scholarly journals Nucleotide sequence of mouse genomic DNA coding for a brain specific small RNA

1988 ◽  
Vol 16 (1) ◽  
pp. 360-360
Author(s):  
Shunsuke Kobayashi ◽  
Kaijiro Anzai ◽  
Sataro Goto
Keyword(s):  
Nucleotide Sequence ◽  
Small Rna ◽  
Genomic Dna ◽  
Dna Coding ◽  
Mouse Genomic

scholarly journals Complete Genome Sequence of the Largest Known Flavi-Like Virus, Diaphorina citri flavi-like virus , a Novel Virus of the Asian Citrus Psyllid, Diaphorina citri

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Emilyn E. Matsumura ◽  
Luca Nerva ◽  
Jared C. Nigg ◽  
Bryce W. Falk ◽  
Shahideh Nouri
Keyword(s):  
Nucleotide Sequence ◽  
Small Rna ◽  
Complete Genome Sequence ◽  
Genome Organization ◽  
Complete Nucleotide Sequence ◽  
Transcriptome Sequencing ◽  
Asian Citrus Psyllid ◽  
Diaphorina Citri ◽  
Rt Pcr ◽  
Novel Virus

A novel flavi-like virus tentatively named Diaphorina citri flavi-like virus (DcFLV) was identified in field populations of Diaphorina citri through small RNA and transcriptome sequencing followed by reverse transcription (RT)-PCR. We report here the complete nucleotide sequence and genome organization of DcFLV, the largest flavi-like virus identified to date.

scholarly journals Nucleotide Sequence for the Genomic DNA Encoding an Anionic Peroxidase Gene from a Hybrid Poplar,Populus kitakamiensis

1993 ◽  
Vol 57 (1) ◽  
pp. 131-133 ◽  
Author(s):  
Shinya Kawai ◽  
Yasuo Matsumoto ◽  
Shinya Kajita ◽  
Keiko Yamada ◽  
Yoshihiro Katayama ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Genomic Dna ◽  
Hybrid Poplar ◽  
Dna Encoding ◽  
Anionic Peroxidase ◽  
Peroxidase Gene

A comparative hybridization analysis of yeast DNA with Paramecium parafusin- and different phosphoglucomutase-specific probes

2000 ◽  
Vol 78 (6) ◽  
pp. 683-690 ◽  
Author(s):  
Elzbieta Wyroba ◽  
Birgit H Satir
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nucleotide Sequence ◽  
Genomic Dna ◽  
Pcr Amplification ◽  
Molecular Size ◽  
Molecular Probes ◽  
Hybridization Analysis ◽  
Yeast Genome ◽  
Specific Primers ◽  
Genome Database

Molecular probes designed for the parafusin (PFUS), the Paramecium exocytic-sensitive phospho glyco protein, gave distinct hybridization patterns in Saccharomyces cerevisiae genomic DNA when compared with different phosphoglucomutase specific probes. These include two probes identical to segments of yeast phosphoglucomutase (PGM) genes 1 and 2. Neither of the PGM probes revealed the 7.4 and 5.9 kb fragments in Bgl II-cut yeast DNA digest detected with the 1.6 kb cloned PFUS cDNA and oligonucleotide constructed to the PFUS region (insertion 3 – I-3) not found in other species. PCR amplification with PFUS-specific primers generated yeast DNA-species of the predicted molecular size which hybridized to the I-3 probe. A search of the yeast genome database produced an unassigned nucleotide sequence that showed 55% identity to parafusin gene and 37% identity to PGM2 (the major isoform of yeast phosphoglucomutase) within the amplified region.Key words: parafusin, phosphoglucomutase, yeast, hybridization, PCR.

scholarly journals Structure of a murine alpha interferon pseudogene with a repetitive R-type sequence in the 3' flanking region.

1985 ◽  
Vol 5 (6) ◽  
pp. 1343-1348 ◽  
Author(s):  
D Le Roscouet ◽  
G Vodjdani ◽  
Y Lemaigre-Dubreuil ◽  
M G Tovey ◽  
M Latta ◽  
...  
Keyword(s):  
Amino Acids ◽  
Nucleotide Sequence ◽  
Genomic Library ◽  
Nucleotide Sequences ◽  
Alpha Interferon ◽  
Signal Sequences ◽  
Flanking Region ◽  
Flanking Regions ◽  
Mouse Genomic ◽  
Type Sequence

A murine alpha interferon pseudogene was identified in a mouse genomic library. The nucleotide sequence revealed several in-phase termination codons within the gene and repetitive oligonucleotides in the flanking regions. The nucleotide sequences and the amino acids of the peptide signal sequences were compared with known human alpha interferon genes and the pseudogene.

scholarly journals 102. Method Validation for Quantification of Recombinant Adeno-Associated Virus 5 in Mouse Genomic DNA Using Real-Time PCR

2016 ◽  
Vol 24 ◽  
pp. S44
Author(s):  
Hyun-Jung Kim ◽  
Sang-Jin Park ◽  
Sunyeong Lee ◽  
Ok-sun Kim ◽  
Su-Yeon Han ◽  
...  
Keyword(s):  
Real Time ◽  
Method Validation ◽  
Real Time Pcr ◽  
Genomic Dna ◽  
Adeno Associated Virus ◽  
Mouse Genomic

Divergent overlapping transcripts at the PET122 locus in Saccharomyces cerevisiae

1990 ◽  
Vol 10 (6) ◽  
pp. 3027-3035
Author(s):  
J D Ohmen ◽  
K A Burke ◽  
J E McEwen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nucleotide Sequence ◽  
Cytochrome C Oxidase ◽  
Genomic Dna ◽  
Transcription Unit ◽  
Open Reading Frames ◽  
Base Pairs ◽  
Opposite Strand ◽  
Overlapping Transcripts ◽  
Reading Frames

PET122 is one of three nuclear genes specifically required for translation of the mitochondrial mRNA for cytochrome c oxidase subunit III in Saccharomyces cerevisiae. The nucleotide sequence of 2,862 base pairs (bp) of yeast genomic DNA encompassing the PET122 locus shows very close spacing between the PET122 gene (254 codons) and two unidentified open reading frames, termed ORF2 and ORF3. ORF2 is encoded by the same strand of DNA as PET122 and is located 53 bp downstream of PET122, while ORF3 is encoded on the opposite strand and is located 215 bp upstream of PET122. Five transcripts, with sizes of 2.9, 2.3, 2.1, 1.5, and 1.4 kilobases (kb), are produced from this locus. The 2.1- and 1.4-kb transcripts encode ORF3, the 1.5-kb transcript encodes ORF2, and the 2.9- and 2.3-kb transcripts encode PET122. A particularly interesting feature of the ORF3-PET122-ORF2 transcription unit is a 535-base overlap between the 2.3-kb PET122 transcript produced from one strand and a 2.1-kb ORF3 transcript produced from the opposite strand. Similarly, the 2.9-kb PET122 transcript overlaps the 2.1-kb ORF3 transcript by more than 900 bases and the 1.5-kb ORF3 transcript by at least 200 bases. Hence, these pairs of transcripts are antisense to one another and have the potential to regulate, in an interdependent fashion, the posttranscriptional expression of ORF3 and PET122.

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