scholarly journals A protein isolated from human oviductal tissue in vitro secretion, identified as human lactoferrin, interacts with spermatozoa and oocytes and modulates gamete interaction

2013 ◽  
Vol 28 (5) ◽  
pp. 1297-1308 ◽  
Author(s):  
C. M. Zumoffen ◽  
R. Gil ◽  
A. M. Caille ◽  
C. Morente ◽  
M. J. Munuce ◽  
...  
Keyword(s):  
Human Lactoferrin ◽  
Gamete Interaction

Influence of the duration of gamete interaction on cleavage, growth rate and sex distribution of in vitro produced bovine embryos

2003 ◽  
Vol 77 (1-2) ◽  
pp. 33-49 ◽  
Author(s):  
Harpreet S Kochhar ◽  
Kanwal P Kochhar ◽  
Parvathi K Basrur ◽  
W.Allan King
Keyword(s):  
Growth Rate ◽  
Bovine Embryos ◽  
Sex Distribution ◽  
Gamete Interaction

scholarly journals The Synthetic N-Terminal Peptide of Human Lactoferrin, hLF(1-11), Is Highly Effective against Experimental Infection Caused by Multidrug-Resistant Acinetobacter baumannii

2004 ◽  
Vol 48 (12) ◽  
pp. 4919-4921 ◽  
Author(s):  
Lenie Dijkshoorn ◽  
Carlo P. J. M. Brouwer ◽  
Sylvia J. P. Bogaards ◽  
Alexandr Nemec ◽  
Peterhans J. van den Broek ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Experimental Infection ◽  
Multidrug Resistant ◽  
Human Lactoferrin ◽  
Promising Candidate ◽  
Log Reduction ◽  
Control Peptide ◽  
Highly Effective ◽  
Novel Agent

ABSTRACT The lactoferrin-derived peptide hLF(1-11), but not its control peptide, was highly effective against five multidrug-resistant Acinetobacter baumannii strains in vitro (3 to 4 log reduction) and against four of these strains in an experimental infection in mice (2 to 3 log reduction). Therefore, this peptide is a promising candidate as a novel agent against infections with multidrug-resistant A. baumannii.

scholarly journals Modulation of In Vitro Fungicidal Activity of Human Lactoferrin against Candida albicans by Extracellular Cation Concentration and Target Cell Metabolic Activity

2004 ◽  
Vol 48 (4) ◽  
pp. 1242-1248 ◽  
Author(s):  
Mónica Viejo-Díaz ◽  
María T. Andrés ◽  
José F. Fierro
Keyword(s):  
Candida Albicans ◽  
Divalent Cations ◽  
Human Lactoferrin ◽  
Killing Effect ◽  
Innate Defense ◽  
Mitochondrial Inhibitors ◽  
Carbonyl Cyanide ◽  
Defense Protein ◽  
Low Strength

ABSTRACT The anti-Candida activity of the innate defense protein human lactoferrin was investigated. Lactoferrin displayed a clear fungicidal effect against Candida albicans only under low-strength conditions. This candidacidal activity was inversely correlated with the extracellular concentration of the monovalent cations and was prevented by Na+ and K+ (≥30 mM) and by divalent cations (Ca2+ and Mg2+ at ≥4 mM). A slight cellular release of K+, cytosolic acidification, and a change in the membrane potential were observed in C. albicans cells treated with lactoferrin, suggesting that this protein directly or indirectly interacts with the cytoplasmic membrane. Mitochondrial inhibitors (carbonyl cyanide m-chlorophenylhydrazone, 2,4-dinitrophenol, azide, and antimycin) as well as anaerobic conditions significantly reduced the killing effect of lactoferrin. These results suggest that low-strength conditions and the cellular metabolic state may modulate the candidacidal activity of human lactoferrin.

Studies of the ceruloplasmin-lactoferrin complex

2002 ◽  
Vol 80 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Maria O Pulina ◽  
Elena T Zakharova ◽  
Alexei V Sokolov ◽  
Mikhail M Shavlovski ◽  
Mikhail G Bass ◽  
...  
Keyword(s):  
Breast Milk ◽  
Human Plasma ◽  
Bacterial Lipopolysaccharide ◽  
Human Lactoferrin ◽  
Present Evidence ◽  
High Concentrations

We have previously shown that iron-containing human lactoferrin (LF) purified from breast milk is able to form both in vitro and in vivo a complex with ceruloplasmin (CP), the copper-containing protein of human plasma. Here we present evidence that the CP–LF complex is dissociated by high concentrations of NaCl, CaCl2, or EDTA, or by decreasing the pH to 4.7. In addition, DNA, bacterial lipopolysaccharide, and heparin can displace CP from its complex with LF. Antibodies to either of the two proteins also cause dissociation of the complex.Key words: lactoferrin, ceruloplasmin, ferroxidase.

