Release of β-Glucan from Cell Walls of Starchy Endosperm of Barley

2001 ◽  
Vol 78 (2) ◽  
pp. 121-124 ◽  
Author(s):  
Makoto Kanauchi ◽  
Charles W. Bamforth
Keyword(s):  
Cell Walls ◽  
Starchy Endosperm

scholarly journals Cell Walls of Developing Wheat Starchy Endosperm: Comparison of Composition and RNA-Seq Transcriptome

2011 ◽  
Vol 158 (2) ◽  
pp. 612-627 ◽  
Author(s):  
Till K. Pellny ◽  
Alison Lovegrove ◽  
Jackie Freeman ◽  
Paola Tosi ◽  
Christopher G. Love ◽  
...  
Keyword(s):  
Cell Walls ◽  
Rna Seq ◽  
Starchy Endosperm

Isolation and Ultrastructure of Aleurone Cell Walls From Wheat and Barley

1981 ◽  
Vol 8 (5) ◽  
pp. 453 ◽  
Author(s):  
A Bacic ◽  
BA Stone
Keyword(s):  
Electron Microscopy ◽  
Cell Walls ◽  
Morphological Characteristics ◽  
Triticum Aestivum L ◽  
Whole Grains ◽  
Starchy Endosperm ◽  
Hordeum Vulgare L ◽  
Air Classification ◽  
Transmission Electron ◽  
Bilayered Structure

Aleurone layers and cell walls from both wheat (Triticum aestivum L. cv. Insignia) and barley (Hordeum vulgare L. cv. Clipper) have been isoIated by differential centrifugation in benzene-carbon tetrachloride mixtures and by air classification. The isolated walls were obtained in sufficient quantities and purity for comprehensive chemical analysis. Morphological characteristics of the isolated aleurone layers and walls were examined by bright field, fluorescence and scanning electron microscopy and compared with their appearance in whole grains. Transmission electron microscopy of wall sections clearly showed their characteristic bilayered structure. Aniline blue-positive deposits were observed at the aleurone-starchy endosperm interfaces of both wheat and barley.

scholarly journals Isolation and Purification of Cell Walls in the Starchy Endosperm of Rice Kernels, and the Aggregation of Sake Yeasts with the Cell Walls

1973 ◽  
Vol 47 (12) ◽  
pp. 763-769
Author(s):  
Nobuo SUGANO ◽  
Hiroichi AKIYAMA ◽  
Kikuo NOSHIRO
Keyword(s):  
Cell Walls ◽  
Starchy Endosperm ◽  
Isolation And Purification

Degradation of Starchy Endosperm Cell Walls in Nongerminating Sterilized Barley by Fungi

2001 ◽  
Vol 49 (2) ◽  
pp. 975-981 ◽  
Author(s):  
Iris Noots ◽  
Veerle Derycke ◽  
Kris Cornelis ◽  
Chris Michiels ◽  
Jan A. Delcour ◽  
...  
Keyword(s):  
Cell Walls ◽  
Endosperm Cell ◽  
Starchy Endosperm

Milling of Canadian oats and barley for functional food ingredients: Oat bran and barley fibre-rich fractions

2014 ◽  
Vol 94 (3) ◽  
pp. 573-586 ◽  
Author(s):  
Marta S. Izydorczyk ◽  
Tricia McMillan ◽  
Sharon Bazin ◽  
Jerry Kletke ◽  
Len Dushnicky ◽  
...  
Keyword(s):  
Functional Food ◽  
Cell Walls ◽  
Blood Cholesterol ◽  
Therapeutic Effects ◽  
Starchy Endosperm ◽  
Food Ingredients ◽  
Barley Cultivars ◽  
Oat Bran ◽  
Lower Porosity ◽  
Roller Milling

Izydorczyk, M. S., McMillan, T., Bazin, S., Kletke, J., Dushnicky, L., Dexter, J., Chepurna, A. and Rossnagel, B. 2014. Milling of Canadian oats and barley for functional food ingredients: Oat bran and barley fibre-rich fractions. Can. J. Plant Sci. 94: 573–586. Oats and barley are recognized for their valuable fibre constituents having protective and therapeutic effects against the development of diet-related disorders. Mixed linkage (1–3), (1–4)-β-D-glucans, the major dietary fibre constituents in oats and barley, have been linked to blood cholesterol lowering properties of these grains. The objective of this study was to compare oat bran and barley fibre-rich fractions (FRF) as two products with elevated levels of β-glucans and obtained by similar roller milling processes. The content of β-glucan in oat brans prepared from three different cultivars (AC Morgan, HiFi, and CDC ProFi) ranged from 7.90 to 9.50%, whereas the content of β-glucans in FRF prepared from three barley cultivars (CDC McGwire, CDC Fibar, and CDC Hilose) ranged from 9.31 to 18.19% (dwb). The yields of oat brans ranged from 44 to 49% and the yields of barley FRF from 39–49%. Both preparations contained higher amounts of arabinoxylans, proteins, and ash compared with the original grains. The oat brans were made up mainly of fragments containing the outer grain layers with a substantial portion of the subaleurone starchy endosperm attached to them, whereas the barley FRF consisted primarily of fragments containing the endosperm cell walls, with a smaller proportion of the outer grain tissues. The barley FRF contained smaller particles with broader distribution of particle size than the oat brans. The oat bran particles had higher bulk density and lower porosity than the barley FRF. Both preparations showed pronounced viscosity-building properties when dispersed in water at 45°C, but exhibited different viscosity profiles and differences in the attainable viscosity values.

