Transport of Sugars and Amino Acids in BacteriaXI. Mechanism of Energy Coupling Reaction for the Concentrative Uptake of Proline by Escherichia coli Membrane Vesicles*

1974 ◽  
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Coupling Reaction ◽  
Membrane Vesicles ◽  
Energy Coupling

scholarly journals Energy Coupling in Membrane Vesicles of Escherichia coli

1974 ◽  
Vol 249 (9) ◽  
pp. 2939-2945
Author(s):  
Hajime Hirata ◽  
Karlheinz Altendorf ◽  
Franklin M. Harold
Keyword(s):  
Escherichia Coli ◽  
Membrane Vesicles ◽  
Energy Coupling

The action of tributyltin chloride on the uptake of proline and glutamine by intact cells of Escherichia coli

1979 ◽  
Vol 57 (12) ◽  
pp. 1376-1383 ◽  
Author(s):  
A. P. Singh ◽  
P. D. Bragg
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Lipoic Acid ◽  
Membrane Vesicles ◽  
Electrochemical Gradient ◽  
Intact Cells ◽  
Iodide Ions ◽  
Tributyltin Chloride ◽  
Anion Exchange Reaction ◽  
Glutamine Uptake

Tributyltin chloride inhibits growth and uptake of glutamine and proline into intact cells of Escherichia coli. It causes efflux of the accumulated amino acids. A pH gradient generated in intact cells and everted membrane vesicles is dissipated by this compound. These effects do not require lipoic acid but are dependent on the presence of chloride, bromide, or iodide ions. We conclude that tributyltin chloride can catalyse a transmembrane OH−–anion exchange reaction and that this is its mode of inhibition of the uptake of these amino acids. The response of proline and glutamine uptake to the inhibitor is similar and is consistent with the transport of both amino acids requiring an electrochemical gradient of protons.

Coupling of Glycine and Alanine Transport to Respiration in Cells of Escherichia coli

1975 ◽  
Vol 53 (3) ◽  
pp. 262-268 ◽  
Author(s):  
G. D. Sprott ◽  
K. Dimock ◽  
W. G. Martin ◽  
Henry Schneider
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
High Intensity ◽  
Aromatic Amino Acids ◽  
Energy Coupling ◽  
Anoxic Conditions ◽  
Violet Light ◽  
Alanine Transport ◽  
Complete Dependence ◽  
In Cells

Energy coupling for uptake of glycine and alanine in glycerol grown cells of Escherichia coli differs from that of the aromatic amino acids. Respiration and uptake of glycine and alanine show similar inactivations in cells exposed to high intensity violet light or to various concentrations of cyanide. In contrast, uptake of phenylalanine, tyrosine, and tryptophan is resistant to effects of light or cyanide. Anoxia largely inhibits uptake of glycine and alanine while that of the aromatic amino acids is only partially affected. Furthermore, ferricyanide (but not ferrocyanide) completely restores active uptake of aromatic amino acids under anoxic conditions but is without effect on glycine and alanine uptake. Adenosine 5′-triphosphate (ATP) concentration does not increase in anoxic cells exposed to ferricyanide, indicating that ATP cannot be responsible for this restoration. The data suggest that glycine and alanine represent amino acids whose transport shows a complete dependence on energy derived from respiration, while the energy for transport of the aromatic amino acids may be obtained from other sources.

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