PSXIII-16 Supplementing organic-complexed or inorganic Co, Cu, Mn, and Zn to beef cows during gestation: physiological and productive responses of cows and their offspring until weaning

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 432-433
Author(s):  
Kelsey Harvey ◽  
Reinaldo F Cooke ◽  
Eduardo Colombo ◽  
Bruna Rett ◽  
Osvaldo Sousa ◽  
...  

Abstract Pregnant, non-lactating beef cows (n = 190; 117 ± 2.2 d of gestation; d 0) were assigned to receive: 1) sulfate sources of Cu, Co, Mn, and Zn (INR; n = 95) or 2) an organic complexed source of Cu, Mn, Co, and Zn (AAC; Availa®4, Zinpro; n = 95). From d 0 to calving, cows were maintained in a single pasture and individually offered treatments three times weekly. Cow BW and BCS were recorded on d -30, d 97, upon calving, and at weaning and liver biopsies performed in 30 cows/treatment. Milk production was estimated at 42 ± 0.5 d postpartum via weigh-suckle-weigh (WSW). Liver and longissimus muscle (LM) biopsies were performed in 30 calves/treatment upon calving and 24 h later, at WSW, and at weaning. No treatment effects were detected (P ≥ 0.49) for cow BCS, despite AAC cows having greater (P = 0.04) BW on d 97. Liver Co (P < 0.01) concentrations were greater for AAC compared to INR cows, and liver concentrations of Cu were greater (P = 0.02) for INR compared to AAC cows on d 97. Upon calving, INR cows had greater (P ≤ 0.01) liver Cu and Zn concentrations compared to AAC cows. Cows receiving AAC had greater (P = 0.04) hepatic mRNA expression of metallothionein 1A at calving, and their calves had greater (P = 0.04) hepatic mRNA expression of superoxide dismutase at weaning. Milk production did not differ between AAC and INR cows (P = 0.70). No treatment effects were detected (P ≥ 0.29) for mRNA expression of LM genes in calves at birth and weaning. Calf birth and weaning BW were similar (P ≥ 0.19) between treatments. Hence, supplementing AAC or INR to beef cows during the last 5 mo of gestation yielded similar cow-calf productive responses until weaning.

Author(s):  
Kelsey M Harvey ◽  
Reinaldo F Cooke ◽  
Eduardo A Colombo ◽  
Bruna Rett ◽  
Osvaldo A de Sousa ◽  
...  

Abstract One hundred and ninety non-lactating, pregnant beef cows (¾ Bos taurus and ¼ Bos indicus; 138 multiparous and 52 primiparous) were assigned to this experiment at 117 ± 2.2 d of gestation (d 0). Cows were ranked by parity, pregnancy type (artificial insemination = 102, natural service = 88), body weight (BW) and body condition score (BCS), and assigned to receive a supplement containing: 1) sulfate sources of Cu, Co, Mn, and Zn (INR; n = 95) or 2) an organic complexed source of Cu, Mn, Co, and Zn (AAC; Availa®4; Zinpro Corporation, Eden Prairie, MN; n = 95). The INR and AAC provided the same daily amount of Cu, Co, Mn, and Zn, based on 7 g of the AAC source. From d 0 to calving, cows were maintained in a single pasture and were segregated three times weekly into 1 of 24 individual feeding pens to receive treatments. Cow BW and BCS were recorded on d -30, d 97, upon calving, and at weaning (d 367). Milk production was estimated at 42 ± 0.5 d postpartum via weigh-suckle-weigh (WSW). Liver biopsies were performed in 30 cows/treatment on d -30, 97, upon calving, and the day after WSW. Calf BW was recorded at birth and weaning. Liver and longissimus muscle (LM) biopsies were performed in 30 calves/treatment upon calving and 24 h later, the day after WSW, and at weaning. No treatment effects were detected (P ≥ 0.49) for cow BCS during gestation, despite AAC cows having greater (P = 0.04) BW on d 97. Liver Co concentrations were greater (P < 0.01) for AAC compared to INR cows, and liver concentrations of Cu were greater (P = 0.02) for INR compared to AAC cows on d 97. Upon calving, INR cows had greater (P ≤ 0.01) liver Cu and Zn concentrations compared to AAC cows. No other treatment differences were noted (P ≥ 0.17) for cow and calf liver trace mineral concentrations. Cows receiving AAC had greater (P = 0.04) hepatic mRNA expression of metallothionein 1A at calving, and their calves had greater (P = 0.04) hepatic mRNA expression of superoxide dismutase at weaning. Milk production did not differ between AAC and INR cows (P = 0.70). No treatment effects were detected (P ≥ 0.29) for mRNA expression of LM genes associated with adipogenic or muscle development activities in calves at birth and weaning. Calf birth and weaning BW also did not differ (P ≥ 0.19) between treatments. In summary, supplementing Co, Cu, Zn, and Mn as organic complexed or sulfate sources to beef cows during the last 5 mo of gestation yielded similar cow-calf productive responses until weaning.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 51-52
Author(s):  
Alice Brandão

