scholarly journals Estimating chlorophyll content and photochemical yield of photosystem II (ΦPSII) using solar-induced chlorophyll fluorescence measurements at different growing stages of attached leaves

2015 ◽  
Vol 66 (18) ◽  
pp. 5595-5603 ◽  
Author(s):  
Bayaer Tubuxin ◽  
Parinaz Rahimzadeh-Bajgiran ◽  
Yusaku Ginnan ◽  
Fumiki Hosoi ◽  
Kenji Omasa
Keyword(s):  
Chlorophyll Fluorescence ◽  
Photosystem Ii ◽  
Chlorophyll Content ◽  
Fluorescence Measurements ◽  
Photochemical Yield

scholarly journals Limitation of photosynthetic processes in photosystem II in alpine mosses exposed to low temperatures: Response of chlorophyll fluorescence parameters

2018 ◽  
Vol 8 (2) ◽  
pp. 218-229 ◽  
Author(s):  
Yeray Folgar Cameán ◽  
Miloš Barták
Keyword(s):  
Chlorophyll Fluorescence ◽  
Low Temperature ◽  
Photosystem Ii ◽  
Temperature Stress ◽  
Low Temperature Stress ◽  
Ps Ii ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters ◽  
Fluorescence Measurements ◽  
Sphagnum Girgensohnii

In this study, we evaluated the effects of low and sub-zero temperature on the fast chlorophyll fluorescence transient (OJIP) and OJIP-derived parameters in 4 different mosses: Sphagnum girgensohnii, Polytrichum formosum, Hylocomium splendens and Pleurozium schreberi. The low temperature stress was applied on the mosses for 90 min. at 3 different temperatures (5°C, -1°C and -10°C). To investigate the effects of this stress on the functioning of photosystem II (PS II), the chlorophyll fluorescence measurements were taken at control temperature (22°C) and, after a 90 min. acclimation period, at each experimental temperature. The shape of OJIP curves and chlorophyll fluorescence parameters were found temperature-dependent in all the species. The mosses differed in their sensitivity to the stress but general trends in response to low temperature were similar. The results support the idea that S. girgensohnii is less resistant to low temperature stress than the other species. We were also interested in the K and L steps in OJIPs, representing different disorders caused by low temperature. The K-step was seen in P. formosum and P. schreberi and the L-step in H. splendens and S. girgensohnii.

Quantitative Mapping of Leaf Photosynthesis Using Chlorophyll Fluorescence Imaging

1995 ◽  
Vol 22 (2) ◽  
pp. 277 ◽  
Author(s):  
B Genty ◽  
S Meyer
Keyword(s):  
Chlorophyll Fluorescence ◽  
Electron Transport ◽  
Quantum Yield ◽  
Co2 Assimilation ◽  
Linear Electron ◽  
Leaf Photosynthesis ◽  
Heterogeneous Distribution ◽  
Fluorescence Measurements ◽  
Wide Range ◽  
Photochemical Yield

A method has been developed for routine, non-invasive monitoring of the topography of leaf photochemistry. The method uses video images of leaf chlorophyll fluorescence, taken during steady-state photosynthesis and during a transitory saturation of photochemistry, to construct, pixel by pixel, an image of the photochemical yield of photosystem II (PSII). The photochemical yield of PSII was estimated according to Genty et al. (1989) (Biochimica et Biophysica Acta 990, 87-92). The effectiveness of the method was shown by mapping the heterogeneous distribution of photosynthetic activity after treatment with either a herbicide (DCMU), abscisic acid, or during the course of the induction of photosynthesis. Leaf CO2 assimilation was simultaneously monitored under non- photorespiratory conditions to estimate the average quantum yield of linear electron transport. A unique proportional relationship was found between the mean photochemical yield of PSII calculated from images of the photochemical yield of PSII, and the average quantum yield of linear electron transport in three plant species exposed to a wide range of treatments or conditions. This new ability to quantitatively visualise leaf photochemistry provides a powerful tool to probe the spatial distribution of leaf photosynthesis. Possible errors in estimating the photochemical yield of PSII from mean fluorescence measurements are discussed.

scholarly journals Early Identification of Herbicide Modes of Action by the Use of Chlorophyll Fluorescence Measurements

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 529
Author(s):  
Sirous Hassannejad ◽  
Ramin Lotfi ◽  
Soheila P Ghafarbi ◽  
Abdallah Oukarroum ◽  
Amin Abbasi ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Stomatal Conductance ◽  
Chlorophyll Content ◽  
Fluorescence Induction ◽  
Leaf Temperature ◽  
Control Treatment ◽  
Chlorophyll Fluorescence Induction ◽  
Xanthium Strumarium ◽  
Chlorophyll Content And Fluorescence ◽  
Fluorescence Measurements

