Cell-Derived Microparticles in Blood Products from Thalassemic Blood Donors

2020 ◽  
Author(s):  
Egarit Noulsri ◽  
Surada Lerdwana ◽  
Duangdao Palasuwan ◽  
Attakorn Palasuwan
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Blood Donors ◽  
Blood Components ◽  
Blood Products ◽  
Packed Red Blood Cells ◽  
Thalassemia Trait ◽  
Routine Procedures

Abstract Objective To determine the number of cell-derived microparticles (MPs) in blood products obtained from donors who have thalassemia. Methods Packed red blood cells (PRBCs), plasma, and platelet concentrate (PC) were prepared according to routine procedures. We used flow cytometry to quantitate the concentration of MPs. Results The results of a comparison of MP levels in unprocessed whole blood showed that the concentration of all MPs in the donors without thalassemia trait (n = 255) was higher than in donors with thalassemia trait (n = 70). After processing, increased concentrations of MPs were documented in both groups. Among the blood components, PRBC showed higher platelet-derived MP concentrations in donors with thalassemia than in donors without thalassemia. However, PC showed higher concentrations of total MPs in donors without thalassemia than in donors with that condition. Conclusions Our results suggest little influence of thalassemia-trait status on changes in MP concentrations in blood components.

BLOOD PRODUCTS TRANSFUSION DURING 2012-13 IN PESHAWAR, PAKISTAN

2019 ◽  
Vol 16 (4) ◽  
pp. 105-108
Author(s):  
Rashid Azeem ◽  
Nadia Altaf ◽  
Syed Humayun Shah ◽  
Naeem Khattack ◽  
Muhammad Tariq Masood Khan ◽  
...  
Keyword(s):  
Health Care ◽  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Fresh Frozen Plasma ◽  
Health Care Facilities ◽  
Blood Products ◽  
Packed Red Blood Cells ◽  
Fresh Frozen ◽  
Frozen Plasma

Background: Blood products transfusion has been a major treatment modality especially in critical care settings. The objectives of this study were to determine frequency and distribution of blood products transfusion in public and private health care facilities during 2012-2013 in Peshawar, Pakistan. Materials & Methods: This cross-sectional, study was conducted in the Department of Pathology, Northwest School of Medicine, Peshawar, Pakistan from 1st January 2012 to 31st December 2013. Sample size was 2,04,942 blood products transfusion, selected through consecutive non probability technique. All allogeneic cases of transfusions in inpatient and emergency were included. A total of six public, one private and two stand-alone blood banks were enrolled into the study. Demographic variable were name of the health care facility, sex and age groups of donors. Research variables were type of blood products transfusion (whole blood, packed red blood cell, fresh frozen plasma, platelets). All variables being categorical were described as count and percentages. Data was analyzed using software SPSS version 23. Results: Out of 2,04,942 units, 1,33,212 (65%) were men and 71,730 (35%) women. Packed red blood cells were the most commonly used component with 80227 units (39.1%), whole blood 77655 units (37.8%), Fresh frozen plasma 35932 units (17.5%) and platelets 11128 units (5.6%). Blood products transfusion was 46927 units (22.89%) in 65 years. Conclusion: Modal group was men. Packed red blood cells were the most frequently transfused blood component in hospitals of Peshawar especially Lady reading hospital. Most common age group was 18-40 years. Whole blood still comprises a significant fraction of transfusions which is alarming.

Effect of an infusion device on the integrity of whole blood and packed red blood cells

1991 ◽  
Vol 48 (1) ◽  
pp. 92-97
Author(s):  
Kelly J. Burch ◽  
Stephanie J. Phelps ◽  
Thomas D. Constance
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Packed Red Blood Cells ◽  
Infusion Device

Administration of whole blood, packed red blood cells, and platelets using a multipurpose infusion pump

1991 ◽  
Vol 48 (9) ◽  
pp. 1970-1972
Author(s):  
Andy H. Strayer ◽  
David W. Henry ◽  
Allen Erenberg ◽  
Richard D. Leff
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Infusion Pump ◽  
Packed Red Blood Cells