Abstract Option 1, Fetal Programming Only: Non-lactating, pregnant, multiparous Angus × Hereford cows (n = 104) were ranked by pregnancy sire (1 of 2 sires), body weight (BW), and body condition score (BCS) on d -15 of the experiment (d 180 of gestation). Cows were then assigned to receive: (1) 195 g/cow daily of CSSO (n = 52) or 2) 170 g/cow daily of CON (n = 52). Cows received treatments individually from d 0 to calving. Calves were weaned on d 290 of the experiment, preconditioned for 35 d, and placed in a feedyard until slaughter (d 514). Cows receiving CSSO and their calves had greater (P < 0.01) plasma concentrations ω-6 PUFA compared with CON after calving. Colostrum IgG and calf plasma IgG concentrations 24 h after birth were greater (P ≤ 0.02) in CSSO vs. CON cattle. Calves from CSSO cows had greater (P ≤ 0.05) expression of adipogenic and myogenic genes in the longissimus muscle compared with CON. No treatments differences in birth BW, weaning BW, and final preconditioning BW were noted (P ≥ 0.36). Average daily gain and final BW in the feedyard were greater (P ≤ 0.05) in steers from CSSO cows compared with CON. The incidence of calves diagnosed with BRD that required a second antimicrobial treatment was less (P = 0.03) in calves from CSSO cows, resulting in reduced (P = 0.05) need of treatments to regain health compared with CON. Upon slaughter, LM area was greater (P = 0.03) in calves from CSSO cows compared with CON. Collectively, these results are indicative of programming effects on postnatal offspring growth and health resultant from CSSO supplementation to late-gestating cows. Hence, supplementing CSSO to beef cows during pregnancy might be a feasible alternative to optimize offspring productivity and welfare. Option 2, Fetal Programming and Fat Tubs: Two experiments were conducted to evaluate: (1) effects of calcium salts of soybean oil (CSSO) supplementation to late gestating beef cows on the performance of the offspring; (2) the viability of utilizing low-moisture molasses-based blocks (LMB) as a delivery method for CSSO supplementation. In experiment 1, non-lactating, pregnant, multiparous Angus × Hereford cows were assigned to receive: (1) 195 g/cow daily of CSSO (n = 52) or (2) 170 g/cow daily of CON (n = 52) from d 195 of gestation until calving. CSSO cattle had greater (P < 0.01) plasma concentrations ω-6 PUFA; greater (P ≤ 0.02) colostrum and calf plasma IgG concentrations; greater (P ≤ 0.05) expression of adipogenic and myogenic genes in the longissimus muscle (LM); required fewer microbial treatments for BRD (P = 0.05) and had greater LM area compared to CON cohorts. In experiment 2, non-lactating, non-pregnant, multiparous beef cows were allocated to 1 of 9 pens (n = 9 pens; 4 cows/pen) and each pen was assigned to receive: 1) NOSUPP, hay only diet; 2) LMB, self-fed LMB supplement containing 24.7% DM CSSO; 3) CONC, hand-fed supplement identical to LMB. The design was a replicated 3 x 2 Latin square with 2 periods of 42 d, and a 21-d washout interval. Supplement DM intake did not differ (P = 0.39) between LMB and CONC cows from d 14 to 42 as designed, despite a greater variation in daily intake of LMB vs. CONC (treatment × day interaction; P < 0.01). No treatments effects were noted (P ≥ 0.40) for hay intake, BCS, and BW. From d 14 to 42, plasma concentrations of, ω-6 PUFA (P < 0.01) in CONC and LMB vs. NOSUPP cows. Hence, the use of self-fed LMB appears to be a valid strategy to provide CSSO to forage-fed beef cattle.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 432-432
Author(s):  
Kelsey Harvey ◽  
Reinaldo F Cooke ◽  
Eduardo Colombo ◽  
Bruna Rett ◽  
Osvaldo Sousa ◽  
...  

