scholarly journals Photocrosslinking silver nanoparticles–aloe vera–silk fibroin composite hydrogel for treatment of full-thickness cutaneous wounds

2021 ◽  
Vol 8 (6) ◽  
Author(s):  
Yangkun Liu ◽  
JinChuan Fan ◽  
MingQi Lv ◽  
Kepeng She ◽  
Jiale Sun ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Silver Nanoparticles ◽  
Silk Fibroin ◽  
Aloe Vera ◽  
Antibacterial Properties ◽  
Composite Hydrogel ◽  
Wound Dressings ◽  
Full Thickness ◽  
Cutaneous Wounds

Abstract Damage to the skin causes physiological and functional issues. The most effective treatment approach is the use of wound dressings. Silk fibroin (SF) is a promising candidate biomaterial for regulating wound healing; however, its antibacterial properties and biological activity must be further improved. In this study, a photocrosslinking hydrogel was developed to treat full-thickness cutaneous wounds. The composite hydrogel (Ag–AV–SF hydrogel) was prepared by introducing the silver nanoparticles (AgNPs) and aloe vera (AV) as the modifiers. In vitro study exhibited great antibacterial ability, biocompatibility and cell-proliferation and -migration-promoting capacities. It also showed the pH-response releasing properties which release more AgNPs in a simulated chronic infection environment. The healing effect evaluation in vivo showed the healing-promoting ability of the Ag–AV–SF hydrogel was stronger than the single-modifiers groups, and the healing rate of it reached 97.02% on Day 21, higher than the commercial wound dressing, silver sulfadiazine (SS) cream on sale. Additionally, the histological and protein expression results showed that the Ag–AV–SF hydrogel has a greater effect on the pro-healing regenerative phenotype with M2 macrophages at the early stage, reconstructing the blood vessels networks and inhibiting the formation of scars. In summary, the Ag–AV–SF hydrogel developed in this study had good physical properties, overwhelming antibacterial properties, satisfactory biocompatibility and significantly promoting effect on cell proliferation, migration and wound healing. Overall, our results suggest that the Ag–AV–SF hydrogel we developed has great potential for improving the wound healing in clinical treatment.

scholarly journals Silk derived formulations for accelerated wound healing in diabetic mice

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10232
Author(s):  
Muniba Tariq ◽  
Hafiz Muhammad Tahir ◽  
Samima Asad Butt ◽  
Shaukat Ali ◽  
Asma Bashir Ahmad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Chronic Wounds ◽  
Histological Analysis ◽  
Healing Process ◽  
Aloe Vera ◽  
Wound Contraction ◽  
Wound Dressings ◽  
Control Group ◽  
Diabetic Mice

Background The present study aimed to prepare effective silk derived formulations in combination with plant extract (Aloe vera gel) to speed up the wound healing process in diabetic mice. Methods Diabetes was induced in albino mice by using alloxan monohydrate. After successful induction of diabetes in mice, excision wounds were created via biopsy puncture (6 mm). Wound healing effect of silk sericin (5%) and silk fibroin (5%) individually and in combination with 5% Aloe vera gel was evaluated by determining the percent wound contraction, healing time and histological analysis. Results The results indicated that the best biocompatible silk combination was of 5% silk fibroin and 5% Aloe vera gel in which wounds were healed in 13 days with wound contraction: 98.33 ± 0.80%. In contrast, the wound of the control group (polyfax) healed in 19 day shaving 98.5 ± 0.67% contraction. Histological analysis revealed that the wounds which were treated with silk formulations exhibited an increased growth of blood vessels, collagen fibers, and much reduced inflammation. Conclusion It can be concluded that a combination of Bombyx mori silk and Aloe vera gel is a natural biomaterial that can be utilized in wound dressings and to prepare more innovative silk based formulations for speedy recovery of chronic wounds.

Efficiency Improvement of Gelatin-Based Wound Dressings by Silk Fibroin and Chitosan

2020 ◽  
Vol 995 ◽  
pp. 97-102
Author(s):  
Wassanai Wattanutchariya ◽  
Kittiya Thunsiri ◽  
Suwichchaya Puntawang
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Silk Fibroin ◽  
Healing Process ◽  
Fibrous Tissue ◽  
Fibroblast Cell ◽  
The Body ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Moisture Condition

Wound healing is a natural process of human body. When the wound size exceeds the critical point for naturally body healing, the fibrous tissue will play their parts and created a scar. Therefore, extra treatment has been added to eliminate the body limitations. Currently, there are a lot of commercial bioactive wound healing and dressing due to its physiological and biological abilities. In wound healing process, high moisture condition is also required. In order to accelerate the wound healing process, Tissue engineering (TE) is recommended. The increasing of cell proliferation by TE will be increased the chance for wound healing acceleration. In this study, the combination of Gelatin (Gel), Chitosan (CS) and Silk Fibroin (SF) were varied mixed in 10 ratios and fabricated the structure by lyophilisation technique. The elastic ability, biodegradability, structure and pore morphology, porosity, swelling ability, and biotoxicity were observed in each ratio. Gel provided highest elastic ability and biodegradability. The addition of SF and CS in Gel decreased biodegradation rate and activate fibroblast cell proliferation. Therefore, CS and SF could increase efficiency of gelatin-base wound dressings for a variety of utilization.

