Automated High Pressure Liquid Chromatographic System for Determination of Mannitol, Sorbitol, and Xylitol in Chewing Gums and Confections

1982 ◽  
Vol 65 (1) ◽  
pp. 76-78
Author(s):  
Eugene C Samarco ◽  
Ernest S Parente
Keyword(s):  
High Pressure ◽  
Standard Addition ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatograph ◽  
Liquid Chromatographic Method ◽  
Chewing Gums ◽  
Confectionery Products ◽  
Cation Exchange Column ◽  
Liquid Chromatographic

Abstract A rapid, precise, and reproducible automated high pressure liquid chromatographic method was developed to determine xylitol in the presence of mannitol and sorbitol in chewing gums and confectionery products. A mobile phase of water and methanol elutes the polyols simultaneously from a cation-exchange column without pretreatment or derivatization. Injections into the liquid chromatograph were made by an autosampler, and data reduction was performed with a programmable electronic integrator. Average recoveries for one level each of mannitol, sorbitol, and xylitol in 6 replicate samples prepared by the standard addition technique were 102.5,100.5, and 100.7%, respectively.

High Pressure Liquid Chromatographic Determination of Mannitol and Sorbitol in Sugarless Chewing Gums

1977 ◽  
Vol 60 (6) ◽  
pp. 1318-1320
Author(s):  
Eugene C Samarco
Keyword(s):  
High Pressure ◽  
Membrane Filter ◽  
Cation Exchange Resin ◽  
Chromatographic Determination ◽  
Phase System ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatograph ◽  
Chewing Gums ◽  
Liquid Chromatographic

Abstract A precise and accurate high pressure liquid chromatographic method is presented for the simultaneous separation and determination of mannitol and sorbitol in sugarless chewing gums. The 2 isomers are extracted into water, using a 2-phase system to facilitate dissolution of the chewing gum. The aqueous phase is filtered through a 0.45 μm pore membrane filter and injected into the liquid chromatograph. The mannitol and sorbitol are eluted from an Aminex Q-15S cation exchange resin with water as the mobile phase and are quantitated by using a differential refractive index detector. Average recoveries for mannitol and sorbitol are 98.9 and 99.2%, respectively.

Ion Pairing High Pressure Liquid Chromatographic Determination of Amaranth in Licorice Products

1981 ◽  
Vol 64 (6) ◽  
pp. 1411-1413
Author(s):  
William J Hurst ◽  
James M Mckim ◽  
Robert A Martin
Keyword(s):  
High Pressure ◽  
Ion Pairing ◽  
Chromatographic Determination ◽  
Buffer Solution ◽  
High Pressure Liquid Chromatographic ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A high pressure liquid chromatographic method is described for the determination of amaranth (FD&C Red No. 2; Red No. 2) in licorice products. The Red No. 2 is extracted with a basic buffer solution, cleaned up on a Sep-Pak column, chromatographed on a reverse phase column in the ion pairing mode, and detected at 254 nm. The procedure is time-conservative with accurate and precise results. Recovery data ranged from 93 to 104%, and coefficients of variation were less than 4% for standards and samples.

Determination of Uncombined Intermediates in FD&C Yellow No. 6 by High-Pressure Liquid Chromatography

1974 ◽  
Vol 57 (2) ◽  
pp. 358-359
Author(s):  
Manjeet Singh
Keyword(s):  
High Pressure ◽  
Chromatographic Method ◽  
Sulfanilic Acid ◽  
Sunset Yellow ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Two Samples ◽  
Liquid Chromatographic ◽  
Good Agreement

Abstract A high-pressure liquid chromatographic method is presented for the isolation and determination of uncombined intermediates in FD&C Yellow No. 6 (sunset yellow FCF, CI. No. 15895). Samples of FD&C Yellow No. 6 containing 0.1-0.4% sulfanilic acid, 0.1-0.4% 6-hydroxy-2-naphthalenesulfonic acid, and 0.3— 1.0% 6,6'-oxybis(2-naphthalenesulfonic acid) were prepared and analyzed using this method. Recoveries ranged between 94 and 101%. Twenty-two samples of FD&C Yellow No. 6 were analyzed by the conventional column elution chromatographic method as well as by the high-pressure liquid chromatographic method. Good agreement was obtained between the 2 methods.

High-Pressure Liquid Chromatographic Method for the Determination of Patulin in Apple Butter

1975 ◽  
Vol 58 (4) ◽  
pp. 754-756 ◽  
Author(s):  
George M Ware
Keyword(s):  
High Pressure ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Chromatographic Method ◽  
High Pressure Liquid Chromatographic ◽  
Ultraviolet Detector ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract Patulin is extracted from apple butter samples with ethyl acetate and the extract is cleaned up on a silica gel column, using benzene-ethyl acetate (75+25) as the eluant. High-pressure liquid chromatography, using a 25 cm Zorbax-Sil column, isooctane-ethyl ether-acetic acid (750+250+0.5) as the mobile solvent, and a 254 nm ultraviolet detector, is used for the determinative step. Under these conditions, patulin is eluted before 5-hydroxymethylfurfural, a component of apple butter which interferes with other liquid chromatographic and thin layer chromatographic methods. Recoveries of patulin added at levels of 34.6, 138.4, and 276.8 μg/kg ranged from 89.0 to 112.1%.

