987Carbapenem-resistant Klebsiella pneumoniae (CRKp) in Children. Clinical and Epidemiological Characteristics from Infected and Colonized Inpatients in a Tertiary care Hospital in Medellín, Colombia

The recent emergence of multidrug-resistant (MDR) Klebsiella pneumoniae with hypervirulent traits causing severe infections and considerable mortality is a global cause for concern. The challenges posed by these hypermucoviscous strains of K. pneumoniae with regard to their optimal treatment, management, and control policies are yet to be answered. We studied a series of extensively drug-resistant (XDR) and hypervirulent K. pneumoniae ST5235 isolates with resistance to carbapenems and polymyxins causing neonatal sepsis in a tertiary care hospital in India. A total of 9 K. pneumoniae isolates from 9 cases of neonatal sepsis were studied with respect to their clinical relevance, antimicrobial susceptibility profile, presence of extended spectrum β lactamase (ESBL) production, and responsible genes, carbapenemases (classes A, B, and D), and aminoglycoside-resistant genes. Hypervirulence genes encoding hypermucoid nature, iron uptake, and siderophores were detected by multiplex PCR. The plasmid profile was studied by replicon typing. Isolates were typed by multilocus sequence typing (MLST) and enterobacterial repetitive intergenic consensus (ERIC) PCR to study the sequence types (STs) and clonal relation, respectively. The neonates in the studied cases had history of pre-maturity or low birth weight with maternal complications. All the cases were empirically treated with piperacillin–tazobactam and amikacin followed by imipenem/meropenem and vancomycin and polymyxin B as a last resort. However, all the neonates finally succumbed to the condition (100%). The studied isolates were XDR including resistance to polymyxins harboring multiple ESBL genes and carbapenemase genes (blaNDM and blaOXA−48). Hypervirulence genes were present in various combinations with rmpA/A2 genes present in all the isolates. IncFI plasmids were detected in these isolates. All belonged to ST5235. In ERIC PCR, 6 different clusters were seen. The study highlighted the emergence and burden of XDR hypervirulent isolates of K. pneumoniae causing neonatal sepsis in a tertiary care hospital.

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The clinical study of dermatophytosis in a tertiary care hospital of konkan region, Maharashtra

IP Indian Journal of Clinical and Experimental Dermatology ◽

10.18231/j.ijced.2021.059 ◽

2021 ◽

Vol 7 (4) ◽

pp. 315-319

Author(s):

U Ketan Raju ◽

Hanamant G Bobade ◽

Anil Mishra

Keyword(s):

Tertiary Care Hospital ◽

Tertiary Care ◽

Hair Shaft ◽

Care Hospital ◽

Common Site ◽

Tinea Cruris ◽

Nail Changes ◽

Male Preponderance ◽

History Of ◽

Epidemiological Characteristics

Dermatophytes are aerobic fungi that produce proteases that digest keratin and allows colonization, invasion and infection of the stratum corneum of the skin, the hair shaft, and the nail. This study was an attempt on the part of the author to find out the incidence and various clinico-epidemiological characteristics of Dermatophytosis in skin OPD of Tertiary care Hospital, B.K.L.W.R.M.C., Dervan. The study was carried on 2444 patients of Dermatophytosis attending the OPD, out of which 49.79% of the cases were Tinea Cruris (TC). The age group of 11-20 years showed maximum prevalence of Dermatophytosis (22.17%). 58% of the patients had diffuse lesions. Body involvement was seen in 87% of cases. Itching was the most common condition associated with Dermatophytosis. 35% of the patients reported with family history of Tinea Cruris.Circumscribed variety of TC was found to be most common variant mostly observed in 2and 4 decade, with a male preponderance. Groin was the most common site with multiple lesions. Nail changes was most commonly associated with TC.

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Antimicrobial resistance pattern of Klebsiella pneumoniae isolated from various clinical samples in a tertiary care hospital

Indian Journal of Medical Microbiology ◽

10.1016/j.ijmmb.2021.08.016 ◽

2021 ◽

Vol 39 ◽

pp. S4

Author(s):

S. Meera Rajeev ◽

Madhumati Patil

Keyword(s):

Antimicrobial Resistance ◽

Klebsiella Pneumoniae ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Clinical Samples ◽

Resistance Pattern ◽

Care Hospital

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Secular trends in Klebsiella pneumoniae isolated in a tertiary-care hospital: increasing prevalence and accelerated decline in antimicrobial susceptibility

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/0037-8682-0072-2016 ◽

2016 ◽

Vol 49 (2) ◽

pp. 177-182 ◽

Cited By ~ 2

Author(s):

Rodrigo de Carvalho Santana ◽

Gilberto Gambero Gaspar ◽

Fernando Crivelenti Vilar ◽

Fernando Bellissimo-Rodrigues ◽

Roberto Martinez

Keyword(s):

Klebsiella Pneumoniae ◽

Antimicrobial Susceptibility ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Secular Trends ◽

Care Hospital

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Virulence determinant and extended spectrum beta-lactamase production in Klebsiella pneumoniae isolated from a tertiary care hospital, South India

Journal of Laboratory Physicians ◽

10.4103/jlp.jlp_30_17 ◽

2018 ◽

Vol 10 (02) ◽

pp. 155-161

Author(s):

Lathamani Kotekani ◽

Subbannayya Kotigadde

Keyword(s):

Klebsiella Pneumoniae ◽

South India ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Generation Cephalosporin ◽

Epidemiological Surveillance ◽

Serum Resistance ◽

Care Hospital ◽

Extended Spectrum Beta Lactamase ◽

Virulence Determinant

ABSTRACT BACKGROUND: Klebsiella pneumoniae on these days show greater resistance towards newer generation cephalosporin. The present study made an effort to show the relevance between ESBL producing K. pneumoniae and virulence determinant in respect to serum resistance and K1, K2 antigens at a tertiary care hospital in South India. MATERIAL AND METHODS: A total of 520 consecutive, non-duplicate isolates of K. pneumoniae were recovered from various clinical specimens such as Urine (n = 360), sputum (n = 107), pus (n = 25), blood (n = 6) and other miscellaneous specimens (n=22) obtained from both out patients and in patients between June 2012 to July 2016 were included in the study. RESULTS: Polymerase Chain Reaction to detect bla genes in 62 isolates phenotypically identified as ESBL producers were successful in 58 (93.54%) isolates. Of the 13 ESBL producing hypermucoviscous Klebsiella pneumoniae (hvKP) strains, 6 of them were showing the amplicons for gene's coding for K1 antigens. CONCLUSION: The study provides further authentication of the global sporadic of bla CTX-M and the relevance between K antigens and serum resistance with ESBL production in our place. As there is no much study available, it also highlights the need for further study of their epidemiological surveillance.

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