Morphology, pathogenicity and molecular identification of Fusarium solani species complex (FSSC) associated with potato tubers

Abstract Potato (Solanum tuberosum L.) is among the top five crops growing worldwide following cereals, wheat, rice, corn and barley due to its high carbohydrate content and adaptability. Potatoes are particularly valued in developing countries as a rich source of starch, vitamins C and B6 and essential amino acids. Fusarium solani species complex (FSSC) is common pathogen of potato, causing dry rot in the Upper Egypt. In this study were isolated and identified FSSC from potato tubers based on the morphological and molecular characteristics. 187 isolates of Fusarium solani were obtained from potato tubers collected from different regions in the Upper Egypt. Based on the morphological characters, sequence data from β-tubulin and translation elongation factor (TEF-1α) genes, all of the selected FSSC isolates were divided into three major groups (F. keratoplasticum, F. falciforme and F. solani). All the tested FSSC were able to produce amylases. All of the isolates were evaluated for their pathogenicity on healthy potato tubers; which showed pathogenic effect, lesion sizes were quite variable. F. solani (SVUFs73) had a highly virulent effect.

Download Full-text

Morphology, pathogenicity, and molecular identification of Fusarium solani species complex (FSSC) associated with potato tubers

10.21203/rs.3.rs-152393/v1 ◽

2021 ◽

Author(s):

Youssuf A. Gherbawy ◽

Mohamed A. Hussein ◽

Nabila A. Hassany ◽

Mohamed F. Awad ◽

Yassmin M. Shebany ◽

...

Keyword(s):

Fusarium Solani ◽

Species Complex ◽

Sequence Data ◽

Elongation Factor ◽

Morphological Characteristics ◽

Essential Amino Acids ◽

Molecular Characteristics ◽

Potato Tubers ◽

Dry Rot ◽

Upper Egypt

Abstract Potato (Solanum tuberosum L.) is among the top five crops growing worldwide following cereals, rice, wheat, barley and corn due to its high carbohydrate content and adaptability. Potatoes are particularly valued in developing countries as a rich source of vitamins C and B6, starch and essential amino acids. Fusarium solani species complex (FSSC) is a common pathogen of potato, causing dry rot in the Upper Egypt. In this study were isolated and identified FSSC from potato tubers based on the morphological followed by molecular characteristics. 187 isolates of Fusarium solani were achieved from infected and non-infected potato tubers gathered from different markets in the Upper Egypt. Dependent upon the morphological characteristics, sequence data from amplifying β-tubulin and specific translation elongation factor (TEF-1α) genes, all of the selected FSSC isolates were divided into three major groups (F. keratoplasticum, F. falciforme and F. solani). All the tested FSSC were capable of producing amylases. All of the isolates were examined for their pathogenic ability on healthy potato tubers, which showed pathogenic effects; with lesion sizes were quite variable. F. solani SVUFs73 showed a highly virulent effect.

Download Full-text

Molecular phylogeny of the Fusarium solani species complex (FSSC) isolated from soils in Iran

Botany ◽

10.1139/cjb-2014-0096 ◽

2014 ◽

Vol 92 (11) ◽

pp. 815-820 ◽

Cited By ~ 2

Author(s):

Khosrow Chehri

Keyword(s):

Dna Sequences ◽

Fusarium Solani ◽

Species Complex ◽

Agricultural Soils ◽

Sequence Data ◽

Morphological Characteristics ◽

Taxonomic Status ◽

Its Region ◽

Phylogenetic Study ◽

Fusarium Solani Species Complex

Members of Fusarium solani species complex (FSSC) are frequently isolated from soils, food, feeds, trees, and to some extent from humans and other animals. The taxonomic status of these fungi is being revised but no attempt has been made to identify those isolated in Iran, a mountainous country with a high biodiversity. The objective of the present research was to study the phylogenetic diversity of FSSC strains recovered from soils in Iran by analyzing morphological characteristics and DNA sequences. A total of 65 strains belonging to the FSSC were recovered from agricultural soils in western Iran. Based on differences in their morphological characters, 25 strains were selected for phylogenetic analysis employing translation elongation factor-1α (tef1) and internal transcribed spacer (ITS) region sequences. Comparisons of DNA sequence data revealed that all isolates belonged to Fusarium falciforme, Fusarium keratoplasticum, Fusarium petroliphilum, the unnamed species FSSC 5, and unknown species of Fusarium, which represents a new lineage within members of Clade 3. Based on morphological features and phylogenetic study, F. keratoplasticum and F. petroliphilum were reported for the first time in Iran.

