First Report of Rust Disease Caused by Puccinia liliacearum on Ornithogalum umbellatum From Indiana and Maryland With Notes on the Spread of the Rust Fungus in the United States

Soybean rust (SBR), caused by the obligate fungus Phakopsora pachyrhizi Syd. & P. Syd., was initially reported on soybean (Glycine max L.) in Louisiana in 2004 and has since been reported on soybean and/or kudzu (Pueraria lobata (Willd.) Ohwi) in 9 states in 2005, 15 states in 2006, and 19 states in 2007 (1). The host range of P. pachyrhizi includes plants that are all in the Fabaceae or legume family. Six plant species in the United States have been reported as hosts of P. pachyrhizi: soybean, kudzu, Florida beggarweed (Desmodium tortuosum (Sw) DC.), dry bean (Phaseolus vulgaris L.), lima bean (P. lunatus L.), and scarlet runner bean (P. coccineus L.) (4). On 17 April 2008, a rust disease was observed on a weedy legume host with red showy flowers that was growing with kudzu in an overgrown vacant lot in the understory of live oak trees (Quercus virginiana Mill.) in Citra, FL. The discovery was made during routine scouting of this Integrated Pest Management Pest Information Platform for Extension and Education (IPM PIPE) mobile sentinel plot (3). The plant was confirmed by University of Florida botanists to be Erythrina herbaceae L., commonly known as coral bean. Coral bean is native to the southeastern United States and also is planted as a perennial ornamental. A sample of leaves exhibiting rust pustules characteristic of P. pachyrhizi uredinia was collected and examined with a microscope. Brown-to-brick red, angular lesions that were 3 to 11 mm in diameter (average 6.75 mm) were observed on the undersides of the leaves of two trifoliates. Within these lesions, there were several uredinia, some exuding hyaline, echinulate urediniospores (20 × 25 μm). The visual diagnosis and the species of the rust fungus were confirmed to be P. pachyrizi by a real-time PCR protocol (2). The diagnosis on this new host was verified by a USDA, APHIS National Mycologist in Beltsville, MD. Coral bean may serve as an additional overwintering host for P. pachyrhizi in the southeast. To our knowledge, this is the first report of soybean rust caused by P. pachyrhizi on E. herbaceae. References: (1) R. S. C. Christiano and H. Scherm, Phytopathology 97:1428, 2007. (2) R. D. Frederick et al. Phytopathology 92:217, 2002. (3) S. A. Isard et al. Online publication. doi:10.1094/PHP-2006-0915-01-RV. Plant Health Progress, 2006. (4) T. L. Slaminko et al. Plant Dis. 92:767, 2008.

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First Report of Puccinia coronata var. coronata sensu stricto Infecting Alder Buckthorn in the United States

Plant Health Progress ◽

10.1094/php-01-17-0003-br ◽

2017 ◽

Vol 18 (2) ◽

pp. 84-86

Author(s):

Shawn C. Kenaley ◽

Geoffrey Ecker ◽

Gary C. Bergstrom

Keyword(s):

United States ◽

North America ◽

Host Range ◽

Geographic Distribution ◽

Phylogenetic Analyses ◽

Rust Fungus ◽

The United States ◽

Sensu Stricto ◽

Puccinia Coronata ◽

First Report

Field symptoms, host distribution, pathogen morphology, and phylogenetic analyses clearly demonstrated that the rust fungus infecting alder buckthorn in Connecticut is Puccinia coronata var. coronata sensu stricto. To our knowledge, this is the first report and confirmation of P. coronata var. coronata s.s. in the United States. Additional collections from purported aecial and telial hosts of P. coronata var. coronata s.s. are necessary to determine its host range, geographic distribution, and incidence within the United States and elsewhere in North America.

