Ochratoxin A in Cow’s Milk and in Human Milk with Corresponding Human Blood Samples

Abstract A method for determining ochratoxin A in milk has been elaborated in which the sample was subjected to a liquid-liquid extraction step and then purified on a silica gel column packed in a Pasteur pipet. The purified samples were analyzed by ion-pair liquid chromatography with fluorescence detection. The detection and quantitation limits for determination of ochratoxin A in cow’s milk were 10 and 40 ng ochratoxin A/L milk, respectively. The same limits were valid for the analysis of human milk. A total of 36 cow’s milk and 40 human milk samples were analyzed. All samples were collected in Sweden. Ochratoxin A was found in 5 (14%) of the cow’s milk samples (range 10-40 ng/mL) and in 23 (58%) of the human milk samples (range 10-40 ng/L). Blood samples were collected from the mothers who gave milk samples. A total of 39 samples were analyzed. All blood samples contained ochratoxin A in concentrations exceeding the quantitation limit (60 ng/L blood). The mean concentration of ochratoxin A in the samples was 167 ng/L blood (range 90-940 ng/L). The concentration of ochratoxin A in human milk was ≤0.1 of that in the human blood.

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Human Breast Milk Contains Bovine lgG. Relationship to Infant Colic?

PEDIATRICS ◽

10.1542/peds.87.4.439 ◽

1991 ◽

Vol 87 (4) ◽

pp. 439-444

Author(s):

Patrick S. Clyne ◽

Anthony Kulczycki

Keyword(s):

Breast Milk ◽

Human Milk ◽

Milk Protein ◽

Cow’S Milk ◽

Human Breast Milk ◽

Human Breast ◽

Cow's Milk ◽

Milk Samples ◽

Infant Colic ◽

Β Lactoglobulin

Previous studies have suggested that an unidentified cow's milk protein, other than β-lactoglobulin and casein, might play a pathogenetic role in infant colic. Therefore, a radioimmunoassay was used to analyze human breast milk and infant formula samples for the presence of bovine IgG. Milk samples from 88 of the 97 mothers tested contained greater than 0.1 µg/mL of bovine IgG. In a study group of 59 mothers with infants in the colic-prone 2- to 17-week age group, the 29 mothers of colicky infants had higher levels of bovine IgG in their breast milk (median 0.42 µg/mL) than the 30 mothers of noncolicky infants (median 0.32 µg/mL) (P < .02). The highest concentrations of bovine IgG observed in human milk were 8.5 and 8.2 µg/mL. Most cow's milk-based infant formulas contained 0.6 to 6.4 µg/mL of bovine IgG, a concentration comparable with levels found in many human milk samples. The results suggest that appreciable quantities of bovine IgG are commonly present in human milk, that significantly higher levels are present in milk from mothers of colicky infants, and that bovine IgG may possibly be involved in the pathogenesis of infant colic.

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Evaluation of role of biomakers on diabetes mellitus by using human blood samples

International Journal of Pharma and Bio Sciences ◽

10.22376/ijpbs.2019.10.2.p86-95 ◽

2019 ◽

Vol 10 (2) ◽

Author(s):

G. NAGARAJA PERUMAL ◽

MOHAN S.

Keyword(s):

Diabetes Mellitus ◽

Human Blood ◽

Blood Samples ◽

Human Blood Samples

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ICP-MS Analysis of Trace Element Concentrations in Cow’s Milk Samples from Supermarkets in Istanbul, Turkey

Biological Trace Element Research ◽

10.1007/s12011-019-01708-4 ◽

2019 ◽

Vol 193 (1) ◽

pp. 166-173 ◽

Cited By ~ 1

Author(s):

Devrim Saribal

Keyword(s):

Trace Element ◽

Cow’S Milk ◽

Cow's Milk ◽

Milk Samples ◽

Element Concentrations ◽

Ms Analysis ◽

Icp Ms

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A frogspawn-like Ag@C core–shell structure for an ultrasensitive label-free electrochemical immunosensing of carcinoembryonic antigen in blood plasma

RSC Advances ◽

10.1039/d1ra00910a ◽

2021 ◽

Vol 11 (27) ◽

pp. 16339-16350

Author(s):

Mengkui Ding ◽

Ling Zha ◽

Hui Wang ◽

Jinyao Liu ◽

Peiwu Chen ◽

...

Keyword(s):

Blood Plasma ◽

Carcinoembryonic Antigen ◽

Human Blood ◽

Shell Structure ◽

Core Shell ◽

Label Free ◽

Blood Samples ◽

Human Blood Samples ◽

Core Shell Structure ◽

Electrochemical Immunosensing

Novel frogspawn-like Ag@C nanoparticles were successfully used to fabricate an ultrasensitive electrochemical immunosensing platform toward CEA in human blood samples.

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Borrelia burgdorferi Survival in Human Blood Samples

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.1988.tb31899.x ◽

1988 ◽

Vol 539 (1 Lyme Disease) ◽

pp. 444-445 ◽

Cited By ~ 8

Author(s):

GUY BARANTON ◽

ISABELLE SAINT-GIRONS

Keyword(s):

Borrelia Burgdorferi ◽

Human Blood ◽

Blood Samples ◽

Human Blood Samples

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Gastroduodenal Motility in Neonates: Response to Human Milk Compared With Cow's Milk Formula

PEDIATRICS ◽

10.1542/peds.80.3.434 ◽

1987 ◽

Vol 80 (3) ◽

pp. 434-438

Author(s):

T. Tomomasa ◽

P. E. Hyman ◽

K. Itoh ◽

J. Y. Hsu ◽

T. Koizumi ◽

...

Keyword(s):

Breast Milk ◽

Human Milk ◽

Infant Formula ◽

High Amplitude ◽

Cow’S Milk ◽

Milk Formula ◽

Wave Form ◽

Fasting State ◽

Cow's Milk ◽

Gastroduodenal Motility

It is known that breast milk empties more quickly from the stomach than does infant formula. We studied the difference in gastroduodenal motility between neonates fed with human milk and those fed with infant formula. Twenty-four five-to 36-day-old neonates were fed with mother's breast milk or with a cow's milk-based formula. Postprandlial gastroduodenal contractions were recorded manometrically for three hours. Repetitive, high-amplitude nonmigrating contractions were the dominant wave form during the postprandial period. The number of episodes, duration, amplitude, and frequency of nonmigrating contractions were not different following the different feedings. The migrating myoelectric complex, which signals a return to the interdigestive (fasting) state, appeared in 75% of breast milk-fed infants but only 17% of formula-fed infants (P < .05) within the three-hour recording period. Because contractions were similar following the two meals, but a fasting state recurred more rapidly in breast-fed infants, we conclude that factors other than phasic, nonpropagated antroduodenal contractions were responsible for the differences in gastric emptying between breast milk and formula.

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