Presence and correlation of Fusarium graminearum and deoxynivalenol accumulation in silage corn plant parts

Plant Disease ◽  
2021 ◽  
Author(s):  
Hannah Reed ◽  
Brian Mueller ◽  
Carol Groves ◽  
Damon L. Smith
Keyword(s):  
Fusarium Graminearum ◽  
High Energy ◽  
Gibberella Zeae ◽  
Corn Silage ◽  
Plant Diseases ◽  
Corn Plant ◽  
Stalk Rot ◽  
Plant Parts ◽  
Ear Infections ◽  
Highly Correlated

Corn silage, made from Zea mays, is a high energy feed that is important for feeding dairy cows. Plant diseases, such as those caused by Fusarium graminearum , can decrease silage corn yields and quality. Fusarium graminearum (teleomorph Gibberella zeae) is an ascomycete fungus that causes Gibberella ear and stalk rot in corn. Fusarium graminearum produces deoxynivalenol (DON), a secondary metabolite toxic to humans and animals. An understanding of the distribution of DON and F. graminearum throughout the corn plant is important for determining the quality of corn silage. A partitioned sample experiment that included two brown-midrib silage hybrids and three fungicide treatments was conducted in research plots located in Arlington, Wisconsin in 2018 and 2019. At harvest, stalk and ear parts were physically separated, dried, and ground for analysis. DON concentration (ppm) was determined using ELISA and F. graminearum DNA concentration (pg/ng) was determined using qPCR. Both DON and F. graminearum DNA were detected in all samples, demonstrating accumulation of the fungus in both stalks and ears of the plant. In 2018, DON contamination was as high as 30 ppm and varied drastically between stalks and ears. In 2019, DON concentrations were much lower (<5ppm), but were consistently higher in stalk samples than ear samples. Across all samples DON concentrations and F. graminearum accumulation were highly correlated within the separated stalk (r=0.78) and ear portions (r=0.87) but were not correlated between ears and stalks. Depending on the weather and planting conditions in a given year, either stalk infections or ear infections may occur by F. graminearum leading to subsequent DON increases in those respective parts that are independent of each other.

THE SEVERITY OF STALK ROT OF GRAIN CORN IN RELATION TO INFESTATIONS OF A BIVOLTINE STRAIN OF THE EUROPEAN CORN BORER, OSTRINIA NUBILALIS (LEPIDOPTERA: PYRALIDAE)

1983 ◽  
Vol 115 (9) ◽  
pp. 1235-1237
Author(s):  
W. H. Foott ◽  
P. R. Timmins
Keyword(s):  
Zea Mays ◽  
Fusarium Graminearum ◽  
Ostrinia Nubilalis ◽  
European Corn Borer ◽  
Direct Relationship ◽  
Inbred Lines ◽  
Gibberella Zeae ◽  
Corn Borer ◽  
Stalk Rot ◽  
Lepidoptera Pyralidae

Corn lodging due to bent or broken stalks results mostly from feeding by the European corn borer, Ostrinia nubilalis (Hübner), and from stalk rot infections. Stalk rot of corn (Zea mays L.) is caused in southwestern Ontario mainly by Gibberella zeae (Schw.) Petch (Fusarium graminearum Schwabe), which attacks roots during the summer and invades the bases of the stems as the plants mature (Gates and Mortimore 1972). Several studies in the U.S.A. reported an association between the rot and the borer. Christensen and Schneider (1950) recorded severest stalk rot with highest borer infestation and found that inbred lines usually free from rot became susceptible when infested by the borer. Chiang and Wilcoxson (1961) reported a higher incidence of rot in plants artificially inoculated with Fusarium graminearum and borer larvae than in those inoculated with F. graminearum alone. A recent study in Iowa (Jarvis et al. 1982) showed that the greatest stalk rot damage occurred in high borer infestations. Carson and Hooker (1981), however, were unable to relate the incidence and severity of rot to corn borer infestation. In their 3-year study, the least rot occurred in the year of severest borer infestation. Since observations in corn fields in southwestern Ontario also indicated that there might not be a direct relationship between severity of stalk rot and severity of corn borer infestation, further studies were undertaken.

