scholarly journals Lettuce mosaic virus Pathogenicity Determinants in Susceptible and Tolerant Lettuce Cultivars Map to Different Regions of the Viral Genome

2001 ◽  
Vol 14 (6) ◽  
pp. 804-810 ◽  
Author(s):  
Elise Redondo ◽  
Renate Krause-Sakate ◽  
Shu-Jun Yang ◽  
Hervé Lot ◽  
Olivier Le Gall ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Genes ◽  
Cdna Clones ◽  
Mild Mosaic ◽  
Recessive Resistance ◽  
Lettuce Mosaic Virus ◽  
Severe Stunting ◽  
Recessive Genes ◽  
Leaf Deformation ◽  
Virus Isolates

Full-length infectious cDNA clones were constructed for two isolates (LMV-0 and LMV-E) of Lettuce mosaic virus (LMV), a member of the genus Potyvirus. These two isolates differ in pathogenicity in susceptible and tolerant-resistant lettuce cultivars. In susceptible plants, LMV-0 induces mild mosaic symptoms, whereas LMV-E induces severe stunting, leaf deformation, and a necrotic mosaic. In plants carrying either of the two probably allelic recessive resistance genes mo11 or mo12, LMV-0 is restricted partially to the inoculated leaves. When a systemic invasion does occur, however, symptoms fail to develop. LMV-E overcomes the protection afforded by the resistance genes, resulting in systemic mosaic symptoms. Analysis of the behavior of recombinants constructed between the two virus isolates determined that the HC-Pro protein of LMV-E causes the severe stunting and necrotic mosaic induced by this isolate in susceptible cultivars. In contrast, the ability to overcome mo1 resistance and induce symptoms in the resistant-tolerant cultivars was mapped to the 3′ half of the LMV-E genome. These results indicate that the ability to induce severe symptoms and to overcome the protection afforded by the recessive genes mo11 or mo12 are independent phenomena.

Nucleotide sequence of the 3′ terminal region of the genome of four Lettuce mosaic virus isolates from Greece and Yemen

1999 ◽  
Vol 144 (8) ◽  
pp. 1619-1626 ◽  
Author(s):  
F. Revers ◽  
R. A. A. van der Vlugt ◽  
S. Souche ◽  
M. Lanneau ◽  
H. Lot ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Terminal Region ◽  
Lettuce Mosaic Virus ◽  
Virus Isolates

scholarly journals The Use of Green Fluorescent Protein-Tagged Recombinant Viruses to Test Lettuce mosaic virus Resistance in Lettuce

2002 ◽  
Vol 92 (2) ◽  
pp. 169-176 ◽  
Author(s):  
T. Candresse ◽  
O. Le Gall ◽  
B. Maisonneuve ◽  
S. German-Retana ◽  
E. Redondo
Keyword(s):  
Green Fluorescent Protein ◽  
Mosaic Virus ◽  
Fluorescent Protein ◽  
Lettuce Mosaic Virus ◽  
Recombinant Viruses ◽  
Systemic Movement ◽  
Resistance Breaking ◽  
Recessive Genes ◽  
Lettuce Breeding ◽  
Green Fluorescent

Seed certification and the use of cultivars containing one of two, probably allelic, recessive genes, mo11 and mo12, are the principal control methods for Lettuce mosaic virus (LMV) in lettuce. Although for a few LMV isolates, mo12 confers resistance with most isolates, the genes mo11 or mo12 confer a tolerance, and virus accumulation is readily detected in mo1-carrying plants. This phenotype complicates evaluation of the resistance status, in particular for mo11, for which there are no viral strains against which a true resistance is expressed. Two green fluorescent protein (GFP)-tagged viruses were constructed, derived from a non-resistance breaking isolate (LMV-0) and from a resistance-breaking isolate (LMV-E). An evaluation of 101 cultivars of known status was carried out with these recombinant viruses. Using the LMV-0-derived recombinant, identification of mo1-carrying cultivars was simple because, contrary to its wild-type parent, systemic movement of LMV-0-GFP was abolished in resistant plants. This assay detected four cases of misidentification of resistance status. In all these cases, further tests confirmed that the prior resistance status information was incorrect, so that a 100% correlation was observed between LMV-0-GFP behavior and the mo1 resistance status. Similarly, the LMV-E-derived recombinant allowed the identification of mo12 lettuce lines because its systemic movement was restricted in mo12 lines but not in susceptible or in mo11 lines. The tagged viruses were able to systemically invade another host, pea, irrespective of its resistance status against another member of the genus Potyvirus, Pea seed-borne mosaic virus. The use of these recombinant viruses could therefore greatly facilitate LMV resistance evaluation and speed up lettuce breeding programs.

