Morphology and developmental rate of blowflies Chrysomya megacephala and Chrysomya rufifacies in Thailand: application in forensic entomology

2008 ◽  
Vol 102 (6) ◽  
pp. 1207-1216 ◽  
Author(s):  
Kom Sukontason ◽  
Somsak Piangjai ◽  
Sirisuda Siriwattanarungsee ◽  
Kabkaew L. Sukontason
2005 ◽  
Vol 42 (4) ◽  
pp. 702-704 ◽  
Author(s):  
Kabkaew L. Sukontason ◽  
Paitoon Narongchai ◽  
Duanghatai Sripakdee ◽  
Noppawan Boonchu ◽  
Tarinee Chaiwong ◽  
...  

2005 ◽  
Vol 42 (4) ◽  
pp. 702-704 ◽  
Author(s):  
Kabkaew L. Sukontason ◽  
Paitoon Narongchai ◽  
Duanghatai Sripakdee ◽  
Noppawan Boonchu ◽  
Tarinee Chaiwong ◽  
...  

Author(s):  
Y T B Bambaradeniya ◽  
W A I P Karunaratne ◽  
J K Tomberlin ◽  
P A Magni

Abstract Chrysomya rufifacies (Macquart), the hairy maggot blow fly, is of great importance for the field of forensic entomology due to its habit as an early colonizer of decomposing vertebrate remains and myiasis producer. Development studies on this species have been conducted in scattered regions of the world, using types of tissue from several species of animals as a rearing medium. Despite the commonality of C. rufifacies in Sri Lanka, developmental studies have never been performed in this region. As well, the effects of diet on development have not been tested. In the current study, C. rufifacies immatures were reared on skeletal muscle, liver, and heart from domestic swine, with flies from colonies maintained at 25 and 28°C. The minimum time needed to complete each stage at 25°C on liver (224.14 h) was fastest followed by skeletal muscle (249.33 h) and heart (251.64 h) respectively, whereas at 28°C, fly development was quickest on heart muscle (178.27 h) followed by liver (178.50 h) and skeletal muscle (186.17 h) respectively. A significant difference in total development time was determined for temperature, while the rearing medium was not significant. Temperature also showed a significant effect on the length and the width of the larvae, while the type of tissue statistically impacted only the width.


Author(s):  
Irwan Ismail ◽  
Yusuf Ridwan ◽  
Susi Soviana

This study aims to identify the diversity of flies that were caught and interacted with carcasses at the day and night. Each of the three rat carcasses (Rattus norvegicus) was placed outdoor and indoor within the campus of IPB Dramaga. The carcasses were put into a screen cage. Observation of flies was begun at 06:00 am and so on every four hours until the skeletal stage of the carcasses reached. The flies that come to the carcasses and into the screen cage were collected and identified. The decomposition of the indoor carcasses is slower than at outdoor, with the longer fresh stage. Meanwhile, post-decay stage and skeletal stage were achieved at the same time at each location. Nine spesies captured flies which are forensic indicator were Chrysomya megacephala (Fabricius), Chrysomya bezziana Villeneuve, Chrysomya rufifacies (Macquart), Chrysomya saffranea, Chrysomya spp, Lucilia spp, Sarcophaga spp, Calliphora spp and Musca domestica. C. bezziana Villeneuve and C. megacephala (Fabricius) were the dominant flies at outdoor carcasses. Meanwhile, the indoor carcasses was dominated by Lucilia spp.


Author(s):  
Laila A Al-Shuraym ◽  
Fahd A Al-Mekhlafi ◽  
Fahd Mohammed Abd Al Galil ◽  
Sadeq K Alhag ◽  
Lamya Ahmed Al-Keridis ◽  
...  

2019 ◽  
Vol 56 (6) ◽  
pp. 1623-1635 ◽  
Author(s):  
Sabrina Machado da Silva ◽  
Mauricio Osvaldo Moura

Abstract In forensic entomology, the minimum postmortem interval (PMImin) estimative is usually based on the oldest immature recovered from a local of death. The time spent by fly immatures in the intrapuparial period comprises more than 50% of their complete life cycles. An accurate estimate of the duration of this period will improve PMImin estimates. The blow fly Hemilucilia semidiaphana (Rondani 1850) was found in six criminal cases in the city of Curitiba. Even though there is data on the morphology of the larval instars and developmental rate of H. semidiaphana, the intrapuparial period has not been investigated. Here, we provide a detailed description of the intrapuparial morphological changes of H. semidiaphana, which might be useful to estimate minimum PMI. Samples of H. semidiaphana in the intrapuparial period were obtained from immatures reared on an artificial diet in incubators adjusted to 25°C or 20°C temperature regimes. Blow fly puparia of H. semidiaphana were fixed at intervals of 3 and 6 h until emergence of the adult. The external morphological traits of sampled immatures were analyzed using light microscopy. Our analysis of the intrapuparial period of H. semidiaphana provided 21 traits from which nine were age informative. These nine characteristics divide the developmental time (144 h at 25°C and 192 h at 20°C) into smaller sections. The developmental data provided, together with the time line allows a practical way to make interspecific comparisons as well as to estimate the age of H. semidiaphana based on the intrapuparial development.


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