scholarly journals Supplementing organic-complexed or inorganic Co, Cu, Mn, and Zn to beef cows during gestation: physiological and productive response of cows and their offspring until weaning

2021 ◽  
Author(s):  
Kelsey M Harvey ◽  
Reinaldo F Cooke ◽  
Eduardo A Colombo ◽  
Bruna Rett ◽  
Osvaldo A de Sousa ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Milk Production ◽  
Treatment Effects ◽  
Muscle Development ◽  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Beef Cows ◽  
Condition Score ◽  
To Receive

Abstract One hundred and ninety non-lactating, pregnant beef cows (¾ Bos taurus and ¼ Bos indicus; 138 multiparous and 52 primiparous) were assigned to this experiment at 117 ± 2.2 d of gestation (d 0). Cows were ranked by parity, pregnancy type (artificial insemination = 102, natural service = 88), body weight (BW) and body condition score (BCS), and assigned to receive a supplement containing: 1) sulfate sources of Cu, Co, Mn, and Zn (INR; n = 95) or 2) an organic complexed source of Cu, Mn, Co, and Zn (AAC; Availa®4; Zinpro Corporation, Eden Prairie, MN; n = 95). The INR and AAC provided the same daily amount of Cu, Co, Mn, and Zn, based on 7 g of the AAC source. From d 0 to calving, cows were maintained in a single pasture and were segregated three times weekly into 1 of 24 individual feeding pens to receive treatments. Cow BW and BCS were recorded on d -30, d 97, upon calving, and at weaning (d 367). Milk production was estimated at 42 ± 0.5 d postpartum via weigh-suckle-weigh (WSW). Liver biopsies were performed in 30 cows/treatment on d -30, 97, upon calving, and the day after WSW. Calf BW was recorded at birth and weaning. Liver and longissimus muscle (LM) biopsies were performed in 30 calves/treatment upon calving and 24 h later, the day after WSW, and at weaning. No treatment effects were detected (P ≥ 0.49) for cow BCS during gestation, despite AAC cows having greater (P = 0.04) BW on d 97. Liver Co concentrations were greater (P < 0.01) for AAC compared to INR cows, and liver concentrations of Cu were greater (P = 0.02) for INR compared to AAC cows on d 97. Upon calving, INR cows had greater (P ≤ 0.01) liver Cu and Zn concentrations compared to AAC cows. No other treatment differences were noted (P ≥ 0.17) for cow and calf liver trace mineral concentrations. Cows receiving AAC had greater (P = 0.04) hepatic mRNA expression of metallothionein 1A at calving, and their calves had greater (P = 0.04) hepatic mRNA expression of superoxide dismutase at weaning. Milk production did not differ between AAC and INR cows (P = 0.70). No treatment effects were detected (P ≥ 0.29) for mRNA expression of LM genes associated with adipogenic or muscle development activities in calves at birth and weaning. Calf birth and weaning BW also did not differ (P ≥ 0.19) between treatments. In summary, supplementing Co, Cu, Zn, and Mn as organic complexed or sulfate sources to beef cows during the last 5 mo of gestation yielded similar cow-calf productive responses until weaning.

scholarly journals 166 EFFECT OF TREATMENT WITH hCG OR GnRH AT THE TIME OF EMBRYO TRANSFER ON PREGNANCY RATES IN COWS SYNCHRONIZED WITH PROGESTERONE VAGINAL DEVICES, ESTRADIOL BENZOATE, AND eCG

2005 ◽  
Vol 17 (2) ◽  
pp. 234 ◽  
Author(s):  
R. Tribulo ◽  
E. Balla ◽  
L. Cutaia ◽  
G.A. Bo ◽  
P.S. Baruselli ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Beef Cows ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Condition Score ◽  
Pregnancy Status ◽  
To Receive

