scholarly journals Characterization of Class III Peroxidases from Switchgrass

2016 ◽  
Vol 173 (1) ◽  
pp. 417-433 ◽  
Author(s):  
Timothy W. Moural ◽  
Kevin M. Lewis ◽  
Carlo Barnaba ◽  
Fang Zhu ◽  
Nathan A Palmer ◽  
...  
Keyword(s):  
Class Iii ◽  
Class Iii Peroxidases

Suspension cell culture as a tool for the characterization of class III peroxidases in sugarcane

2013 ◽  
Vol 62 ◽  
pp. 1-10 ◽  
Author(s):  
Igor Cesarino ◽  
Pedro Araújo ◽  
Adriana Franco Paes Leme ◽  
Silvana Creste ◽  
Paulo Mazzafera
Keyword(s):  
Cell Culture ◽  
Suspension Cell ◽  
Suspension Cell Culture ◽  
Class Iii ◽  
Class Iii Peroxidases

scholarly journals Activity of Class III Peroxidases in the Defense of Cotton to Bacterial Blight

2003 ◽  
Vol 16 (11) ◽  
pp. 1030-1038 ◽  
Author(s):  
E. Delannoy ◽  
A. Jalloul ◽  
K. Assigbetsé ◽  
P. Marmey ◽  
J. P. Geiger ◽  
...  
Keyword(s):  
Oxidative Burst ◽  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Time Course ◽  
Hypersensitive Reaction ◽  
Guaiacol Peroxidase ◽  
Class Iii ◽  
Transcript Accumulation ◽  
Class Iii Peroxidases

Cotton cotyledons displayed a hypersensitive reaction (HR) in the cultivar Réba B50 after infiltration with the aviru-lent race 18 from Xanthomonas campestris pv. malvacearum. Two sets of peroxidases were associated with the HR time course. Early but transient accumulation of peroxidase in material encapsulating the bacteria in intercellular areas was observed by immunocytochemistry at 3 h postinfection and coincided with the oxidative burst. Total guaiacol-peroxidase activity was highly increased in cells undergoing HR, from 12 h after treatment. Molecular characterization of seven cloned peroxidase genes revealed highly conserved B, D, and F domains, with similarities to plant class III peroxidases. Analysis of gene expression showed variation in transcript accumulation during both compatible (race 20) and incompatible interactions for four of these genes: pod2, pod3, pod4, and pod6.Pod4 and pod6 were more intensely up-regulated during resistance than during disease and in the control, while pod3 was specifically down-regulated during the HR after the oxidative burst. Pod2 was induced by pathogen infection and weakly stimulated in the control. These data suggest that cotton peroxidases may have various functions in the defense response to Xanthomonas infections.

scholarly journals Genome-wide identification and analysis of class III peroxidases in Betula pendula

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Kewei Cai ◽  
Huixin Liu ◽  
Song Chen ◽  
Yi Liu ◽  
Xiyang Zhao ◽  
...  
Keyword(s):  
Stress Responses ◽  
Betula Pendula ◽  
Expression Patterns ◽  
Class Iii ◽  
Physiological Processes ◽  
Plant Life ◽  
Genome Wide ◽  
Plant Life Cycle ◽  
Class Iii Peroxidases ◽  
And Function

Abstract Background Class III peroxidases (POD) proteins are widely present in the plant kingdom that are involved in a broad range of physiological processes including stress responses and lignin polymerization throughout the plant life cycle. At present, POD genes have been studied in Arabidopsis, rice, poplar, maize and Chinese pear, but there are no reports on the identification and function of POD gene family in Betula pendula. Results We identified 90 nonredundant POD genes in Betula pendula. (designated BpPODs). According to phylogenetic relationships, these POD genes were classified into 12 groups. The BpPODs are distributed in different numbers on the 14 chromosomes, and some BpPODs were located sequentially in tandem on chromosomes. In addition, we analyzed the conserved domains of BpPOD proteins and found that they contain highly conserved motifs. We also investigated their expression patterns in different tissues, the results showed that some BpPODs might play an important role in xylem, leaf, root and flower. Furthermore, under low temperature conditions, some BpPODs showed different expression patterns at different times. Conclusions The research on the structure and function of the POD genes in Betula pendula plays a very important role in understanding the growth and development process and the molecular mechanism of stress resistance. These results lay the theoretical foundation for the genetic improvement of Betula pendula.

scholarly journals The G-Protein α-Subunit GasC Plays a Major Role in Germination in the Dimorphic FungusPenicillium marneffei

Genetics ◽  
2003 ◽  
Vol 164 (2) ◽  
pp. 487-499 ◽  
Author(s):  
Sophie Zuber ◽  
Michael J Hynes ◽  
Alex Andrianopoulos
Keyword(s):  
G Protein ◽  
Germination Rate ◽  
Yeast Cells ◽  
Class Iii ◽  
Temperature Dependent ◽  
Gene Encoding ◽  
Α Subunit ◽  
G Protein Α Subunit ◽  
Opportunistic Human Pathogen

