scholarly journals First Report of White Rust of Rocket (Eruca sativa) Caused by Albugo candida in Montenegro

Plant Disease ◽  
2019 ◽  
Vol 103 (1) ◽  
pp. 163-163
Author(s):  
J. Latinović ◽  
N. Latinović ◽  
J. Jakše ◽  
S. Radišek
Keyword(s):  
Eruca Sativa ◽  
First Report ◽  
Albugo Candida ◽  
White Rust

scholarly journals First Report of White Rust of Rocket (Eruca sativa) Caused by Albugo candida in South Africa

Plant Disease ◽  
2015 ◽  
Vol 99 (2) ◽  
pp. 290-290 ◽  
Author(s):  
E. Mangwende ◽  
J. B. Kalonji Kabengele ◽  
M. Truter ◽  
T. A. S. Aveling
Keyword(s):  
South Africa ◽  
Agricultural Research ◽  
Plant Protection ◽  
Control Treatment ◽  
Eruca Sativa ◽  
Voucher Specimen ◽  
First Report ◽  
Albugo Candida ◽  
White Rust ◽  
Leaf Surfaces

Garden rocket (Eruca sativa syn.: E. vesicaria subsp. sativa (Mill) Thell.) is an annual plant of the Brassicaceae grown for fresh consumption as a salad vegetable. During winter (May to July) of 2013 and 2014 in South Africa, typical symptoms of white rust were observed in two commercial crops (each ~0.5 ha) of the garden rocket cv. Rucola coltivata in Centurion, Gauteng Province, at 33 and 80% incidence, respectively. Symptomatic leaves were deposited in the National Collection of Fungi, Plant Protection Research Institute, Agricultural Research Council, Pretoria, South Africa (PREM 61073). Early infections appeared as white to cream, blister-like sori on the lower leaf surfaces, and pale yellow lesions on the corresponding upper leaf surfaces. Later stages of infection were characterized by coalescing of lesions into large, irregular, necrotic blotches and development of additional sori on the petioles and stems. Sporangiophores were hyaline, clavate or cylindrical, and measured 24 to 30 × 11 to 14 μm (n = 50). Sporangia developed in basipetal chains and were hyaline, globose or polyangular, and 15 to 20 μm (n = 100). Based on these morphological characters and the host plant, the pathogen was identified as Albugo candida (Pers.) Kunze (2). Genomic DNA was extracted using the DNeasy Plant Mini DNA extraction kit (Qiagen) from sori containing sporangia collected from naturally infected leaves, according to the manufacturer's specifications. The internal transcribed spacer (ITS) region of ribosomonal DNA (rDNA) and the cytochrome c oxidase subunit II (COX2) region were amplified and sequenced (1). The ITS (GenBank Accession No. KM588081) and COX2 (KM588082) sequences confirmed identity of the pathogen as A. candida with 100% homology to the corresponding sequences of several A. candida isolates, including DQ418503 for the ITS sequence and DQ418514 for the COX2 sequence, of a voucher specimen of A. candida on E. sativa (BPI 184870) from Pakistan. Inoculum was prepared by scraping sporangia from infected leaves of the cv. Rucola coltivata collected from the 2014 field and placing the material in sterilized, distilled water (SDW) for 12 h at 5°C to induce zoospore formation. Pathogenicity tests were performed by spraying a suspension of 1 × 105 sporangia/ml onto each of 10 5-week-old rocket seedlings of the cv. Rucola coltivata. Ten additional seedlings were inoculated similarly with SDW to serve as a control treatment. The plants were maintained at 12 to 15°C and 95% RH for 72 h (3) before being moved to a shaded greenhouse at 20 to 24°C and 90% RH. Control plants remained symptomless, whereas white rust symptoms similar to those observed in the original fields developed on leaves of inoculated seedlings 10 to 14 days later, demonstrating that A. candida was the causal agent of the disease on E. sativa. To our knowledge, this is the first report of A. candida infecting garden rocket in South Africa. References: (1) Y.-J. Choi et al. Mol. Phylogenet. Evol. 40:400, 2006. (2) K. Mukerji. Descriptions of Pathogenic Fungi and Bacteria No. 458. CMI, Kew, Surrey, UK, 1975. (3) M. J. Sullivan et al. Plant Dis. 86:753, 2002.

scholarly journals First Report of Albugo candida Causing White Rust on Lunaria annua in Italy

