scholarly journals Physiological responses of Norway spruce (Picea abies) seedlings to drought stress

2009 ◽  
Vol 30 (2) ◽  
pp. 205-213 ◽  
Author(s):  
L. Ditmarova ◽  
D. Kurjak ◽  
S. Palmroth ◽  
J. Kmet ◽  
K. Strelcova
Keyword(s):  
Drought Stress ◽  
Picea Abies ◽  
Norway Spruce ◽  
Physiological Responses

scholarly journals Drought Stress Described by Transcriptional Responses of Picea Abies under Pathogen Heterobasidion Parviporum Attack

2021 ◽  
Author(s):  
Xenia Hao-Yi Yeoh ◽  
Blessing Durodola ◽  
Kathrin Blumenstein ◽  
Eeva Terhonen
Keyword(s):  
Climate Change ◽  
Drought Stress ◽  
Picea Abies ◽  
Norway Spruce ◽  
Water Availability ◽  
Differentially Expressed Gene ◽  
Differentially Expressed ◽  
Gene Expressions ◽  
Transcriptional Responses ◽  
Water Group

The major threats to the sustainable supply of forest tree products are adverse climate, pests and diseases. Climate change, exemplified by increased drought, poses a unique threat to global forest health. This is attributed to the unpredictable behavior of forest pathosystems, which can favor fungal pathogens over the host under persistent drought stress conditions in the future. Currently, the effects of drought on tree resistance against pathogens are hypothetical, thus research is needed to identify these correlations. Norway spruce (Picea abies) is one of the most economically important tree species in Europe, and is considered highly vulnerable to changes in climate. Dedicated experiments to investigate how disturbances will affect the Norway spruce - Heterobasidion sp. pathosystem are important, in order to develop different strategies to limit the spread of H. annosum s.l. under the predicted climate change. Here, we report a transcriptional study to compare Norway spruce gene expressions to evaluate the effects of water availability and the infection of Heterobasidion parviporum. We performed inoculation studies of three-year-old saplings in a greenhouse (purchased from a nursery). Norway spruce saplings were treated in either high (+) or low (-) water groups: high water group received double the water amount than the low water group. RNA was extracted and sequenced. Similarly, we quantified gene expression levels of candidate genes in biotic stress and jasmonic acid (JA) signaling pathways using qRT-PCR, through which we discovered a unique preferential defense response of H. parviporum-infected Norway spruce under drought stress at the molecular level. Disturbances related to water availability, especially low water conditions can have negative effects on the tree host and benefit the infection ability of the pathogens in the host. From our RNA-seq analysis, 114 differentially expressed gene regions were identified between high (+) and low (-) water groups under pathogen attack. None of these gene pathways were identified to be differentially expressed from both non-treated and mock-control treatments between high (+) and low (-) water groups. Finally, only four genes were found to be associated with drought in all treatments.

scholarly journals Effects of ozone and drought stress on the physiology and growth of two clones of Norway spruce (Picea abies)

1997 ◽  
Vol 136 (2) ◽  
pp. 265-275 ◽  
Author(s):  
P. E. KARLSSON ◽  
E. L. MEDIN ◽  
G. WALLIN ◽  
G. SELLDEN ◽  
L. SKARBY
Keyword(s):  
Drought Stress ◽  
Picea Abies ◽  
Norway Spruce

Alteration of element contents in guard cells of Norway spruce (Picea abies) subjected to ozone fumigation and (or) water stress: X-ray microanalysis study

1994 ◽  
Vol 72 (1) ◽  
pp. 86-92 ◽  
Author(s):  
D. Le Thiec ◽  
C. Rose ◽  
J. P. Garrec ◽  
D. Laffray ◽  
P. Louguet ◽  
...  
Keyword(s):  
Drought Stress ◽  
Picea Abies ◽  
Norway Spruce ◽  
Manganese Content ◽  
Stomatal Closure ◽  
Calcium Content ◽  
Guard Cells ◽  
Potassium Content ◽  
X Ray ◽  
Simultaneous Implementation