Abstract Beef cows and their progeny were used to evaluate the effects of supplementing organic-complexed or inorganic Co, Cu, Mn, and Zn during gestation: on offspring reared as replacement heifers or feeder cattle. Non-lactating, pregnant beef cows (n = 190) were ranked by parity, pregnancy type, body weight (BW) and body condition score (BCS), at 117 ± 2.2 d of gestation (d 0) and assigned to receive a supplement containing: 1) sulfate sources of Cu, Co, Mn, and Zn (INR; n = 95) or 2) an organic complexed source of Cu, Mn, Co, and Zn (AAC; Availa®4; Zinpro Corporation, Eden Prairie, MN; n = 95). From d 0 to calving, cows were maintained in a single pasture and individually offered treatments three times a week. Calves were weaned at 6 mo of age (d 367) and preconditioned for 45-d. Heifer puberty status was verified weekly (d 437 to d 619) based on plasma progesterone concentrations. Steer calves were shipped to a commercial feedlot on d 493, where they were managed as a single group until slaughter (d 724). Plasma cortisol concentration was greater (P = 0.05) in AAC calves at weaning but tended to be less (P = 0.10) on d 370 compared with INR calves. Mean plasma haptoglobin concentration was greater (P = 0.03) in INR vs. AAC calves during preconditioning, and no treatment effects were noted (P = 0.76) for preconditioning average daily gain (ADG). Puberty attainment was hastened in AAC heifers during the experiment (treatment × day; P < 0.01), despite similar (P = 0.39) ADG between treatments from d 412 to 620. No treatment effects were detected (P ≥ 0.24) for steer ADG from d 412 until slaughter, nor for carcass quality traits. Therefore, supplementing Co, Cu, Zn, and Mn as organic complexed instead of sulfate sources to beef cows during the last 5 mo of gestation did not improve performance of steer progeny until slaughter, but hastened puberty attainment in female progeny reared as replacement heifers.


2018 ◽  
Vol 3 (1) ◽  
pp. 443-455 ◽  
Author(s):  
Philipe Moriel ◽  
Bruno Ieda Cappellozza ◽  
Matheus B Piccolo ◽  
Reinaldo F Cooke ◽  
Miguel F Miranda ◽  
...  

Abstract The present study evaluated the growth and puberty attainment of Bos indicus heifers administered recombinant bovine somatotropin (bST) or saline injections during preweaning and/or postweaning. On day 0, 177 suckling Nellore heifers were stratified by initial age and body weight (BW) (80 ± 10 d; 97 ± 16 kg), and randomly assigned, in a 2 × 2 factorial design (n = 44 to 45 heifers/treatment), to receive s.c. injections of saline (5 mL 0.9% NaCl) or sometribove zinc (Posilac; Elanco, Greenfield, IN; 6.14 mg/kg of BW0.75) on days 0 and 10 (PRE) and/or days 167 and 177 (POS). All heifers were managed as a single group in Brachiaria decumbens pastures from day 0 until 24 d postweaning (day 191), and then provided a corn silage–based TMR from days 191 to 380 to achieve 65% to 70% of mature BW at the end of the study (day 380). Heifer full BW was collected on days 0, 10, 167, 177, and monthly from days 191 to 380. Transrectal ultrasonography of ovaries was performed on days 1 and 10 of each month from days 229 to 380 to assess the percentage of pubertal heifers. Liver biopsies and blood samples from jugular vein were collected on days 0, 10, 167, 177, and 380. Additional blood samples were collected monthly from days 259 to 380 (n = 10 to 15 heifers/treatment). No interactions among day of the study, PRE, and POS injections of saline or bST were detected (P ≥ 0.11). Preweaning bST injections increased heifer average daily gain (ADG) from days 0 to 10 and plasma IGF-1 on day 10 (P ≤ 0.03), did not affect ADG from days 0 to 177, plasma IGF-1 from days 259 to 380, and any liver gene mRNA expression (P ≥ 0.19), but tended to decrease ADG from days 191 to 380 (P = 0.07) and percentage of pubertal heifers on days 349 (P = 0.07), 359 (P = 0.002), and 380 (P = 0.0001) compared with saline injections. Postweaning bST injections increased plasma IGF-1 on day 177 and overall liver mRNA expression of GHR-1A (P ≤ 0.05), decreased plasma IGF-1 from days 259 to 380 (P = 0.03), tended to decrease liver mRNA expression of GHR-1B on day 177 (P = 0.08), but did not affect ADG from days 167 to 177 and 191 to 380, and puberty attainment from days 229 to 380 (P ≥ 0.12) compared with saline injections. Thus, preweaning and postweaning injections of bST successfully increased heifer plasma IGF-1 concentrations 10 d after first injection. Postweaning injections of bST had no impact on puberty attainment, whereas preweaning bST injections of bST impaired puberty attainment of Nellore beef heifers.


