scholarly journals First Report of Human Myiasis Caused by Chrysomya megacephala and Chrysomya rufifacies (Diptera: Calliphoridae) in Thailand, and Its Implication in Forensic Entomology

2005 ◽  
Vol 42 (4) ◽  
pp. 702-704 ◽  
Author(s):  
Kabkaew L. Sukontason ◽  
Paitoon Narongchai ◽  
Duanghatai Sripakdee ◽  
Noppawan Boonchu ◽  
Tarinee Chaiwong ◽  
...  
Keyword(s):  
Forensic Entomology ◽  
Chrysomya Rufifacies ◽  
Chrysomya Megacephala ◽  
First Report

First Report of Human Myiasis Caused by Chrysomya megacephala and Chrysomya rufifacies (Diptera: Calliphoridae) in Thailand, and Its Implication in Forensic Entomology

2005 ◽  
Vol 42 (4) ◽  
pp. 702-704 ◽  
Author(s):  
Kabkaew L. Sukontason ◽  
Paitoon Narongchai ◽  
Duanghatai Sripakdee ◽  
Noppawan Boonchu ◽  
Tarinee Chaiwong ◽  
...  
Keyword(s):  
Forensic Entomology ◽  
Chrysomya Rufifacies ◽  
Chrysomya Megacephala ◽  
First Report

scholarly journals POLA SERANGGA NEKROFAGUS PADA DEKOMPOSISI HEWAN COBA INDOORS DI KOTA MANADO

2014 ◽  
Vol 6 (2) ◽  
Author(s):  
Sunny Wangko ◽  
Erwin G. Kristanto ◽  
Sonny J. R. Kalangi
Keyword(s):  
Late Stage ◽  
Coastal Area ◽  
Early Stage ◽  
Chrysomya Rufifacies ◽  
Domestic Pig ◽  
Hermetia Illucens ◽  
Chrysomya Megacephala ◽  
First Report ◽  
Dermestes Maculatus ◽  
Pmi Estimation

Abstract: Studies of insect succession in indoor carcasses are still very rare reported. This study aimed to obtain the succession pattern of necrophagus insects in indoor carcass in Manado. One domestic pig was used as model, and killed with a cardiac puncture. The results showed that the duration of decomposition took 16 days to reach skeletonization. There were 3 orders found, as follows: Diptera, Coleoptera, and Hymenoptera, consisting of  8 families and 12 species. Albeit, insects that colonized in the carcasses were Chrysomya rufifacies, Chrysomya megacephala, Sarcophaga harpax, Hermetia illucens, Dermestes maculatus, and several species of Muscidae. Both species of Chrysomya and Sarcophaga harpax can be applied in PMI estimation at early stage of decomposition meanwhile Hermetia illucens and Dermestes maculatus at late stage of decompostion. To our knowledge, this is the first study of decomposition and insect succession indoors in a coastal area in Indonesia. Moreover, this is the first report of Sarcophaga harpax which is biomolecularly identified with mtDNA in Indonesia. Further studies are needed to support the database of forensic insects in Indonesia, especially in Manado. Keywords: decomposition, indoors, necrophagus insects, colonization     Abstrak: Studi mengenai suksesi serangga indoors masih sangat jarang dilaporkan. Penelitian ini bertujuan untuk mendapatkan pola serangga nekrofagus pada bangkai hewan coba indoors di Manado. Sebagai hewan coba digunakan satu ekor babi domestik yang dimatikan dengan tusukan pada jantung. Hasil penelitian memperlihatkan dekomposisi hewan coba indoors di kota Manado memerlukan 16 hari untuk mencapai skeletonization. Serangga yang berkunjung ke hewan coba tergolong dalam 3 ordo, yaitu: Diptera, Coleoptera, dan Hymenoptera, yang terdiri atas 8 famili dan 12 spesies. Serangga yang ditemukan berkolonisasi pada bangkai hewan coba ialah Chrysomya rufifacies, Chrysomya megacephala, Sarcophaga harpax, Hermetia illucens, Dermestes maculatus, dan beberapa spesies Muscidae. Kedua spesies Chrysomya dan Sarcophaga harpax dapat dimanfaatkan dalam perkiraan PMI pada dekomposisi awal, sedangkan Hermetia illucens dan Dermestes maculatus dapat dimanfaatkan pada dekomposisi lanjut. Penelitian mengenai dekomposisi dan suksesi serangga indoors ini merupakan yang pertama di daerah pesisir di Indonesia. Terdapatnya Sarcophaga harpax yang diidentifikasi secara biomolekular dengan mtDNA juga merupakan yang pertama kali dilaporkan di Indonesia. Studi lanjut dibutuhkan untuk menyokong database serangga forensik di Indonesia, khususnya Manado. Kata kunci: dekomposisi, indoors, serangga nekrofagus, kolonisasi