Generation of Nano-pores in Silk Fibroin Films Using Silk Nanoparticles for Full-Thickness Wound Healing

2021 ◽  
Vol 22 (2) ◽  
pp. 546-556
Author(s):  
Jian Liu ◽  
Ran Huang ◽  
Gang Li ◽  
David L. Kaplan ◽  
Zhaozhu Zheng ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Full Thickness ◽  
Full Thickness Wound

A moisturizing chitosan-silk fibroin dressing with silver nanoparticles-adsorbed exosomes for repairing infected wounds

2020 ◽  
Vol 8 (32) ◽  
pp. 7197-7212 ◽  
Author(s):  
Zhiyong Qian ◽  
Yating Bai ◽  
Jin Zhou ◽  
Linhao Li ◽  
Jing Na ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Silver Nanoparticles ◽  
Silk Fibroin ◽  
Broad Spectrum ◽  
Electrolyte Balance ◽  
Infected Wounds ◽  
Multifunctional Properties

The CTS-SF/SA/Ag-Exo dressing possessed multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.

scholarly journals Antibacterial-Integrated Collagen Wound Dressing for Diabetes-Related Foot Ulcers: An Evidence-Based Review of Clinical Studies

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2168
Author(s):  
Ibrahim N. Amirrah ◽  
Mohd Farhanulhakim Mohd Razip Wee ◽  
Yasuhiko Tabata ◽  
Ruszymah Bt Hj Idrus ◽  
Abid Nordin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Clinical Trials ◽  
Clinical Studies ◽  
Wound Dressing ◽  
Chronic Wounds ◽  
Gentian Violet ◽  
Antibacterial Properties ◽  
Small Sample ◽  
Wound Dressings ◽  
Sample Sizes

Diabetic foot ulcer (DFU) is a chronic wound frequently delayed from severe infection. Wound dressing provides an essential barrier between the ulcer and the external environment. This review aimed to analyse the effectiveness of antibacterial collagen-based dressing for DFU treatment in a clinical setting. An electronic search in four databases, namely, Scopus, PubMed, Ovid MEDLINE(R), and ISI Web of Science, was performed to obtain relevant articles published within the last ten years. The published studies were included if they reported evidence of (1) collagen-based antibacterial dressing or (2) wound healing for diabetic ulcers, and (3) were written in English. Both randomised and non-randomised clinical trials were included. The search for relevant clinical studies (n) identified eight related references discussing the effectiveness of collagen-based antibacterial wound dressings for DFU comprising collagen impregnated with polyhexamethylene biguanide (n = 2), gentamicin (n = 3), combined-cellulose and silver (n = 1), gentian violet/methylene blue mixed (n = 1), and silver (n = 1). The clinical data were limited by small sample sizes and multiple aetiologies of chronic wounds. The evidence was not robust enough for a conclusive statement, although most of the studies reported positive outcomes for the use of collagen dressings loaded with antibacterial properties for DFU wound healing. This study emphasises the importance of having standardised clinical trials, larger sample sizes, and accurate reporting for reliable statistical evidence confirming DFU treatment efficiency.

scholarly journals The Effect of Aloe vera (Linn) On Cutaneous Wound Healing and Wound Contraction Rate in Adult Rabbits

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Martha Orendu Attah ◽  
Tonye Watson Jacks ◽  
Attah Jacob ◽  
Otong Eduitem ◽  
Barnabas John
Keyword(s):  
Wound Healing ◽  
Aloe Vera ◽  
Wound Contraction ◽  
Control Group ◽  
Cutaneous Wound Healing ◽  
Contraction Rate ◽  
Wound Area ◽  
Cutaneous Wound ◽  
Cutaneous Wounds ◽  
Aloe Vera Gel