Although several studies have investigated the relationship between circulating progesterone and pregnancy rates in cattle, the beneficial effect of treatments that increase progesterone concentrations, by insertion of a progesterone (P4) releasing device or induction of an accessory CL with hCG, GnRH, or LH treatment, has resulted in inconsistent effects on pregnancy rates in embryo recipients. An experiment was designed to evaluate the effect of hCG or GnRH treatment, given at the time of embryo transfer without estrus detection, on pregnancy rates in recipients treated with intrauterine P4-releasing devices, estradiol benzoate (EB), and eCG. The experiment was performed in two replicates; non-lactating Bos taurus × Bos indicus crossbred beef cows with a body condition score between 2.5 to 3.5 (1-to-5 scale) were used (replicate 1, n = 180; replicate 2, n = 140). All cows received 1 g of P4 via a P4-releasing device (DIB, Syntex, Argentina) and 2 mg EB i.m. (Syntex) on Day 0, and 400 IU of eCG i.m. (Novormon 5000, Syntex) plus 150 μg d(+)cloprostenol i.m. (Ciclase, Syntex) on Day 5. DIBs were removed on Day 8 and all cows received 1 mg EB i.m. on Day 9. Recipients were not observed for signs of estrus, and those >1 CL, or a single CL with an area >256 mm2, received 195 Grade 1 and 46 Grade 2 frozen/thawed “direct transfer” embryos on Day 17. At the time of embryo transfer, recipients were randomly allocated to 1 of 3 treatment groups to receive 1500 IU hCG (Ovusyn, Syntex), 50 μg Lecirelina (GnRH, Gonasyn, Syntex), or no treatment (control) at that time. Ovarian ultrasonography was performed on Day 0 to determine ovarian status (only cows with a CL or a follicle >10 mm and uterine tone were used), on Day 17 to measure CL area, and 40 days after embryo transfer to determine pregnancy status. Data were analyzed by logistic regression and the effects of replication, technician, treatment, and embryo quality were considered in the model. From the 320 recipients treated with a DIB plus EB and eCG, 241 (75.3%) were selected to receive an embryo. Nine (3.7%) and 1 (0.4%) of the selected recipients had 2 and 3 CL, respectively. Pregnancy rates did not differ between replicates (replicate 1: 80/140, 57.1%; and replicate 2: 57/101, 56.4%; P = 0.84), technicians (technician 1: 65/118, 55.1%; and technician 2: 72/123, 58.5%; P = 0.64), or treatments (hCG: 43/80, 53.8%; GnRH: 45/83, 54.2%; and control: 49/78, 62.8% P = 0.99). However, pregnancy rates were higher (P = 0.001) in recipients receiving Grade 1 embryos (121/195, 62.1%) than in those receiving Grade 2 embryos (16/46, 34.8%). GnRH or hCG treatment at the time of embryo transfer did not increase pregnancy rates in recipients synchronized with P4 releasing devices, EB, and eCG. Research was supported by Syntex S.A., Estancia El Mangrullo S.A., and Agencia Cordoba Ciencia S.E.

scholarly journals Supplementing organic-complexed or inorganic Co, Cu, Mn, and Zn to beef cows during gestation: post-weaning responses of offspring reared as replacement heifers or feeder cattle

2021 ◽  
Author(s):  
Kelsey M Harvey ◽  
Reinaldo F Cooke ◽  
Eduardo A Colombo ◽  
Bruna Rett ◽  
Osvaldo A de Sousa ◽  
...  
Keyword(s):  
Treatment Effects ◽  
Bos Taurus ◽  
Body Condition Score ◽  
Average Daily Gain ◽  
Bos Indicus ◽  
Beef Cows ◽  
Single Group ◽  
Female Progeny ◽  
Feeder Cattle ◽  
To Receive

