scholarly journals Dynamics of Race Structures of Pyricularia oryzae Populations Across 18 Seasons in Guangdong Province, China

Plant Disease ◽  
2021 ◽  
Vol 105 (1) ◽  
pp. 144-148
Author(s):  
Zhipeng Huang ◽  
Jinyan Wang ◽  
Yaling Zhang ◽  
Yongxiang Yao ◽  
Lifei Huang ◽  
...  
Keyword(s):  
Rice Blast ◽  
Diversity Index ◽  
Guangdong Province ◽  
Frequency Parameter ◽  
Northeastern China ◽  
Pyricularia Oryzae ◽  
Dynamic Resistance ◽  
Japonica Rice ◽  
Improved Cultivars ◽  
Open Access Article

Rice blast, caused by Pyricularia oryzae, is one of the most damaging fungal diseases affecting rice. Understanding how the pathogen’s race structure varies over time supports the efforts of rice breeders to develop improved cultivars. Here, the race structure of P. oryzae in Guangdong province, China, where rice is cropped twice per year, was assessed over 18 seasons from 1999 through 2008. The analysis was based on the reactions of a panel of seven differential Chinese cultivars to inoculation with a set of 1,248 isolates of P. oryzae in the province. The “total race frequency” parameter ranged from 14.7 to 39.7%, and the “race diversity index” ranged from 0.63 to 0.93. Twelve (ZA63, ZA31, ZA29, ZA21, ZA13, ZA9, ZB30, ZB17, ZB8, ZB2, ZC14, and ZC8) and two (ZD8 and ZD3) races were recognized as specific to indica and japonica rice types, respectively. Of the 59 distinct races identified, only two indica type races (ZC13 and ZC15) were identified as population-common, and nine indica type races (ZB1, ZB5, ZB6, ZB7, ZB13, ZB15, ZC5, ZC13, and ZC15) and one japonica type race (ZG1) were deemed to be population-dominant; the “total top two race isolate frequency” parameter ranged from 29.8 to 74.5%. On the host side, dynamics of resistance structures of the differential set were divided into three patterns: Both Tetep and Kanto 51 expressed the highest and most stable resistance, both Sifeng 43 and Lijiangxintuanheigu conveyed much lower and unstable resistance, and Zhenlong 13, Dongnong 363, and Heijiang 18 performed intermediate and seasonally dynamic resistance. Three interesting points distinguishing race structures of P. oryzae populations in southern and northeastern China were also discussed. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

scholarly journals Genome-wide association of rice response to blast fungus identifies loci for robust resistance under high nitrogen

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mathias Frontini ◽  
Arnaud Boisnard ◽  
Julien Frouin ◽  
Malika Ouikene ◽  
Jean Benoit Morel ◽  
...  
Keyword(s):  
Nitrogen Fertilization ◽  
Rice Blast ◽  
Genome Wide Association ◽  
Japonica Rice ◽  
High Nitrogen ◽  
Initial Finding ◽  
Blast Fungus ◽  
Genome Wide ◽  
A Genome ◽  
Low Nitrogen

Abstract Background Nitrogen fertilization is known to increase disease susceptibility, a phenomenon called Nitrogen-Induced Susceptibility (NIS). In rice, this phenomenon has been observed in infections with the blast fungus Magnaporthe oryzae. A previous classical genetic study revealed a locus (NIS1) that enhances susceptibility to rice blast under high nitrogen fertilization. In order to further address the underlying genetics of plasticity in susceptibility to rice blast after fertilization, we analyzed NIS under greenhouse-controlled conditions in a panel of 139 temperate japonica rice strains. A genome-wide association analysis was conducted to identify loci potentially involved in NIS by comparing susceptibility loci identified under high and low nitrogen conditions, an approach allowing for the identification of loci validated across different nitrogen environments. We also used a novel NIS Index to identify loci potentially contributing to plasticity in susceptibility under different nitrogen fertilization regimes. Results A global NIS effect was observed in the population, with the density of lesions increasing by 8%, on average, under high nitrogen fertilization. Three new QTL, other than NIS1, were identified. A rare allele of the RRobN1 locus on chromosome 6 provides robust resistance in high and low nitrogen environments. A frequent allele of the NIS2 locus, on chromosome 5, exacerbates blast susceptibility under the high nitrogen condition. Finally, an allele of NIS3, on chromosome 10, buffers the increase of susceptibility arising from nitrogen fertilization but increases global levels of susceptibility. This allele is almost fixed in temperate japonicas, as a probable consequence of genetic hitchhiking with a locus involved in cold stress adaptation. Conclusions Our results extend to an entire rice subspecies the initial finding that nitrogen increases rice blast susceptibility. We demonstrate the usefulness of estimating plasticity for the identification of novel loci involved in the response of rice to the blast fungus under different nitrogen regimes.

