scholarly journals Three-dimensional shape of the Golgi apparatus in different cell types: serial section scanning electron microscopy of the osmium-impregnated Golgi apparatus

Microscopy ◽  
2015 ◽  
Vol 65 (2) ◽  
pp. 145-157 ◽  
Author(s):  
Daisuke Koga ◽  
Satoshi Kusumi ◽  
Tatsuo Ushiki
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Golgi Apparatus ◽  
Serial Section ◽  
Three Dimensional ◽  
Cell Types ◽  
Dimensional Shape ◽  
Different Cell Types ◽  
Three Dimensional Shape ◽  
Scanning Electron

scholarly journals Three‐dimensional reconstructions of mouse circumvallate taste buds using serial blockface scanning electron microscopy: I. Cell types and the apical region of the taste bud

2019 ◽  
Vol 528 (5) ◽  
pp. 756-771 ◽  
Author(s):  
Ruibiao Yang ◽  
Yannick K. Dzowo ◽  
Courtney E. Wilson ◽  
Rae L. Russell ◽  
Grahame J. Kidd ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Three Dimensional ◽  
Cell Types ◽  
Taste Buds ◽  
Taste Bud ◽  
Apical Region ◽  
Scanning Electron

scholarly journals Coupled Broad Ion Beam–Scanning Electron Microscopy (BIB–SEM) for polishing and three dimensional (3D) serial section tomography (SST)

2020 ◽  
Vol 214 ◽  
pp. 112989
Author(s):  
Ali Gholinia ◽  
Matthew E. Curd ◽  
Etienne Bousser ◽  
Kevin Taylor ◽  
Thijs Hosman ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Serial Section ◽  
Ion Beam ◽  
Three Dimensional ◽  
Beam Scanning ◽  
Scanning Electron

scholarly journals Investigations on the postnatal development of the foliate papillae using light and scanning electron microscopy in the porcupine (Hystrix cristata)

2013 ◽  
Vol 58 (No. 6) ◽  
pp. 318-321 ◽  
Author(s):  
S. Yilmaz ◽  
A. Aydin ◽  
G. Dinc ◽  
B. Toprak ◽  
M. Karan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Types ◽  
Taste Buds ◽  
The Other ◽  
Average Depth ◽  
Hystrix Cristata ◽  
Basal Half ◽  
Different Cell Types ◽  
Scanning Electron

In this study SEM and light microscopy were used to investigate the structure of the foliate papillae in the porcupine. The foliate papillae consisted of about 10 or 11 clefts. The length of the foliate papillae averaged 2.79 mm and its width averaged 863 µm. Taste buds were located intraepithelial in the basal half of the papilla grooves (sulcus papillae). Every wall on each fold harboured from five to nine taste buds. There were two different cell types of taste buds: one stained light (epitheliocytus sensorius gustatorius), and the other dark (epitheliocytus sustentans). The length and width of the taste buds averaged 190.5 µm and 86 µm, respectively. The ratio of the length to the width of taste buds was 2.21. The average depth of the papilla groves was 1763 µm and its epithelial thickness was 235.5 µm. In scanning electron microscopy, the thickness of the epithelial cell borders was apparent at higher magnifications and there micro-ridges and micro-pits were apparent on the surfaces of these cells.  

Scanning and Transmission Microscopy of Nematocyst Batteries in Epitheliomuscular Cells of Hydra

1971 ◽  
Vol 29 ◽  
pp. 410-411 ◽  
Author(s):  
Jane A. Westfall ◽  
S. Yamataka ◽  
Paul D. Enos
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Osmium Tetroxide ◽  
External Surface ◽  
Three Dimensional ◽  
Surface Structures ◽  
Transmission Microscopy ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Scanning Electron

Scanning electron microscopy (SEM) provides three dimensional details of external surface structures and supplements ultrastructural information provided by transmission electron microscopy (TEM). Animals composed of watery jellylike tissues such as hydras and other coelenterates have not been considered suitable for SEM studies because of the difficulty in preserving such organisms in a normal state. This study demonstrates 1) the successful use of SEM on such tissue, and 2) the unique arrangement of batteries of nematocysts within large epitheliomuscular cells on tentacles of Hydra littoralis.Whole specimens of Hydra were prepared for SEM (Figs. 1 and 2) by the fix, freeze-dry, coat technique of Small and Màrszalek. The specimens were fixed in osmium tetroxide and mercuric chloride, freeze-dried in vacuo on a prechilled 1 Kg brass block, and coated with gold-palladium. Tissues for TEM (Figs. 3 and 4) were fixed in glutaraldehyde followed by osmium tetroxide. Scanning micrographs were taken on a Cambridge Stereoscan Mark II A microscope at 10 KV and transmission micrographs were taken on an RCA EMU 3G microscope (Fig. 3) or on a Hitachi HU 11B microscope (Fig. 4).

Scanning Electron Microscopy and Electron Microprobe Analysis of Malignant Cell Cultures

1968 ◽  
Vol 26 ◽  
pp. 164-165
Author(s):  
R. I. Johnsson-Hegyeli ◽  
A. F. Hegyeli ◽  
D. K. Landstrom ◽  
W. C. Lane
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Cultures ◽  
Electron Microprobe ◽  
Microprobe Analysis ◽  
Electron Microprobe Analysis ◽  
Three Dimensional ◽  
Malignant Cell ◽  
Interference Microscopy ◽  
Scanning Electron

Last year we reported on the use of reflected light interference microscopy (RLIM) for the direct color photography of the surfaces of living normal and malignant cell cultures without the use of replicas, fixatives, or stains. The surface topography of living cells was found to follow underlying cellular structures such as nuceloli, nuclear membranes, and cytoplasmic organelles, making possible the study of their three-dimensional relationships in time. The technique makes possible the direct examination of cells grown on opaque as well as transparent surfaces. The successful in situ electron microprobe analysis of the elemental composition and distribution within single tissue culture cells was also reported.This paper deals with the parallel and combined use of scanning electron microscopy (SEM) and the two previous techniques in a study of living and fixed cancer cells. All three studies can be carried out consecutively on the same experimental specimens without disturbing the cells or their structural relationships to each other and the surface on which they are grown. KB carcinoma cells were grown on glass coverslips in closed Leighto tubes as previously described. The cultures were photographed alive by means of RLIM, then fixed with a fixative modified from Sabatini, et al (1963).

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