2017 ◽  
Vol 1 (1) ◽  
pp. 54-59 ◽  
Author(s):  
S. R. Edwards ◽  
J. D. Hobbs ◽  
J. T. Mulliniks

Abstract The beef cattle industry tends to focus on selecting production traits with the purpose of maximizing cow-calf performance. One such trait is milking ability, which is considered the primary influence on weaning weight of the calf. Therefore, the objective of this study was to determine the effect of actual milk yield on reproductive performance, circulating blood metabolites, and calf performance in beef cows in the Southeastern US. Over a 2 yr period, data were collected from 237, 3- to 9-yr-old Angus-sired beef cows on 3 research stations in Tennessee. On approximately d 58 and 129 postpartum, 24-hr milk production was measured with a modified weigh-suckle-weigh technique using a milking machine. Subsamples of milk were collected for analysis of milk components. Milk yield data were used to retrospectively classify cows on actual milk yield as High ( ≥ 10 kg/d), Mod (8 to 9 kg/d), or Low ( < 8 kg/d). Cow body weight (BW) and body condition score (BCS) were collected weekly at each location through breeding. Calf BW was recorded at birth, mid-weight at d 58, and weaning. At d 58 and 129 of postpartum, milk yields were different (P < 0.001) among the treatment groups. Cow BW during the entire study were not different (P ≥ 0.22) with increasing milk yield. Timed-AI pregnancy rate were the lowest (P = 0.02) in the High milk producing cows with no difference (P > 0.05) between Low and Mod milk cows. In addition, overall pregnancy rate continued to be the lowest (P = 0.04) in High milk producing cows with the greatest pregnancy rate in Mod milk cows. Calf mid-weight at ∼d 58 was increased (P < 0.001) in calves from Mod and High milking cows. However, calf BW at weaning was not different (P = 0.22) among calves from different milk treatment groups. Results from this study suggest that even in management systems that modify the grazing environments with harvested feedstuffs, high milk production decreases reproductive efficiency. In addition, increasing milk production up to d 129 postpartum did not result in increased calf BW at weaning, indicating that the genetic potential for calf BW at weaning could not be improved with increased genetic potential for milk production.


2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 52-53
Author(s):  
Alice Brandão ◽  
Reinaldo F Cooke ◽  
Kelsey Schubach ◽  
Thiago Schumaher ◽  
Osvaldo Souza ◽  
...  

