scholarly journals Silk derived formulations for accelerated wound healing in diabetic mice

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10232
Author(s):  
Muniba Tariq ◽  
Hafiz Muhammad Tahir ◽  
Samima Asad Butt ◽  
Shaukat Ali ◽  
Asma Bashir Ahmad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Chronic Wounds ◽  
Histological Analysis ◽  
Healing Process ◽  
Aloe Vera ◽  
Wound Contraction ◽  
Wound Dressings ◽  
Control Group ◽  
Diabetic Mice

Background The present study aimed to prepare effective silk derived formulations in combination with plant extract (Aloe vera gel) to speed up the wound healing process in diabetic mice. Methods Diabetes was induced in albino mice by using alloxan monohydrate. After successful induction of diabetes in mice, excision wounds were created via biopsy puncture (6 mm). Wound healing effect of silk sericin (5%) and silk fibroin (5%) individually and in combination with 5% Aloe vera gel was evaluated by determining the percent wound contraction, healing time and histological analysis. Results The results indicated that the best biocompatible silk combination was of 5% silk fibroin and 5% Aloe vera gel in which wounds were healed in 13 days with wound contraction: 98.33 ± 0.80%. In contrast, the wound of the control group (polyfax) healed in 19 day shaving 98.5 ± 0.67% contraction. Histological analysis revealed that the wounds which were treated with silk formulations exhibited an increased growth of blood vessels, collagen fibers, and much reduced inflammation. Conclusion It can be concluded that a combination of Bombyx mori silk and Aloe vera gel is a natural biomaterial that can be utilized in wound dressings and to prepare more innovative silk based formulations for speedy recovery of chronic wounds.

scholarly journals Efficacy of negative pressure wound therapy using suction drain in the management of chronic wounds

2018 ◽  
Vol 5 (6) ◽  
pp. 2256 ◽  
Author(s):  
Mohammed Kashif Imran ◽  
Mohan Kumar K. ◽  
Sreeramulu P. N. ◽  
Bhaskaran . ◽  
Krishna Prasad K. ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hospital Stay ◽  
Negative Pressure ◽  
Chronic Wounds ◽  
Adult Population ◽  
Healing Process ◽  
Wound Dressings ◽  
Control Group ◽  
Wound Healing Process ◽  
Topical Negative Pressure

Background: Chronic non-healing wounds are one of the major burden to the patients in the present era and covers about 1% of adult population and 3.6% of the population greater than 65 years. Chronic leg wounds are the common cause of morbidity and its prevalence in the community range from 1.9 to 13.1%. Indian studies show the prevalence of chronic wounds of around 4.5/1000 population. Recent studies have shown that application of a sub atmospheric pressure in a controlled manner to the wound site helps significantly in wound healing. Aim of the present study is to assess the efficacy of topical negative pressure moist wound dressings in wound healing process in chronic wounds and to prove that negative pressure dressings can be used as a much better treatment option than conventional saline dressings in the management of chronic wounds.Methods: After 10 days, in study group the mean rate of granulation tissue formation, mean graft, mean hospital stay was 81.7%,80.6%, 34.7 days respectively and the same in control group it is 41.9%. ,60.45%, 58.60 days respectively.Results: To conclude, topical negative pressure dressing’s help in faster healing of chronic wounds and better graft take up and reduces hospital stay of these patients.Conclusions: The database of our retrospective study regarding age and sex incidence, clinicopathological features and therapeutic outcome was comparable to other studies in various literatures.

scholarly journals Polymer-Based Scaffolds Loaded with Aloe vera Extract for the Treatment of Wounds

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 961
Author(s):  
Sibusiso Alven ◽  
Vuyolwethu Khwaza ◽  
Opeoluwa O. Oyedeji ◽  
Blessing A. Aderibigbe
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Mechanical Performance ◽  
Healing Process ◽  
Aloe Vera ◽  
Antimicrobial Properties ◽  
Biological Properties ◽  
Wound Management ◽  
Wound Healing Process ◽  
Treatment Of Wounds

