Mobility of Mass-Reared Diapaused and Nondiapaused Cydia pomonella (Lepidoptera: Tortricidae): Effect of Different Constant Temperatures and Lengths of Cold Storage

2006 ◽  
Vol 99 (3) ◽  
pp. 707-713 ◽  
Author(s):  
Stephanie Bloem ◽  
James E. Carpenter ◽  
Silvia Dorn
Keyword(s):  
Cold Storage ◽  
Cydia Pomonella

The effect of cold storage of mass-reared codling moths (Lepidoptera: Tortricidae) on subsequent flight capacity

2017 ◽  
Vol 149 (3) ◽  
pp. 391-398 ◽  
Author(s):  
E. Matveev ◽  
J.J. Kwon ◽  
G.J.R. Judd ◽  
M.L. Evenden
Keyword(s):  
Weight Loss ◽  
Cold Storage ◽  
Lipid Content ◽  
Energy Use ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Temperature Treatment ◽  
Flight Distance ◽  
Flight Capacity ◽  
Flight Mills

AbstractFlight capacity of codling moths, Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae), was measured with computer-linked flight mills following exposure to one of two temperature regimes. Codling moth adults were held for 24 hours before flight under either chilled (2 °C) or unchilled (24 °C) conditions. The chilling treatment emulated conditions that codling moths are exposed to pre-release in the Okanagan Sterile Insect Release (SIR) Programme. Moths were assayed on flight mills for eight hours to obtain a measure of flight capacity based on total flight distance. Energy use was assessed by weight loss during flight and post-flight body lipid content compared with similarly treated moths that did not fly. Flight distance did not vary with pre-flight temperature treatment, however moth pre-flight weight influenced flight capacity; heavier moths flew further. Moths chilled before the bioassay used less energy based on reduced weight loss and higher lipid content post bioassay as compared with unchilled moths. The current cold storage procedure used in the SIR Programme does not negatively influence subsequent flight capacity and reduces energy use during flight. Codling moths can fly on average between 7–10km in an eight-hour flight mill bioassay and lipids, at least in part, are used to fuel flight.

scholarly journals Efficacy of Using In-carton Fumigation with the Quarantine Treatment Against Codling Moth on Apples Intended for Export to Japan

2002 ◽  
Vol 12 (3) ◽  
pp. 441-443
Author(s):  
James D. Hansen ◽  
Dennis J. Albano ◽  
Millie L. Heidt
Keyword(s):  
Cold Storage ◽  
Methyl Bromide ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Post Evaluation ◽  
Confirmation Test ◽  
Two Component

The two-component quarantine treatment was shown to be effective against at least 7,000 codling moth (Cydia pomonella) fifth instar larvae infesting `Fuji' apples (Malus × domestica) in each required confirmation test involving two sizes of cartons. After cold storage for 55 days at 36 °F (2.2 °C), infested fruit were placed in vented cartons, either 20-lb [7 × 12 × 12.5 inches (17.8 × 30.5 × 31.8 cm)], or 40-lb [12 × 12.5 × 20.5 inches (30.5 × 31.8 × 52.1 cm)], then fumigated with 0.056 oz/ft3 (56 g·m-3) of methyl bromide for 2 hours at 50 °F (10.0 °C). After each treatment, either no survivors were present or no moribund larvae survived beyond the first week of post evaluation of the larvae.

Evaluation of a non-chemical disinfestation treatment on quality of pome fruit and mortality of lepidopterous pests

1999 ◽  
Vol 39 (3) ◽  
pp. 335 ◽  
Author(s):  
C. Chervin ◽  
S. L. Kreidl ◽  
P. R. Franz ◽  
A. J. Hamilton ◽  
S. R. Whitmore ◽  
...  
Keyword(s):  
Cold Storage ◽  
Life Stage ◽  
Cydia Pomonella ◽  
Cold Treatment ◽  
Codling Moth ◽  
Effective Control ◽  
Pome Fruit ◽  
Treatment Comparison ◽  
Lightbrown Apple Moth ◽  
Time Required

