Pathogenicity and Distribution of Fusarium solani Isolates Associated with Erythrina Decline in Japan

Erythrina spp. trees have been declining since the 2000s worldwide, and fungi belonging to Fusarium solani species complex (FSSC) have been suggested to be a causal factor of decline and mortality of Erythrina variegata trees in Okinawa Island, Japan. In addition to the FSSC isolate grouped as “it-1” based on ITS sequence data (previously called strain A), we conducted an inoculation experiment with two isolates grouped as “it-2” (previously strain B), which is genetically close to it-1. Two it-2 isolates originating from two islands showed pathogenicity to E. variegata with the same symptoms as those caused by it-1 isolate. We also found the isolates of it-1 and it-2 were widely distributed, including on Ishigaki Island, ∼400 km south of Okinawa Island across the ocean. All isolates of it-1 and it-2 belong to the ambrosia Fusarium clade of the FSSC, a group of symbionts of ambrosia beetles, including the pathogens of Fusarium dieback in avocados and teas. The detection of ambrosia beetles Euwallacea spp. from our specimens provided information on the vectors of the pathogens. Our present results suggest the fungi of the FSSC could be responsible for the Erythrina decline in other areas with damage.

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Molecular phylogeny of the Fusarium solani species complex (FSSC) isolated from soils in Iran

Botany ◽

10.1139/cjb-2014-0096 ◽

2014 ◽

Vol 92 (11) ◽

pp. 815-820 ◽

Cited By ~ 2

Author(s):

Khosrow Chehri

Keyword(s):

Dna Sequences ◽

Fusarium Solani ◽

Species Complex ◽

Agricultural Soils ◽

Sequence Data ◽

Morphological Characteristics ◽

Taxonomic Status ◽

Its Region ◽

Phylogenetic Study ◽

Fusarium Solani Species Complex

Members of Fusarium solani species complex (FSSC) are frequently isolated from soils, food, feeds, trees, and to some extent from humans and other animals. The taxonomic status of these fungi is being revised but no attempt has been made to identify those isolated in Iran, a mountainous country with a high biodiversity. The objective of the present research was to study the phylogenetic diversity of FSSC strains recovered from soils in Iran by analyzing morphological characteristics and DNA sequences. A total of 65 strains belonging to the FSSC were recovered from agricultural soils in western Iran. Based on differences in their morphological characters, 25 strains were selected for phylogenetic analysis employing translation elongation factor-1α (tef1) and internal transcribed spacer (ITS) region sequences. Comparisons of DNA sequence data revealed that all isolates belonged to Fusarium falciforme, Fusarium keratoplasticum, Fusarium petroliphilum, the unnamed species FSSC 5, and unknown species of Fusarium, which represents a new lineage within members of Clade 3. Based on morphological features and phylogenetic study, F. keratoplasticum and F. petroliphilum were reported for the first time in Iran.

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First report of Fusarium solani species complex as a causal agent of Erythrina variegata decline and death after gall formation by Quadrastichus erythrinae on Okinawa Island, Japan

Journal of General Plant Pathology ◽

10.1007/s10327-017-0738-3 ◽

2017 ◽

Vol 83 (6) ◽

pp. 344-357 ◽

Cited By ~ 1

Author(s):

Keiko Kuroda ◽

Izumi Chuma ◽

Takeo Kihara ◽

Tsubasa Murakami ◽

Kuya Takashina ◽

...

Keyword(s):

Fusarium Solani ◽

Species Complex ◽

Causal Agent ◽

Gall Formation ◽

Okinawa Island ◽

First Report ◽

Fusarium Solani Species Complex ◽

Erythrina Variegata

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Morphology, pathogenicity and molecular identification of Fusarium solani species complex (FSSC) associated with potato tubers

10.21203/rs.3.rs-147809/v1 ◽

2021 ◽

Author(s):

Youssuf Gherbawy ◽

Mohamed Hussein ◽

Nabila Hassany ◽

Yassmin Shebany ◽

Mohamed Awad ◽

...

Keyword(s):

Fusarium Solani ◽

Species Complex ◽

Sequence Data ◽

Solanum Tuberosum L ◽

Elongation Factor ◽

Molecular Characteristics ◽

Potato Tubers ◽

Dry Rot ◽

Upper Egypt ◽

Fusarium Solani Species Complex

Abstract Potato (Solanum tuberosum L.) is among the top five crops growing worldwide following cereals, wheat, rice, corn and barley due to its high carbohydrate content and adaptability. Potatoes are particularly valued in developing countries as a rich source of starch, vitamins C and B6 and essential amino acids. Fusarium solani species complex (FSSC) is common pathogen of potato, causing dry rot in the Upper Egypt. In this study were isolated and identified FSSC from potato tubers based on the morphological and molecular characteristics. 187 isolates of Fusarium solani were obtained from potato tubers collected from different regions in the Upper Egypt. Based on the morphological characters, sequence data from β-tubulin and translation elongation factor (TEF-1α) genes, all of the selected FSSC isolates were divided into three major groups (F. keratoplasticum, F. falciforme and F. solani). All the tested FSSC were able to produce amylases. All of the isolates were evaluated for their pathogenicity on healthy potato tubers; which showed pathogenic effect, lesion sizes were quite variable. F. solani (SVUFs73) had a highly virulent effect.

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