The treatment of wounds is one challenging biomedical field due to delayed wound healing common in chronic wounds. Several factors delay wound healing, including microbial infections, malnutrition, underlying physiological conditions, etc. Most of the currently used wound dressing materials suffer from poor antimicrobial properties, poor biodegradability and biocompatibility, and weak mechanical performance. Plant extracts, such as Aloe vera, have attracted significant attention in wound management because of their interesting biological properties. Aloe vera is composed of essential constituents beneficial for the wound healing process, such as amino acids, vitamins C and E, and zinc. Aloe vera influences numerous factors that are involved in wound healing and stimulates accelerated healing. This review reports the therapeutic outcomes of aloe vera extract-loaded polymer-based scaffolds in wound management.

Identifying New Pathways and Targets for Wound Healing and Therapeutics from Natural Sources

2021 ◽  
Vol 18 ◽  
Author(s):  
Xinchi Feng ◽  
Jinsong Hao
Keyword(s):  
Wound Healing ◽  
Molecular Mechanisms ◽  
Chronic Wounds ◽  
Wound Care ◽  
Healing Process ◽  
Unmet Need ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Natural Sources ◽  
Viable Approach

: Chronic wounds remain a significant public problem and the development of wound treatments has been a research focus for the past few decades. Despite advances in the products derived from endogenous substances involved in a wound healing process (e.g. growth factors, stem cells, and extracellular matrix), effective and safe wound therapeutics are still limited. There is an unmet need to develop new therapeutics. Various new pathways and targets have been identified and could become a molecular target in designing novel wound agents. Importantly, many existing drugs that target these newly identified pathways could be repositioned for wound therapy, which will facilitate fast translation of research findings to clinical applications. This review discusses the newly identified pathways/targets and their potential uses in the development of wound therapeutics. Some herbs and amphibian skins have been traditionally used for wound repairs and their active ingredients have been found to act in these new pathways. Hence, screening these natural products for novel wound therapeutics remains a viable approach. The outcomes of wound care using natural wound therapeutics could be improved if we can better understand their cellular and molecular mechanisms and fabricate them in appropriate formulations, such as using novel wound dressings and nano-engineered materials. Therefore, we also provide an update on the advances in the wound therapeutics from natural sources. Overall, this review offers new insights into novel wound therapeutics.

scholarly journals Bioactive Agent-Loaded Electrospun Nanofiber Membranes for Accelerating Healing Process: A Review

Membranes ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 702
Author(s):  
Seyyed-Mojtaba Mousavi ◽  
Zohre Mousavi Nejad ◽  
Seyyed Alireza Hashemi ◽  
Marjan Salari ◽  
Ahmad Gholami ◽  
...  
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Therapeutic Potential ◽  
Healing Process ◽  
Wound Dressings ◽  
Future Research ◽  
Wound Healing Process ◽  
Nanofiber Membranes ◽  
Bioactive Agents ◽  
Bioactive Agent

Despite the advances that have been achieved in developing wound dressings to date, wound healing still remains a challenge in the healthcare system. None of the wound dressings currently used clinically can mimic all the properties of normal and healthy skin. Electrospinning has gained remarkable attention in wound healing applications because of its excellent ability to form nanostructures similar to natural extracellular matrix (ECM). Electrospun dressing accelerates the wound healing process by transferring drugs or active agents to the wound site sooner. This review provides a concise overview of the recent developments in bioactive electrospun dressings, which are effective in treating acute and chronic wounds and can successfully heal the wound. We also discuss bioactive agents used to incorporate electrospun wound dressings to improve their therapeutic potential in wound healing. In addition, here we present commercial dressings loaded with bioactive agents with a comparison between their features and capabilities. Furthermore, we discuss challenges and promises and offer suggestions for future research on bioactive agent-loaded nanofiber membranes to guide future researchers in designing more effective dressing for wound healing and skin regeneration.