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First Report of Rust Caused by Puccinia allii on Wild Garlic in California

Plant Disease ◽

10.1094/pdis.2001.85.12.1290d ◽

2001 ◽

Vol 85 (12) ◽

pp. 1290-1290 ◽

Cited By ~ 2

Author(s):

S. T. Koike ◽

R. F. Smith

Keyword(s):

United States ◽

Allium Sativum ◽

Rust Fungus ◽

Morphological Characteristics ◽

Western United States ◽

Rust Disease ◽

Perennial Weed ◽

First Report ◽

Wild Garlic ◽

Puccinia Allii

Wild garlic (Allium vineale) is a bulbous perennial weed that was introduced from Europe and is now established throughout the eastern and much of the western United States. In 2001, wild garlic plants growing in Monterey County, CA were infected with a rust fungus. Uredinia and telia were present on leaves, resulting in small (2 to 5 mm long) lesions; however, leaf dieback and other symptoms were not observed. The orange urediniospores were spherical to ellipsoidal, echinulate, and mea-sured 26 to 30 × 25 to 28 μm. Telia were black in mass and divided into locules by fused paraphyses. Teliospores were located within the locules and were brown, smooth-walled, two-celled, and measured 40 to 50 × 17 to 20 μm. Teliospore pedicels were hyaline, usually fractured, and measured 3 to 15 μm. One-celled mesospores were not observed. Based on these morphological characteristics, the pathogen was identified as Puccinia allii (1,2). To our knowledge, this is the first report of rust caused by P. allii on wild garlic in California. Because of this finding, an experiment was designed to determine whether wild garlic could be a source of rust inoculum for commercial allium crops. Wild garlic bulbs were planted in 4-in. pots (10 cm square) and grown in a greenhouse. At the 4- to 5-leaf stage the plants were transplanted in a garlic (Allium sativum) cultivar trial that had been inoculated with an isolate of P. allii from garlic. When plants were evaluated 2 months later, uredinia and telia were observed on the wild garlic plants, and the fungus was confirmed to be P. allii. Wild garlic, therefore, could be a source of overwintered inocula for the rust disease that occurs on commercial garlic and onion (A. cepa) in California (2). References: (1) D. M. Jennings et al. Mycol. Res. 94:83, 1990. (2) S. T. Koike et al. Plant Dis. 85:585, 2001.

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First Report of Puccinia lagenophorae on Common Groundsel (Senecio vulgaris) in Canada

Plant Disease ◽

10.1094/pdis-91-8-1058c ◽

2007 ◽

Vol 91 (8) ◽

pp. 1058-1058 ◽

Cited By ~ 5

Author(s):

W. L. Bruckart ◽

A. S. McClay ◽

S. Hambleton ◽

R. Tropiano ◽

G. Hill-Rackette

Keyword(s):

