The apple columnar gene candidate MdCoL and the AP2/ERF factor MdDREB2 positively regulate ABA biosynthesis by activating the expression of MdNCED6/9

2020 ◽  
Author(s):  
Xin Sun ◽  
Cuiping Wen ◽  
Jihua Xu ◽  
Yihe Wang ◽  
Jun Zhu ◽  
...  
Keyword(s):  
Gene Promoters ◽  
Apple Trees ◽  
Columnar Growth ◽  
Interaction Experiment ◽  
Growth Phenotype ◽  
Strong Candidate ◽  
Transcription Factor Expression ◽  
Aba Content ◽  
Aba Biosynthesis ◽  
Strong Candidate Gene

Abstract MdCoL, which encodes a putative 2OG-Fe(II) oxygenase, is a strong candidate gene for control of the columnar growth phenotype in apple. However, the mechanism by which MdCoL could produce the columnar trait is unclear. Here, we show that MdCoL influences ABA biosynthesis through its interactions with the MdDREB2 transcription factor. Expression analyses and transgenic tobacco studies confirmed that MdCoL is likely a candidate for control of the columnar phenotype. Furthermore, the ABA level in columnar apple trees is significantly higher than that in standard apple trees. A protein interaction experiment showed that MdCoL interacts with MdDREB2. Transient expression and EMSA assays demonstrated that MdDREB2 binds directly to the DRE motif in the MdNCED6 and MdNCED9 (MdNCED6/9) gene promoters, thereby activating the transcription of these ABA biosynthesis genes. In addition, a higher ABA content was detected following co-overexpression of MdCoL–MdDREB2 when compared with the overexpression of MdCoL or MdDREB2 alone. Taken together, our results indicate that an interaction between MdCoL and MdDREB2 promotes the expression of MdNCED6/9 and increases ABA levels, a phenomenon that may underlie the columnar growth phenotype in apple.

Vegetation-derived abscisic acid and four terpenes enforce dormancy in seeds of the post-fire annual,Nicotiana attenuata

2002 ◽  
Vol 12 (4) ◽  
pp. 239-252 ◽  
Author(s):  
Bernd Krock ◽  
Sybille Schmidt ◽  
Christian Hertweck ◽  
Ian T. Baldwin
Keyword(s):  
Abscisic Acid ◽  
Environmental Control ◽  
Wood Smoke ◽  
Nicotiana Attenuata ◽  
Germination Inhibitors ◽  
Physiological Basis ◽  
Fire Environment ◽  
Aba Content ◽  
Aba Biosynthesis ◽  
Endogenous Aba

AbstractThe native tobacco,Nicotiana attenuata, synchronizes its germination with the immediate post-fire environment with a combination of germination stimulants found in wood smoke and inhibitors from the unburned litter of the dominant vegetation. The inhibitors override the stimulants and prevent seeds from germinating maladaptively in unburned habitats adjacent to burns. To understand the physiological basis of this environmental control of germination, we tested several previously isolated signals, phytohormones and their respective biosynthesis inhibitors. The germination inhibitors methyl jasmonate (MeJA, a constituent of sagebrush litter), bornane-2,5-dione (BD, a constituent of juniper litter extract, JLE) and JLE did not alter abscisic acid (ABA) content of imbibed seeds. Treatment with the ABA biosynthesis inhibitor, fluridone, inhibited the dormancy-inducing effects of BD, JLE and MeJA, but surprisingly did not affect endogenous ABA levels in treated seeds. However, ABA leached from litter of the species, which dominate the plant community before fires, plays an important role in germination control. We conclude thatN. attenuataseeds, which can lie dormant in the soil for 150 years between fires, time their germination with the post-fire environment by responding to smoke, ABA and four terpenes (BD, 1,8-cineole, β-thujaplicin and camphor) leaching from the litter of the dominant vegetation.

P95 MRAS – A STRONG CANDIDATE GENE (SCG) FOR CORONARY ARTERY DISEASE (CAD)

2010 ◽  
Vol 11 (2) ◽  
pp. 36
Author(s):  
J. Freyer ◽  
A.K. Wagner ◽  
H. Schunkert ◽  
J.-J. Zwaginga ◽  
Z. Aherrahrou ◽  
...  
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Candidate Gene ◽  
Artery Disease ◽  
Strong Candidate ◽  
Strong Candidate Gene

scholarly journals <i>UBXN1</i> is a strong candidate gene in regulation of pork water-holding capacity

2021 ◽  
Vol 64 (1) ◽  
pp. 109-118
Author(s):  
Jiawen He ◽  
Xiangsheng Lin ◽  
Haoxin Yang ◽  
Ye Tian ◽  
Yuelei Zhao ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Promoter Region ◽  
Mrna Level ◽  
Water Holding Capacity ◽  
Luciferase Assay ◽  
Large White ◽  
Allele Distribution ◽  
Strong Candidate ◽  
Water Holding ◽  
Strong Candidate Gene