scholarly journals Bacillus Cyclic Lipopeptides Iturin and Fengycin Control Rice Blast Caused by Pyricularia oryzae in Potting and Acid Sulfate Soils by Direct Antagonism and Induced Systemic Resistance

2021 ◽  
Vol 9 (7) ◽  
pp. 1441
Author(s):  
Van Bach Lam ◽  
Thibault Meyer ◽  
Anthony Arguelles Arias ◽  
Marc Ongena ◽  
Feyisara Eyiwumi Oni ◽  
...  
Keyword(s):  
Rice Blast ◽  
Induced Systemic Resistance ◽  
Pyricularia Oryzae ◽  
Blast Disease ◽  
Systemic Resistance ◽  
Acid Sulfate Soils ◽  
Acid Sulfate ◽  
Wide Range ◽  
Bacillus Spp ◽  
Sulfate Soils

Rice monoculture in acid sulfate soils (ASSs) is affected by a wide range of abiotic and biotic constraints, including rice blast caused by Pyricularia oryzae. To progress towards a more sustainable agriculture, our research aimed to screen the biocontrol potential of indigenous Bacillus spp. against blast disease by triggering induced systemic resistance (ISR) via root application and direct antagonism. Strains belonging to the B. altitudinis and B. velezensis group could protect rice against blast disease by ISR. UPLC–MS and marker gene replacement methods were used to detect cyclic lipopeptide (CLiP) production and construct CLiPs deficient mutants of B. velezensis, respectively. Here we show that the CLiPs fengycin and iturin are both needed to elicit ISR against rice blast in potting soil and ASS conditions. The CLiPs surfactin, iturin and fengycin completely suppressed P. oryzae spore germination resulting in disease severity reduction when co-applied on rice leaves. In vitro microscopic assays revealed that iturin and fengycin inhibited the mycelial growth of the fungus P. oryzae, while surfactin had no effect. The capacity of indigenous Bacillus spp. to reduce rice blast by direct and indirect antagonism in ASS conditions provides an opportunity to explore their usage for rice blast control in the field.

scholarly journals Structural Investigation and Molecular Modeling Studies of Strobilurin-Based Fungicides Active against the Rice Blast Pathogen Pyricularia oryzae

2021 ◽  
Vol 22 (7) ◽  
pp. 3731
Author(s):  
Andrea Kunova ◽  
Luca Palazzolo ◽  
Fabio Forlani ◽  
Giorgia Catinella ◽  
Loana Musso ◽  
...  
Keyword(s):  
Rice Blast ◽  
Rational Design ◽  
Structural Investigation ◽  
Resistance Mechanism ◽  
Three Dimensional ◽  
Pyricularia Oryzae ◽  
Cytochrome Bc1 ◽  
Cytochrome Bc1 Complex ◽  
Three Dimensional Model ◽  
Resistant Strains

