scholarly journals Activity of Class III Peroxidases in the Defense of Cotton to Bacterial Blight

2003 ◽  
Vol 16 (11) ◽  
pp. 1030-1038 ◽  
Author(s):  
E. Delannoy ◽  
A. Jalloul ◽  
K. Assigbetsé ◽  
P. Marmey ◽  
J. P. Geiger ◽  
...  
Keyword(s):  
Oxidative Burst ◽  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Time Course ◽  
Hypersensitive Reaction ◽  
Guaiacol Peroxidase ◽  
Class Iii ◽  
Transcript Accumulation ◽  
Class Iii Peroxidases

Cotton cotyledons displayed a hypersensitive reaction (HR) in the cultivar Réba B50 after infiltration with the aviru-lent race 18 from Xanthomonas campestris pv. malvacearum. Two sets of peroxidases were associated with the HR time course. Early but transient accumulation of peroxidase in material encapsulating the bacteria in intercellular areas was observed by immunocytochemistry at 3 h postinfection and coincided with the oxidative burst. Total guaiacol-peroxidase activity was highly increased in cells undergoing HR, from 12 h after treatment. Molecular characterization of seven cloned peroxidase genes revealed highly conserved B, D, and F domains, with similarities to plant class III peroxidases. Analysis of gene expression showed variation in transcript accumulation during both compatible (race 20) and incompatible interactions for four of these genes: pod2, pod3, pod4, and pod6.Pod4 and pod6 were more intensely up-regulated during resistance than during disease and in the control, while pod3 was specifically down-regulated during the HR after the oxidative burst. Pod2 was induced by pathogen infection and weakly stimulated in the control. These data suggest that cotton peroxidases may have various functions in the defense response to Xanthomonas infections.

scholarly journals Characterization of Xanthomonas axonopodis pv. phaseoli isolates

2008 ◽  
Vol 34 (3) ◽  
pp. 228-231 ◽  
Author(s):  
Willian Mário de Carvalho Nunes ◽  
Maria Júlia Corazza ◽  
Silvana Aparecida Crestes Dias de Souza ◽  
Siu Mui Tsai ◽  
Eiko Eurya Kuramae
Keyword(s):  
Xanthomonas Campestris ◽  
Random Amplified Polymorphic Dna ◽  
Hypersensitive Reaction ◽  
Chain Reaction ◽  
Xanthomonas Axonopodis ◽  
Paulo State ◽  
Total Dna ◽  
Polymerase Chain ◽  
Bacterial Plant Pathogen

A simple, quick and easy protocol was standardized for extraction of total DNA of the bacteria Xanthomonas axonopodis pv. phaseoli. The DNA obtained by this method had high quality and the quantity was enough for the Random Amplified Polymorphic DNA (RAPD) reactions with random primers, and Polymerase Chain Reaction (PCR) with primers of the hypersensitivity and pathogenicity gene (hrp). The DNA obtained was free of contamination by proteins or carbohydrates. The ratio 260nm/380nm of the DNA extracted ranged from 1.7 to 1.8. The hrp gene cluster is required by bacterial plant pathogen to produce symptoms on susceptible hosts and hypersensitive reaction on resistant hosts. This gene has been found in different bacteria as well as in Xanthomonas campestris pv. vesicatoria (9). The primers RST21 and RST22 (9) were used to amplify the hrp gene of nine different isolates of Xanthomonas axonopodis pv. phaseoli from Botucatu, São Paulo State, Brazil, and one isolate, "Davis". PCR amplified products were obtained in all isolates pathogenic to beans.

Suspension cell culture as a tool for the characterization of class III peroxidases in sugarcane

2013 ◽  
Vol 62 ◽  
pp. 1-10 ◽  
Author(s):  
Igor Cesarino ◽  
Pedro Araújo ◽  
Adriana Franco Paes Leme ◽  
Silvana Creste ◽  
Paulo Mazzafera
Keyword(s):  
Cell Culture ◽  
Suspension Cell ◽  
Suspension Cell Culture ◽  
Class Iii ◽  
Class Iii Peroxidases

scholarly journals Characterization of Class III Peroxidases from Switchgrass

2016 ◽  
Vol 173 (1) ◽  
pp. 417-433 ◽  
Author(s):  
Timothy W. Moural ◽  
Kevin M. Lewis ◽  
Carlo Barnaba ◽  
Fang Zhu ◽  
Nathan A Palmer ◽  
...  
Keyword(s):  
Class Iii ◽  
Class Iii Peroxidases

scholarly journals Cloning and Characterization of a Bean Udp-Glucosyltransferase cDNA Expressed During Plant-Bacterial Interactions

2001 ◽  
Vol 14 (1) ◽  
pp. 90-92 ◽  
Author(s):  
T. A. Sullivan ◽  
J. L. Jakobek ◽  
P. B. Lindgren
Keyword(s):  
Pseudomonas Syringae ◽  
Cdna Clone ◽  
Hypersensitive Reaction ◽  
Northern Analysis ◽  
Transcript Accumulation ◽  
Bacterial Interactions ◽  
Rna Transcript

A cDNA clone, which corresponds to an RNA transcript that accumulates in bean during the hypersensitive reaction, was isolated and designated Hra25 (for hypersensitive reaction associated). Hra25 is predicted to encode a UDP-glucosyltransferase. Northern analysis was used to study Hra25 transcript accumulation in bean in response to incompatible and compatible strains of Pseudomonas syringae, an Hrp¯ mutant, and wounding. Our data suggest that the Hra25 transcript is activated in response to specific avr-derived signals as well as non-avr, general signals.