Blood Shipping Containers: Whole Blood Versus Packed Red Blood Cells

1982 ◽  
Vol 147 (10) ◽  
pp. 809-817 ◽  
Author(s):  
Donald A. Smith ◽  
W. Patrick Monaghan
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Packed Red Blood Cells ◽  
Shipping Containers

scholarly journals Blood Component Therapy

2018 ◽  
Vol 9 (2) ◽  
pp. 142-147
Author(s):  
Shanaz Karim ◽  
Ehteshamul Hoque ◽  
Md Mazharul Hoque ◽  
Syeda Masooma Rahman ◽  
Kashfia Islam
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Fresh Frozen Plasma ◽  
Blood Component ◽  
Packed Red Blood Cells ◽  
Medical College ◽  
Blood Component Therapy ◽  
Fresh Frozen ◽  
Cellular Components

Transfusion medicine has undergone advancements since its initiation in the early 20th century. One of these was the discovery that blood can be divided into individual components and delivered separately. Today, blood transfusions nearly always consist of the ad-ministration of 1 or more components of blood. Whole blood transfusion is now limited to situations involving massive resuscitation (trauma ) The most familiar cellular components include packed red blood cells (PRBC), washed PRBC, leukoreduced PRBC and pooled or aphaeresis platelets. Plasma products such as FFP or cryoprecipitate, ant hemophilic factor (CRYO). The transfusion of red blood cells (RBCs), platelets, fresh-frozen plasma (FFP), and cryoprecipitate has the potential of improving clinical outcomes in perioperative and peripartum settings. These benefits include improved tissue oxygenation and decreased bleeding. However, transfusions are not without risks or costs. With the advent of blood component therapy, each unit of whole blood collected serves the specific needs of several, rather than a single patient.Anwer Khan Modern Medical College Journal Vol. 9, No. 2: Jul 2018, P 142-147

UK Helicopter Emergency Medical Services’ use of circulatory access devices, blood product transfusion and fluid warmers – a cross-sectional survey

Trauma ◽  
2018 ◽  
Vol 22 (1) ◽  
pp. 45-50
Author(s):  
Jonathan Morris ◽  
Simon Hughes
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Product ◽  
Central Venous Access ◽  
Venous Access ◽  
Blood Product Transfusion ◽  
Blood Products ◽  
Central Venous ◽  
Packed Red Blood Cells ◽  
Warming Devices

Introduction The pre-hospital environment provides significant challenges to clinicians who wish to rapidly administer warmed blood products and fluids to patients with haemorrhagic shock. Large-bore circulatory access is required with the use of devices that will successfully warm cold blood with minimal impact on flow rates. Until now, no information has been available that defines UK Helicopter Emergency Medical Services’ (HEMS) use of circulatory access and fluid warming devices, nor the recent adoption of pre-hospital blood product transfusion. Methods A survey was sent to all 22 UK HEMS asking which circulatory access devices crews have available, whether blood products are being transfused and if fluid warming devices are used as part of their resuscitations. Results All services responded. All UK HEMS use peripheral intravenous cannulae and intraosseous access. In addition, seven use central venous catheters and three use large-bore peripheral access (the Arrow Rapid Infusion Catheter®). Three services use landmark technique alone to gain central venous access, whereas four use a combination of landmark and ultrasound-guided techniques. Different sites for central venous access are used: subclavian (seven services), internal jugular (four) and femoral (four). Fourteen services carry pre-hospital blood products of which six transfuse packed red blood cells; four transfuse packed red blood cells and fresh frozen plasma; four transfuse packed red blood cells and lyophilised plasma. Eight services carry no pre-hospital blood products. Seventeen HEMS use fluid warmers; 13 use the Belmont® buddy lite™ and four use the QinFlow Warrior. Conclusion The use of a variety of policies and range of equipment has evolved across UK HEMS, demonstrating a lack of consensus on best practice. This is the first study to record a complete picture of current UK HEMS practice with regard to the use of circulatory access devices, fluid warmers and blood product administration.