The effect of seven herbicides (U-46 Combi Fluid, Cruz, MR, Basagran Bromicide, Lumax, and Gramoxone) on Xanthium strumarium plants was studied. Chlorophyll content and fluorescence, leaf temperature, and stomatal conductance were evaluated at 12 h, 36 h, 60 h, and 84 h after herbicides application. U46 Combi Fluid, Cruz, and MR did not have a significant effect on chlorophyll fluorescence induction curves as compared to the control treatment. However, Basagran, Bromicide, Lumax, and Gramoxone showed significant changes in the shape of polyphasic fluorescence transients (OJIP transients). Variations in chlorophyll content index, leaf temperature, and stomatal conductance parameters were dependent on the type of applied herbicide. Our study revealed that the specific impact of the applied herbicides on the photosynthetic efficiency of plants is related to their chemical groups and their mechanism of action.

SO2 Injury in Intact Leaves, as Detected by Chlorophyll Fluorescence

1988 ◽  
Vol 43 (3-4) ◽  
pp. 269-274 ◽  
Author(s):  
Wolfgang Schmidt ◽  
Ulrich Schreiber ◽  
Wolfgang Urbach
Keyword(s):  
Chlorophyll Fluorescence ◽  
Photosystem Ii ◽  
Calvin Cycle ◽  
Photosynthetic Apparatus ◽  
Enzymatic Reactions ◽  
Decay Kinetics ◽  
Saturation Pulse ◽  
Induction Kinetics ◽  
Fluorescence Measurements

The effects of short-time fumigation (0-60 min) of intact spinach leaves with SO2 (2 ppm) on the photosynthetic apparatus were investigated. A rather high SO2 concentration was applied to monitor immediate effects on the fluorescence behaviour with the influence of repair processes or secondary types of damage being minimized. Three different types of in vivo chlorophyll fluorescence measurements were used: Rapid induction kinetics (Kautsky effect), slow induction kinetics with repetitive application of saturation pulses (saturation pulse method), and decay kinetics following a single turnover saturating flash. The slow induction kinetics with repetitive application of saturation pulses reacts in the most sensitive way indicating a primary damage at the level of the enzymatic reactions of the Calvin cycle. It is suggested that stromal acidification upon SO2 uptake interferes with light activation of Calvin cycle enzymes. With longer fumigation times also damage at the level of photosystem II becomes apparent: A decrease in variable fluorescence yield reflects a lowering of photosystem II quantum yield, and the slowing down of fluorescence relaxation kinetics reveals an effect on the secondary electron transport from Qᴀ to Qв. The detrimental effects of SO2 depend to a great extent on the application of light during fumigation. Besides a light requirement for SO2 uptake by stomata opening also the possibility of photoinhibitory damage is discussed. The susceptibility of leaves to photoinhibition may increase with a lowering of Calvin cycle activity by SO2.

Multiple Effects of Cadmium on the Photosynthetic Apparatus of Avicennia germinans L. as Probed by OJIP Chlorophyll Fluorescence Measurements

2007 ◽  
Vol 62 (3-4) ◽  
pp. 265-272 ◽  
Author(s):  
Daniel Gonzalez-Mendoza ◽  
Francisco Espadas y Gil ◽  
Jorge M. Santamaría ◽  
Omar Zapata-Perez
Keyword(s):  
Chlorophyll Fluorescence ◽  
Photosystem Ii ◽  
Water Splitting ◽  
Performance Index ◽  
Photosynthetic Apparatus ◽  
Reaction Centers ◽  
Avicennia Germinans ◽  
Fluorescence Measurements ◽  
Active Reaction ◽  
Primary Photochemistry

Abstract The toxic effects of cadmium on the photosynthetic apparatus of Avicennia germinans were evaluated by means of the chlorophyll fluorescence transient O-J-I-P. The chlorophyll fluorescence transients were recorded in vivo with high time resolution and analyzed according to the OJIP-test that can quantify the performance of photosystem II. Cadmium-treated plants showed a decrease in yield for primary photochemistry, TR0/ABS. The performance index of photosystem II (PSII), PIABS, decreased due to cadmium treatment. This performance index is the combination of the indexes of three independent parameters: (1) total number of active reaction centers per absorption (RC/ABS), (2) yield of primary photochemistry (TR0/ABS), and (3) efficiency with which a trapped exciton can move an electron into the electron transport chain (ET0/TR0). Additionally, the F0/Fv registered the highest sensitivity to the metal, thus indicating that the water-splitting apparatus of the oxidizing side of PSII is the primary site of action of cadmium. In summary, cadmium affects several targets of photosystem II. More specifically the main targets of cadmium, according to the OJIPtest, can be listed as a decrease in the number of active reaction centers and damage to the activity of the water-splitting complex.

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