Effect of lactoferrin onHelicobacter felisinduced gastritis

2002 ◽  
Vol 80 (1) ◽  
pp. 113-117 ◽  
Author(s):  
Elizabeth J Dial ◽  
Lenard M Lichtenberger
Keyword(s):  
Surface Hydrophobicity ◽  
Human Lactoferrin ◽  
Bovine Lactoferrin ◽  
Recombinant Human Lactoferrin ◽  
Beneficial Effects ◽  
In Vivo Testing ◽  
H Pylori ◽  
Orally Active

Lactoferrin possesses antibiotic, antiinflammatory, and immune-modulating properties that may be active against the gastritis-, ulcer- and cancer-inducing bacterium Helicobacter pylori. In vitro testing of bovine and human lactoferrin by several laboratories has shown significant bacteriostatic and bactericidal activity. Subsequent in vivo testing of bovine lactoferrin in animal models of H. pylori infection has shown beneficial effects of this agent. Our laboratory has utilized a mouse model that is infected with the feline strain of this bacterium, H. felis. The resulting gastritis that develops in this model and the effects of bovine lactoferrin and recombinant human lactoferrin (from Aspergillus niger var. awamori, Agennix Inc., Houston, Tex.) treatment were assessed by various measures. Infected animals treated with orally administered lactoferrin showed reversals in all parameters. In addition, when recombinant human lactoferrin was used in combination with low doses of amoxicillin or tetracycline, there was an enhancement in gastritis-reducing activity. Possible mechanisms for these effects of lactoferrin are discussed. Lactoferrin has significant, orally active in vivo actions and should be further investigated for clinical situations involving Helicobacter infections where it may have utility when administered alone and also when given in combination with established antibiotic agents.Key words: lactoferrin, Helicobacter, gastritis, surface hydrophobicity.

scholarly journals In vitro and in vivo effect of human lactoferrin on glioblastoma growth

2015 ◽  
Vol 123 (4) ◽  
pp. 1026-1035 ◽  
Author(s):  
Antonietta Arcella ◽  
Maria Antonietta Oliva ◽  
Sabrina Staffieri ◽  
Silvia Aalberti ◽  
Giovanni Grillea ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Nude Mice ◽  
Tumor Size ◽  
Primary Cell ◽  
Continuous Cell Line ◽  
Human Lactoferrin ◽  
Primary Cell Lines

OBJECT Human lactoferrin (HLF) is a natural protein with antitumor activity. The aim of this study was to investigate the effects of HLF alone and in combination with temozolomide (TMZ), a conventional chemotherapeutic, on human glioblastoma (GBM) cells. METHODS The authors cultured fresh human primary cell lines NMD and FN and the continuous cell line U87MG to evaluate proliferation in the presence of HLF alone at different doses (1, 10, and 100 mg/ml, and 1 mg/ml) and in combination with TMZ. In in vivo experiments they assessed tumor size reduction in CD1 nude mice carrying an orthotopic GBM xenograft and orally treated with HLF. RESULTS Lactoferrin causes growth inhibition in the NMD and FN primary cell lines and in the U87MG continuous cell line. This inhibition seemed to be modulated by the downregulation of cyclin D1 and D4. Western blot and fluorescence-activated cell sorting analysis showed inhibition of the cell cycle in G0/G1 and G2 phases. When administered in nude mice, HLF (60 mg/kg/day) decreased tumor size about 30%, as shown in both histological analyses and high-field brain MRI. Administration of HLF with TMZ enhanced the effect of chemotherapy both in vitro and in vivo. CONCLUSIONS This study demonstrated that HLF can inhibit GBM cell growth, suggesting that this nontoxic substance may have a role in potentiating the effect of current TMZ treatment of GBM.

scholarly journals The ingestion and digestion of human lactoferrin by mouse peritoneal macrophages and the transfer of its iron into ferritin.

1977 ◽  
Vol 146 (3) ◽  
pp. 817-827 ◽  
Author(s):  
J L van Snick ◽  
B Markowetz ◽  
P L Masson
Keyword(s):  
Iron Content ◽  
Culture Medium ◽  
Reticuloendothelial System ◽  
Peritoneal Macrophages ◽  
Human Lactoferrin ◽  
Saturation Point ◽  
Intracellular Digestion ◽  
Additional Support ◽  
Mouse Peritoneal Macrophages

Human lactoferrin (Lf) labeled with 125I and/or 59Fe was found to be ingested in vitro by mouse peritoneal macrophages (MPM). The uptake measured after 15 h incubation reached a saturation point at a concentration of 200 microgram/ml in the culture medium, whatever was the iron content of Lf. In such conditions, the uptake of transferrin (Tf) used as a control was 10 times lower. At a concentration of 80 microgram/ml in the medium, one cell picked up about 0.7 X 10(6) molecules of Lf per hour, and 0.13 X 10(6) molecules of Tf per hour. Iron-saturated Lf disappeared from MPM with a half life of 14.5 h, whereas the halflife of iron-free Lf was 4.2 h. Concomitant with the intracellular digestion of Lf, the iron was transmitted to ferritin. These data provide additional support for the hypothesis that Lf plays a key role in iron turnover, especially at the level of the reticuloendothelial system where iron is recovered from the catabolism of erythrocytes.

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