United States ◽

Its Region ◽

The United States ◽

Representative Specimen ◽

Rust Disease ◽

First Report ◽

Senecio Vulgaris ◽

Pcr Products ◽

Plant Pathol ◽

Common Groundsel

Rust disease on common groundsel was independently collected from two backyard gardens in Alberta, Canada during 2005, the first on September 11 in Sherwood Park (53.542°N, 113.262°W) and the second on September 18 in Edmonton (53.463°N, 113.593°W). Leaves of each specimen had clusters of orange, cup-shaped aecia, bordered by recurved peridia, the principal macroscopic signs of disease. Infected plants had twisted stems and deformed leaves. Spores of isolates from the two locations were (mean diameter [± s.d.; range]) 14.6 (± 1.4; 11.4 to 18.9) × 12.5 (± 1.1; 9.1 to 16.2) μm, orange, oval or angular, and many had refractive granules (3). Genomic DNA was extracted from small leaf pieces with multiple aecia, and the complete internal transcribed spacer (ITS) region of the rust was sequenced from PCR products. The sequences determined for a representative specimen from each location were identical, including two areas of ambiguity in the ITS1 spacer region. At position 7 were two overlapping peaks (A and C), and near position 130, sequencing failed because of a suspected insertion/deletion in some ITS copies. Difficulties of sequencing through this cytosine-rich area were reported by Littlefield et al. (3). Data from cloned PCR products confirmed the presence of two ITS genotypes in each DNA extract, one identical to a sequence published for Puccinia lagenophorae on Senecio vulgaris from the United Kingdom (GenBank Accession No. AY808060 (2), and the other identical to a sequence from the United States (GenBank Accession No. AY852264) (3). They differ by an A/C transversion at position 7 and an indel, an 8/9 base poly-C run beginning at position 130. Telia and teliospores were not observed in any of the 2005 samples (some collected as late as November) or in the 2006 Edmonton site samples. Identification of the pathogen as P. lagenophorae was based on host plant symptoms (3) and molecular characters. The original source of inoculum for these infections is unknown, but on December 5, 2006, diseased specimens with sporulating aecia were found beneath 45 cm of snow at the Edmonton location, in a garden area that had not been weeded during the summer. There is reported evidence that teliospores are not functional and that P. lagenophorae overwinters on infected plants that develop aecia in the spring (1). Specimens have been deposited at the Arthur Herbarium, Purdue University, West Lafayette, IN (Vouchers PUR N5414–N5417) and the National Mycological Herbarium of Canada, Ottawa, ON (Vouchers DAOM 237844, 237845, 237961, 237962, 237982, and 237990). The two cloned variants of the ITS sequence were deposited in GenBank (Accession Nos. EF212446 and EF212447). To our knowledge, this is the first report of groundsel rust caused by P. lagenophorae in Canada (G. Barron, personal communication, has images from Guelph in 2004 but no specimens were examined or preserved). Groundsel rust has been found at several locations in the United States (3) and has been reported on more than 60 species in several genera (4). Questions remain about the amount of damage that P. lagenophorae will cause to groundsel in North America and whether it will affect native Senecio species and their relatives. References: (1) J. Frantzen and H. Müller-Schärer. Plant Pathol. 48:483, 1999. (2) B. Henricot and G. Denton. Plant Pathol. 54:242, 2005. (3) L. Littlefield et al. Ann. Appl. Biol. 147:35, 2005. (4) M. Scholler. J. Plant Dis. Prot. 105:239, 1998.

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First Occurrence of Puccinia lagenophorae Causing Rust Disease on Common Groundsel in North America

Plant Disease ◽

10.1094/pdis.2001.85.3.335c ◽

2001 ◽

Vol 85 (3) ◽

pp. 335-335 ◽

Cited By ~ 3

Author(s):

M. Scholler ◽

Arthur Herbaria ◽

Kriebel Herbaria ◽

S. T. Koike

Keyword(s):

United States ◽

North America ◽

South America ◽

Rust Fungus ◽

First Record ◽

The United States ◽

Early Summer ◽

Rust Disease ◽

Worldwide Distribution ◽

Common Groundsel

Common groundsel (Senecio vulgaris, Asteraceae) is native to Europe and is now a common weed mainly in disturbed habitats of almost worldwide distribution. In November 2000, groundsel plants growing adjacent to lettuce fields in California's coastal Salinas Valley (Monterey County) showed symptoms of rust. In a 0.2-ha survey area, 75% of the plants were infected. Examination of weeds growing in four residential blocks also uncovered infected groundsel. Densely clustered, orange aecia were observed on leaves and stems. Stems were swollen where aecia had formed. Blossom and fruit formation was not notably reduced, although some involucral bracts were infected. Aeciospores measured 14 to 18 μm × 12.5 to 15 μm (fresh material). Telia were not found. The pathogen was identified as Puccinia lagenophorae Cooke, a rust fungus that is native to Australia and New Zealand and infects plants of the subfamily Asteroideae (family Asteraceae) (3). P. lagenophorae is an autoecious species forming only repeating aecia (stage I) and telia (stage III). There are six other rusts of Senecio that occur in the United States (1) that readily form aecia but not telia on Senecio spp. When only aecia are observed on Senecio, which is typical for P. lagenophorae (2), the following features can be used to differentiate it from these other species: no pycnia (stage 0) are formed; aecia are formed repeatedly; systemic growth that results in deformation of the host, including formation of galls with dense clusters of aecia on the stem; poorly developed aecial peridium; and aeciospores small, measuring 12.5 to 18.5 μm × 10.0 to 16.0 μm (4). In addition, P. lagenophorae forms aecia even at the end of the year in northern temperate zones, whereas heteroecious species form aecia only in spring and early summer (2). This is the first record of P. lagenophorae in North America. Specimens were deposited in the Arthur Herbarium, Purdue University. Outside its native habitat, this fungus has been found in Europe, Africa, the Middle East, and South America. There are about 60 known host species of P. lagenophorae (3) including ornamentals such as Bellis perennis, Calendula officinalis, and Senecio cruentus. The pathogen may have been introduced to North America via land from South America through Central America, or by the importation of ornamentals that were either infected with rust or infested with diseased groundsel. References: (1) D. F. Farr et al. 1989. Fungi on Plants and Plant Products in the United States. American Phytopathological Society, St. Paul, MN. (2) M. Scholler. Regensb. Myk. Schr. 6:1, 1996. (3) M. Scholler. J. Plant Dis. Prot. 105:239, 1998. (4) I. Wilson et al. Trans. Brit. Mycol. Soc. 48:501, 1965.