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

scholarly journals Photocrosslinking silver nanoparticles–aloe vera–silk fibroin composite hydrogel for treatment of full-thickness cutaneous wounds

2021 ◽  
Vol 8 (6) ◽  
Author(s):  
Yangkun Liu ◽  
JinChuan Fan ◽  
MingQi Lv ◽  
Kepeng She ◽  
Jiale Sun ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Silver Nanoparticles ◽  
Silk Fibroin ◽  
Aloe Vera ◽  
Antibacterial Properties ◽  
Composite Hydrogel ◽  
Wound Dressings ◽  
Full Thickness ◽  
Cutaneous Wounds

Abstract Damage to the skin causes physiological and functional issues. The most effective treatment approach is the use of wound dressings. Silk fibroin (SF) is a promising candidate biomaterial for regulating wound healing; however, its antibacterial properties and biological activity must be further improved. In this study, a photocrosslinking hydrogel was developed to treat full-thickness cutaneous wounds. The composite hydrogel (Ag–AV–SF hydrogel) was prepared by introducing the silver nanoparticles (AgNPs) and aloe vera (AV) as the modifiers. In vitro study exhibited great antibacterial ability, biocompatibility and cell-proliferation and -migration-promoting capacities. It also showed the pH-response releasing properties which release more AgNPs in a simulated chronic infection environment. The healing effect evaluation in vivo showed the healing-promoting ability of the Ag–AV–SF hydrogel was stronger than the single-modifiers groups, and the healing rate of it reached 97.02% on Day 21, higher than the commercial wound dressing, silver sulfadiazine (SS) cream on sale. Additionally, the histological and protein expression results showed that the Ag–AV–SF hydrogel has a greater effect on the pro-healing regenerative phenotype with M2 macrophages at the early stage, reconstructing the blood vessels networks and inhibiting the formation of scars. In summary, the Ag–AV–SF hydrogel developed in this study had good physical properties, overwhelming antibacterial properties, satisfactory biocompatibility and significantly promoting effect on cell proliferation, migration and wound healing. Overall, our results suggest that the Ag–AV–SF hydrogel we developed has great potential for improving the wound healing in clinical treatment.

scholarly journals The Effect of Aloe vera (Linn) On Cutaneous Wound Healing and Wound Contraction Rate in Adult Rabbits

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Martha Orendu Attah ◽  
Tonye Watson Jacks ◽  
Attah Jacob ◽  
Otong Eduitem ◽  
Barnabas John
Keyword(s):  
Wound Healing ◽  
Aloe Vera ◽  
Wound Contraction ◽  
Control Group ◽  
Cutaneous Wound Healing ◽  
Contraction Rate ◽  
Wound Area ◽  
Cutaneous Wound ◽  
Cutaneous Wounds ◽  
Aloe Vera Gel

Background: In the present research study, the rate of cutaneous wound healing and contraction rate in healthy rabbits using Aloe vera pulp was studied.Methods: Ten healthy rabbits were used for the study. They were divided into two groups consisting of five rabbits each. Cutaneous wounds were made on the lumbar region of each rabbit using a template which ensured that the wounds were of the same size in all the rabbits. 5ml of Aloe vera gel was applied to the wounds of the animals in the test group, while nothing was applied to the wound area of the animals in the control group. The wound area in each group was measured for a period of 21 days, using a venire caliper and tracing paper which was used to trace the wound area. Tissue samples were removed from the wound area in both experimental and control groups and subjected to routine histological analysis, also, morphometric analysis was performed.Results: The rate of wound contraction and mean centripetal contraction was calculated in both groups and graphically represented using Microsoft Excel. The results showed that animals who were treated with Aloe vera gel had a greater wound contraction rate, as well as rapid wound closure. The micrographs showed a thicker epithelial layer, with thinner collagen fibers in the dermis of experimental animals compared to the control group. There was also an abundant capillary bed at the dermal-epidermal junction in the experimental group, compared to the control group.Conclusion:  Aloe vera may increase the rate of wound healing by accelerating epithelial migration, and may also play a role in neo-vascularization of the newly healed area.Keywords: Aloe vera, Cutaneous Wounds, Rabbits, Wound Contraction, Wound Healing

scholarly journals Perbandingan Pemberian Madu Lokal Bunga Cengkeh (Syzygium aromaticum) dan Madu Impor Bunga Manuka (Leptospermum scoparium) Secara Topikal Terhadap Penyembuhan Luka Sayat Pada Mencit Putih (Mus musculus)