Abstract. The UBX domain containing protein 1-like gene (UBXN1) promotes the degradation of myofibrillar proteins during meat maturation, which affects meat water-holding capacity (WHC). This study aims to identify functional mutations in UBXN1 promoter region, which affects the transcription activity and therefore the WHC. Firstly, we confirmed that the UBXN1 expression level was positively associated with WHC. Individuals with high and low WHC (n=16 per group) were selected from 168 Duroc × Large White × Yorkshire (D × L × Y) crossbred pigs. The UBXN1 promoter region was comparatively sequenced using DNA pools from these two groups, and a mutation ca. −379T > G was revealed that had reverse allele distribution. The single nucleotide polymorphism (SNP) was then genotyped in the abovementioned population. TT genotype individuals exhibited higher UBXN1 mRNA level and higher WHC compared with GG genotype ones. Further luciferase assay confirmed that TT genotype promoter had higher activity. Moreover, the degradation of cytoskeletal framework proteins of muscle cells like desmin, synemin, dystrophin, and vinculin was higher in TT genotype individuals than GG ones. In conclusion, we identified a SNP in the UBXN1 gene promoter that contributes to WHC improvement and pork quality. And UBXN1 is a strong candidate gene in regulation of pork WHC.

scholarly journals Columnar growth phenotype in apple results from gibberellin deficiency by ectopic expression of a dioxygenase gene

2020 ◽  
Vol 40 (9) ◽  
pp. 1205-1216
Author(s):  
Kazuma Okada ◽  
Masato Wada ◽  
Yumiko Takebayashi ◽  
Mikiko Kojima ◽  
Hitoshi Sakakibara ◽  
...  
Keyword(s):  
Ectopic Expression ◽  
Lateral Branch ◽  
Flower Bud ◽  
Columnar Growth ◽  
Tobacco Plants ◽  
Bud Formation ◽  
Aerial Parts ◽  
Growth Phenotype ◽  
Flower Bud Formation ◽  
Lateral Branches

Abstract The apple cultivar McIntosh Wijcik, which is a mutant of ‘McIntosh’, exhibits a columnar growth phenotype (short internodes, few lateral branches, many spurs, etc.) that is controlled by a dominant Co gene. The candidate gene (MdDOX-Co), encoding a 2-oxoglutarate-dependent dioxygenase, is located adjacent to an insertion mutation. Non-columnar apples express MdDOX-Co in the roots, whereas columnar apples express MdDOX-Co in the aerial parts as well as in the roots. However, the function of MdDOX-Co remains unknown. Here, we characterized tobacco plants overexpressing MdDOX-Co. The tobacco plants showed the typical dwarf phenotype, which was restored by application of gibberellin A3 (GA3). Moreover, the dwarf tobacco plants had low concentrations of endogenous bioactive gibberellin A1 (GA1) and gibberellin A4 (GA4). Similarly, ‘McIntosh Wijcik’ contained low endogenous GA4 concentration and its dwarf traits (short main shoot and internodes) were partially reversed by GA3 application. These results indicate that MdDOX-Co is associated with bioactive GA deficiency. Interestingly, GA3 application to apple trees also resulted in an increased number of lateral branches and a decrease in flower bud number, indicating that gibberellin (GA) plays important roles in regulating apple tree architecture by affecting both lateral branch formation (vegetative growth) and flower bud formation (reproductive growth). We propose that a deficiency of bioactive GA by ectopic expression of MdDOX-Co in the aerial parts of columnar apples not only induces dwarf phenotypes but also inhibits lateral branch development and promotes flower bud formation, and assembly of these multiple phenotypes constructs the columnar tree form.

scholarly journals A strong candidate gene for the Papg1 locus on mouse chromosome 4 affecting lung tumor progression

Oncogene ◽  
2002 ◽  
Vol 21 (38) ◽  
pp. 5960-5966 ◽  
Author(s):  
Zhongqiu Zhang ◽  
Yian Wang ◽  
Christopher R Herzog ◽  
Gongjie Liu ◽  
Han-Woong Lee ◽  
...  
Keyword(s):  
Tumor Progression ◽  
Candidate Gene ◽  
Mouse Chromosome ◽  
Lung Tumor ◽  
Chromosome 4 ◽  
Strong Candidate ◽  
Strong Candidate Gene

Identification of an Alu retrotransposition event in close proximity to a strong candidate gene for Huntington's disease

Nature ◽  
1993 ◽  
Vol 362 (6418) ◽  
pp. 370-373 ◽  
Author(s):  
Y. Paul Goldberg ◽  
Johanna M. Rommens ◽  
Susan E. Andrew ◽  
Gordon B. Hutchinson ◽  
Biaoyang Lin ◽  
...  
Keyword(s):  
Huntington's Disease ◽  
Candidate Gene ◽  
Huntington’S Disease ◽  
Close Proximity ◽  
Strong Candidate ◽  
Strong Candidate Gene

scholarly journals Two loci on chromosome 9 control bile acid composition: evidence that a strong candidate gene,Cyp8b1, is not the culprit

2006 ◽  
Vol 47 (9) ◽  
pp. 2020-2027 ◽  
Author(s):  
Ephraim Sehayek ◽  
Lee R. Hagey ◽  
Yee-Yan Fung ◽  
Elizabeth M. Duncan ◽  
Hannah J. Yu ◽  
...  
Keyword(s):  
Bile Acid ◽  
Candidate Gene ◽  
Acid Composition ◽  
Chromosome 9 ◽  
Strong Candidate ◽  
Strong Candidate Gene
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