Determination of acetaminophen and phenacetin in plasma by high-pressure liquid chromatography.

1977 ◽  
Vol 23 (6) ◽  
pp. 957-959 ◽  
Author(s):  
G R Gotelli ◽  
P M Kabra ◽  
L J Marton
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Chromatographic Method ◽  
Plasma Sample ◽  
Internal Standard ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Toxic Concentrations ◽  
Liquid Chromatographic

Abstract We describe a sensitive and precise high-pressure liquid chromatographic method in which acetoacetanilide is used as the internal standard to simultaneously determine acetaminophen and phenacetin in plasma. Therapeutic as well as toxic concentrations can be determined on as little as 0.1 ml of plasma. Sample preparation is rapid and chromatography is complete in 5 min. Quantitation is accurate at 0.5 mg/liter concentration for both drugs. Day-to-day precision within 5% is attainable. Of 36 other drugs tested, only theophylline interfered, with the determination of acetaminophen.

High-Pressure Liquid Chromatographic Method for the Determination of Patulin in Apple Butter

1975 ◽  
Vol 58 (4) ◽  
pp. 754-756 ◽  
Author(s):  
George M Ware
Keyword(s):  
High Pressure ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Chromatographic Method ◽  
High Pressure Liquid Chromatographic ◽  
Ultraviolet Detector ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract Patulin is extracted from apple butter samples with ethyl acetate and the extract is cleaned up on a silica gel column, using benzene-ethyl acetate (75+25) as the eluant. High-pressure liquid chromatography, using a 25 cm Zorbax-Sil column, isooctane-ethyl ether-acetic acid (750+250+0.5) as the mobile solvent, and a 254 nm ultraviolet detector, is used for the determinative step. Under these conditions, patulin is eluted before 5-hydroxymethylfurfural, a component of apple butter which interferes with other liquid chromatographic and thin layer chromatographic methods. Recoveries of patulin added at levels of 34.6, 138.4, and 276.8 μg/kg ranged from 89.0 to 112.1%.

Quantitation of Radio-Labelled Vitamin K1 and Vitamin K1 Epoxide in Plasma by High Pressure Liquid Chromatography

1978 ◽  
Vol 39 (02) ◽  
pp. 466-473 ◽  
Author(s):  
Thorir D Bjornsson ◽  
Sarah E Swezey ◽  
Peter J Meffin ◽  
Terrence F Blaschke
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Coefficient Of Variation ◽  
Chromatographic Method ◽  
Vitamin K1 ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Wide Range ◽  
Liquid Chromatographic

SummaryA convenient, accurate and reproducible high pressure liquid chromatographic method for the quantitation of radio-labelled vitamin K1 and vitamin K1 epoxide in plasma is described. The method involves the determination of total ether extractable radioactivity, and a chromatographic separation to determine the relative quantities of radio-labelled vitamin K1 and vitamin K1 epoxide. The method is useful over a wide range of ratios of the two compounds, and has a coefficient of variation of approximately 5%.

Fast gradient normal-phase high pressure liquid chromatography for rapid baseline separation of vitamin E forms in palm-derived tocotrienol rich fractions (TRFs)

2017 ◽  
Vol 9 (35) ◽  
pp. 5211-5218
Author(s):  
Oon Hock Tan ◽  
Emily Hui Peng Tan ◽  
Ing Hong Ooi
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Vitamin E ◽  
Chromatographic Method ◽  
Normal Phase ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Fast Gradient ◽  
Liquid Chromatographic

A normal phase high pressure liquid chromatographic method was developed for the simultaneous determination of nine vitamin E forms comprised of α-tocomonoenol, α-, β-, γ-, and δ-tocopherols and α-, β-, γ-, and δ-tocotrienols in tocotrienol rich fractions (TRFs) derived from palm oil.

Determination of Dexamethasone in Milk by High Pressure Liquid Chromatography

1977 ◽  
Vol 60 (1) ◽  
pp. 210-212 ◽  
Author(s):  
Anneli M DePaolis ◽  
Gabrielle Schnabel ◽  
S E Katz ◽  
Joseph D Rosen
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Chromatographic Method ◽  
High Pressure Liquid Chromatographic ◽  
Holstein Friesian ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Friesian Cows

Abstract A rapid and sensitive high pressure liquid chromatographic method for the analysis of dexamethasone (9α-fluoro-11β,17α,21-trihydroxy-16α-methylpregna-1,4-diene-3,20-dione) in milk has been developed. The corticosteroid can be quantitated at 20 ppb, with the limit of detectability estimated at 5 ppb. Recoveries ranged from 72 to 94%. After dexamethasone was injected into 5 Holstein-Friesian cows, no residues were found in milk, even after the first milking.