Two clones of Norway spruce Picea abies (L.) Karst. (Gerardmer clone and Istebna clone) were subjected to an ozone-enriched atmosphere. The X-ray microanalysis of elements present in the guard cells showed a decrease in potassium for the Gerardmer clone along with a larger closing of guard cells and an increase in calcium contents; for the Istebna clone, ozone did not affect these contents. When a drought stress was applied, an increase in calcium content of guard cells of the Istebna clone was also found. However, the potassium content was smaller in this case for the two clones and could well be due to stomatal closure. When ozone and drought stress were applied together, the potassium content in the Istebna clone was not affected, but for the Gerardmer clone, ozone reduced the decrease in potassium content that occurred as drought stress developed. However, calcium (and manganese) content increased significantly when the Gerardmer clone was exposed to ozone. It seems likely that the Gerardmer clone is sensitive to ozone and when subjected to a drought stress this sensitivity is increased. For the Istebna clone, the resistance to ozone is unaffected by the drought stress treatment. The Istebna clone, however, seems sensitive to drought. These characteristic responses in the two different Norway spruce clones are probably due to genetic differences and may explain the contradictory observations in the response of the stomata during the simultaneous implementation of the complex stresses. Key words: Picea abies, guard cells, ozone, drought stress, X-ray microanalysis, elements.

scholarly journals Growth and Physiological Responses of Norway Spruce (Picea abies (L.) H. Karst) Supplemented with Monochromatic Red, Blue and Far-Red Light

Forests ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 164
Author(s):  
Fangqun OuYang ◽  
Yang Ou ◽  
Tianqin Zhu ◽  
Jianwei Ma ◽  
Sanping An ◽  
...  
Keyword(s):  
Gene Expression ◽  
Electron Transport ◽  
Picea Abies ◽  
Norway Spruce ◽  
Light Quality ◽  
Physiological Responses ◽  
Red Light ◽  
Variable Fluorescence ◽  
Related Gene ◽  
Bisphosphate Carboxylase

Monochromatic red light (R) supplementation is more efficient than blue light (B) in promoting Norway spruce (Picea abies (L.) H. Karst) growth. Transcriptome analysis has revealed that R and B may regulate stem growth by regulating phytohormones and secondary metabolites; however, the effects of light qualities on physiological responses and related gene expression in Norway spruce require further study. In the present study, three-year-old Norway spruce seedlings received sunlight during the daytime were exposed to monochromatic B (460 mm), monochromatic R (660 nm), monochromatic far-red light (FR, 730 nm), and a combination of three monochromatic lights (control, R:FR:B = 7:1:1) using light-emitting diode (LED) lamps for 12 h after sunset for 90 day. Growth traits, physiological responses, and related gene expression were determined. The results showed that light quality significantly affected Norway spruce growth. The stem height, root collar diameter, and current-year shoot length of seedlings treated with R were 2%, 10% and 12% higher, respectively, than those of the control, whereas seedlings treated with B and FR showed significantly lower values of these parameters compared with that of the control. The net photosynthetic rate (Pn) of seedlings under R treatment was 10% higher than that of the control, whereas the Pn values of seedlings treated with FR and B were 22% and 33%, respectively, lower than that of the control. The ratio of phosphoenolpyruvate carboxylase to ribulose-1,5-bisphosphate carboxylase/oxygenase (PEPC/Rubisco) of seedlings after the R treatment (0.581) was the highest and 3.98 times higher than that of the seedlings treated with B. Light quality significantly affected the gibberellic acid (GAs) levels, which was 13% higher in seedlings treated with R (6.4 g/100 ng) than that of the control, whereas, the GAs level of seedlings treated with B and FR was 17% and 19% lower, respectively, than that of the control. In addition, seedlings treated with R achieved the lowest ratio of leaf chlorophyll content to fresh weight (8.7). Compared to the R and control treatments, seedlings received FR treatment had consistently lower values of the quantum yield of electron transport beyond QA− (primary quinone, ϕEo) and efficiency, with which a trapped exciton moves an electron into the electron transport chain beyond QA− (ψo), while higher values of the relatively variable fluorescence at the J step and normalized relatively variable fluorescence at the K step (Wk). The values of ϕEo, ψO, VJ and Wk in seedlings treated with B were similar to those in the control group. The expression of genes associated with light signal transduction, such as PHYTOCHROME C (PHYC), ELONGATED HYPOCOTYL5 (HY5), CONSTITUTIVE PHOTOMORPHOGENIC 1-2 (COP1-2), and PHYTOCHROMEINTERACTING FACTOR 3 (PIF3), was significantly higher in seedlings under B treatment than those under other light treatments. Nevertheless, significant differences were not observed in the expression of COP1-2, HY5, and PIF3 between the R treatment and the control. The expression value of COP1-2 was significantly lower in R than FR light treatments. In conclusion, compared with the control, R promotes, whereas B and FR inhibit Norway spruce growth, which was accompanied by physiological changes and genes expression regulation that may be relate to a changing phytochrome photostationary state (PSS) with the supplemental R in seedlings.