Identification of PCR-based markers flanking the recessive LMV resistance gene mo1 in an intraspecific cross in lettuce

Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 982-986 ◽  
Author(s):  
S V Irwin ◽  
R V Kesseli ◽  
W Waycott ◽  
E J Ryder ◽  
J J Cho ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Disease Resistance Gene ◽  
Symptom Development ◽  
Recessive Resistance ◽  
Lettuce Mosaic Virus ◽  
Recessive Resistance Gene ◽  
The Common

Molecular markers flanking the recessive resistance gene mo1 were identified following analysis of two segregating populations. Generation of a population from a crisphead × crisphead cross of Lactuca sativa that segregated for resistance conferred by the mo12 allele, but not morphological traits, was required for accurate mapping of the gene. Resistance was best assessed by symptom development after inoculating F3 families with the common pathotype II isolate of lettuce mosaic virus (LMV). Bulked segregant analysis for RAPD (random amplified polymorphic DNA) markers using one population, followed by linkage analysis using another, identified markers on either side of the mo1 gene, approximately 8 cM apart. These markers will be useful for more efficient introgression of this resistance gene into additional lettuce cultivars.Key words: lettuce, lettuce mosaic virus, molecular marker, disease resistance gene, bulked segregant analysis.

Molecular mapping of novel resistance genes against Barley Mild Mosaic Virus (BaMMV)

1997 ◽  
Vol 95 (8) ◽  
pp. 1263-1269 ◽  
Author(s):  
E. Bauer ◽  
J. Weyen ◽  
A. Schiemann ◽  
A. Graner ◽  
F. Ordon
Keyword(s):  
Mosaic Virus ◽  
Resistance Genes ◽  
Molecular Mapping ◽  
Mild Mosaic ◽  
Mild Mosaic Virus

Molecular Detection and Characterization of Chinese Yam Mild Mosaic Virus Isolates

2014 ◽  
Vol 163 (11-12) ◽  
pp. 1036-1040 ◽  
Author(s):  
Mingqiang Wang ◽  
Fan Li ◽  
Guohui Zhou ◽  
Pingxiu Lan ◽  
Donglin Xu ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Molecular Detection ◽  
Mild Mosaic ◽  
Chinese Yam ◽  
Mild Mosaic Virus ◽  
Virus Isolates

Identification of Barley mild mosaic virus Isolates in Germany Breaking rym5 Resistance

2007 ◽  
Vol 0 (0) ◽  
pp. 071203163622002-???
Author(s):  
A. Habekuss ◽  
T. Kühne ◽  
I. Krämer ◽  
F. Rabenstein ◽  
F. Ehrig ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Mild Mosaic ◽  
Mild Mosaic Virus ◽  
Virus Isolates

scholarly journals Quantitative control of Lettuce mosaic virus fitness and host defence inhibition by P1-HCPro

2007 ◽  
Vol 33 (2) ◽  
pp. 119-123
Author(s):  
Renate Krause-Sakate ◽  
Florence Richard-Forget ◽  
Elise Redondo ◽  
Marcelo Agenor Pavan ◽  
Francisco Murilo Zerbini ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Viral Genome ◽  
Host Defence ◽  
Post Inoculation ◽  
Lettuce Mosaic Virus ◽  
European Isolate ◽  
Recombinant Viruses ◽  
Virus Isolates ◽  
Virus Fitness

Two Lettuce mosaic virus isolates capable of overcoming the resistance afforded by the resistance gene mo1² in lettuce, LMV-AF199 from Brazil, and LMV-E, an European isolate, were evaluated for the rapidity and severity of symptoms induced on the lettuce variety Salinas 88 (mo1²). The mosaic symptoms on Salinas 88 plants inoculated with LMV-AF199 appeared 7 days post-inoculation (dpi) and 15 dpi for LMV-E. The symptoms induced by LMV-AF199 in this cultivar were also more severe than those induced by LMV-E. In order to identify the region of the viral genome responsible for this phenotype, recombinant viruses were constructed between these isolates and the phenotype of each recombinant was analysed. The region encoding proteins P1 and HcPro from LMV-AF199 was associated with the increased virulence in Salinas 88.

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