scholarly journals Development of a Quick Quantitative Real-Time PCR for the In Vivo Detection and Quantification of Peach latent mosaic viroid

Plant Disease ◽  
2011 ◽  
Vol 95 (2) ◽  
pp. 137-142 ◽  
Author(s):  
Olivier Parisi ◽  
Philippe Lepoivre ◽  
M. Haissam Jijakli
Keyword(s):  
Real Time ◽  
Plant Pathogens ◽  
Low Cost ◽  
Extraction Procedure ◽  
Reaction Conditions ◽  
Routine Testing ◽  
In Vivo Detection ◽  
Peach Trees

Viroids are plant pathogens infecting a broad range of herbaceous and tree crops. Among them, the Peach latent mosaic viroid (PLMVd) infects mainly peach trees, causing a loss of production with no curative options. Detecting this viroid is thus important for certification procedures aiming to avoid the release of infected material into orchards. Presented here is a complete detection method based on reverse transcription (RT) followed by a quantitative real-time polymerase chain reaction (PCR). New primers were selected and optimal reaction conditions determined for routine application of the method. The technique is 105 times more sensitive than the endpoint RT-PCR used for PLMVd detection, and permits earlier detection of PLMVd in infected plants. The quick, low-cost extraction procedure used and the quality of the results obtained make this method suitable for routine testing.

Voltammetric Detection of Tetrodotoxin Real-Time In Vivo of Mouse Organs using DNA-Immobilized Carbon Nanotube Sensors

2019 ◽  
Vol 15 (5) ◽  
pp. 567-574
Author(s):  
Huck Jun Hong ◽  
Suw Young Ly
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
Anticancer Agents ◽  
Cancer Metastasis ◽  
Anodic Stripping Voltammetry ◽  
Stripping Voltammetry ◽  
Takifugu Rubripes ◽  
In Vivo Detection ◽  
Voltammetric Detection

Background: Tetrodotoxin (TTX) is a biosynthesized neurotoxin that exhibits powerful anticancer and analgesic abilities by inhibiting voltage-gated sodium channels that are crucial for cancer metastasis and pain delivery. However, for the toxin’s future medical applications to come true, accurate, inexpensive, and real-time in vivo detection of TTX remains as a fundamental step. Methods: In this study, highly purified TTX extracted from organs of Takifugu rubripes was injected and detected in vivo of mouse organs (liver, heart, and intestines) using Cyclic Voltammetry (CV) and Square Wave Anodic Stripping Voltammetry (SWASV) for the first time. In vivo detection of TTX was performed with auxiliary, reference, and working herring sperm DNA-immobilized carbon nanotube sensor systems. Results: DNA-immobilization and optimization of amplitude (V), stripping time (sec), increment (mV), and frequency (Hz) parameters for utilized sensors amplified detected peak currents, while highly sensitive in vivo detection limits, 3.43 µg L-1 for CV and 1.21 µg L-1 for SWASV, were attained. Developed sensors herein were confirmed to be more sensitive and selective than conventional graphite rodelectrodes modified likewise. A linear relationship was observed between injected TTX concentration and anodic spike peak height. Microscopic examination displayed coagulation and abnormalities in mouse organs, confirming the powerful neurotoxicity of extracted TTX. Conclusion: These results established the diagnostic measures for TTX detection regarding in vivo application of neurotoxin-deviated anticancer agents and analgesics, as well as TTX from food poisoning and environmental contamination.

scholarly journals Real‐Time In Vivo Detection of Cellular Senescence through the Controlled Release of the NIR Fluorescent Dye Nile Blue

2020 ◽  
Vol 59 (35) ◽  
pp. 15152-15156 ◽  
Author(s):  
Beatriz Lozano‐Torres ◽  
Juan F. Blandez ◽  
Irene Galiana ◽  
Alba García‐Fernández ◽  
María Alfonso ◽  
...  
Keyword(s):  
Controlled Release ◽  
Real Time ◽  
Cellular Senescence ◽  
Nile Blue ◽  
Fluorescent Dye ◽  
In Vivo Detection

scholarly journals Optimizing the Reaction Conditions for the Formation of Fumarate via Trans-Hydrogenation