Abstract One hundred and ninety non-lactating, pregnant beef cows (¾ Bos taurus and ¼ Bos indicus; 138 multiparous and 52 primiparous) were assigned to this experiment at 117 ± 2.2 d of gestation (d 0). Cows were ranked by parity, pregnancy type (artificial insemination = 102, natural service = 88), body weight (BW) and body condition score, and assigned to receive a supplement containing: 1) sulfate sources of Cu, Co, Mn, and Zn (INR; n = 95) or 2) an organic complexed source of Cu, Mn, Co, and Zn (AAC; Availa®4; Zinpro Corporation, Eden Prairie, MN; n = 95). The INR and AAC provided the same daily amount of Cu, Co, Mn, and Zn, based on 7 g of the AAC source. From d 0 to calving, cows were maintained in a single pasture and segregated three times weekly into 1 of 24 individual feeding pens to receive treatments. Calves were weaned on d 367 (200 ± 2 d of age), managed as a single group for a 45-d preconditioning period (d 367 to 412), and transferred to a single oat (Avena sativa L.) pasture on d 412. Heifer calves were moved to an adjacent oat pasture on d 437, where they remained until d 620. Heifer puberty status was verified weekly (d 437 to d 619) based on plasma progesterone concentrations. Steer calves were shipped to a commercial feedlot on d 493, where they were managed as a single group until slaughter (d 724). Plasma cortisol concentration was greater (P = 0.05) in AAC calves at weaning but tended to be less (P = 0.10) on d 370 compared with INR calves. Mean plasma haptoglobin concentration was greater (P = 0.03) in INR vs. AAC calves during preconditioning, and no treatment effects were noted (P = 0.76) for preconditioning average daily gain (ADG). Puberty attainment was hastened in AAC heifers during the experiment (treatment × day; P < 0.01), despite similar (P = 0.39) ADG between treatments from d 412 to 620. Expression of myogenin mRNA in the longissimus muscle was greater (P = 0.05) in INR vs. AAC heifers on d 584. No treatment effects were detected (P ≥ 0.24) for steer ADG from d 412 until slaughter, nor for carcass quality traits. Hepatic mRNA expression of metallothionein 1A was greater (P = 0.02) in INR vs. AAC steers on d 586. In summary, supplementing Co, Cu, Zn, and Mn as organic complexed instead of sulfate sources to beef cows during the last 5 mo of gestation did not improve performance and physiological responses of the steer progeny until slaughter, but hastened puberty attainment in the female progeny reared as replacement heifers.

PSXIII-17 Supplementing organic-complexed or inorganic Co, Cu, Mn, and Zn to beef cows during gestation: post-weaning responses of offspring reared as replacement heifers or feeder cattle

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 432-432
Author(s):  
Kelsey Harvey ◽  
Reinaldo F Cooke ◽  
Eduardo Colombo ◽  
Bruna Rett ◽  
Osvaldo Sousa ◽  
...  
Keyword(s):  
Treatment Effects ◽  
Body Condition Score ◽  
Average Daily Gain ◽  
Beef Cows ◽  
Female Progeny ◽  
Feeder Cattle ◽  
Condition Score ◽  
Haptoglobin Concentration ◽  
Eden Prairie ◽  
To Receive

Abstract Beef cows and their progeny were used to evaluate the effects of supplementing organic-complexed or inorganic Co, Cu, Mn, and Zn during gestation: on offspring reared as replacement heifers or feeder cattle. Non-lactating, pregnant beef cows (n = 190) were ranked by parity, pregnancy type, body weight (BW) and body condition score (BCS), at 117 ± 2.2 d of gestation (d 0) and assigned to receive a supplement containing: 1) sulfate sources of Cu, Co, Mn, and Zn (INR; n = 95) or 2) an organic complexed source of Cu, Mn, Co, and Zn (AAC; Availa®4; Zinpro Corporation, Eden Prairie, MN; n = 95). From d 0 to calving, cows were maintained in a single pasture and individually offered treatments three times a week. Calves were weaned at 6 mo of age (d 367) and preconditioned for 45-d. Heifer puberty status was verified weekly (d 437 to d 619) based on plasma progesterone concentrations. Steer calves were shipped to a commercial feedlot on d 493, where they were managed as a single group until slaughter (d 724). Plasma cortisol concentration was greater (P = 0.05) in AAC calves at weaning but tended to be less (P = 0.10) on d 370 compared with INR calves. Mean plasma haptoglobin concentration was greater (P = 0.03) in INR vs. AAC calves during preconditioning, and no treatment effects were noted (P = 0.76) for preconditioning average daily gain (ADG). Puberty attainment was hastened in AAC heifers during the experiment (treatment × day; P < 0.01), despite similar (P = 0.39) ADG between treatments from d 412 to 620. No treatment effects were detected (P ≥ 0.24) for steer ADG from d 412 until slaughter, nor for carcass quality traits. Therefore, supplementing Co, Cu, Zn, and Mn as organic complexed instead of sulfate sources to beef cows during the last 5 mo of gestation did not improve performance of steer progeny until slaughter, but hastened puberty attainment in female progeny reared as replacement heifers.