AbstractThe opportunistic human pathogen Penicillium marneffei exhibits a temperature-dependent dimorphic switch. At 25°, multinucleate, septate hyphae that can undergo differentiation to produce asexual spores (conidia) are produced. At 37° hyphae undergo arthroconidiation to produce uninucleate yeast cells that divide by fission. This work describes the cloning of the P. marneffei gasC gene encoding a G-protein α-subunit that shows high homology to members of the class III fungal Gα-subunits. Characterization of a ΔgasC mutant and strains carrying a dominant-activating gasCG45R or a dominant-interfering gasCG207R allele show that GasC is a crucial regulator of germination. A ΔgasC mutant is severely delayed in germination, whereas strains carrying a dominant-activating gasCG45R allele show a significantly accelerated germination rate. Additionally, GasC signaling positively affects the production of the red pigment by P. marneffei at 25° and negatively affects the onset of conidiation and the conidial yield, showing that GasC function overlaps with functions of the previously described Gα-subunit GasA. In contrast to the S. cerevisiae ortholog Gpa2, our data indicate that GasC is not involved in carbon or nitrogen source sensing and plays no major role in either hyphal or yeast growth or in the switch between these two forms.

scholarly journals Characterization of VPS34-IN1, a selective inhibitor of Vps34, reveals that the phosphatidylinositol 3-phosphate-binding SGK3 protein kinase is a downstream target of class III phosphoinositide 3-kinase

2014 ◽  
Vol 463 (3) ◽  
pp. 413-427 ◽  
Author(s):  
Ruzica Bago ◽  
Nazma Malik ◽  
Michael J. Munson ◽  
Alan R. Prescott ◽  
Paul Davies ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Selective Inhibitor ◽  
Class Iii ◽  
Phosphate Binding ◽  
Downstream Target ◽  
Phosphoinositide 3 Kinase ◽  
Phosphatidylinositol 3

We characterize VPS34-IN, a potent and selective inhibitor of class III Vps34 PI3K. Using VPS34-IN1, we demonstrate that PtdIns(3)P, produced by Vps34 controls phosphorylation and activity of the SGK3 protein kinase.

scholarly journals Enzyme-catalyzed Mechanism of Isoniazid Activation in Class I and Class III Peroxidases

2004 ◽  
Vol 279 (37) ◽  
pp. 39000-39009 ◽  
Author(s):  
Roberta Pierattelli ◽  
Lucia Banci ◽  
Nigel A. J. Eady ◽  
Jacques Bodiguel ◽  
Jamie N. Jones ◽  
...  
Keyword(s):  
Class I ◽  
Class Iii ◽  
Class Iii Peroxidases

scholarly journals Transcriptome-wide Identification and Characterization of microRNAs and Their Targets in a Highly Adaptable Conifer Platycladus orientalis

2022 ◽  
Vol 147 (1) ◽  
pp. 7-17
Author(s):  
Ying Yang ◽  
Xian-Ge Hu ◽  
Bingsong Zheng ◽  
Yue Li ◽  
Tongli Wang ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Leucine Zipper ◽  
Class Iii ◽  
Conifer Species ◽  
Essential Information ◽  
Sequencing Platform ◽  
Platycladus Orientalis ◽  
Wide Range ◽  
Identification And Characterization

MicroRNAs (miRNAs) are short noncoding RNAs (20–25 nucleotides) that regulate gene expression posttranscriptionally. However, identification and characterization of miRNAs remain limited for conifer species. In this study, we applied transcriptome-wide miRNAs sequencing to a conifer species Platycladus orientalis, which is highly adaptable to a wide range of environmental adversities, including drought, barren soil, and mild salinity. A total of 17,181,542 raw reads were obtained from the Illumina sequencing platform; 31 conserved and 91 novel miRNAs were identified, and their unique characteristics were further analyzed. Ten randomly selected miRNAs were validated by quantificational real-time polymerase chain reaction. Through miRNA target predictions based on psRNATarget, 2331 unique mRNAs were predicted to be targets of P. orientalis miRNAs that involved in 187 metabolic pathways in KEGG database. These targets included not only important transcription factors (e.g., class III homeodomain leucine zipper targeted by por-miR166d) but also indispensable nontranscriptional factor proteins (i.e., por-miR482a-3p regulated nucleotide-binding site leucine-rich repeat protein). Interestingly, six miRNAs (por-miR16, -miR44, -miR60-5p, -miR69–3p, -miR166b-5p, and -miR395c) were found in adaptation-related pathways (e.g., drought), indicating their possible involved in this species’ stress-tolerance characteristics. The present study provided essential information for understanding the regulatory role of miRNAs in P. orientalis and sheds light on their possible use in tree improvement for stress tolerance.

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