High Pressure Liquid Chromatographic Method for Probenecid in Flavored Oral Suspensions Containing Ampicillin: Collaborative Studies

1978 ◽  
Vol 61 (3) ◽  
pp. 687-694
Author(s):  
Paul J Vollmer ◽  
Thomas G Alexander ◽  
Carol Haneke
Keyword(s):  
High Pressure ◽  
Linear Response ◽  
Chromatographic Method ◽  
Random Order ◽  
High Pressure Liquid Chromatographic ◽  
Collaborative Studies ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A high pressure liquid chromatographic method for determining probenecid in oral suspensions of amoxicillin was applied to the determination of probenecid in oral suspensions of ampicillin. Three preparations containing various known amounts of probenecid in synthetic mixtures of ampicillin oral suspensions were analyzed by 5 chemists in an intralaborastory study, with satisfactory results. Blind duplicates of 3 prepared oral suspensions were sent to 12 collaborators, who were instructed to analyze the samples in a fixed random order. The standards showed a satisfactory linear response. Average recoveries of probenecid in the interlaboratory study for the 6 mixtures ranged from 95.2 to 99.1%, and the coefficients of variation ranged from 1.63 to 4.9%.

High Pressure Liquid Chromatographic Determination of Sulfanitran and Dinsed in Medicated Feeds and Premixes

1977 ◽  
Vol 60 (5) ◽  
pp. 1064-1066
Author(s):  
Kathleen L Eaves ◽  
Billy M Colvin ◽  
Alan R Hanks ◽  
Rodney J Bushway
Keyword(s):  
High Pressure ◽  
Colorimetric Method ◽  
Chromatographic Determination ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatograph ◽  
Ultraviolet Detector ◽  
Liquid Chromatographic Method ◽  
Medium Porosity ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A simple reverse phase high pressure liquid chromatographic method is described for determining sulfanitran (acetyl-p-nitrophenylsulfanilamide) and Dinsed (dinitrodiphenylsulfonylethylenediamine) in a variety of feed premixes and formulations. Feed premixes are extracted with dimethylformamide, and formulated feeds are extracted with hot methanol. The extract is filtered through medium porosity paper and injected into a liquid chromatograph equipped with a 254 nm ultraviolet detector and a 30 cm column packed with μBondapak C18. The mobile phase is acetonitrile-water (45+55) at a flow rate of 1.0 ml/min. Chromatography was complete in 10 min and peak heights were used for quantitation. Comparison of analyses of commercial samples by this method and by the AOAC colorimetric method, 42.176–42.179, showed close agreement. Recovery of spiked feed samples ranged from 98 to 105%. Butynorate and roxarsone, 2 other drugs which are normally found in combination with sulfanitran and Dinsed, do not interfere.

Quantitation of Radio-Labelled Vitamin K1 and Vitamin K1 Epoxide in Plasma by High Pressure Liquid Chromatography

1978 ◽  
Vol 39 (02) ◽  
pp. 466-473 ◽  
Author(s):  
Thorir D Bjornsson ◽  
Sarah E Swezey ◽  
Peter J Meffin ◽  
Terrence F Blaschke
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Coefficient Of Variation ◽  
Chromatographic Method ◽  
Vitamin K1 ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Wide Range ◽  
Liquid Chromatographic

SummaryA convenient, accurate and reproducible high pressure liquid chromatographic method for the quantitation of radio-labelled vitamin K1 and vitamin K1 epoxide in plasma is described. The method involves the determination of total ether extractable radioactivity, and a chromatographic separation to determine the relative quantities of radio-labelled vitamin K1 and vitamin K1 epoxide. The method is useful over a wide range of ratios of the two compounds, and has a coefficient of variation of approximately 5%.

Determination of acetaminophen and phenacetin in plasma by high-pressure liquid chromatography.

1977 ◽  
Vol 23 (6) ◽  
pp. 957-959 ◽  
Author(s):  
G R Gotelli ◽  
P M Kabra ◽  
L J Marton
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Chromatographic Method ◽  
Plasma Sample ◽  
Internal Standard ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Toxic Concentrations ◽  
Liquid Chromatographic

Abstract We describe a sensitive and precise high-pressure liquid chromatographic method in which acetoacetanilide is used as the internal standard to simultaneously determine acetaminophen and phenacetin in plasma. Therapeutic as well as toxic concentrations can be determined on as little as 0.1 ml of plasma. Sample preparation is rapid and chromatography is complete in 5 min. Quantitation is accurate at 0.5 mg/liter concentration for both drugs. Day-to-day precision within 5% is attainable. Of 36 other drugs tested, only theophylline interfered, with the determination of acetaminophen.

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