Stored Wheat Insect Infestation Related to Uric Acid as Determined by Liquid Chromatography

1984 ◽  
Vol 67 (3) ◽  
pp. 644-647
Author(s):  
Randy L Wehling ◽  
David L Wetzel ◽  
John R Pedersen
Keyword(s):  
Uric Acid ◽  
Liquid Chromatography ◽  
Acid Content ◽  
Sitophilus Oryzae ◽  
Correlation Coefficients ◽  
Rice Weevil ◽  
Growth Stages ◽  
Granary Weevil ◽  
Lesser Grain Borer ◽  
Late Instar

Abstract Liquid chromatography is used to measure the uric acid content of wheat internally infested by various growth stages of granary weevil (Sitophilus granarius), rice weevil (Sitophilus oryzae), and lesser grain borer (Ryzopertha dominica). Good correlation exists between numbers of insects and uric acid content of grain infested by a given stage of an internally infesting stored product insect, with correlation coefficients ranging from 0.970 to 0.998. Uric acid content of infested grain increases as the insect life cycle progresses, with granary weevil producing the greatest quantities of uric acid followed in order by rice weevil and lesser grain borer. A detection limit for the analytical procedure of less than 1.0 ppm uric acid allows detection of infestation levels as low as one kernel per 100 g grain for late instar granary weevil larvae. This limit is sufficiently sensitive to detect levels of infestation that may be commonly encountered in commerce.

Evaluation of wheat landraces of north-western Himalaya against rice weevil, Sitophilus oryzae L. vis-à-vis physical seed parameters

2016 ◽  
Vol 15 (4) ◽  
pp. 321-326 ◽  
Author(s):  
Kuldeep Tripathi ◽  
S. K. Chauhan ◽  
P. G. Gore ◽  
P. S. Mehta ◽  
I. S. Bisht ◽  
...  
Keyword(s):  
Sitophilus Oryzae ◽  
Correlation Coefficients ◽  
Growth Index ◽  
Western Himalaya ◽  
Test Weight ◽  
Rice Weevil ◽  
Physical Parameters ◽  
Length Width ◽  
Wheat Landraces ◽  
North Western

AbstractThe north-western Himalaya is one of the rich repositories of wheat genetic resources because of the preponderance of locally developed traditional crop varieties owing to high agro-climatic heterogeneity and local socio-cultural diversity. In the present study, 100 wheat landraces of this diversity rich region were evaluated for variability in physical parameters of seed to understand the basis of resistance against rice weevil, Sitophilus oryzae. The evaluation was based on the parameter of growth index (GI) of S. oryzae in different landraces. GI was correlated with different quantitative physical seed parameters, viz. hardness, length, width, length × width, test weight and qualitative parameter seed colour were studied to work out if these were related to resistance/susceptibility. Based on the parameter of GI, the six landraces viz. IC266831, IC266872, IC393109, IC392578, IC444217 and IC589276 were identified as resistant. Correlation coefficients between GI of S. oryzae and physical parameters of wheat landraces indicated that GI had significant positive relation with length × width (r = +0.573) and test weight (r = +0.549) indicated that small seeds confer resistance to S. oryzae. Also significant negative relation (r = −0.457) with GI of S. oryzae and seed hardness, indicated that hard seeds were relatively more resistant to S. oryzae.

Susceptibility of Twelve Genotypes of Triticale to the Rice Weevil (Coleoptera: Curculionidae) and the Lesser Grain Borer (Coleoptera: Bostrichidae)2

1991 ◽  
Vol 26 (3) ◽  
pp. 339-344 ◽  
Author(s):  
J. E. Baker ◽  
F. H. Arthur ◽  
P. L. Bruckner
Keyword(s):  
Moisture Content ◽  
Growth Parameters ◽  
Oryza Sativa L ◽  
Sitophilus Oryzae ◽  
Brown Rice ◽  
Rice Weevil ◽  
Rhyzopertha Dominica ◽  
Progeny Production ◽  
Lesser Grain Borer ◽  
Total Progeny

Population development by the rice weevil, Sitophilus oryzae (L.) and feeding damage caused by the lesser grain borer, Rhyzopertha dominica (F.), were assessed on twelve triticale (X Triticosecale Wittmack) genotypes maintained at 25°C and at 11.7 or 14.2% moisture content. Among genotypes at 14.2% moisture content, total progeny production by the rice weevil ranged from 7.1 (GA82014) to 8.8 (87AB13541) weevils per female-day; mean time to adult emergence ranged from 35.5 (GA82014) to 36.9 days (87AB13541 and CT4699); and rate of emergence (a measure of the slope of the cumulative emergence curve at the average emergence day) ranged from 0.59 (87AB 13541) to 0.72 (‘Morrison’). Although there were some statistical differences among these population growth parameters on different triticale genotypes, all genotypes were at least as susceptible as the long grain brown rice (Oryza sativa L.) and soft red winter wheat (Triticum aestivum L., cultivar ‘Florida 302’) controls tested concurrently. Development time of rice weevils was significantly delayed and total progeny production after 65 days was reduced about 17-fold on triticale genotypes at 11.7% moisture content. About 3-fold more frass was produced by lesser grain borers feeding for 7 days at 25°C on triticale genotypes at 14.2% moisture content compared to that obtained on genotypes at 11.7% moisture content. Significantly more feeding and frass production by lesser grain borers occurred on triticale and wheat than occurred on rice.

