Identification of Lactic Acid Bacteria Using Temperature Gradient Gel Electrophoresis for DNA Fragments Amplified by Polymerase Chain Reaction

1994 ◽  
Vol 52 (3) ◽  
pp. 95-99 ◽  
Author(s):  
Youichi Tsuchiya ◽  
Yukinobu Kano ◽  
Shohei Koshino
Keyword(s):  
Polymerase Chain Reaction ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Temperature Gradient ◽  
Gel Electrophoresis ◽  
Dna Fragments ◽  
Chain Reaction ◽  
Gradient Gel Electrophoresis ◽  
Polymerase Chain ◽  
Temperature Gradient Gel Electrophoresis

Temperature Gradient Gel Electrophoresis of the Amplified Product of a Small 16S rRNA Gene Fragment for the Identification of Listeria Species Isolated from Food

2000 ◽  
Vol 63 (5) ◽  
pp. 659-661 ◽  
Author(s):  
MARISA MANZANO ◽  
LUCA COCOLIN ◽  
CARLO CANTONI ◽  
GIUSEPPE COMI
Keyword(s):  
Polymerase Chain Reaction ◽  
Temperature Gradient ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gel Electrophoresis ◽  
Rrna Gene ◽  
Chain Reaction ◽  
Gradient Gel Electrophoresis ◽  
Polymerase Chain ◽  
Temperature Gradient Gel Electrophoresis

The development of a rapid method for the identification of Listeria spp. is described. It is based on the polymerase chain reaction amplification of a small fragment from the 16S rRNA gene followed by temperature gradient gel electrophoresis. Forty-five strains of Listeria spp. (Listeria monocytogenes, Listeria innocua, Listeria ivanovii, Listeria seeligeri, and Listeria welshimeri) were used for the optimization of the protocol. No differences were observed between the results of the identification of the strains tested using traditional methods and those obtained by polymerase chain reaction–temperature gradient gel electrophoresis analysis.

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