scholarly journals Vibrio cholerae O1 outbreak isolates in Mozambique and South Africa in 1998 are multiple-drug resistant, contain the SXT element and the aadA2 gene located on class 1 integrons

2001 ◽  
Vol 48 (6) ◽  
pp. 827-838 ◽  
Author(s):  
A. Dalsgaard
Keyword(s):  
South Africa ◽  
Vibrio Cholerae ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Class 1 Integrons ◽  
Multiple Drug Resistant ◽  
Class 1 ◽  
Vibrio Cholerae O1

scholarly journals New Cluster of Plasmid-Located Class 1 Integrons in Vibrio cholerae O1 and a dfrA15 Cassette-Containing Integron in Vibrio parahaemolyticus Isolated in Angola

2006 ◽  
Vol 50 (7) ◽  
pp. 2493-2499 ◽  
Author(s):  
Daniela Ceccarelli ◽  
Anna Maria Salvia ◽  
Joana Sami ◽  
Piero Cappuccinelli ◽  
Mauro Maria Colombo
Keyword(s):  
Vibrio Cholerae ◽  
Resistance Genes ◽  
Vibrio Parahaemolyticus ◽  
Multiple Drug Resistance ◽  
Clinical Isolates ◽  
Conjugative Plasmid ◽  
Multiple Drug ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Vibrio Cholerae O1

ABSTRACT The resistance profile and its correlation with mobile genetic elements were investigated in 11 Vibrio cholerae O1 and 2 Vibrio parahaemolyticus clinical isolates, as well as in 1 V. cholerae O1 and 1 V. cholerae non-O1 environmental isolate, isolated between 1991 and 1996 in different provinces of Angola. All clinical isolates of V. cholerae O1 were resistant to ampicillin, chloramphenicol, trimethoprim, sulfamethoxazole, and tetracycline. They also contained a large conjugative plasmid (p3iANG) with a set of three class 1 integrons harboring dfrA15, blaP1, and qacH-aadA8 cassettes, which code for resistance to trimethoprim, beta-lactams, quaternary ammonium compounds, and aminoglycosides, clustered in a 19-kb region. Chloramphenicol (cat1), kanamycin (aph), sulfonamide (sul2), and tetracycline (tetG) resistance genes were also carried on the plasmid within the same 19-kb region. A chromosomal integron containing the dfrA15 cassette was also revealed in V. parahaemolyticus strains. SXT integrase genes were present in six V. cholerae isolates but apparently were not associated with known SXT-associated resistance genes. This study indicates that plasmids and integrons contributed mainly to the circulation of multiple-drug resistance determinants in Vibrio strains from Angola.

scholarly journals Drug susceptibility and its genetic basis in epidemic Vibrio cholerae O1 in Vietnam

2004 ◽  
Vol 132 (4) ◽  
pp. 595-600 ◽  
Author(s):  
M. EHARA ◽  
B. M. NGUYEN ◽  
D. T. NGUYEN ◽  
C. TOMA ◽  
N. HIGA ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Genetic Basis ◽  
Drug Susceptibility ◽  
Streptomycin Resistance ◽  
Class I ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Resistant Genes ◽  
Multiple Drug Resistant ◽  
Vibrio Cholerae O1

The drug susceptibility and genes responsible for the drug resistance of Vibrio cholerae O1 isolated in Vietnam in 1995, 2000 and 2002 were studied. The strains isolated in 1995 were resistant to streptomycin and harboured the class I integron which contained the aadA1 gene responsible for streptomycin resistance. The strains isolated in 2000 were devoid of a class I integron but were multiple-drug resistant and harboured SXT constin, with several drug-resistant genes. The genes responsible for streptomycin resistance were strA and strB. The strains isolated in 2002 were sensitive to all drugs examined, and the organisms were devoid of both class I integron and SXT constin. Cholera outbreaks in the three periods examined (1995, 2000 and 2002) were apparently due to different categories of V. cholerae O1.

Emergence of multiple drug-resistant Vibrio cholerae 01 in San Pedro Sula, Honduras

The Lancet ◽  
1997 ◽  
Vol 349 (9056) ◽  
pp. 924 ◽  
Author(s):  
Carol J Palmer ◽  
Jose M Dubon ◽  
Arba L Ager ◽  
Gail Shor-Posner ◽  
Marianna K Baum
Keyword(s):  
Vibrio Cholerae ◽  
Multiple Drug ◽  
Drug Resistant ◽  
San Pedro ◽  
Multiple Drug Resistant ◽  
San Pedro Sula

scholarly journals Whole genome analysis of ExPEC ST73 from a single hospital over a 2-year period identified different circulating clonal groups

2018 ◽  
Author(s):  
DR Bogema ◽  
J McKinnon ◽  
M Liu ◽  
N Hitchick ◽  
N Miller ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Mobile Genetic Elements ◽  
Bootstrap Support ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Genetic Elements ◽  
Multiple Drug Resistant ◽  
Class 1 ◽  
Tract Infections ◽  
Sydney Australia

AbstractST73 has emerged as one of the most frequently isolated extraintestinal pathogenic E. coli (ExPEC). To examine the localised diversity of ST73 clonal groups including their mobile genetic elements profile, we sequenced the genomes of 16 multiple drug-resistant ST73 isolates from patients with urinary tract infection from a single hospital in Sydney, Australia between 2009 and 2011. Genome sequences were used to generate a SNP-based phylogenetic tree to determine the relationship of these isolates in a global context with ST73 sequences (n=210) from public databases. There was no evidence of a dominant outbreak strain of ST73 in patients from this hospital, rather we identified at least eight separate groups, several of which reoccur, over a two-year period. The inferred phylogeny of all ST73 strains (n=226) including the ST73 Clone D i2 reference genome shows high bootstrap support and clusters into four major groups which correlate with serotype. The Sydney ST73 strains carry a wide variety of virulence-associated genes but the presence of iss, pic and several iron acquisition operons was notable.ImpactST73 is a major clonal lineage of ExPEC that causes urinary tract infections often with uroseptic sequelae but has not garnered substantial scientific interest as the globally disseminated ST131. Isolation of multiple antimicrobial resistant variants of ExPEC ST73 have increased in frequency, but little is known about the carriage of class 1 integrons in this sequence type and the plasmids that are likely to mobilise them. This pilot study examines the ST73 isolates within a single hospital in Sydney Australia and provides the first large-scale core-genome phylogenetic analysis of ST73 utilizing public sequence read datasets. We used this analysis to identify at least 8 sub-groups of ST73 within this single hospital. Mobile genetic elements associated with antibiotic resistance were less diverse and only three class 1 integron structures were identified, all sharing the same basic structure suggesting that the acquisition of drug resistance is a recent event. Genomic epidemiological studies are needed to further characterise established and emerging clonal populations of multiple drug resistant ExPEC to identify sources and aid outbreak investigations.

Vibrio cholerae non-O1, non-O139 strains isolated before 1992 from Varanasi, India are multiple drug resistant, contain intSXT, dfr18 and aadA5 genes

2008 ◽  
Vol 10 (4) ◽  
pp. 866-873 ◽  
Author(s):  
Harapriya Mohapatra ◽  
Saswat S. Mohapatra ◽  
Chinmay K. Mantri ◽  
Rita R. Colwell ◽  
Durg V. Singh
Keyword(s):  
Vibrio Cholerae ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Multiple Drug Resistant
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