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First Report of Plumeria spp. Rust Caused by Coleosporium plumeriae in Louisiana and Malaysia and Catheranthus roseus, a New Host of this Rust

Plant Disease ◽

10.1094/pdis-94-2-0272c ◽

2010 ◽

Vol 94 (2) ◽

pp. 272-272 ◽

Cited By ~ 6

Author(s):

G. E. Holcomb ◽

M. C. Aime

Keyword(s):

United States ◽

Baton Rouge ◽

Pacific Islands ◽

The United States ◽

Plant Diseases ◽

Nerium Oleander ◽

Urban Landscapes ◽

Rust Disease ◽

Madagascar Periwinkle ◽

First Report

Plumeria spp., native to tropical America, are popular small trees grown widely in tropical areas of the world and as potted plants elsewhere. P. rubra and P. obtusa cultivars and hybrids are most common. A rust disease of a Plumeria sp. (likely P. rubra based on pointed leaf tips, leaves more than 18 cm (7 inches) long, and high rust susceptibility) was observed in November 2008 and again in June 2009 on homeowner plants in Baton Rouge, LA. A survey of five Baton Rouge retail nurseries in September 2009 revealed that 87% (90 of 103) of the plumeria plants were heavily infected with rust. Early symptoms included numerous 1-mm chlorotic spots on adaxial leaf surfaces followed by leaf chlorosis, necrosis, and abscission. Uredinia were numerous, mostly hypophyllous and yellowish orange. Urediniospores were catenulate, orange en masse, verrucose, globose, ovoid, ellipsoidal or angular, and measured 21.8 to 41.9 × 16.4 to 32.8 μm (average 29.4 × 22.6 μm). The rust was identified as Coleosporium plumeriae Pat. (= C. plumierae) (3). Teliospores were not found during this study. Pathogenicity tests were performed by spraying urediniospores (20,000/ml of deionized water) on three healthy Thai hybrid plumeria plants. Five leaves of each plant were misted with water and covered with plastic bags and three to five leaves were inoculated. Plants were held at 27°C for 27 h in a dew chamber and then moved outdoors. Typical rust symptoms and uredinia with urediniospores developed in 10 days on all inoculated leaves while noninoculated leaves remained healthy. Characteristics and spore measurements matched those of the rust from original infected plants. Additional plumeria rust inoculations were made to other Apocynaceae family members that included Allamanda cathartica, Catheranthus roseus (Madagascar periwinkle), Mandevilla splendens, Nerium oleander, and Vinca major. Catheranthus roseus was very susceptible to C. plumeriae with chlorotic leaf spots developing on the six inoculated plants after 8 days and uredinia with urediniospores appearing after 11 days. None of the other plant genera were susceptible to the rust. Plumeria rust was also observed on plumeria trees in urban landscapes in peninsular (Penang) and Bornean (Kota Kinabalu, Sabah) Malaysia in December 2007. To confirm identity, ~1,000 bp of nuclear rDNA 28S subunit from each (Lousiana, Penang, and Kota Kinabalu) was sequenced with rust-specific primers (1) and shared 100% identity (GenBank No. GU145555-6). Plumeria rust was first found on the island of Guadeloupe (3) and then spread to Central and South America. It has been known from Florida since 1960 under the synonym C. domingense (2), but has not been reported elsewhere in the continental United States. In more recent years, plumeria rust has spread to Hawaii, many Pacific islands, India, China, Taiwan, Thailand, Australia, and Nigeria (4). To our knowledge, this is the first report of plumeria rust from Louisiana and Malaysia and of susceptibility of another member of the Apocynaceae, Madagascar periwinkle, to C. plumeriae. Voucher material from Louisiana and Malaysia has been deposited in the Mycology Herbarium of Louisiana State University (LSUM). References: (1) M. C. Aime. Mycoscience 47:112, 2006. (2) Anonymous. Index of Plant Diseases in the United States. U.S. Dept. Agric. Handb. No. 165. Washington, D.C., 1960. (3) N. Patouillard. Bull. Soc. Mycol. Fr. 18:171, 1902. (4) C. To-Anun et al. Nat. Hist. J. Chulalongkorn Univ. 4:41, 2004.