39 EFFECT OF TIME OF FIXED-TIME AI ON PREGNANCY RATES IN ZEBU CROSS-BRED BEEF HEIFERS TREATED WITH PROGESTERONE RELEASING DEVICES AND ESTRADIOL CYPIONATE

2010 ◽  
Vol 22 (1) ◽  
pp. 177 ◽  
Author(s):  
M. Ramos ◽  
L. Cutaia ◽  
P. Chesta ◽  
G. A. Bó
Keyword(s):  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Beef Heifers ◽  
Treatment Groups ◽  
Condition Score ◽  
To Receive

Two experiments were designed to evaluate the effect of the timing of fixed-time AI (FTAI) in relation to the removal of an intravaginal progesterone-releasing device (1 g of progesterone, DIB, Syntex SA, Buenos Aires, Argentina) on pregnancy rates in Bos indicus × Bos taurus cross-bred heifers. In experiment 1, 285 Bonsmara × zebu cross-bred heifers, between 18 and 24 months of age and with a body condition score (BCS) between 3.0 and 3.5 (1-5 scale) were used. On the day of initiation of treatment (Day 0), the heifers’ ovaries were palpated (92% of them had a CL) and they received a new DIB plus 2 mg of estradiol benzoate (EB; Syntex SA) and 250 μg of cloprostenol (Ciclase DL, Syntex SA). On Day 8, DIB devices were removed and all heifers received 250 μg of Ciclase plus 0.5 mg of estradiol cypionate (ECP; Cipiosyn, Syntex SA). At that time the heifers were randomly divided to receive FTAI between 48 to 49 h, 53 to 54 h, or 58 to 59 h after DIB removal. The heifers underwent FTAI with semen from 4 bulls by 2 inseminators. In experiment 2, 260 heifers from the same group as those used in experiment 1 (87% with a CL) were treated exactly as those in experiment 1, except that previously used DIB was inserted on Day 0. Pregnancy diagnosis was performed 30 days post-fixed-time AI by ultrasonography. The data were analyzed by logistic regression, taking into account the effect of time of FTAI, semen, and inseminator on pregnancy rates. In experiment 1, pregnancy rates were lower (P = 0.04) in the heifers undergoing FTAI between 48 and 49 h after DIB removal (46/95, 48.4%) than those undergoing FTAI 53 to 54 h (61/99, 61.6%) or 58 to 60 h (57/91, 62.6%) after DIB removal. However, no differences in pregnancy rates were found (P = 0.72) in experiment 2 between the 3 treatment groups, with 39/91 (42.9%) for the 48 to 49 h group, 45/89 (50.6%) for the 53 to 54 h group, and 35/89 (43.8%) for the 58 to 59 h group. There was no effect of the semen or inseminator (P > 0.2) in either experiment. We conclude that when Bos indicus × Bos taurus beef heifers are synchronized with new DIB devices and ECP, higher pregnancy rates are obtained in heifers undergoing FTAI late (between 53 to 60 h after DIB removal) than in those undergoing FTAI early (48 to 49 h after DIB removal). However, time of insemination does not apparently affect pregnancy rates when Bos indicus × Bos taurus beef heifers are synchronized with previously used DIB devices and ECP.