The relationship between strains of Fusarium graminearum schwabe causing crown rot of various gramineous hosts and stalk rot of maize in Queensland

1969 ◽  
Vol 20 (2) ◽  
pp. 257 ◽  
Author(s):  
GS Purss
Keyword(s):  
Fusarium Graminearum ◽  
Root Rot ◽  
Gibberella Zeae ◽  
Crown Rot ◽  
Perfect State ◽  
Stalk Rot ◽  
Canary Grass ◽  
Pathogenic Races ◽  
The Relationship ◽  
Phalaris Canariensis

The fungus Fusarium graminearum is shown to have a wide host range on gramineous plants in Queensland. Isolates from hosts other than maize when inoculated onto wheat seed were capable of causing the disease known as crown rot in resulting plants. The same disease was reproduced by wheat isolates in other gramineous hosts including canary grass (Phalaris canariensis), barley, and rye but oats was only slightly susceptible. No symptoms typical of field infection could be reproduced in maize. Root rot occurred in linseed. The perfect state of the organism as it occurs in Queensland is described and identified as Gibberella zeae. This was reproduced experimentally, easily in the case of maize isolates but only with some difficulty with isolates from other hosts. The occurrence in Queensland of pathogenic races of Gibberella zeae is postulated.

scholarly journals Genome-Wide Characterization of Jasmonates Signaling Components Reveals the Essential Role of ZmCOI1a-ZmJAZ15 Action Module in Regulating Maize Immunity to Gibberella Stalk Rot

2021 ◽  
Vol 22 (2) ◽  
pp. 870
Author(s):  
Liang Ma ◽  
Yali Sun ◽  
Xinsen Ruan ◽  
Pei-Cheng Huang ◽  
Shi Wang ◽  
...  
Keyword(s):  
Fusarium Graminearum ◽  
Maize Production ◽  
Stalk Rot ◽  
Enhanced Resistance ◽  
Genome Wide ◽  
Susceptibility Factors ◽  
A Genome ◽  
Function Module ◽  
Ja Signaling ◽  
Signaling Components

Gibberella stalk rot (GSR) by Fusarium graminearum causes significant losses of maize production worldwide. Jasmonates (JAs) have been broadly known in regulating defense against pathogens through the homeostasis of active JAs and COI-JAZ-MYC function module. However, the functions of different molecular species of JAs and COI-JAZ-MYC module in maize interactions with Fusarium graminearum and regulation of diverse metabolites remain unknown. In this study, we found that exogenous application of MeJA strongly enhanced resistance to GSR. RNA-seq analysis showed that MeJA activated multiple genes in JA pathways, which prompted us to perform a genome-wide screening of key JA signaling components in maize. Yeast Two-Hybrid, Split-Luciferase, and Pull-down assays revealed that the JA functional and structural mimic coronatine (COR) functions as an essential ligand to trigger the interaction between ZmCOIa and ZmJAZ15. By deploying CRISPR-cas9 knockout and Mutator insertional mutants, we demonstrated that coi1a mutant is more resistant, whereas jaz15 mutant is more susceptible to GSR. Moreover, JA-deficient opr7-5opr8-2 mutant displayed enhanced resistance to GSR compared to wild type. Together, these results provide strong evidence that ZmJAZ15 plays a pivotal role, whereas ZmCOIa and endogenous JA itself might function as susceptibility factors, in maize immunity to GSR.

Detection of fungal decay by high-energy multiple impact (HEMI) testing

Holzforschung ◽  
2006 ◽  
Vol 60 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Christian Brischke ◽  
Christian Robert Welzbacher ◽  
Andreas Otto Rapp
Keyword(s):  
Structural Changes ◽  
Brown Rot ◽  
High Energy ◽  
White Rot ◽  
Fungal Decay ◽  
Highly Correlated ◽  
Thermally Modified Wood ◽  
Impact Milling ◽  
Multiple Impact ◽  
Steel Balls

Abstract The suitability of a previously described high-energy multiple impact (HEMI) test for the detection of early fungal decay was examined. The HEMI test characterises the treatment severity of thermally modified wood by stressing the treated material by thousands of impacts of pounding steel balls. This method differentiates between heat treatment intensities, which are manifest as structural changes in the wood. Similar changes in wood structure are known for wood decayed by fungi. Pine (Pinus sylvestris L.) decayed by brown rot and beech (Fagus sylvatica L.) decayed by white rot were tested. Mass loss caused by fungal decay and resistance to impact milling (RIM) determined in HEMI tests were found to be highly correlated. Testing of non-degraded pine, beech, and ash (Fraxinus exelsior L.) showed only marginal effects of wood density on RIM. Furthermore, annual ring angles and RIM of spruce (Picea abies Karst.) were not correlated. Accordingly, the detection of RIM reduction in decayed wood is not masked by variations in density and orientation of the annual rings. Previous results showed no adverse effects of weathering on RIM. Thus, the detection of fungal decay with HEMI tests is feasible not only for laboratory purposes, but also for wood in outdoor applications that has already undergone weathering.

scholarly journals Técnicas de aplicação de fungicida em trigo para o controle de Giberela (Gibberella zeae)

2003 ◽  
Vol 33 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Edivan Panisson ◽  
Walter Boller ◽  
Erlei Melo Reis ◽  
Laércio Hoffmann
Keyword(s):  
Fusarium Graminearum ◽  
Gibberella Zeae