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First Report of Puccinia jaceae var. solstitialis Pycnia on Yellow Starthistle in the United States

Plant Disease ◽

10.1094/pd-90-1362a ◽

2006 ◽

Vol 90 (10) ◽

pp. 1362-1362 ◽

Cited By ~ 6

Author(s):

A. J. Fisher ◽

W. L. Bruckart ◽

M. B. McMahon ◽

D. G. Luster ◽

L. Smith

Keyword(s):

United States ◽

Life Cycle ◽

Polymerase Chain Reaction Amplification ◽

Rust Fungus ◽

Field Isolate ◽

Pcr Primers ◽

The United States ◽

Its2 Region ◽

Yellow Starthistle ◽

First Report

The rust fungus Puccinia jaceae (Otth) var. solstitialis field isolate FDWSRU 84-71 (formerly TR 84–96) was first released in California for the biological control of yellow starthistle (YST; Centaurea solstitialis) in July 2003. This isolate was collected by S. S. Rosenthal in 1984, east of Yarhisar and Hafik (SIVAS), Turkey. It is macrocyclic and autoecious (1), completing its entire life cycle on YST. Observations were made in field plots west of Woodland, CA that had been inoculated on a monthly regimen between January and June of 2005. On February 22 and March 2, 2006, pycnia were observed in and around one of the plots (38°42.767′N, 121°53.732′W at an altitude of 57 m). Pycnia were yellow, flask shaped, and small (less than 100 μm in diameter), occurring in clusters on abaxial leaf surfaces or on petioles. DNA was isolated from pycnial and uredinial samples collected from the site and used for polymerase chain reaction amplification of the ITS2 region with P. jaceae specific primers. Sequences of amplicons from both samples were identical to FDWSRU 84-71 (GenBank Accession No. AF047728). Further amplification of pycnial DNA with PCR primers specific to the released P. jaceae isolate (2) produced the expected 851-bp amplicon. To our knowledge, this is the first report of pycnia from Puccinia jaceae var. solstitialis in the United States, suggesting that the YST rust is fully functional and completes its life cycle in California. References: (1) D. B. O. Savile. Can. J. Bot. 48:1553, 1970. (2) L. F. Yourman and D. G. Luster. Biol. Control 29:73, 2004.

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First Report of Asian Soybean Rust Caused by Phakopsora pachyrhizi on Soybean in Alabama

Plant Disease ◽

10.1094/pd-90-0112c ◽

2006 ◽

Vol 90 (1) ◽

pp. 112-112 ◽

Cited By ~ 5

Author(s):

J. M. Mullen ◽

E. J. Sikora ◽

J. M. McKemy ◽

M. E. Palm ◽

L. Levy ◽

...