2019 ◽  
Vol 6 (2) ◽  
pp. 25
Author(s):  
Nikita Tenritojang Mustafa ◽  
Dini Kurnia Ikliptikawati ◽  
Abdul Wahid Jamaluddin
Keyword(s):  
Wound Healing ◽  
Povidone Iodine ◽  
Healing Process ◽  
Colour Change ◽  
Negative Control Group ◽  
Negative Control ◽  
Wound Dressings ◽  
Control Group ◽  
Wound Healing Process ◽  
Syzygium Aromaticum

ABSTRAK Luka merupakan trauma fisik mengakibatkan kulit terbuka. Luka merupakan jalur masuk bagi mikroorganisme dari luar penyebab infeksi. Dahulu madu dimanfaatkan untuk mengobati luka bakar dan luka sayat. Madu memiliki keunggulan yakni memiliki hidrogen peroksida, eugenol dan methylglyoxal yang merupakan antibiotik yang unggul. Penelitian ini dilakukan dengan uji ekperimental. Hewan coba dibagi dalam 4 kelompok. Setiap kelompok diberikan perlakukan antara lain: dengan pemberian madu lokal, madu impor, dan salep povidone iodine (kontrol positif), dan kelompok kontrol negatif tanpa perlakuan. Dilanjutkan pemantauan dan pencatatan untuk proses penyembuhan tiap kelompok. Hal ini diukur dengan indikator antara lain: luas luka, kelembaban luka, warna, serta perubahan keropeng. Pada penelitian ini ditemukan bahwa dengan pemberian madu mempercepat proses penyembuhan luka dibandingkan dengan pemberian air dan povidone iodine (p<0,05). Selain itu ditemukan madu lokal memberikan efek penyembuhan luka yang lebih cepat dibandingkan madu impor. Pemberian madu memberikan efek yang lebih baik terhadap penyembuhan luka. Dibandingkan dengan pemberian salep Povidone iodine dan tanpa perlakuan.Kata kunci : Madu Bunga Cengkeh, Madu Bunga Manuka, Waktu penyebuhan, Warna Luka, Kelembaban. ABSTRACT Wound is a physical trauma causes discontinuity, Leads physiological dysfunction. Wound is an entry point for microorganisms from outside as source of infection. In the past, honey was used to treat burns and cuts. Honey is a natural ingredient as a moist wound-dressings. Besides that, honey contain hydrogen peroxide, eugenol, and methylglyoxal which is a superior antibiotic. This research was carried out by experimental test. The experimental animals were divided into 4 groups, each group consisted of 6 animals. Each group is treated, among others; by giving local honey, imported honey, povidone iodine (positive control), and negative control group without a treatment. Continued monitoring and recording for the healing process of each group. Statistical analysis is performed by calculation of frequency distribution and Independent-t, Anova, and regression analysis to determine the relationship between each variable. Statistical test results are significant if the value of p <0.05. This research found that addition of honeyaccelerated the wound healing process compared to the administration of water and povidone iodine (p <0.05). This is measured by indicators including; wide wounds, moisture wounds, colour change, and scab changes. By giving honey the perfect wound healing process begins on the 11th day until the 13th day. Besides that, local honey is found to provide healing effects that are faster than imported honey. Giving honey provides a better effect on wound healing. compared with Povidone iodine and without a treatment Keywords: Clove Flower Honey, Manuka Flower Honey, Time of Wasting, Colour of Wound, Moisture

scholarly journals Current Trends in Advanced Alginate-Based Wound Dressings for Chronic Wounds