Durante muitos anos, a giberela, causada pelo fungo Gibberella zeae (anamorfo Fusarium graminearum), foi considerada de importância secundária no sul do Brasil. O aumento da intensidade e da freqüência de ocorrência, tornou a giberela uma das doenças de maior importância na cultura do trigo. A giberela é uma doença de infecção floral e mesmo os fungicidas sistêmicos recomendados apresentam apenas efeito protetor das anteras. Os objetivos do presente trabalho foram avaliar a eficiência de controle e os efeitos nos grãos colhidos, de dois tipos de pontas de pulverização (leque e duplo leque), diferentes arranjos dos bicos na barra de aplicação e dois volumes de calda. A desuniformidade da antese aparece como um dos principais fatores envolvidos com a baixa eficiência dos fungicidas. Nos experimentos, realizados no ano 2000, a aplicação dos tratamentos reduziu significativamente a incidência, o número de espiguetas gibereladas e a severidade da doença, aumentando o rendimento de grãos. As pontas de pulverização, seus arranjos na barra e os volumes de calda utilizados comportaram-se de maneira semelhante em todas as variáveis avaliadas. O incremento no rendimento de grãos, obtido em relação à testemunha, sugere que se deve recomendar a aplicação de fungicidas para o controle da giberela, utilizando pontas que geram gotas finas a médias com volume de calda de 200 L.ha-1.

scholarly journals EVALUATION OF A BIO-ASSAY OF ETHER EXTRACTS FROM CORN (ZEA MAYS L.) AS A MEANS OF SCREENING INBREDS FOR RESISTANCE TO ROOT AND STALK ROT

1962 ◽  
Vol 42 (2) ◽  
pp. 302-307 ◽  
Author(s):  
N. J. Whitney ◽  
C. G. Mortimore
Keyword(s):  
Zea Mays ◽  
Fusarium Moniliforme ◽  
Zea Mays L ◽  
Gibberella Zeae ◽  
Assay Method ◽  
Stalk Rot ◽  
Fungus Growth ◽  
Bio Assay

In bio-assay tests ether extracts of the roots and of the stalks of both resistant and susceptible corn inbreds inhibited the growth of Fusarium moniliforme Sheld. and Gibberella zeae (Schw.) Petch, the level of inhibition varying over the season. However, the extracts of the resistant and susceptible inbreds did not differ consistently in their ability to inhibit fungus growth on any given date. Thus, this bio-assay method cannot be used to evaluate inbreds for resistance to root and stalk rot.

Corn Plant Maturity. IV. Effects on Digestibility of Corn Silage in Sheep

1968 ◽  
Vol 27 (2) ◽  
pp. 535 ◽  
Author(s):  
Ronald R. Johnson ◽  
K. E. McClure
Keyword(s):  
Corn Silage ◽  
Corn Plant ◽  
Plant Maturity

Physiological and Environmental Aspects of Ascospore Discharge in Gibberella zeae (Anamorph Fusarium graminearum)

Mycologia ◽  
2002 ◽  
Vol 94 (2) ◽  
pp. 181 ◽  
Author(s):  
Frances Trail ◽  
Haixin Xu ◽  
Rachel Loranger ◽  
David Gadoury
Keyword(s):  
Fusarium Graminearum ◽  
Gibberella Zeae ◽  
Environmental Aspects ◽  
Ascospore Discharge

scholarly journals Fungal Pathogens Affecting the Production and Quality of Medical Cannabis in Israel

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 882
Author(s):  
Shachar Jerushalmi ◽  
Marcel Maymon ◽  
Aviv Dombrovsky ◽  
Stanley Freeman
Keyword(s):  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Pathogenic Fungi ◽  
Plant Diseases ◽  
Soilborne Pathogens ◽  
Medical Cannabis ◽  
Plant Parts ◽  
Commercial Cultivation

The use of and research on medical cannabis (MC) is becoming more common, yet there are still many challenges regarding plant diseases of this crop. For example, there is a lack of formal and professional knowledge regarding fungi that infect MC plants, and practical and effective methods for managing the casual agents of disease are limited. The purpose of this study was to identify foliar, stem, and soilborne pathogens affecting MC under commercial cultivation in Israel. The predominant major foliage pathogens were identified as Alternaria alternata and Botrytis cinerea, while the common stem and soilborne pathogens were identified as Fusarium oxysporum and F. solani. Other important fungi that were isolated from foliage were those producing various mycotoxins that can directly harm patients, such as Aspergillus spp. and Penicillium spp. The sampling and characterization of potential pathogenic fungi were conducted from infected MC plant parts that exhibited various disease symptoms. Koch postulates were conducted by inoculating healthy MC tissues and intact plants with fungi isolated from infected commercially cultivated symptomatic plants. In this study, we report on the major and most common plant pathogens of MC found in Israel, and determine the seasonal outbreak of each fungus.

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