Assessing seasonal drought stress response in Norway spruce (Picea abies(L.) Karst.) by monitoring stem circumference and sap flow

Ecohydrology ◽  
2014 ◽  
Vol 8 (3) ◽  
pp. 378-386 ◽  
Author(s):  
Marek Ježík ◽  
Miroslav Blaženec ◽  
Matthew G. Letts ◽  
Ľubica Ditmarová ◽  
Zuzana Sitková ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Picea Abies ◽  
Norway Spruce ◽  
Sap Flow ◽  
Seasonal Drought

scholarly journals Drought Stress Described by Transcriptional Responses of Picea Abies (L.) H. Karst. under Pathogen Heterobasidion Parviporum Attack

Forests ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1379
Author(s):  
Xenia Hao-Yi Yeoh ◽  
Blessing Durodola ◽  
Kathrin Blumenstein ◽  
Eeva Terhonen
Keyword(s):  
Climate Change ◽  
Drought Stress ◽  
Picea Abies ◽  
Norway Spruce ◽  
Water Availability ◽  
Differentially Expressed Gene ◽  
Differentially Expressed ◽  
Gene Expressions ◽  
Transcriptional Responses ◽  
Water Group

The major threats to the sustainable supply of forest tree products are adverse climate, pests and diseases. Climate change, exemplified by increased drought, poses a unique threat to global forest health. This is attributed to the unpredictable behavior of forest pathosystems, which can favor fungal pathogens over the host under persistent drought stress conditions in the future. Currently, the effects of drought on tree resistance against pathogens are hypothetical, thus research is needed to identify these correlations. Norway spruce (Picea abies (L.) H. Karst.) is one of the most economically important tree species in Europe and is considered highly vulnerable to changes in climate. Dedicated experiments to investigate how disturbances will affect the Norway spruce—Heterobasidion sp. pathosystem are important, in order to develop different strategies to limit the spread of H. annosum s.l. under the predicted climate change. Here, we report a transcriptional study to compare Norway spruce gene expressions to evaluate the effects of water availability and the infection of Heterobasidion parviporum. We performed inoculation studies of three-year-old saplings in a greenhouse (purchased from a nursery). Norway spruce saplings were treated in either high (+) or low (−) water groups: high water group received double the water amount than the low water group. RNA was extracted and sequenced. Similarly, we quantified gene expression levels of candidate genes in biotic stress and jasmonic acid (JA) signaling pathways using qRT-PCR, through which we discovered a unique preferential defense response of H. parviporum-infected Norway spruce under drought stress at the molecular level. Disturbances related to water availability, especially low water conditions can have negative effects on the tree host and benefit the infection ability of the pathogens in the host. From our RNA-seq analysis, 114 differentially expressed gene regions were identified between high (+) and low (−) water groups under pathogen attack. None of these gene pathways were identified to be differentially expressed from both non-treated and mock-control treatments between high (+) and low (−) water groups. Finally, only four genes were found to be associated with drought in all treatments.

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