Morphology and developmental rate of blowflies Chrysomya megacephala and Chrysomya rufifacies in Thailand: application in forensic entomology

2008 ◽  
Vol 102 (6) ◽  
pp. 1207-1216 ◽  
Author(s):  
Kom Sukontason ◽  
Somsak Piangjai ◽  
Sirisuda Siriwattanarungsee ◽  
Kabkaew L. Sukontason
Keyword(s):  
Forensic Entomology ◽  
Developmental Rate ◽  
Chrysomya Rufifacies ◽  
Chrysomya Megacephala

Effect of Type of Tissue on the Development of Chrysomya rufifacies (Diptera: Calliphoridae) in Sri Lanka

2021 ◽  
Author(s):  
Y T B Bambaradeniya ◽  
W A I P Karunaratne ◽  
J K Tomberlin ◽  
P A Magni
Keyword(s):  
Skeletal Muscle ◽  
Sri Lanka ◽  
Forensic Entomology ◽  
Development Time ◽  
Developmental Studies ◽  
Chrysomya Rufifacies ◽  
Significant Difference ◽  
The World ◽  
Significant Temperature ◽  
Domestic Swine

Abstract Chrysomya rufifacies (Macquart), the hairy maggot blow fly, is of great importance for the field of forensic entomology due to its habit as an early colonizer of decomposing vertebrate remains and myiasis producer. Development studies on this species have been conducted in scattered regions of the world, using types of tissue from several species of animals as a rearing medium. Despite the commonality of C. rufifacies in Sri Lanka, developmental studies have never been performed in this region. As well, the effects of diet on development have not been tested. In the current study, C. rufifacies immatures were reared on skeletal muscle, liver, and heart from domestic swine, with flies from colonies maintained at 25 and 28°C. The minimum time needed to complete each stage at 25°C on liver (224.14 h) was fastest followed by skeletal muscle (249.33 h) and heart (251.64 h) respectively, whereas at 28°C, fly development was quickest on heart muscle (178.27 h) followed by liver (178.50 h) and skeletal muscle (186.17 h) respectively. A significant difference in total development time was determined for temperature, while the rearing medium was not significant. Temperature also showed a significant effect on the length and the width of the larvae, while the type of tissue statistically impacted only the width.

scholarly journals The Diversity and Activity of Flies (Diptera: Cyclorrhapha) as Forensic Indicators at Outdoor and Indoor Rat Carccasses in Dramaga, Bogor

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Irwan Ismail ◽  
Yusuf Ridwan ◽  
Susi Soviana
Keyword(s):  
Musca Domestica ◽  
Rattus Norvegicus ◽  
Chrysomya Rufifacies ◽  
Chrysomya Megacephala ◽  
Decay Stage ◽  
Chrysomya Bezziana

