikienė ◽

A. Kajalavičius

Keyword(s):

Experimental Data ◽

Finite Difference ◽

Climatic Conditions ◽

Two Dimensional ◽

Moisture Movement ◽

Finite Difference Technique ◽

Long Term Storage ◽

Space Formulation ◽

Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

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