Plant Disease ◽  
2011 ◽  
Vol 95 (1) ◽  
pp. 72-72
Author(s):  
I. Camele ◽  
S. M. Mang ◽  
G. L. Rana
Keyword(s):  
Direct Sequencing ◽  
Morphological Characteristics ◽  
Its Region ◽  
First Report ◽  
Albugo Candida ◽  
White Rust ◽  
Landscape Plant ◽  
Average Maximum ◽  
Mother Plants ◽  
Health Progress

Money plant or annual honesty (Lunaria annua L.) is an ornamental landscape plant used in flower beds and borders and also in flower arrangements. It is a biennial plant with large, pointed, oval leaves. Plants of L. annua showing white-to-cream, blister-like lesions on leaves and siliques (2) were found in private gardens where approximately 800 plants of 1,000 (approximately 80 to 90%) that were observed showed symptoms. The disease was also found in two ornamental nurseries, although it was limited to a few mother plants because of extensive fungicide treatments. The gardens and ornamental nurseries were located in Potenza Province (Basilicata Region, southern Italy). Sporangiophores were mostly straight or arched and almost cylindrical with attenuated base and flat or rounded apex and measured 29.2 to 33.4 × 12.8 to 13.4 μm. Sporangia, produced in chains and joined by short connectives, exhibited a spherical or angular shape, were subhyaline, contained vacuoles, and had average maximum and minimum diameters ranging from 15.8 to 18.8 and 14 to 16 μm, respectively. The morphological characteristics closely resembled those reported for Albugo candida (Pers.) Kuntze (3). Sori were collected from naturally and artificially inoculated tissues of L. annua, with the aid of a stereomicroscope, and used to extract genomic DNA via a DNeasy Plant Mini DNA extraction kit (Qiagen, Hilden, Germany) according to the manufacturer's directions. The extracted DNA was used as a template for amplification of the internal transcribed spacer (ITS) region of rDNA with primer pair ITS4/DC6 (1,4) and sequenced. One sequence, GenBank Accession No. GQ328846, matched several sequences of A. candida (Pers). Kuntze (e.g., GenBank Accession Nos. GQ328837, GQ328836, GQ328835, GQ328834, and AF271231), showing 98% identity. Pathogenicity tests were performed and repeated twice. Leaves of 10 healthy seedlings of L. annua were surface cleaned during several washings with distilled water and then spray inoculated with a suspension of 103 sporangia/ml of A. candida. Five healthy seedlings were spray inoculated with the same volume of sterile water and served as controls. Inoculated seedlings were maintained in a moist chamber for 48 h at 20°C before being moved to a shaded glasshouse at 16 to 24°C and 90% relative humidity. White rust symptoms, similar to those observed in natural conditions, appeared on leaves of inoculated seedlings 10 to 14 days later, demonstrating that A. candida was the causal agent of the disease. Control plants remained symptomless. White rust has been reported on L. annua in Europe (Czech Republic, Germany, Poland, and the United Kingdom) and in the northwestern United States (3). To our knowledge, this is the first report of A. candida infecting annual honesty plant in Italy. References: (1) P. Bonants et al. Eur. J. Plant Pathol. 103:345, 1997. (2) D. Choi et al. Mycotaxon 53:261, 1995. (3) D. A. Glawe et al. Online publication. doi:10.1094/PHP-2004-0317-01-HN. Plant Health Progress, 2004. (4) T. J. White et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols. A Guide to Methods and Applications. Academic Press, San Diego, CA, 1990.

scholarly journals First Occurrence of White Rust of Arugula, Caused by Albugo candida

Plant Disease ◽  
1999 ◽  
Vol 83 (9) ◽  
pp. 877-877 ◽  
Author(s):  
H. J. Scheck ◽  
S. T. Koike
Keyword(s):  
Brassica Campestris ◽  
Secondary Infection ◽  
Eruca Sativa ◽  
Santa Barbara ◽  
Albugo Candida ◽  
White Rust ◽  
First Occurrence ◽  
Pathogenicity Tests ◽  
Zoospore Formation ◽  
Plant Pathol