This study aims to identify the diversity of flies that were caught and interacted with carcasses at the day and night. Each of the three rat carcasses (Rattus norvegicus) was placed outdoor and indoor within the campus of IPB Dramaga. The carcasses were put into a screen cage. Observation of flies was begun at 06:00 am and so on every four hours until the skeletal stage of the carcasses reached. The flies that come to the carcasses and into the screen cage were collected and identified. The decomposition of the indoor carcasses is slower than at outdoor, with the longer fresh stage. Meanwhile, post-decay stage and skeletal stage were achieved at the same time at each location. Nine spesies captured flies which are forensic indicator were Chrysomya megacephala (Fabricius), Chrysomya bezziana Villeneuve, Chrysomya rufifacies (Macquart), Chrysomya saffranea, Chrysomya spp, Lucilia spp, Sarcophaga spp, Calliphora spp and Musca domestica. C. bezziana Villeneuve and C. megacephala (Fabricius) were the dominant flies at outdoor carcasses. Meanwhile, the indoor carcasses was dominated by Lucilia spp.

Overwintering behaviour of the skipper fly (Diptera: Piophilidae) of forensic importance in Québec, Canada

2021 ◽  
pp. 1-9
Author(s):  
Julie-Éléonore Maisonhaute ◽  
Shari L. Forbes
Keyword(s):  
North America ◽  
Forensic Entomology ◽  
Laboratory Experiments ◽  
Field Observations ◽  
First Report ◽  
Fly Larvae

Abstract Laboratory experiments and field observations from August 2019 to April 2020 demonstrated that skipper flies (Diptera: Piophilidae) overwinter as larvae and likely present an obligatory winter diapause in Québec, Canada. Diapause was confirmed by the very few emergences of adults in the laboratory following collection from carrion at the end of summer and in fall, the migration of larvae deep in the soil, and the persistence of larvae inside carrion during the winter until the following spring when they became active again and initiated pupation (i.e., intra-puparial period). To our knowledge, our observations represent the first report of the overwintering of skipper fly larvae inside an animal carcass in North America. In addition, this winter diapause should be considered in forensic entomology when using the presence of skipper fly larvae on a body to estimate the time elapsed since death.

scholarly journals Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) development by landmark-based geometric morphometrics of cephalopharyngeal skeleton: a preliminary assessment for forensic entomology application

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Li-Xuan Sim ◽  
Raja M. Zuha
Keyword(s):  
Geometric Morphometrics ◽  
Forensic Entomology ◽  
Larval Growth ◽  
Age Groups ◽  
Developmental Time ◽  
Canonical Variate ◽  
Centroid Size ◽  
Geometric Morphometric ◽  
Chrysomya Megacephala ◽  
Cephalopharyngeal Skeleton

Abstract Background Considering the practicality of geometric morphometrics which could discriminate insect species, this application was extended to the analysis of blow fly larval growth based on cephalopharyngeal skeleton. In forensic entomology, cephalopharyngeal skeleton plays a crucial role in species identification but the morphometric information of this part is scarce. In this study, Chrysomya megacephala (Fabricius, 1794) was reared in two study replicates in natural conditions and samplings were conducted at fixed daily intervals. Cephalopharyngeal skeletons were removed from larvae and mounted on glass slides. Images were obtained from the specimens; digitized and geometric morphometric analysis on C. megacephala cephalopharyngeal skeletons was performed with MorphoJ software based on the ordination of five landmarks. The assessments of this analysis were based on centroid size measurements, visualization on the landmarks displacements, classification of the relative landmarks by using canonical variate analysis, and ontogenetic allometry determination. Findings Centroid size was strongly correlated with developmental time (p < 0.05) and significantly different between daily intervals (p < 0.05). Ontogenetic allometric effect based on multivariate regression on Procrustes coordinates and centroid size was significant (p < 0.0001), indicating that shape was influenced by growth (60.3%). Disposition occurred on all landmarks during development and was further discriminated based on age groups. Conclusions Other than discriminating between species, geometric morphometrics was found to be practical to visualize larval growth based on cephalopharyngeal skeletons which can be useful in forensic entomology.

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