PSXIII-16 Supplementing organic-complexed or inorganic Co, Cu, Mn, and Zn to beef cows during gestation: physiological and productive responses of cows and their offspring until weaning

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 432-433
Author(s):  
Kelsey Harvey ◽  
Reinaldo F Cooke ◽  
Eduardo Colombo ◽  
Bruna Rett ◽  
Osvaldo Sousa ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Mrna Expression ◽  
Milk Production ◽  
Treatment Effects ◽  
Beef Cows ◽  
Longissimus Muscle ◽  
Liver Biopsies ◽  
Cow Calf ◽  
To Receive ◽  
Cu And Zn

Abstract Pregnant, non-lactating beef cows (n = 190; 117 ± 2.2 d of gestation; d 0) were assigned to receive: 1) sulfate sources of Cu, Co, Mn, and Zn (INR; n = 95) or 2) an organic complexed source of Cu, Mn, Co, and Zn (AAC; Availa®4, Zinpro; n = 95). From d 0 to calving, cows were maintained in a single pasture and individually offered treatments three times weekly. Cow BW and BCS were recorded on d -30, d 97, upon calving, and at weaning and liver biopsies performed in 30 cows/treatment. Milk production was estimated at 42 ± 0.5 d postpartum via weigh-suckle-weigh (WSW). Liver and longissimus muscle (LM) biopsies were performed in 30 calves/treatment upon calving and 24 h later, at WSW, and at weaning. No treatment effects were detected (P ≥ 0.49) for cow BCS, despite AAC cows having greater (P = 0.04) BW on d 97. Liver Co (P < 0.01) concentrations were greater for AAC compared to INR cows, and liver concentrations of Cu were greater (P = 0.02) for INR compared to AAC cows on d 97. Upon calving, INR cows had greater (P ≤ 0.01) liver Cu and Zn concentrations compared to AAC cows. Cows receiving AAC had greater (P = 0.04) hepatic mRNA expression of metallothionein 1A at calving, and their calves had greater (P = 0.04) hepatic mRNA expression of superoxide dismutase at weaning. Milk production did not differ between AAC and INR cows (P = 0.70). No treatment effects were detected (P ≥ 0.29) for mRNA expression of LM genes in calves at birth and weaning. Calf birth and weaning BW were similar (P ≥ 0.19) between treatments. Hence, supplementing AAC or INR to beef cows during the last 5 mo of gestation yielded similar cow-calf productive responses until weaning.

scholarly journals 158 PREGNANCY RATES IN RECIPIENT COWS TREATED WITH PROGESTERONE VAGINAL DEVICES AND INDUCED TO OVULATE WITH ESTRADIOL BENZOATE GIVEN AT THE TIME OF DEVICE REMOVAL OR 24 h LATER

2005 ◽  
Vol 17 (2) ◽  
pp. 229 ◽  
Author(s):  
G.A. Bo ◽  
E. Balla ◽  
L. Cutaia ◽  
L. Peres ◽  
P.S. Baruselli ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Beef Cows ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Device Removal ◽  
The Mean ◽  
To Receive