Abstract Synthetic appeasing pheromone (SAP) is expected to have a calming effect in cattle experiencing stressful situations, including weaning and feedlot arrival. In Exp. 1, 186 Bos indicus-influenced calves (73 heifers, 133 bulls) were weaned at 211 ± 1 d of age (d 0). Upon weaning, calves were ranked by sex and body weight (BW), and assigned to receive SAP (Nutricorp, Araras, SP, Brazil; n = 94) or water (CON; n = 92). Treatments (5 mL) were topically applied to the forehead of each animal. Calf BW was recorded on d 15 and 45. Calves that received SAP had greater (P < 0.01) average daily again (ADG) from d 0 to 15, and BW on d 15 compared with CON (0.94 vs. 0.73 kg/d and 249 vs. 246 kg; respectively). Calf ADG from d 15 to 45 was similar (P = 0.97) between treatments. Overall ADG and BW on d 45 were also greater in SAP vs. CON (0.36 vs. 0.29 kg/d and 251 vs. 248 kg; respectively). In Exp. 2, 140 Bos indicus-influenced bulls (~27 mo of age) were transported from a cow-calf ranch to a commercial feedlot (d -1), and maintained on a single pasture for 24 h. On d 0, bulls were ranked by BW and assigned to receive SAP (n = 70) or CON (n = 70) as in Exp. 1. Bulls receiving SAP had greater (P = 0.04) ADG from d 0 to 15, but less (P <0.01) ADG from d 15 to 45 compared to CON (1.65 vs. 1.45 kg/d and 1.42 vs. 1.65 kg/d; respectively). No other treatment effects were detected (P ≥ 0.18). Therefore, SAP administration to beef cattle at weaning or feedlot entry had immediate benefits to ADG, which were sustained for 45 d in weaned calves but not in feedlot cattle


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 60-61
Author(s):  
Travis Mulliniks ◽  
Joslyn Beard ◽  
Tasha King

Abstract Cow herd profitability can be greatly influenced by reproductive success, average age of herd, and genetic potential within the environment. Productivity is influenced by cow age with calf weaning weight, increasing from 2 to 5 yr of age in dams. With that in mind, increasing average age of the cowherd has the potential to increase cow/calf profitability. Culling cows before 5 yr of age negatively impacts production efficiency due to decreased calf weaning weight in younger cows and subsequently, increase replacement rate and costs. Therefore, reproduction in young cattle is important to overall economic efficiency within the cow herd and a driving factor in longevity. Due to decreased reproduction and offspring performance, young females have previously been considered an economic liability. The effect of increased average cow age within the herd along with increased pregnancy rate of young cows has shown to increase production efficiency by decreasing replacement rates and adding greater production potential with increased calf weaning weights. In young beef cows, partitioning nutrients away from lactational demands allows for nutrients to be utilized for cow growth and reproduction. An increase in selection for milk production has resulted in beef cows undergoing greater nutritional stress during early lactation, which ultimately reduces cowherd reproduction and efficiency. Although a big emphasis is placed on output traits, economic value of reproduction is 5 times greater than growth or maternal output traits in beef cattle. In addition, the value of the added milk production may not fully be captured, due to extensive environments with limited nutrients. Even in environments with greater feed resources, selection for milk production may led to a decrease in reproduction, production efficiency, and cowherd retention rate.


Author(s):  
John D Arthington ◽  
Maria L Silveira ◽  
Luana S Caramalac ◽  
Henrique J Fernandes ◽  
Jeff S Heldt ◽  
...  

Abstract Three studies were completed to evaluate the effects of Cu, Zn, and Mn source on preferential intake, trace mineral status and rainfall-induced metal loss of salt-based mineral supplements. Mineral supplements were formulated to contain 2,500, 5,500, and 4,000 mg/kg of Cu, Zn, and Mn, respectively. Supplements differed only by source of Cu, Zn, and Mn, which were hydroxychloride, organic, or sulfate sources. In Exp. 1, the 3 formulations were offered simultaneously for 18 wk to pre-weaned beef calves (4 pastures; 17 calves/pasture) within separate containers inside covered cow-exclusion areas. Consumption averaged 21 ± 2.4 g/calf daily (sum of all 3 sources), with a greater (P &lt; 0.001) percentage of the total intake coming from the hydroxychloride- vs. organic- or sulfate-sources of Cu, Zn, and Mn. In Exp. 2, the same sulfate and hydroxychloride formulations were randomly assigned to pastures (n = 4 pastures/treatment) containing 18 to 20 cow-calf pairs/pasture. Treatments were offered for 20 wk within covered areas designed to assess cow and calf intake separately. At weaning, liver biopsies were collected from 4 cow-calf pairs/pasture (n = 16 cows and calves/treatment). Source of Cu, Zn, and Mn had no effect on voluntary mineral intake among calves (P = 0.44) and cows (P = 0.14). Calves consuming mineral containing hydroxychloride-sources of Cu, Zn, and Mn tended (P = 0.06) to have greater ADG over the 20-wk period compared to calves consuming sulfate-sources of the same elements (1.09 vs. 1.06 kg/d; SEM = 0.013). Mineral status of cows and calves was not affected (P ≥ 0.17) by source of Cu, Zn, and Mn. In Exp. 3, each of the mineral formulations from Exp. 1, were exposed to a 10.2 cm precipitation event delivered in three equal 3.4 cm applications within a week. To accomplish this, 750 g of mineral was placed into Buchner funnels (177 cm 2) on 20 to 25 µm pore filter paper. Deionized water (pH adjusted to 5.6) was poured over the mineral. Total leaching losses of Cu, Zn, and Mn were less (P &lt; 0.001) for formulations containing hydroxychloride- vs. organic- and sulfate-sources. These results imply that, when offered a choice, calves preferentially consume mineral supplements formulated with hydroxychloride vs. sulfate or organic sources of Cu, Zn, and Mn. In addition, hydroxychloride sources of Cu, Zn, and Mn are less susceptible to rainfall-induced leaching losses compared to sulfate and organic sources.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 149-149
Author(s):  
Allison Millican ◽  
Reinaldo F Cooke ◽  
Alice Brandão ◽  
Thiago Schumaher ◽  
Osvaldo Souza ◽  
...  