2021 ◽  
Author(s):  
Laura Wienands ◽  
Franziska Theiß ◽  
James Eills ◽  
Lorenz Rösler ◽  
Stephan Knecht ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Thermal Equilibrium ◽  
Reaction Rate ◽  
Low Cost ◽  
Hydrogen Gas ◽  
Metabolic Imaging ◽  
Reaction Conditions ◽  
Precursor Molecule ◽  
Solution Preparation

AbstractParahydrogen-induced polarization is a hyperpolarization method for enhancing nuclear magnetic resonance signals by chemical reactions/interactions involving the para spin isomer of hydrogen gas. This method has allowed for biomolecules to be hyperpolarized to such a level that they can be used for real time in vivo metabolic imaging. One particularly promising example is fumarate, which can be rapidly and efficiently hyperpolarized at low cost by hydrogenating an acetylene dicarboxylate precursor molecule using parahydrogen. The reaction is relatively slow compared to the timescale on which the hyperpolarization relaxes back to thermal equilibrium, and an undesirable 2nd hydrogenation step can convert the fumarate into succinate. To date, the hydrogenation chemistry has not been thoroughly investigated, so previous work has been inconsistent in the chosen reaction conditions in the search for ever-higher reaction rate and yield. In this work we investigate the solution preparation protocols and the reaction conditions on the rate and yield of fumarate formation. We report conditions to reproducibly yield over 100 mM fumarate on a short timescale, and discuss aspects of the protocol that hinder the formation of fumarate or lead to irreproducible results. We also provide experimental procedures and recommendations for performing reproducible kinetics experiments in which hydrogen gas is repeatedly bubbled into an aqueous solution, overcoming challenges related to the viscosity and surface tension of the water.

scholarly journals REAL-TIME ENVIRONMENTAL SENSORS TO IMPROVE HEALTH IN THE SENSING CITY

2016 ◽  
Vol XLI-B2 ◽  
pp. 729-733 ◽  
Author(s):  
L. Marek ◽  
M. Campbell ◽  
M. Epton ◽  
M. Storer ◽  
S. Kingham
Keyword(s):  
Quality Of Life ◽  
Air Pollution ◽  
Air Quality ◽  
Real Time ◽  
Low Cost ◽  
Monitoring Network ◽  
Environmental Data ◽  
Chronic Obstructive ◽  
Actual Usage

The opportunity of an emerging smart city in post-disaster Christchurch has been explored as a way to improve the quality of life of people suffering Chronic Obstructive Pulmonary Disease (COPD), which is a progressive disease that affects respiratory function. It affects 1 in 15 New Zealanders and is the 4th largest cause of death, with significant costs to the health system. While, cigarette smoking is the leading cause of COPD, long-term exposure to other lung irritants, such as air pollution, chemical fumes, or dust can also cause and exacerbate it. Currently, we do know little what happens to the patients with COPD after they leave a doctor’s care. By learning more about patients’ movements in space and time, we can better understand the impacts of both the environment and personal mobility on the disease. This research is studying patients with COPD by using GPS-enabled smartphones, combined with the data about their spatiotemporal movements and information about their actual usage of medication in near real-time. We measure environmental data in the city, including air pollution, humidity and temperature and how this may subsequently be associated with COPD symptoms. In addition to the existing air quality monitoring network, to improve the spatial scale of our analysis, we deployed a series of low-cost Internet of Things (IoT) air quality sensors as well. The study demonstrates how health devices, smartphones and IoT sensors are becoming a part of a new health data ecosystem and how their usage could provide information about high-risk health hotspots, which, in the longer term, could lead to improvement in the quality of life for patients with COPD.

scholarly journals Real‐Time In Vivo Detection of Cellular Senescence through the Controlled Release of the NIR Fluorescent Dye Nile Blue