The increasing emergence of fungicide-resistant pathogens requires urgent solutions for crop disease management. Here, we describe a structural investigation of new fungicides obtained by combining strobilurin and succinate dehydrogenase inhibitor pharmacophores. We identified compounds endowed with very good activity against wild-type Pyricularia oryzae, combined in some cases with promising activity against strobilurin-resistant strains. The first three-dimensional model of P. oryzae cytochrome bc1 complex containing azoxystrobin as a ligand was developed. The model was validated with a set of commercially available strobilurins, and it well explains both the resistance mechanism to strobilurins mediated by the mutation G143A and the activity of metyltetraprole against strobilurin-resistant strains. The obtained results shed light on the key recognition determinants of strobilurin-like derivatives in the cytochrome bc1 active site and will guide the further rational design of new fungicides able to overcome resistance caused by G143A mutation in the rice blast pathogen.

scholarly journals Glycerol-3-Phosphate Shuttle Is Involved in Development and Virulence in the Rice Blast Fungus Pyricularia oryzae

2018 ◽  
Vol 9 ◽  
Author(s):  
Yongkai Shi ◽  
Huan Wang ◽  
Yuxin Yan ◽  
Huijuan Cao ◽  
Xiaohong Liu ◽  
...  
Keyword(s):  
Rice Blast ◽  
Rice Blast Fungus ◽  
Pyricularia Oryzae ◽  
Blast Fungus

scholarly journals Identificação de progênies de arroz resistentes à brusone no Estado de São Paulo, em 1976/77

Bragantia ◽  
1985 ◽  
Vol 44 (1) ◽  
pp. 311-329
Author(s):  
Jaciro Soave ◽  
Luiz Ernesto Azzini ◽  
Octávio Bento de Almeida Camargo ◽  
Armando Pettinelli Júnior ◽  
Mauro Sakai
Keyword(s):  
Rice Blast ◽  
Sao Paulo ◽  
Pyricularia Oryzae ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
São Paulo

Este trabalho apresenta os resultados das pesquisas realizadas para a avaliação da resistência à brusone (Pyricularia oryzae Cav.) dos principais materiais de sequeiro e irrigado do programa de melhoramento genético do Instituto Agronômico do Estado de São Paulo, e de genótipos exóticos, introduzidos de diversos países, visando à obtenção de cultivares de arroz resistentes àquela limitante doença fúngica. Os testes foram realizados em condições de campo, em canteiros padronizados para reação uniforme a P. oryzae, e a avaliação das plantas foi feita através da observação dos sintomas visuais deixados pela doença, aos quais foram atribuídas notas de 1 a 7, conforme a escala de notas adotadas no "Symposium on the rice blast disease", em 1963. Sessenta e três germoplasmas de arroz de sequeiro e trinta de cultivo irrigado foram testados quanto à resistência à brusone na folha, nas seguintes localidades paulistas: Itararé, Mococa, Pariquera-Açu, Pindamonhangaba, Pindorama e Ribeirão Preto. Foram ainda avaliados 102 genótipos exóticos de arroz visando à detecção de fontes de resistência à brusone nas mesmas localidades, além de Campinas. Somente cinco cultivares de sequeiro, GS-73-164, GS-73-165, GS-73-94, IAC-25 e GS-73-17, e dois cultivares de arroz irrigado, IAC-120 e Pinda F-3-7, embora suscetíveis, apresentaram comportamento satisfatório quanto à brusone. Dos genótipos exóticos testados, vinte e sete foram indicados como fontes de resistência à brusone no Estado de São Paulo.

scholarly journals Level of Endogenous Jasmonate is Critical for Durable Broad-Spectrum Disease Resistance Against Rice Blast in a Japonica Rice Variety, Ziyu44

2021 ◽  
Author(s):  
Xingyu An ◽  
Hui Zhang ◽  
Jinlu Li ◽  
Rui Yang ◽  
Qianchun Zeng ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Signaling Pathway ◽  
Broad Spectrum ◽  
Rice Blast ◽  
Blast Resistance ◽  
Rice Variety ◽  
Blast Disease ◽  
Japonica Rice ◽  
Mycelium Growth ◽  
Broad Spectrum Resistance