Background: In the present research study, the rate of cutaneous wound healing and contraction rate in healthy rabbits using Aloe vera pulp was studied.Methods: Ten healthy rabbits were used for the study. They were divided into two groups consisting of five rabbits each. Cutaneous wounds were made on the lumbar region of each rabbit using a template which ensured that the wounds were of the same size in all the rabbits. 5ml of Aloe vera gel was applied to the wounds of the animals in the test group, while nothing was applied to the wound area of the animals in the control group. The wound area in each group was measured for a period of 21 days, using a venire caliper and tracing paper which was used to trace the wound area. Tissue samples were removed from the wound area in both experimental and control groups and subjected to routine histological analysis, also, morphometric analysis was performed.Results: The rate of wound contraction and mean centripetal contraction was calculated in both groups and graphically represented using Microsoft Excel. The results showed that animals who were treated with Aloe vera gel had a greater wound contraction rate, as well as rapid wound closure. The micrographs showed a thicker epithelial layer, with thinner collagen fibers in the dermis of experimental animals compared to the control group. There was also an abundant capillary bed at the dermal-epidermal junction in the experimental group, compared to the control group.Conclusion:  Aloe vera may increase the rate of wound healing by accelerating epithelial migration, and may also play a role in neo-vascularization of the newly healed area.Keywords: Aloe vera, Cutaneous Wounds, Rabbits, Wound Contraction, Wound Healing

scholarly journals Antibacterial and Wound Healing Activities of Silver Nanoparticles Embedded in Cellulose Compared to Other Polysaccharides and Protein Polymers

2021 ◽  
Author(s):  
Mehran Alavi ◽  
Rajender S. Varma
Keyword(s):  
Thermal Stability ◽  
Wound Healing ◽  
Silver Nanoparticles ◽  
Chronic Wounds ◽  
Colloidal Solution ◽  
Antibacterial Activities ◽  
Wound Dressings ◽  
Future Perspective ◽  
Natural Polymers ◽  
Therapeutic Properties

Abstract The aggregation of silver nanoparticles (AgNPs) in colloidal solution and the oxidative cytotoxicity towards human cells are two major hindrances for their thriving medicinal applications. Their incorporation in natural polymers such as cellulose, chitosan, alginate, collagen, gelatin, silk fibroin, carrageenan, hyaluronic acid, keratin and starch may be an alluring alternative strategy to sidestep these complications and attaining the advantageous wound dressings. Biocompatibility, bioavailability, biodegradability, and inherent therapeutic properties known for theses polymers, would accelerate the healing of infected chronic wounds. However, the low thermal stability, mechanical strength, rapid biodegradation, and weak washing resistance properties are some of the limitations for these polymers. Herein, recent advances, present challenges and future perspective for AgNPs incorporated nanocomposites (NCs) are discussed to realize ideal antibacterial activities by exploiting the abundant natural biopolymers.

Metal doped calcium silicate biomaterial for skin tissue regeneration in vitro

2020 ◽  
pp. 088532822096260
Author(s):  
K Mohamed Abudhahir ◽  
R Murugesan ◽  
R Vijayashree ◽  
N Selvamurugan ◽  
Tze-Wen Chung ◽  
...  
Keyword(s):  
Wound Healing ◽  
Calcium Silicate ◽  
Soft Tissues ◽  
Healing Process ◽  
Antibacterial Properties ◽  
Clinical Applications ◽  
Wound Dressings ◽  
Skin Tissue ◽  
Wound Healing Process

This study spots light on combined Wound healing process conjoining blood coagulation, inflammation reduction, proliferation and remodeling of the cells. The objective is to overcome the drawbacks of conventional clinically applied wound dressings such as poor rigidity, porosity, mechanical potency and bactericidal activity. As nosocomial infection is a very common condition at the wound site, bio-adhesive materials with intrinsic antibacterial properties are used in clinical applications. Considering the provenability of Wollastonite [Calcium silicate (CaSiO3)] to regenerate the soft tissues by inducing vascularization and regeneration of fibroblast cells And the antibacterial potentiality of zinc in clinical applications, the present study focuses on synthesis of Zn-Ws particles and evaluation of its antimicrobial and wound healing potentialities towards skin tissue engineering applications. The compositional characterization by EDAS and FT-IR spectral analysis have substantiated the presence of major elements and corresponding band stretching associated with the synthesized particles whereas the particles morphology by SEM images have shown the size of the Ws and Zn-Ws to be 370 nm and 530 nm respectively. From the in vitro studies, skin regenerative potential of Zn-Ws was determined on promoting fibroblast cell (NIH3T3) proliferation by providing better adhesiveness, biocompatibility and cytocompatibility. The antibacterial property of Zn-Ws evaluation by minimum inhibitory concentration (MIC) and zone of inhibition (ZOI) methods against clinical isolates of Gram +Ve and Gram –Ve bacterial strains have confirmed that the addition of Zn has diminished the bacterial growth and also helped in degrading the bacterial biofilms. Thus it is summed up that the process of wound healing is expected to occur with reduced risk of post-injury infections by the presence of zinc-doping on wollastonite for skin tissue application.

Sign in / Sign up

Export Citation Format

Share Document