Australian pome fruit exports to northern America and Japan are rejected if they contain lightbrown apple moth (Epiphyas postvittana Walker) or codling moth (Cydia pomonella Linnaeus) respectively. A warm controlled atmosphere (CA) disinfestation treatment (72 h at 28˚C at 0, 1 or 2 kPa O2 ), followed by varying lengths of cold storage (5 weeks, 10 weeks or 6 months), is described. Four cultivars were studied: Packham’s Triumph pears (Pyrus communis Linnaeus); Royal Gala; Fuji and Pink Lady apples (Malus domestica Borkh.). Consumer panels found that fruit subjected to the 2 kPa O2 warm CA treatment and cold storage were as acceptable as control fruit. This is an important finding as previously published results for a harsher treatment described treated fruit (especially Pink Lady) as being less acceptable than controls. Some advanced ripening was observed for treated Packham’s Triumph pears. After 5 and 10 weeks cold storage, treated Royal Gala apples were generally firmer than the controls. Insects were also subjected to the 2 kPa O2 warm CA treatment. The most tolerant life stage (MTS) of lightbrown apple moth was the sixth instar with an LT95 of 37 days cold storage (0.5˚C) after the 2 kPa O2 warm CA treatment. Comparison with previous research suggests that the 2 kPa O2 warm CA treatment approximately halves the time required in cold storage for effective control of late instar lightbrown apple moth. A LT95 of 81 days was achieved for fifth instar codling moths (considered to be the MTS) and this may need to be reduced for export to Japan. Preliminary observations suggested that there may not be substantial difference between the resistance of non-diapausing and pre-diapausing codling moth larvae to the warm CA followed by cold treatment.

scholarly journals A Two-component Quarantine Treatment for Postharvest Control of Codling Moth on Apple Cultivars Intended for Export to Japan and Korea

2000 ◽  
Vol 10 (1) ◽  
pp. 186-194 ◽  
Author(s):  
James D. Hansen ◽  
Harold R. Moffitt ◽  
Dennis J. Albano ◽  
Millie L. Heidt ◽  
Stephen R. Drake ◽  
...  
Keyword(s):  
Cold Storage ◽  
Methyl Bromide ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Treatment Comparison ◽  
Two Component ◽  
Confirmatory Tests ◽  
Sigmoid Curve ◽  
Apple Cultivars ◽  
Regression Slopes

Confirmatory tests were performed on a two-component quarantine treatment against the codling moth (Cydia pomonella L.) (Lepidoptera: Tortricidae) for seven apple [Malus sylvestris (L.) var. domestica (Borkh.) Mansf.] cultivars ('Delicious,' `Golden Delicious,' `Braeburn,' `Fuji,' `Gala,' `Jonagold,' and `Granny Smith') intended for export to Japan and Korea. Treatment consists of a 55-day cold storage at 40 °F (2.2 °C) or below, followed by a 2-hour methyl bromide fumigation (0.056 oz/ft3 or 56 g·m-3) at 50 °F (10 °C). No eggs or larvae survived this treatment. Comparison tests were conducted on all cultivars to demonstrate no difference in insect responses between a previously accepted cultivar and proposed cultivars. Concentration-mortality responses were determined for each of the components and no statistical differences were found in the regression slopes of pest mortality with controlling variable (either cold exposure or fumigation) among all cultivars. Descriptive mathematical models, developed for the effects of cold storage on egg mortality and for methyl bromide fumigation on larvae mortality, were sigmoid curve equations.

scholarly journals Cold Storage to Control Codling Moth Larvae in Fresh Apples

2007 ◽  
Vol 17 (2) ◽  
pp. 195-198 ◽  
Author(s):  
J.D. Hansen ◽  
M.A. Watkins ◽  
M.L. Heidt ◽  
P.A. Anderson
Keyword(s):  
Cold Storage ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
International Markets ◽  
Malus Sylvestris

Codling moth [Cydia pomonella (Lepidoptera: Tortricidae)], found in exported apples (Malus sylvestris), can disrupt international markets. Cold storage at 1.1 °C was examined for possible control of three physiological larval states in ‘Fuji’ apples: diapausing (overwintering), diapause-destined, and nondiapausing. All nondiapausing larvae were dead within 12 weeks, diapaused-destined larvae were controlled by the seventh week, yet more than half of the original populations of diapausing larvae were still alive after 11 weeks. Because the diapaused-destined larvae were younger than the nondiapausing larvae, they may have been more susceptible to cold. Because larvae normally diapause outside the fruit, cold storage would not be applicable for controlling larvae in this state.

Effect of a Combined Methyl Bromide Fumigation and Cold Storage Treatment on Cydia pomonella (Lepidoptera: Tortricidae) Mortality on Apples

1998 ◽  
Vol 91 (2) ◽  
pp. 528-533 ◽  
Author(s):  
Peter R. Dentener ◽  
Sharon M. Alexander ◽  
Robert J. Petry ◽  
Glenda M. O'Connor ◽  
Philip J. Lester ◽  
...  
Keyword(s):  
Cold Storage ◽  
Methyl Bromide ◽  
Cydia Pomonella ◽  
Storage Treatment

Structural integrity after cold storage of human epidermal model in hypothermosol: A correlative ultrastructural and fluorescent assay

1994 ◽  
Vol 52 ◽  
pp. 270-271
Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk
Keyword(s):  
Cold Storage ◽  
Structural Integrity ◽  
Culture Media ◽  
Cell Structure ◽  
Natural State ◽  
Sodium Cacodylate ◽  
Ruthenium Tetroxide ◽  
Cacodylate Buffer ◽  
Before And After

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.

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