Download Full-text

Pathogenicity and Distribution of Fusarium solani Isolates Associated with Erythrina Decline in Japan

Plant Disease ◽

10.1094/pdis-01-19-0044-re ◽

2020 ◽

Vol 104 (3) ◽

pp. 731-742

Author(s):

Kuya Takashina ◽

Izumi Chuma ◽

Hisashi Kajimura ◽

Norikazu Kameyama ◽

Chiaki Goto ◽

...

Keyword(s):

Fusarium Solani ◽

Species Complex ◽

Sequence Data ◽

Its Sequence ◽

Inoculation Experiment ◽

Ambrosia Beetles ◽

Okinawa Island ◽

Fusarium Solani Species Complex ◽

Ishigaki Island ◽

Fusarium Dieback

Erythrina spp. trees have been declining since the 2000s worldwide, and fungi belonging to Fusarium solani species complex (FSSC) have been suggested to be a causal factor of decline and mortality of Erythrina variegata trees in Okinawa Island, Japan. In addition to the FSSC isolate grouped as “it-1” based on ITS sequence data (previously called strain A), we conducted an inoculation experiment with two isolates grouped as “it-2” (previously strain B), which is genetically close to it-1. Two it-2 isolates originating from two islands showed pathogenicity to E. variegata with the same symptoms as those caused by it-1 isolate. We also found the isolates of it-1 and it-2 were widely distributed, including on Ishigaki Island, ∼400 km south of Okinawa Island across the ocean. All isolates of it-1 and it-2 belong to the ambrosia Fusarium clade of the FSSC, a group of symbionts of ambrosia beetles, including the pathogens of Fusarium dieback in avocados and teas. The detection of ambrosia beetles Euwallacea spp. from our specimens provided information on the vectors of the pathogens. Our present results suggest the fungi of the FSSC could be responsible for the Erythrina decline in other areas with damage.

Download Full-text

Phylogeny and pathogenicity of Fusarium solani species complex (FSSC) associated with potato tubers

Journal of Basic Microbiology ◽

10.1002/jobm.202100393 ◽

2021 ◽

Author(s):

Youssuf A. Gherbawy ◽

Mohamed A. Hussein ◽

Nabila A. Hassany ◽

Yassmin M. Shebany ◽

Sabry Hassan ◽

...

Keyword(s):

Fusarium Solani ◽

Species Complex ◽

Potato Tubers ◽

Fusarium Solani Species Complex

Download Full-text

Identification of Fusarium spp. Associated with Potato Tubers in Upper Egypt by Morphological and Molecular Characters

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2019/v2i330062 ◽

2019 ◽

pp. 1-14 ◽

Cited By ~ 1

Author(s):

Youssuf A. Gherbawy ◽

Mohamed A. Hussein ◽

Eman G. A. El-Dawy ◽

Nabila A. Hassany ◽

Saad A. Alamri

Keyword(s):

Solanum Tuberosum L ◽

Fusarium Species ◽

Correct Diagnosis ◽

Potato Tubers ◽

Fusarium Spp ◽

Dry Rot ◽

Upper Egypt ◽

Fusarium Dry Rot ◽

Global Population ◽

Highly Virulent

Potato (Solanum tuberosum L.) is an important crop which holds promise for food security considering the global population growth rate. Fusarium dry rot is one of the most significant diseases of potato. To build up strategies for the control of this disease, it ought to be made primarily a correct diagnosis and identification of the pathogen. A total of 504 Fusarium isolates were recovered from potato tubers collected from Upper Egypt. Fusarium isolates were identified based on morphology and partial DNA sequencing of β-tubulin (TUB) genes. 62.5% of the isolates were identified as F. sambucinum, followed F. oxysporum (57.5%), then F. verticillioides (56.25%) and F. incarnatum (47.5%). All the tested Fusarium species were able to produce amylase. The pathogenicity of the isolates was tested by inoculation of healthy potato tubers; all of the tested isolates were pathogenic to healthy potato tubers. F. sambucinum had a highly virulent effect.

Download Full-text