2020 ◽  
Vol 132 (35) ◽  
pp. 15264-15268
Author(s):  
Beatriz Lozano‐Torres ◽  
Juan F. Blandez ◽  
Irene Galiana ◽  
Alba García‐Fernández ◽  
María Alfonso ◽  
...  
Keyword(s):  
Controlled Release ◽  
Real Time ◽  
Cellular Senescence ◽  
Nile Blue ◽  
Fluorescent Dye ◽  
In Vivo Detection

scholarly journals Real-Time Wireless Platform for In Vivo Monitoring of Bone Regeneration

Sensors ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 4591 ◽  
Author(s):  
Pablo Blázquez-Carmona ◽  
Manuel Sanchez-Raya ◽  
Juan Mora-Macías ◽  
Juan Antonio Gómez-Galán ◽  
Jaime Domínguez ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Real Time ◽  
Ex Vivo ◽  
Low Cost ◽  
Bone Lengthening ◽  
Bone Callus ◽  
Reliable Solution ◽  
Regeneration Processes

For the monitoring of bone regeneration processes, the instrumentation of the fixation is an increasingly common technique to indirectly measure the evolution of bone formation instead of ex vivo measurements or traditional in vivo techniques, such as X-ray or visual review. A versatile instrumented external fixator capable of adapting to multiple bone regeneration processes was designed, as well as a wireless acquisition system for the data collection. The design and implementation of the overall architecture of such a system is described in this work, including the hardware, firmware, and mechanical components. The measurements are conditioned and subsequently sent to a PC via wireless communication to be in vivo displayed and analyzed using a developed real-time monitoring application. Moreover, a model for the in vivo estimation of the bone callus stiffness from collected data was defined. This model was validated in vitro using elastic springs, reporting promising results with respect to previous equipment, with average errors and uncertainties below 6.7% and 14.04%. The devices were also validated in vivo performing a bone lengthening treatment on a sheep metatarsus. The resulting system allowed the in vivo mechanical characterization of the bone callus during experimentation, providing a low-cost, simple, and highly reliable solution.

scholarly journals In vivo real-time recording of UV-induced changes in the autofluorescence of a melanin-containing fungus using a micro-spectrofluorimeter and a low-cost webcam

2009 ◽  
Vol 17 (25) ◽  
pp. 22735 ◽  
Author(s):  
V. Raimondi ◽  
G. Agati ◽  
G. Cecchi ◽  
I. Gomoiu ◽  
D. Lognoli ◽  
...  
Keyword(s):  
Real Time ◽  
Low Cost ◽  
Induced Changes

scholarly journals Real-Time in Vivo Detection of H2O2 Using Hyperpolarized 13C-Thiourea

2017 ◽  
Vol 12 (7) ◽  
pp. 1737-1742 ◽  
Author(s):  
Arif Wibowo ◽  
Jae Mo Park ◽  
Shie-Chau Liu ◽  
Chaitan Khosla ◽  
Daniel M. Spielman
Keyword(s):  
Real Time ◽  
Hyperpolarized 13C ◽  
In Vivo Detection

scholarly journals Detailed imaging of mitochondrial transport and precise manipulation of mitochondrial function with genetically-encoded photosensitizers in adult Drosophila neurons

2021 ◽  
Author(s):  
Francesca Mattedi ◽  
George Chennell ◽  
Alessio Vagnoni
Keyword(s):  
Real Time ◽  
Mitochondrial Function ◽  
Super Resolution ◽  
Neuronal Homeostasis ◽  
Regulation Of Transport ◽  
Detailed Imaging ◽  
Precise Distribution ◽  
Adult Drosophila

Abstract Precise distribution of mitochondria is essential for maintaining neuronal homeostasis. Although detailed mechanisms governing the transport of mitochondria have emerged, it is still poorly understood how the regulation of transport is coordinated in space and time within the physiological context of an organism. How alteration in mitochondrial functionality may trigger changes in organellar dynamics also remains unclear in this context. Therefore, the use of genetically-encoded tools to perturb mitochondrial functionality in real time would be desirable. Here we describe methods to interfere with mitochondrial function with high spatiotemporal precision with the use of photosensitisers in vivo in the intact wing nerve of adult Drosophila. We also provide details on how to visualise the transport of mitochondria and to improve the quality of the imaging to attain super-resolution in this tissue.

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