In California, arugula (Eruca sativa) is grown commercially as a component of specialty mixed green salads. In 1999, white rust was observed on arugula in the central coast county of Santa Barbara. Symptoms consisted of white, blisterlike sori beneath the raised host epidermis on the underside of leaves from the seedling stage onward. Secondary infection of flower heads led to staghead development. Affected plantings were severely diseased and were not harvested. The pathogen was confirmed to be Albugo candida (Pers.) Kunze (2). Sporangia diameters ranged from 19.5 to 22.2 μm. Inoculum for pathogenicity tests was prepared by scraping sporangia from infected leaves and incubating them in sterile, distilled water for 12 h at 5°C to induce zoospore formation (1). The following plants were then spray inoculated: arugula, cauliflower (Brassica oleracea subsp. botrytis), Chinese cabbage (Brassica campestris subsp. pekinensis), Japanese mustard (Brassica campestris subsp. nipposinica), red mustard (Brassica juncea subsp. rugosa), and tah tsai (Brassica campestris subsp. narinosa). Twelve plants of each species were inoculated with 1.5 × 104 spores per ml at the six- to eight-leaf stage. Plants were incubated in a dew chamber for 48 h at 18°C and then maintained in a greenhouse at 20 to 24°C. After 10 to 12 days, white rust pustules similar to the original symptoms observed in the field developed only on arugula. The experiment was conducted three times and the results were the same. Distinct biological races of A. candida have been described for some Brassicaceae hosts of white rust (1). This is the first report of white rust of arugula in California caused by A. candida. References: (1) R. S. Pidskalny and S. R. Rimmer. Can. J. Plant Pathol. 7:283, 1985. (2) G. S. Pound and P. H. Williams. Phytopathology 53:1146, 1963.

First Report of White Rust of Lunaria annua Caused by Albugo candida in North America

2004 ◽  
Vol 5 (1) ◽  
pp. 23
Author(s):  
Dean A. Glawe ◽  
Jenny R. Glass ◽  
Melodie L. Putnam
Keyword(s):  
North America ◽  
First Report ◽  
Albugo Candida ◽  
White Rust ◽  
Landscape Plant

Lunaria annua, or money plant, is grown as an ornamental landscape plant and is used in dried flower arrangements. This report provides the first documentation of white rust (caused by the fungus Albugo candida) on L. annua in North America, and describes the symptoms and morphology of A. candida useful for identification. Accepted for publication 24 February 2004. Published 17 March 2004.

scholarly journals First Report of White Rust of Arugula Caused by Albugo candida in Argentina

Plant Disease ◽  
2005 ◽  
Vol 89 (2) ◽  
pp. 207-207 ◽  
Author(s):  
R. Zapata ◽  
A. M. Romero ◽  
P. H. Maseda
Keyword(s):  
Buenos Aires ◽  
Pathogenic Fungi ◽  
Buenos Aires Province ◽  
Eruca Sativa ◽  
Abaxial Epidermis ◽  
Albugo Candida ◽  
White Rust ◽  
Plastic Bags ◽  
Pathogenicity Tests ◽  
Zoospore Formation

Production of arugula (Eruca sativa) has increased greatly in Argentina. Since 2002, particularly during the fall, a foliar disease has affected commercial crops in Capilla del Señor (northeast of Buenos Aires Province, Argentina). The disease appeared in foci, spreading throughout the whole production field or greenhouse. Severely affected crops were plowed under. Diseased leaves were chlorotic and had white sori that emerged through the abaxial epidermis. Sori corresponded to the white rust agent, Albugo candida (Pers.) Kunze (1). Sporangiophores were hyaline and clavate, and sporangia were globose and hyaline with a mean diameter of 16.2 μm (14.2 to 19.2 μm). Pathogenicity tests were performed by spraying a suspension of 106 zoospores/ml or 5 × 104 sporangia/ml on four healthy 30-day-old arugula plants. Inoculum was prepared by scrapping sporangia from infected leaves. Sporangia were used directly or incubated in sterile distilled water (SDW) for 14 h at 5°C to induce zoospore formation (2). Four additional healthy plants were sprayed with SDW to serve as controls. Plants were kept in plastic bags for 48 h and maintained in the greenhouse thereafter. White rust symptoms, similar to those observed on the original plants from the field, were observed on inoculated plants 10 days after inoculation. To our knowledge, this is the fist report of A. candida on arugula in Argentina. References: (1) K. Mukerji. No. 458 in: Descriptions of Pathogenic Fungi and Bacteria. CMI, Kew, Surrey, UK, 1975. (2) H. Scheck and S. Koike. Plant Dis. 83:877, 1999.

scholarly journals First Report of Leaf Spot Caused by Colletotrichum kahawae on Cultivated Rocket (Eruca sativa) in Italy