scholarly journals Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes

2020 ◽  
Vol 21 (22) ◽  
pp. 8872
Author(s):  
Anne Hofmann ◽  
Stefanie Wienkoop ◽  
Sönke Harder ◽  
Fabian Bartlog ◽  
Sabine Lüthje
Keyword(s):  
Cross Sections ◽  
Phenylpropanoid Pathway ◽  
Guaiacol Peroxidase ◽  
Class Iii ◽  
Low Oxygen ◽  
Aerenchyma Formation ◽  
2D Page ◽  
Membrane Bound ◽  
Class Iii Peroxidases ◽  
Scavenging Enzymes

Flooding induces low-oxygen environments (hypoxia or anoxia) that lead to energy disruption and an imbalance of reactive oxygen species (ROS) production and scavenging enzymes in plants. The influence of hypoxia on roots of hydroponically grown maize (Zea mays L.) plants was investigated. Gene expression (RNA Seq and RT-qPCR) and proteome (LC–MS/MS and 2D-PAGE) analyses were used to determine the alterations in soluble and membrane-bound class III peroxidases under hypoxia. Gel-free peroxidase analyses of plasma membrane-bound proteins showed an increased abundance of ZmPrx03, ZmPrx24, ZmPrx81, and ZmPr85 in stressed samples. Furthermore, RT-qPCR analyses of the corresponding peroxidase genes revealed an increased expression. These peroxidases could be separated with 2D-PAGE and identified by mass spectrometry. An increased abundance of ZmPrx03 and ZmPrx85 was determined. Further peroxidases were identified in detergent-insoluble membranes. Co-regulation with a respiratory burst oxidase homolog (Rboh) and key enzymes of the phenylpropanoid pathway indicates a function of the peroxidases in membrane protection, aerenchyma formation, and cell wall remodeling under hypoxia. This hypothesis was supported by the following: (i) an elevated level of hydrogen peroxide and aerenchyma formation; (ii) an increased guaiacol peroxidase activity in membrane fractions of stressed samples, whereas a decrease was observed in soluble fractions; and (iii) alterations in lignified cells, cellulose, and suberin in root cross-sections.

scholarly journals The Dual Effect of the Brassinosteroid Pathway on Rice Black-Streaked Dwarf Virus Infection by Modulating the Peroxidase-Mediated Oxidative Burst and Plant Defense

2019 ◽  
Vol 32 (6) ◽  
pp. 685-696 ◽  
Author(s):  
Hehong Zhang ◽  
Yuqing He ◽  
Xiaoxiang Tan ◽  
Kaili Xie ◽  
Lulu Li ◽  
...  
Keyword(s):  
Plant Defense ◽  
Oxidative Burst ◽  
High Throughput Sequencing ◽  
Dwarf Virus ◽  
Class Iii ◽  
Defense System ◽  
Plant Growth And Development ◽  
Dual Effect ◽  
Ros Burst ◽  
Class Iii Peroxidases

The phytohormone brassinosteroid (BR) not only plays key roles in regulating plant growth and development but is also involved in modulating the plant defense system in response to pathogens. We previously found that BR application made rice plants more susceptible to the devastating pathogen rice black-streaked dwarf virus (RBSDV), but the mechanism of BR-mediated susceptibility remains unclear. We now show that both BR-deficient and -insensitive mutants are resistant to RBSDV infection. High-throughput sequencing showed that the defense hormone salicylic acid and jasmonic acid pathways were activated in the RBSDV-infected BR mutant. Meanwhile, a number of class III peroxidases (OsPrx) were significantly changed and basal reactive oxygen species (ROS) accumulated in BR mutants. Treatment with exogenous hormones and other chemicals demonstrated that the BR pathway could suppress the levels of OsPrx and the ROS burst by directly binding the promoters of OsPrx genes. Together, our findings indicate that BR-mediated susceptibility is at least partly caused by inhibition of the action of defense hormones, preventing the accumulation of the peroxidase-mediated oxidative burst.

scholarly journals Four Near-Isogenic Lines of Cotton with Different Genes for Bacterial Blight Resistance

2002 ◽  
Vol 92 (12) ◽  
pp. 1323-1328 ◽  
Author(s):  
Margaret Essenberg ◽  
Melanie B. Bayles ◽  
Roushan A. Samad ◽  
Judy A. Hall ◽  
L. A. Brinkerhoff ◽  
...  
Keyword(s):  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Avirulence Gene ◽  
Recurrent Parent ◽  
Near Isogenic Lines ◽  
Isogenic Lines ◽  
Race 1 ◽  
Selection For ◽  
Dominant Genes

The development and genetic characterization of four near-isogenic lines (NILs) of cotton (Gossypium hirsutum) is described herein. Each line contains a single, but different, gene for resistance to bacterial blight caused by Xanthomonas campestris pv. malvacearum. The lines were derived using at least six backcrosses to the susceptible recurrent parent ‘Acala 44’, followed by single plant-progeny row selection for uniformity. The NILs are homozygous for the B2, B4, BIn, or b7 genes and are designated as AcB2, AcB4, AcBIn, and Acb7, respectively. In the ‘Acala 44’ background, B2, B4, and BIn are partially dominant genes; b7 is partially recessive. Relative strengths of resistance conferred by those genes toward race 1 of the pathogen were B4˜b7>BIn˜B2. B4, BIn, and b7 each conferred resistance toward X. campestris pv. malvacearum carrying a single avirulence gene, whereas B2 was less specific.

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