Although treatments with progesterone (P4) releasing devices, estradiol benzoate (EB) and eCG have been shown to result in acceptable pregnancy rates after embryo transfer, the treatment requires that the cows be run through the chute at least four times for treatments. An experiment was designed to compare pregnancy rates in cows treated with P4 releasing devices plus EB and eCG, and induced to ovulate with EB given at device removal or 24 h later. Non-lactating Bos taurus × Bos indicus crossbred beef cows (n = 165), with a body condition score between 2.5 to 3.5 (1 to 5 scale), were treated with a P4-device (DIB, Syntex, Argentina) and 2 mg EB i.m. (Syntex), on Day 0 and 400 IU of eCG i.m. (Novormon, Syntex) plus 150 μg D(+)cloprostenol i.m. (Ciclase, Syntex) on Day 5. On Day 8, DIB devices were removed and cows were randomly divided into two groups to receive either 1 mg EB i.m. at the time of DIB removal (EB0) or 24 h later (Day 9; EB24). Recipients were observed for signs of estrus for 48 h after DIB removal. On Day 16, all recipients observed in estrus and with >1 CL or a single CL with an area >256 mm2 were selected to receive fresh embryos on Day 16 (EB0) or Day 17 (EB24). Furthermore, 20 recipients not observed in estrus but with a CL >256 mm2 were randomly selected and transferred. The embryos used were 28 Grade 1, 40 Grade 2, and 24 Grade 3. Ovarian ultrasonography was performed on Day 0, to determine ovarian status (only cows with a CL or a follicle >10 mm and uterine tone were used), on Day 16 to measure CL area, and 60 days after embryo transfer to determine pregnancy status. Quantitative data were analyzed by Student's t-test and qualitative data were analyzed by logistic regression. There were no differences between groups in the mean (± SEM) CL area on Day 16 (EB0: 300.3 ± 12.0 mm2 and EB24: 324.9 ± 11.7 mm2; P = 0.14), the proportion of recipients in estrus (EB0: 38/82, 46.3%, and EB24: 34/83, 41.0%; P = 0.49), the number of recipients with >1 CL or a CL >256 mm2 (EB0: 68/82, 82.9%, and EB24: 72/83, 86.7%; P = 0.49) and the number of recipients pregnant/transferred (EB0: 31/49, 63.4%, and 20/43, 46.5%; P = 0.23). Furthermore, there were no significant effects of embryo quality (P = 0.31) or technician (P = 0.12) on pregnancy rates. The mean interval from DIB removal to estrus was shorter (P = 0.001) for recipients in the EB0 group (22.7 ± 1.0 h) than for those in the EB24 group (37.4 ± 1.2 h). Nevertheless, pregnancy rates did not differ (P = 0.14) between recipients seen in estrus (EB0: 21/38, 55.3%, and EB 24: 16/34, 47.1%) and those not seen in estrus but with a CL >256 mm2 on Day 16 (EB0: 10/11, 90.9%, and EB24 4/9, 44.4%). It was concluded that the two treatments evaluated are equally efficacious for synchronizing Bos taurus × Bos indicus recipients. Furthermore, the use of EB at device removal could reduce the number of trips through the chute without affecting pregnancy rates. Research was supported by Syntex S.A., Estancia El Mangrullo S.A., and Agencia Cordoba Ciencia S.E.

20 PREGNANCY RATES IN SUCKLED BEEF COWS SYNCHRONIZED WITH PROGESTERONE INTRAVAGINAL DEVICES AND RECEIVING eCG FOURTEEN DAYS AFTER BREEDING

2010 ◽  
Vol 22 (1) ◽  
pp. 168 ◽  
Author(s):  
L. Cutaia ◽  
M. Ramos ◽  
P. Chesta ◽  
G. A. Bó
Keyword(s):  
Body Condition ◽  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Fixed Time ◽  
Beef Cows ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Treatment Groups ◽  
Condition Score