Potassium Load in CPD-Preserved Whole Blood and Two Types of Packed Red Blood Cells

Transfusion ◽  
2003 ◽  
Vol 15 (2) ◽  
pp. 144-149 ◽  
Author(s):  
J. M. Michael ◽  
I. Dorner ◽  
D. Bruns ◽  
J. H. Ladenson ◽  
L. A. Sherman
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Packed Red Blood Cells

scholarly journals Indicators of Erythrocyte Damage after Microwave Warming of Packed Red Blood Cells

2003 ◽  
Vol 49 (5) ◽  
pp. 792-799 ◽  
Author(s):  
Jan Hirsch ◽  
Axel Menzebach ◽  
Ingeborg Dorothea Welters ◽  
Gerald Volker Dietrich ◽  
Norbert Katz ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Room Temperature ◽  
Antibody Binding ◽  
Cellular Damage ◽  
Substantial Part ◽  
Packed Red Blood Cells ◽  
Before And After ◽  
Cellular Markers

Abstract Background: Localized overheating of packed red blood cells (PRBCs) after microwave warming with consequent damage to erythrocytes has been reported. We therefore compared possible cellular markers of erythrocyte damage, as measured by flow cytometry, with laboratory indicators of hemolysis to evaluate the effects of microwave warming on PRBCs. Methods: PRBC samples were warmed to room temperature or to 37, 42, 47, 52, or 57 °C in a water bath. Flow cytometry was performed after fluorescein labeling using antibodies to spectrin, Ca2+-ATPase, and Na+-K+-ATPase. The forward-to-sideward scatter (FSC/SSC) ratio and antibody binding were evaluated. Plasma free hemoglobin (FHb) and α-hydroxybutyrate dehydrogenase (HBDH) were measured immediately after heating and after 48 h. In addition, all measurements were made before and after the heating of PRBCs to 35 °C by a microwave blood warmer. Results: Analysis of 15 000 erythrocytes showed a decrease in the FSC/SSC ratio and antibody binding above 47 °C [at 37 °C, median (SD) of 94.2 (7.4) with 0.07 (0.05)% fluorescein-positive; at 52 °C, median (SD) of 177.0 (19.0) with 18.5 (6.4)% positively gated; P <0.001]. FHb [room temperature, 0.3 (0.2) g/L] was increased 2-fold at 37 and 42 °C, 4-fold at 47 °C, and 25-fold at 52 °C. HBDH increased in parallel. Hemolysis markers showed an additional twofold increase 48 h after heating to 42 and 47 °C. Microwave heating to 35 °C did not produce significant changes of any marker. Conclusions: All markers of cellular damage were altered after heating to >47 °C, and a substantial part of hemolysis was delayed. The methodology can be used for future testing of other blood warming devices.

scholarly journals Influence of Prescribed Blood Products on the Incidence of Deep Vein Thrombosis and Pulmonary Embolism in Surgical Patients

2017 ◽  
Vol 23 (8) ◽  
pp. 938-942
Author(s):  
Alenka Premuš Marušič ◽  
Igor Locatelli ◽  
Aleš Mrhar ◽  
Martin Caprnda ◽  
Ludovit Gaspar ◽  
...  
Keyword(s):  
Deep Vein Thrombosis ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Surgical Patients ◽  
Blood Products ◽  
Control Groups ◽  
Packed Red Blood Cells ◽  
Vein Thrombosis ◽  
And Control ◽  
Deep Vein

Deep vein thrombosis (DVT) and pulmonary embolisms (PEs) are common complications after surgical procedures. The influence of prescribed blood products on the occurrence of DVT and PE was evaluated in postsurgical patients in this retrospective case–control study. The records of 286 surgical patients were analyzed: DVT (n = 52), PE (n = 92), and a control group (n = 142). The amounts of prescribed blood, blood products, and vitamin K were reviewed, together with appropriate prescribing of low-molecular-weight heparins. The influence of prescribed blood products on the occurrence of DVT or PE was analyzed using multinomial logistic regression. We demonstrated a significant difference between the test and control groups ( P < .05) in relation to receiving packed red blood cells. Treatment with red blood cells was associated with an increased risk of PE but not DVT. Patients who developed PE after surgery were hospitalized for longer (median 10 days) than patients with DVT (median 6 days). There was no difference between the test and control groups concerning treatment with fresh frozen plasma. Inadequate thromboprophylaxis significantly increased the likelihood of DVT. There is a connection between receiving packed red blood cells and occurrence of postoperative PE in surgical patients. Thus, patients receiving red blood cells should be monitored more closely after surgery, as they are more likely to develop PE postoperatively.

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