Plant Disease ◽  
2016 ◽  
Vol 100 (6) ◽  
pp. 1240-1240 ◽  
Author(s):  
A. Garibaldi ◽  
G. Gilardi ◽  
I. Puglisi ◽  
S. O. Cacciola ◽  
M. L. Gullino
Keyword(s):  
Leaf Spot ◽  
Eruca Sativa ◽  
First Report ◽  
Colletotrichum Kahawae

Sporulation Capacity and Longevity of Puccinia horiana Teliospores in Infected Chrysanthemum Leaves

2013 ◽  
Vol 14 (1) ◽  
pp. 11 ◽  
Author(s):  
Morris R. Bonde ◽  
Cristi L. Palmer ◽  
Douglas G. Luster ◽  
Susan E. Nester ◽  
Jason M. Revell ◽  
...  
Keyword(s):  
United States ◽  
Fungal Pathogen ◽  
Local Environment ◽  
The United States ◽  
First Report ◽  
White Rust ◽  
Teliospore Germination ◽  
Sporulation Capacity ◽  
Leaf Necrosis ◽  
Puccinia Horiana

Puccinia horiana Henn., a quarantine-significant fungal pathogen and causal agent of chrysanthemum white rust (CWR), was first discovered in the United States in 1977 and later believed to have been eradicated. Recently, however, the disease has sporadically reappeared in the northeastern US. Possible explanations for the reappearance include survival of the pathogen in the local environment, and reintroduction from other locations. To determine the possibility that the pathogen might be overwintering in the field, we undertook the study described here. Results from the study showed that P. horiana teliospores, imbedded in infected leaves, were capable of sporulating 2 weeks after inoculation, and this capacity continued until the leaf became necrotic and desiccated. This is the first report of the extreme susceptibility of P. horiana teliospores to leaf necrosis and desiccation and suggests that field infections following winter are unlikely to originate from teliospores. Teliospore germination on excised leaves was shown to be inhibited by light. Accepted for publication 3 April 2013. Published 23 August 2013.

Expression and relationships of resistance to white rust (Albugo candida) at cotyledonary, seedling, and flowering stages in Brassica juncea germplasm from Australia, China, and India

2007 ◽  
Vol 58 (3) ◽  
pp. 259 ◽  
Author(s):  
C. X. Li ◽  
K. Sivasithamparam ◽  
G. Walton ◽  
P. Salisbury ◽  
W. Burton ◽  
...  
Keyword(s):  
Plant Growth ◽  
Brassica Juncea ◽  
Environmental Conditions ◽  
Host Resistance ◽  
Growth Stages ◽  
Sources Of Resistance ◽  
Albugo Candida ◽  
White Rust ◽  
China And India ◽  
Canola Quality

White rust (Albugo candida) is a highly destructive disease of oilseed Brassicas such as Brassica juncea and B. rapa. Most commercial B. juncea or B. rapa varieties are highly susceptible and yield losses from combined infection of leaves and inflorescences can be up to 20% or 60% in Australia and India, respectively. In Australia, canola-quality B. juncea has been developed to extend oilseed Brassica production into lower rainfall areas, with the first commercial B. juncea canola-quality variety planned for release in 2006. It is essential to identify useful sources of host resistance in B. juncea as breeding and/or selection of material for resistance is the most cost-effective method of delivering control for farmers. Three experiments were undertaken under controlled-environmental conditions to identify the best methods of characterising host resistance and to identify sources of resistance in B. juncea germplasm from Australia, China, and India. Forty-four B. juncea genotypes, viz. 22 from India, 12 from Australia, and 10 from China, were tested. Four Chinese genotypes (CBJ-001, CBJ-002, CBJ-003, CBJ-004) and one Australian genotype (JR049) consistently showed high resistance to A. candida across the different plant growth stages against a pathotype prevailing in Australia. Similarly, the most susceptible genotypes (viz. Indian genotypes RH781, RL1359, RH819) were extremely susceptible irrespective of the plant growth stage. Overall, although disease severity on cotyledons and leaves at the different growth stages was significantly and positively correlated, there was, however, no significant correlation between the number of stagheads and any of the other disease parameters measured. Our study demonstrates that controlled-environmental conditions are suitable for rapid identification of resistant genotypes and that genotypes with high levels of resistance can be reliably identified at the cotyledonary, seedling, or flowering stages.

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