An experiment was designed to test the following hypotheses: (1) the administration of 400IU of eCG (Novormón, Syntex SA, Buenos Aires, Argentina) 14 days after fixed-time artificial insemination (FTAI) increases pregnancy rates in cross-breed Zebu × Bonsmara suckled cows synchronized with progesterone releasing devices (DIB 0.5, 0.5 g of progesterone, Syntex SA); and (2) pregnancy rates in cows treated with estradiol or GnRH, combined with a DIB device, are not different. Primiparous suckled cows (n = 260), between 60 and 120 days postpartum (DPP) and with a body condition score between 2.0 and 2.5 (1 to 5 scale) were used. At the beginning of the experiment (Day 0), all cows were examined by rectal palpation (only 11% had a CL), received a DIB device, and were randomly allocated to 1 of 3 treatment groups. Cows in the E2 group received 2 mg of estradiol benzoate (EB) intramuscularly (i.m.) at DIB insertion and those in the two GnRH groups received 100 μg of gonadorelin (GDR Gonasyn, Syntex SA) at the same time. On Day 7, DIB devices were removed and all cows received 500 μg of cloprostenol (Ciclase DL, Syntex SA) plus 400 IU of eCG (i.m.). Cows in the E2 group received 0.5 mg of estradiol cypionate (ECP, Cipiosyn, Syntex SA) and underwent FTAI administered by one technician, using semen from 1 of 2 bulls between 52 and 56 h later. Cows in the GnRH groups received a dose of 100 μg of gonadorelin (full GnRH group) or 50 μg of gonadorelin (1/2 GnRH group) at the time of the FTAI (52 to 56 h after DIB removal). Fourteen days after FTAI cows in the 3 groups were further subdivided (3 × 2 factorial) to receive or not 400 IU of eCG i.m. Pregnancy was determined 30 days after FTAI using real-time ultrasonography. Data were analyzed by logistic regression, taking into account the effect of the synchronization treatment, eCG or noteCG, 14 days after FTAI and sire used. No differences in pregnancy rates were found (P = 0.16) among treatment groups (E2: 39/87, 43.7%, full GnRH: 38/92, 41.3%, and 1/2 GnRH: 25/81, 30.9%). However, there was a significant (P = 0.02) effect of eCG treatment 14 days after FTAI (eCG: 60/127, 47.2% v. no eCG: 41/133, 30.8%). Furthermore, bull A (77/155, 47.1%) resulted in higher pregnancy rate (P = 0.009) than bull B (28/107, 26.2%), but there were no significant interactions between bull and synchronization treatment or eCG administration 14 days after FTAI (P > 0.1). We conclude that the application of 400 IU of eCG 14 days after FTAI increases pregnancy rates in suckled Bos taurus × Bos indicus primiparous cows with fair to poor body condition score synchronized with progesterone releasing devices. Furthermore, the use of estradiol ester or GnRH at DIB insertion and removal did not affect pregnancy rates.

Evaluation of Bos indicus and Bos taurus straightbreds and crosses. II. Post-weaning growth, puberty, and pelvic size of heifers

1994 ◽  
Vol 45 (4) ◽  
pp. 795 ◽  
Author(s):  
H Hearnshaw ◽  
PF Arthur ◽  
R Barlow ◽  
PJ Kohun ◽  
RE Darnell
Keyword(s):  
Body Condition ◽  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
The Other ◽  
South Wales ◽  
Condition Score ◽  
Medium Quality ◽  
The Mean ◽  
Pelvic Size

Post-weaning growth and body condition, puberty and pelvic size of 197 heifers comprising straightbred Hereford (HxH) and Brahman (BxB), first-cross (BxH) and back-cross (HxBH and BxBH) heifers were evaluated. The heifers were born over a 3 year period, and grazed improved and semi-improved pastures following weaning at Grafton, New South Wales. Prior to weaning, heifers had been reared by dams on three pasture systems (high, medium and low quality pastures). Heifers from low quality pre-weaning pasture had higher (P < 0.05) post-weaning liveweight gain than those from high and medium quality pastures. BxH heifers gained 71 g/day more (P < 0.05) than the mean gain of their contemporaries of the other genotypes, whose gains were similar, from weaning to either 26 or to 30 months of age. Liveweight at all ages was influenced by genotype x pre-weaning pasture system interaction. At 30 months of age, BxH heifers from high and medium pre-weaning pastures were the heaviest. At the same age, but from low quality pre-weaning pasture, heifers with crossbred dams (HxBH and BxBH) were the heaviest. Wither height depended significantly (P < 0.05) on the proportion of Bos indicus genes, increasing from 113.8 cm in the HxH heifers to 124.4 cm in the BxB heifers at 30 months of age. BxH heifers had a higher (P < 0.05) body condition score than their contemporaries of the other genotypes, which were in similar condition, at 26 and 30 months of age. On average (across pre-weaning pasture system), 9% of BxB heifers had reached puberty by 22 months of age compared to 62, 95, 82 and 64% (s.e.= 9) for HxH, HxBH, BxH and BxBH heifers respectively. No significant genotype differences were obtained in the height, width and size of the pelvic opening of the heifers, measured just prior to the beginning of the mating season at 26 months of age.