Abstract Bovine appeasing substance (BAS) is expected to have calming effect in cattle experiencing stressful situations, including weaning and feedlot arrival. In Exp. 1, 186 Bos indicus-influenced calves (73 heifers, 133 bulls) were weaned at 211 ± 1 d of age (d 0). Upon weaning, calves were ranked by sex and body weight (BW), and assigned to receive BAS (Nutricorp, Araras, SP, Brazil; n = 94) or water (CON; n = 92). Treatments (5-mL) were topically applied to the forehead of each animal. Calf BW was recorded and samples of blood and tail-switch hair collected on d 0, 15, and 45. Calves that received BAS had greater (P &lt; 0.01) average daily again (ADG) from d 0 to 15, and BW on d 15 compared with CON. Overall ADG and BW on d 45 were also greater (P &lt; 0.05) in BAS vs. CON. Plasma concentrations of haptoglobin were less (P &lt; 0.01) in BAS vs. CON cattle on d 15, whereas cortisol concentrations in plasma and tail-switch hair did not differ between treatments (P &gt; 0.16). In Exp. 2, 140 B. indicus-influenced bulls (~27 mo of age) were transported from a cow-calf ranch to a commercial feedlot (d -1), and maintained on a single pasture for 24 h. On d 0, bulls were ranked by BW and assigned to receive BAS (n = 70) or CON (n = 70) as in Exp. 1. Bulls receiving BAS had greater (P = 0.04) ADG from d 0 to 15, but less (P &lt; 0.01) ADG from d 15 to 45 compared to CON. No other treatment effects were detected (P &gt; 0.18). Therefore, BAS administration to beef cattle at weaning or feedlot entry had immediate benefits to ADG, which were sustained for 45 d in weaned calves but not in feedlot cattle.


Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2331
Author(s):  
Carmen J. Willmore ◽  
John B. Hall ◽  
Mary E. Drewnoski

The objective was to determine the effects of an injectable trace mineral (TMI; Multimin 90) containing copper (Cu), manganese (Mn), selenium (Se), and zinc (Zn) on trace mineral status and the resulting impacts on reproduction of beef cows and the growth of their calves. Beef cows (n = 200) were assigned to receive TMI or no injection (CON) prior to calving and breeding over two consecutive years. Calves born to cows receiving TMI also received TMI at birth in both years and at 49 ± 1.3 days of age in year 1. The TMI increased (p = 0.01) liver Zn and tended (p = 0.06) to increase liver Cu concentrations. Short-lived effects of TMI on Se were observed. Liver Cu and Zn would have been considered adequate and Se marginal in the CON. Pregnancy due to artificial insemination and overall pregnancy rate did not differ (p ≥ 0.36) between treatments. Use of TMI did not increase calf pre-weaning gain. These data indicate that TMI does not improve the reproductive performance of beef cows with adequate trace mineral status or the pre-weaning performance of their calves.


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