Growth Regulation of Human Colon Adenocarcinoma-Derived Cells by Calcium, Vitamin D and Epidermal Growth Factor

1995 ◽  
Vol 125 (suppl_7) ◽  
pp. 2004S-2008S ◽  
Author(s):  
Heide S. Cross ◽  
Wolfgang Hulla ◽  
Wei-Min Tong ◽  
Meinrad Peterlik
Keyword(s):  
Vitamin D ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Growth Regulation ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Epidermal Growth ◽  
Human Colon Adenocarcinoma

Inhibition of sucrose-isomaltase expression by EGF in the human colon adenocarcinoma cells Caco-2

1991 ◽  
Vol 261 (6) ◽  
pp. C1173-C1183 ◽  
Author(s):  
H. S. Cross ◽  
A. Quaroni
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Marked Reduction ◽  
Transforming Growth Factor ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Mrna Levels ◽  
Proliferation And Differentiation ◽  
Epidermal Growth ◽  
Human Colon Adenocarcinoma

To investigate the role and mechanism of action of epidermal growth factor (EGF) in the intestinal epithelium, we have studied its influence on proliferation and differentiation of Caco-2 cells, a human colon adenocarcinoma cell line exhibiting several characteristics of adult small intestinal enterocytes. A clone of Caco-2 cells synthesizing minimal amounts of transforming growth factor-alpha (TGF-alpha)/epidermal growth factor (EGF)-like activity was used in these studies. Cells grown in the presence of 20-200 ng EGF/ml exhibited increased DNA synthesis and proliferation; formation of morphologically poorly differentiated multilayers was observed at 200 ng EGF/ml. At all concentrations tested EGF produced a significant and marked reduction in sucrase activity, whereas other brush-border enzymes (aminopeptidase N, alkaline phosphatase, dipeptidylpeptidase IV) were only marginally affected. EGF influenced sucrase expression at two different levels. At 20 ng/ml, it affected primarily sucrase-isomaltase processing in the endoplasmic reticulum and/or increased its degradation. At 200 ng EGF/ml, a significant and marked reduction in sucrase-isomaltase mRNA levels and biosynthesis was observed. These results demonstrated that EGF has important and selective effects on Caco-2 cell proliferation and differentiation and may affect different cellular activities depending on its concentration.

Renal synthesis of epidermal growth factor is unchanged by vitamin D deficiency

1990 ◽  
Vol 68 (6) ◽  
pp. 733-736 ◽  
Author(s):  
Paul R. Goodyer ◽  
Sharon Langshur ◽  
Jehane Fata
Keyword(s):  
Vitamin D ◽  
Parathyroid Hormone ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Vitamin D Deficiency ◽  
Thick Ascending Limb ◽  
Mrna Levels ◽  
Luminal Membrane ◽  
Calcium Reabsorption ◽  
Epidermal Growth

In mammalian kidney, epidermal growth factor (EGF) is produced as a small internal domain of an abundant high molecular weight peptide associated with the luminal membrane of the thick ascending limb of Henle's loop and distal convoluted tubule. At present, there is no evidence to indicate a mitogenic function for the EGF-containing molecule in kidney; consideration of its molecular structure suggests the possibility of a membrane-associated physiologic role. In this study, we examine regulation of renal EGF synthesis during induction of vitamin D deficiency in mice. Despite evidence of marked hyperparathyroidism, urinary excretion of EGF was equivalent in control (2.54 ± 0.72 μg/mg creatinine) and vitamin D deficient (2.13 ± 0.97 μg/mg creatinine) animals. Similarly, EGF mRNA levels in kidney were comparable in the two groups. These data indicate that parathyroid hormone has no effect on renal EGF regulation, although it is known to stimulate calcium reabsorption in distal nephron segments.Key words: epidermal growth factor, vitamin D, calcium, kidney, parathyroid hormone.

Sign in / Sign up

Export Citation Format

Share Document