scholarly journals Deterioration of Grain Quality of Wheat by Rice Weevil, Sitophilus oryzae (L.) during Storage

2021 ◽  
Author(s):  
Subash Singh ◽  
D.K. Sharma
Keyword(s):  
Durum Wheat ◽  
Sitophilus Oryzae ◽  
Biochemical Properties ◽  
Rice Weevil ◽  
Rhyzopertha Dominica ◽  
Wheat Cultivars ◽  
Storage Pests ◽  
Granary Weevil ◽  
Stored Wheat ◽  
Grain Damage

Background: A number of storage pests viz., Rhyzopertha dominica (F.), rice weevil, Sitophilus oryzae (L.), granary weevil, Sitophilus granarius (L.) and Khapra beetle, Trogoderma granarium (Evert.) damage stored wheat. However, S. oryzae is considered a primary pest of stored wheat and has been reported to prefer soft textured wheat cultivars.Methods: The grains of three bread (soft textured) viz., HD2967, PDW314, PBW658 and three durum wheat (hard textured) cultivars viz., WHD943, PBW621, PDW291 were screened for feeding response by rice weevil, Sitophilus oryzae L. in the laboratory. Each cultivar seed was properly cleaned and disinfested before its use. A 100g seed sample of each cultivar was taken into the plastic jars. Of the pure culture, ten 1-2 week young one insects (1:1 sex ratio) were released into the jars each containing different cultivar grains. The jars were covered with white muslin as three separate storage sets, i.e., 30, 60 and 90 days after storage to record data observations. Result: Durum wheat cultivars being low in protein contents were comparatively less preferred for pest feeding and cultivar PDW291 was found highly resistant based on low insect emergence, grain damage and weight loss. The bread wheat cultivars due to high protein contents were highly preferred by the pest and cultivar HD2967 showed maximum preference. The biochemical properties like ash contents and crude fibres showed positive while crude fats and protein a negative correlation with the pest infestation. 

scholarly journals An influence of herbs on the sex structure in populations of insect pests of stored products

2012 ◽  
Vol 52 (4) ◽  
pp. 463-466
Author(s):  
Małgorzata Kłyś
Keyword(s):  
Insect Pests ◽  
Sitophilus Oryzae ◽  
Rice Weevil ◽  
Insect Species ◽  
Rhyzopertha Dominica ◽  
Mentha Piperita ◽  
Lesser Grain Borer ◽  
Sex Structure ◽  
Males And Females ◽  
Lavandula Officinalis

Abstract The effects of powdered herbs (lavender Lavandula officinalis L., peppermint Mentha piperita L. and basil Ocimium basilicum L.) added to the food substances of three insect species: the rice weevil Sitophilus oryzae (L.), lesser grain borer Rhyzopertha dominica (F.), and the saw-toothed grain beetle Oryzaephilus surinamensis (L.) on the life span and development of the three insect species as well as habitat conditions, sex structure, and the survivability of males and females were considered. The experiments were conducted in the laboratory at a temperature of 28°C and relative humidity (RH) 60 ±5%. In all the studied populations, high female mortality was found (♂/♀ < 1). The studied populations were: the rice weevil and the lesser grain borer cultured in wheat grain with herb additions, and the saw-toothed grain beetle cultured in semolina with herb additions. Among the herbs used, peppermint caused a significant effect in the form of increased mortality rates among females of S. oryzae and O. surinamensis. Lavender and basil had a similar impact among R. dominica females. The herbs applied in the experiments intensified the mortality of females and, consequently, led to the disappearance of entire populations.

Deproteinizing methods evaluated for determination of uric acid in serum by reversed-phase liquid chromatography with ultraviolet detection.

1987 ◽  
Vol 33 (8) ◽  
pp. 1427-1430 ◽  
Author(s):  
R Sakuma ◽  
T Nishina ◽  
M Kitamura
Keyword(s):  
Uric Acid ◽  
Liquid Chromatography ◽  
Perchloric Acid ◽  
High Performance ◽  
Reversed Phase ◽  
Precipitation Method ◽  
Zinc Hydroxide ◽  
Good Precision ◽  
Ultraviolet Detection

Abstract We evaluated six deproteinizing methods for determination of uric acid in serum by "high-performance" liquid chromatography with ultraviolet detection: those involving zinc hydroxide, sodium tungstate, trichloroacetic acid, perchloric acid, acetonitrile, and centrifugal ultrafiltration (with Amicon MPS-1 devices). We used a Toyosoda ODS-120A reversed-phase column. The mobile phase was sodium phosphate buffer (40 mmol/L, pH 2.2) containing 20 mL of methanol per liter. Absorbance of the eluate was monitored at 284 nm. The precipitation method with perchloric acid gave high recoveries of uric acid and good precision, and results agreed with those by the uricase-catalase method of Kageyama (Clin Chim Acta 1971;31:421-6).

scholarly journals THE URIC ACID CONTENT OF THE BLOOD OF NEW-BORNS

1917 ◽  
Vol 31 (1) ◽  
pp. 261-268
Author(s):  
F.B. Kingsbury ◽  
J.P. Sedgwick
Keyword(s):  
Uric Acid ◽  
Acid Content
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