207 INFLUENCE OF THE INTERVAL BETWEEN THAWING TO TRANSFER ON PREGNANCY RATES OF FROZEN - THAWED DIRECT-TRANSFER EMBRYOS

2007 ◽  
Vol 19 (1) ◽  
pp. 220
Author(s):  
G. A. Bo ◽  
L. C. Peres ◽  
D. Pincinato ◽  
M. de la Rey ◽  
R. Tribulo
Keyword(s):  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Direct Transfer ◽  
Condition Score ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

An experiment was designed to evaluate the effect of the interval between thawing to deposition of the embryo into the uterine horn on pregnancy rates of in vivo-produced frozen–thawed embryos in 1.5 M ethylene glycol (direct transfer). Data were collected from 1122 embryo transfers performed in the same farm (Estancia El Mangrullo, Lavalle, Santiago del Estero, Argentina) during the spring and summer of 2004/05 and 2005/06 (6 replicates, ambient temperature between 20 and 40�C). Recipients used in all replicates were non-lactating, cycling, multiparous Bos taurus � Bos indicus crossbred cows with body condition score between 3 and 4 (1 to 5 scale) that were synchronized using fixed-time embryo transfer protocols. Briefly, the synchronization treatments consisted of the insertion of a Crestar ear implant (Intervet, Sao Paulo, Brazil) or a progesterone-releasing device (DIB; Syntex SA, Buenos Aires, Argentina), plus 2 mg of estradiol benzoate (EB; Syntex) intramuscularly (IM) on Day 0, and 400 IU of eCG (Folligon 5000; Intervet, or Novormon 5000; Syntex) IM plus 150 �g d-cloprostenol IM (Preloban; Intervet, or Ciclase; Syntex) on Day 5. Progestin devices were removed on Day 8 and all cows received 1 mg of EB IM on Day 9. All cows were examined by ultrasonography on Day 16 and those with a luteal area &gt;76 mm2 (by calculating the area of the CL minus the area of the cavity) received, on Day 17, frozen–thawed embryos by nonsurgical transfer. All embryos were Grade 1, and all were frozen in 1.5 M ethylene glycol at the Embryo Plus Laboratory (Brits, South Africa). After being stored in liquid nitrogen, the embryos were plunged directly (no air thawing) in a 30�C water bath for 30 s, and then transferred to the recipient cows by either one of two technicians. Based on the interval between thawing and transfer, the transfers were classified as being in one of 3 groups: Group 1: &lt;3 min; Group 2: 3 to 6 min; and Group 3: 6 to 16 min. The main reason for delayed transfers beyond 6 min was the replacement of one recipient for another because of difficulty in threading the cervix (1% of the total transfers) or a recipient falling down into the chute or with very bad disposition and behavior. Pregnancy was determined by ultrasonography 28 to 35 days after fixed-time embryo transfer, and data were analyzed by logistic regression. There were no effects of replicate, technician, CL area, recipient body condition score, embryo stage, and time from thawing to transfer on pregnancy rates. Pregnancy rates in the 3 thawing to transfer intervals were: Group 1: 215/385, 55.8%; Group 2: 372/655, 56.8%; Group 3: 42/82, 51.2%; P &gt; 0.6. These results may be interpreted to suggest that there is no significant effect of time from thawing to transfer (up to 16 min) in direct transfer embryos using Bos taurus � Bos indicus recipients transferred at a fixed time.

Sign in / Sign up

Export Citation Format

Share Document