Abstract Background: The molecular mechanism of durable and broad-spectrum resistance to rice blast disease in japonica rice variety is still very little known. Ziyu44, a local japonica rice variety in Yunnan Province of China, has shown durable broad-spectrum blast resistance for more than 30 years, and provides an opportunity for us to explore the molecular basis of broad-spectrum resistance to rice blast in japonica rice variety.Methods and Results: We conducted a comparative study of mycelium growth, aposporium formation, the accumulation of salicylate(SA), jasmonate(JA) and H2O2, the expression of SA- and JA-associated genes between Ziyu44 and susceptible variety Jiangnanxiangnuo (JNXN) upon M. oryzae infection. We found that appressorium formation and invasive hyphae extention were greatly inhibited in Ziyu 44 leaves compared with that in JNXN leaves. Both Ziyu 44 and JNXN plants maintained high levels of baseline SA and did not show increased accumulation of SA after inoculation with M. oryzae, while the levels of baseline JA in Ziyu 44 and JNXN plants were relatively low, and the accumulation of JA exhibited markedly increased in Ziyu 44 plants upon M. oryzae infection. The expression levels of key genes involving JA and SA signaling pathway OsCOI1b, OsNPR1, OsMPK6 as well as pathogenesis-related (PR) genes OsPR1a, OsPR1b and OsPBZ1, were markedly up-regulated in Ziyu44. Conclusions: The level of endogenous JA is critical for synchronous activation of SA and JA signaling pathway, up-regulating PR gene expression and enhancing disease resistance against rice blast in Ziyu44.

scholarly journals Evaluation of Rice Responses to the Blast Fungus Magnaporthe oryzae at Different Growth Stages

Plant Disease ◽  
2019 ◽  
Vol 103 (1) ◽  
pp. 132-136 ◽  
Author(s):  
Xinglong Chen ◽  
Yulin Jia ◽  
Bo Ming Wu
Keyword(s):  
Disease Severity ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Growth Stage ◽  
Disease Index ◽  
Blast Disease ◽  
Growth Stages ◽  
Japonica Rice ◽  
Rice Cultivars ◽  
Different Growth Stages

Rice blast, caused by the fungus Magnaporthe oryzae, is the most damaging disease for rice worldwide. However, the reactions of rice to M. oryzae at different growth stages are largely unknown. In the present study, two temperate japonica rice cultivars, M-202 and Nipponbare, were inoculated synchronously at different vegetative growth stages, V1 to V10. Plants of M-202 at each stage from V1 to reproductive stage R8 were inoculated with M. oryzae race (isolate) IB-49 (ZN61) under controlled conditions. Disease reactions were recorded 7 days postinoculation by measuring the percentage of diseased area of all leaves, excluding the youngest leaf. The results showed that the plants were significantly susceptible at the V1 to V4 stages with a disease severity of 26.7 to 46.8% and disease index of 18.62 to 37.76 for M-202. At the V1 to V2 stages, the plants were significantly susceptible with a disease a severity of 28.6 to 39.3% and disease index of 23.65 to 29.82 for Nipponbare. Similar results were observed when plants of M-202 were inoculated at each growth stage with a disease severity of 29.7 to 60.6% and disease index of 21.93 to 59.25 from V1 to V4. Susceptibility decreased after the V5 stage (severity 4.6% and index 2.17) and became completely resistant at the V9 to V10 stages and after the reproductive stages, suggesting that plants have enhanced disease resistance at later growth stages. These findings are useful for managing rice blast disease in commercial rice production worldwide.

The P5-type ATPase Spf1 is required for development and virulence of the rice blast fungus Pyricularia oryzae

2019 ◽  
Vol 66 (2) ◽  
pp. 385-395 ◽  
Author(s):  
Yingmin Qu ◽  
Jing Wang ◽  
Xueming Zhu ◽  
Bo Dong ◽  
Xiaohong Liu ◽  
...  
Keyword(s):  
Rice Blast ◽  
Rice Blast Fungus ◽  
Pyricularia Oryzae ◽  
Blast Fungus
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