Determination of carbamazepine in blood or plasma by high-pressure liquid chromatography.

1976 ◽  
Vol 22 (7) ◽  
pp. 1070-1072 ◽  
Author(s):  
P M Kabra ◽  
L J Marton
Keyword(s):  
High Pressure ◽  
Chromatographic Method ◽  
Limit Of Detection ◽  
Internal Standard ◽  
High Pressure Liquid Chromatographic ◽  
Blood Constituents ◽  
Liquid Chromatographic Method ◽  
Gas Chromatographic Method ◽  
Liquid Chromatographic

Abstract We described a sensitive and precise high-pressure liquid-chromatographic method in which 5-(p-methylphenyl)-5-phenylhydantoin is used as the internal standard in determining carbamazepine in whole blood or plasma. Carbamazepine is well separated from normal blood constituents in less than 8 min, and other commonly used anticonvulsants do not interfere with the analysis. The sensitivity of this method is adequate to quantitate 0.25 mg of carbamazepine per liter in 2 ml of sample, and the lower limit of detection is 100 ng. Twenty specimens were analyzed by a gas-chromatographic method and by the present method; the resulting correlation coefficient was greater than .980.

High-Pressure Liquid Chromatography of 4,4′-Diazoaminobis-(5-methoxy-2-methylbenzenesulfonic Acid) in FD&C Red No. 40

1976 ◽  
Vol 59 (4) ◽  
pp. 838-845
Author(s):  
Daniel M Marmion
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Chromatographic Method ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Chromatographic Procedure ◽  
The Stability ◽  
Liquid Chromatographic

Abstract Ten analysts representing 7 laboratories participated in the evaluation of a high-pressure liquid chromatographic procedure for the determination of 4,4′-diazoaminobis(5-methoxy-2-methylbenzenesulfonic acid) (DMMA) in FD&C Red No. 40; each collaborator analyzed 7 samples. Between-laboratory standard deviations ranged from 0.0015 to 0.0033% for samples nominally containing 0–0.05% DMMA. The stability of DMMA vs. pH was also studied. It is recommended that the high-pressure liquid chromatographic method be further studied.

Determination of Uncombined Intermediates in FD&C Yellow No. 6 by High-Pressure Liquid Chromatography

1974 ◽  
Vol 57 (2) ◽  
pp. 358-359
Author(s):  
Manjeet Singh
Keyword(s):  
High Pressure ◽  
Chromatographic Method ◽  
Sulfanilic Acid ◽  
Sunset Yellow ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Two Samples ◽  
Liquid Chromatographic ◽  
Good Agreement

Abstract A high-pressure liquid chromatographic method is presented for the isolation and determination of uncombined intermediates in FD&C Yellow No. 6 (sunset yellow FCF, CI. No. 15895). Samples of FD&C Yellow No. 6 containing 0.1-0.4% sulfanilic acid, 0.1-0.4% 6-hydroxy-2-naphthalenesulfonic acid, and 0.3— 1.0% 6,6'-oxybis(2-naphthalenesulfonic acid) were prepared and analyzed using this method. Recoveries ranged between 94 and 101%. Twenty-two samples of FD&C Yellow No. 6 were analyzed by the conventional column elution chromatographic method as well as by the high-pressure liquid chromatographic method. Good agreement was obtained between the 2 methods.

High Pressure Liquid Chromatographic Method for Probenecid in Flavored Oral Suspensions Containing Ampicillin: Collaborative Studies

1978 ◽  
Vol 61 (3) ◽  
pp. 687-694
Author(s):  
Paul J Vollmer ◽  
Thomas G Alexander ◽  
Carol Haneke
Keyword(s):  
High Pressure ◽  
Linear Response ◽  
Chromatographic Method ◽  
Random Order ◽  
High Pressure Liquid Chromatographic ◽  
Collaborative Studies ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A high pressure liquid chromatographic method for determining probenecid in oral suspensions of amoxicillin was applied to the determination of probenecid in oral suspensions of ampicillin. Three preparations containing various known amounts of probenecid in synthetic mixtures of ampicillin oral suspensions were analyzed by 5 chemists in an intralaborastory study, with satisfactory results. Blind duplicates of 3 prepared oral suspensions were sent to 12 collaborators, who were instructed to analyze the samples in a fixed random order. The standards showed a satisfactory linear response. Average recoveries of probenecid in the interlaboratory study for the 6 mixtures ranged from 95.2 to 99.1%, and the coefficients of variation ranged from 1.63 to 4.9%.

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