Keyword(s):

United States ◽

The United States ◽

Soybean Rust ◽

Phakopsora Pachyrhizi ◽

Rust Disease ◽

First Report ◽

Leaf Spots ◽

Asian Soybean Rust ◽

Leaf Surfaces ◽

Soybean Leaves

On November 4, 2004, soybean leaves (Glycine max (L.) Merr) were submitted to the Auburn University Plant Diagnostic Lab by a State Department of Agriculture and Industries Inspector. Samples were collected from an 80-ha field of soybean plants in a late-reproductive-growth stage in Mobile County, Alabama. Under microscopic examination, leaves showed rust pustules in advanced stages of development with urediniospores and sori characteristic of Phakopsora spp. Uredinia were ostiolate in small, brown, angular leaf spots (2 to 3 mm) on lower leaf surfaces. Urediniospores were pale yellow-to-white, globose or ovate, 20 to 40 × 15 to 25 μm. In a subsequent visit to the field, symptoms and signs of the rust disease were observed on plants bordering the edge of the field since the majority of plants were senescent. Tan lesions on lower leaf surfaces contained small pustules surrounded by a small zone of slightly discolored necrotic tissue. Masses of tan spores covered the lower leaf surface pustules. Leaves were mailed overnight to the USDA National Identification Services (Mycology) Laboratory in Beltsville, MD. The fungal structures were confirmed to be a Phakopsora sp., and the sample was forwarded to the USDA National Plant Germplasm and Biotechnology Laboratory in Beltsville, MD. DNA was extracted from leaf pieces containing sori using the Qiagen DNeasy Plant Mini kit (Qiagen, Valencia, CA). Phakopsora pachyrhizi was detected using a real-time polymerase chain reaction (PCR) protocol (1) performed in a Cepheid SmartCycler (Sunnyvale, CA). The PCR master mix was modified to include OmniMix beads (Cepheid). The field and microscopic suspect diagnosis of P. pachyrhizi was confirmed officially by APHIS on November 18, 2004. This was the fourth USDA official confirmation of Asian soybean rust in the continental United States during 2004, and to our knowledge, this is the first report of the disease in Alabama. This report helps confirm that early occurrences of Asian soybean rust in the United States were present in other areas in addition to the first reported finding in Louisiana (2). References: (1) R. D. Frederick et al. Phytopathology 92:217, 2002. (2) R. W. Schneider et al. Plant Dis. 89:774, 2005.

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First Report of Pathogenicity of Fusarium sporotrichioides and Fusarium acuminatum on Sunflowers in the United States

Plant Health Progress ◽

10.1094/php-2010-0915-02-br ◽

2010 ◽

Vol 11 (1) ◽

pp. 42 ◽

Cited By ~ 4

Author(s):

F. Mathew ◽

B. Kirkeide ◽

T. Gulya ◽

S. Markell

Keyword(s):

United States ◽

Helianthus Annuus ◽

The United States ◽

Fusarium Sporotrichioides ◽

Charcoal Rot ◽

Koch’S Postulates ◽

First Report ◽

Fusarium Acuminatum ◽

Helianthus Annuus L ◽

Koch's Postulates

Widespread infection of charcoal rot was observed in a commercial sunflower field in Minnesota in September 2009. Based on morphology, isolates were identified as F. sporotrichioides and F. acuminatum. Koch's postulates demonstrated pathogencity of both species. To our knowledge, this is the first report of F. sporotrichoides and F. acuminatum causing disease on Helianthus annuus L. in the United States. Accepted for publication 23 August 2010. Published 15 September 2010.

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First Report of Wisteria vein mosaic virus in Wisteria sinensis in the United States of America

Plant Health Progress ◽

10.1094/php-2008-0818-01-br ◽

2008 ◽

Vol 9 (1) ◽

pp. 42 ◽

Cited By ~ 1

Author(s):

Rayapati A. Naidu ◽

Gandhi Karthikeyan

Keyword(s):

United States ◽

Mosaic Virus ◽

United States Of America ◽

The United States ◽

Home Gardens ◽

First Report ◽

Woody Perennial ◽

Wisteria Sinensis ◽

First Time ◽

Wisteria Vein Mosaic Virus

The ornamental Chinese wisteria (Wisteria sinensis) is a woody perennial grown for its flowering habit in home gardens and landscape settings. In this brief, the occurrence of Wisteria vein mosaic virus (WVMV) was reported for the first time in Chinese wisteria in the United States of America. Accepted for publication 18 June 2008. Published 18 August 2008.

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