Ferulic acid esterase catalyses the solubilization of ?-glucans and pentosans from the starchy endosperm cell walls of barley

1996 ◽  
Vol 18 (12) ◽  
pp. 1423-1426 ◽  
Author(s):  
J Moore ◽  
C W Bamforth ◽  
P A Kroon ◽  
B Bartolom� ◽  
G Williamson
Keyword(s):  
Ferulic Acid ◽  
Cell Walls ◽  
Endosperm Cell ◽  
Ferulic Acid Esterase ◽  
Starchy Endosperm

Ectodesmata as Revealed By Freeze-Etch Preparations of Plant Epidermal Cell Walls

1973 ◽  
Vol 31 ◽  
pp. 468-469
Author(s):  
N.C. Lyon ◽  
W. C. Mueller
Keyword(s):  
Electron Microscopy ◽  
Acetic Acid ◽  
Nitric Acid ◽  
Oxalic Acid ◽  
Light Microscopy ◽  
Epidermal Cell ◽  
Cell Walls ◽  
Plant Viruses ◽  
Plant Leaves ◽  
Thin Sections

Schumacher and Halbsguth first demonstrated ectodesmata as pores or channels in the epidermal cell walls in haustoria of Cuscuta odorata L. by light microscopy in tissues fixed in a sublimate fixative (30% ethyl alcohol, 30 ml:glacial acetic acid, 10 ml: 65% nitric acid, 1 ml: 40% formaldehyde, 5 ml: oxalic acid, 2 g: mecuric chloride to saturation 2-3 g). Other workers have published electron micrographs of structures transversing the outer epidermal cell in thin sections of plant leaves that have been interpreted as ectodesmata. Such structures are evident following treatment with Hg++ or Ag+ salts and are only rarely observed by electron microscopy. If ectodesmata exist without such treatment, and are not artefacts, they would afford natural pathways of entry for applied foliar solutions and plant viruses.

An ultrastructural study of vegetative incompatibility in plants

1978 ◽  
Vol 36 (2) ◽  
pp. 436-437
Author(s):  
Randy Moore
Keyword(s):  
Ultrastructural Study ◽  
Cell Walls ◽  
Growth And Development ◽  
Solanum Pennellii ◽  
Initial Product ◽  
Basic Aspect ◽  
Tissue Interactions ◽  
Graft Interface ◽  
Antigen Antibody ◽  
Standard Fixation

Cell and tissue interactions are a basic aspect of eukaryotic growth and development. While cell-to-cell interactions involving recognition and incompatibility have been studied extensively in animals, there is no known antigen-antibody reaction in plants and the recognition mechanisms operating in plant grafts have been virtually neglected.An ultrastructural study of the Sedum telephoides/Solanum pennellii graft was undertaken to define possible mechanisms of plant graft incompatibility. Grafts were surgically dissected from greenhouse grown plants at various times over 1-4 weeks and prepared for EM employing variations in the standard fixation and embedding procedure. Stock and scion adhere within 6 days after grafting. Following progressive cell senescence in both Sedum and Solanum, the graft interface appears as a band of 8-11 crushed cells after 2 weeks (Fig. 1, I). Trapped between the buckled cell walls are densely staining cytoplasmic remnants and residual starch grains, an initial product of wound reactions in plants.

Comparison of Substructures Between Uniaxial and Biaxial Deformation in 1100-0 Aluminum

1981 ◽  
Vol 39 ◽  
pp. 52-53
Author(s):  
D. L. Rohr ◽  
S. S. Hecker
Keyword(s):  
Plastic Strain ◽  
Cell Walls ◽  
Room Temperature ◽  
Mechanical Response ◽  
Biaxial Tension ◽  
Initial Deformation ◽  
Uniaxial Deformation ◽  
Biaxial Deformation ◽  
Stress States ◽  
Comprehensive Study

As part of a comprehensive study of microstructural and mechanical response of metals to uniaxial and biaxial deformations, the development of substructure in 1100 A1 has been studied over a range of plastic strain for two stress states.Specimens of 1100 aluminum annealed at 350 C were tested in uniaxial (UT) and balanced biaxial tension (BBT) at room temperature to different strain levels. The biaxial specimens were produced by the in-plane punch stretching technique. Areas of known strain levels were prepared for TEM by lapping followed by jet electropolishing. All specimens were examined in a JEOL 200B run at 150 and 200 kV within 24 to 36 hours after testing.The development of the substructure with deformation is shown in Fig. 1 for both stress states. Initial deformation produces dislocation tangles, which form cell walls by 10% uniaxial deformation, and start to recover to form subgrains by 25%. The results of several hundred measurements of cell/subgrain sizes by a linear intercept technique are presented in Table I.

Sign in / Sign up

Export Citation Format

Share Document