2021 ◽  
Vol 11 (9) ◽  
pp. 890
Author(s):  
Andreea Barbu ◽  
Bogdan Neamtu ◽  
Marius Zăhan ◽  
Gabriela Mariana Iancu ◽  
Ciprian Bacila ◽  
...  
Keyword(s):  
Wound Healing ◽  
Chronic Inflammation ◽  
Wound Dressing ◽  
Chronic Wounds ◽  
Healing Process ◽  
Wound Dressings ◽  
In Vivo Studies ◽  
Public Health Issue ◽  
Wound Healing Process ◽  
Special Importance

Chronic wounds represent a major public health issue, with an extremely high cost worldwide. In healthy individuals, the wound healing process takes place in different stages: inflammation, cell proliferation (fibroblasts and keratinocytes of the dermis), and finally remodeling of the extracellular matrix (equilibrium between metalloproteinases and their inhibitors). In chronic wounds, the chronic inflammation favors exudate persistence and bacterial film has a special importance in the dynamics of chronic inflammation in wounds that do not heal. Recent advances in biopolymer-based materials for wound healing highlight the performance of specific alginate forms. An ideal wound dressing should be adherent to the wound surface and not to the wound bed, it should also be non-antigenic, biocompatible, semi-permeable, biodegradable, elastic but resistant, and cost-effective. It has to give protection against bacterial, infectious, mechanical, and thermal agents, to modulate the level of wound moisture, and to entrap and deliver drugs or other molecules This paper explores the roles of alginates in advanced wound-dressing forms with a particular emphasis on hydrogels, nanofibers networks, 3D-scaffolds or sponges entrapping fibroblasts, keratinocytes, or drugs to be released on the wound-bed. The latest research reports are presented and supported with in vitro and in vivo studies from the current literature.

scholarly journals Topical treatment of ointment containing ethanol extract of Archidendron pauciflorum fruit peel on the wound healing in streptozotocin-induced diabetic mice

2017 ◽  
Vol 9 (3) ◽  
pp. 306-311 ◽  
Author(s):  
DESAK MADE MALINI ◽  
MADIHAH MADIHAH ◽  
FITRI KAMILAWATI ◽  
NINING RATNINGSIH ◽  
KARTIAWATI ALIPIN ◽  
...  
Keyword(s):  
Wound Healing ◽  
Topical Application ◽  
Topical Treatment ◽  
Healing Process ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Diabetic Mice ◽  
Fruit Peel ◽  
Diabetic Wounds

Malini DM, Madihah, Kamilawati F, Ratningsih N, Alipin K, Iskandar J. 2017. Topical treatment of ointment containing ethanol extract of Archidendron pauciflorum fruit peel on the wound healing in streptozotocin-induced diabetic mice. Nusantara Bioscience 9: 306-311. Diabetic wounds lead to severe tissue damage and are difficult to cure. One alternative medicine known well by local Indonesian communities to treat diabetic wounds is the fruit peel of djengkol. This study aimed to evaluate the ointment containing ethanol extract of djengkol fruit peel to accelerate wound healing process in the skin of streptozotocin-induced diabetic mice. The method was experimental using completely randomized design with six treatments and four replications. Diabetes was induced by intraperitoneal injection of streptozotocin 180 mg/kg BW. Mice with blood glucose level ≥150 mg/dL were used for diabetic mice models. The incision wound created at dorsolateral region of shaven skin at ±1 cm2 using sterile scissors. The ointment containing extract was applied topically to the diabetic mice wounds at concentration of 5%, 10% and 15%, as well as Betadine® as the reference group. The ointment basis was applied to the wound of diabetic mice as a positive control and to the wound of non-diabetic mice as a negative control. The treatment was done twice a day for 14 days. The results showed that topical application of ointment containing ethanol extract of djengkol fruit peel at concentration of 10% gave significant recovery (p<0.05) on the wounded skin by the enhancement of re-epithelization and granulation tissue, as well as the increase of capillary number and collagen density which were higher than other treatments and comparable to negative control group. It was concluded that the topical application of ointment containing ethanol extract of djengkol fruit peel can support the healing of diabetic wounds.

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