scholarly journals Identification of Two Major Rice Sheath Blight Resistance QTLs, qSB1-1HJX74 and qSB11HJX74, in Field Trials Using Chromosome Segment Substitution Lines

Plant Disease ◽  
2014 ◽  
Vol 98 (8) ◽  
pp. 1112-1121 ◽  
Author(s):  
Yajun Zhu ◽  
Shimin Zuo ◽  
Zongxiang Chen ◽  
Xingguang Chen ◽  
Gang Li ◽  
...  
Keyword(s):  
Agronomic Traits ◽  
Field Tests ◽  
Field Trials ◽  
Sheath Blight ◽  
Chromosome Segment ◽  
Substitution Lines ◽  
Resistance Qtls ◽  
Chromosome Segment Substitution Lines ◽  
Segment Substitution ◽  
Moderate Resistance

Sheath blight (SB) is among the most destructive rice (Oryza sativa) diseases worldwide. SB resistance (SBR) is controlled by quantitative trait loci (QTL). Only a few SB resistance QTLs were confirmed previously in field trials that were independent of morphological traits, a crucial factor in plant breeding. Here, we employed 63 chromosome segment substitution lines (CSSLs) to identify SBR QTLs derived from ‘HJX74’. Importantly, these CSSLs all carried the same genetic background as ‘HJX74’, except in the substituted segment introgressed from susceptible ‘Amol3(sona)’. In contrast to most reports that mapped SBR QTLs under complex genetic backgrounds, this approach allowed many CSSLs to consistently retain the agronomic traits of ‘HJX74’ with moderate resistance, giving the needed high reproducibility in SBR scoring. We have identified five SBR QTLs in field tests. Two of them, qSB11HJX74 and qSB1-1HJX74, conferred the greatest reduction in SB ratings by approximately 0.9 to 1.2 on a 0 to 9 scale. qSB11HJX74 exhibited nearly perfect recessive heredity, whereas qSB1-1HJX74 showed dominant heredity. Using a secondary F2 population and overlapping substitution segment lines, we further mapped qSB11HJX74 and qSB1-1HJX74 to regions of approximately 430 and 930 kb, respectively. The results will accelerate the rice breeding process for resistance to SB disease.

scholarly journals Whole-Genome Sequencing of 117 Chromosome Segment Substitution Lines for Genetic Analyses of Complex Traits in Rice

Rice ◽  
2022 ◽  
Vol 15 (1) ◽  
Author(s):  
Jiongjiong Fan ◽  
Hua Hua ◽  
Zhaowei Luo ◽  
Qi Zhang ◽  
Mengjiao Chen ◽  
...  
Keyword(s):  
Complex Traits ◽  
Molecular Breeding ◽  
Agronomic Traits ◽  
Chromosome Segment ◽  
Segment Length ◽  
Whole Genome ◽  
Substitution Lines ◽  
Genetic Analyses ◽  
Chromosome Segment Substitution Lines ◽  
Segment Substitution

AbstractRice is one of the most important food crops in Asia. Genetic analyses of complex traits and molecular breeding studies in rice greatly rely on the construction of various genetic populations. Chromosome segment substitution lines (CSSLs) serve as a powerful genetic population for quantitative trait locus (QTL) mapping in rice. Moreover, CSSLs containing target genomic regions can be used as improved varieties in rice breeding. In this study, we developed a set of CSSLs consisting of 117 lines derived from the recipient ‘Huanghuazhan’ (HHZ) and the donor ‘Basmati Surkb 89–15’ (BAS). The 117 lines were extensively genotyped by whole-genome resequencing, and a high-density genotype map was constructed for the CSSL population. The 117 CSSLs covered 99.78% of the BAS genome. Each line contained a single segment, and the average segment length was 6.02 Mb. Using the CSSL population, we investigated three agronomic traits in Shanghai and Hangzhou, China, and a total of 25 QTLs were detected in both environments. Among those QTLs, we found that RFT1 was the causal gene for heading date variance between HHZ and BAS. RFT1 from BAS was found to contain a loss-of-function allele based on yeast two-hybrid assay, and its causal variation was a P to S change in the 94th amino acid of the RFT1 protein. The combination of high-throughput genotyping and marker-assisted selection (MAS) is a highly efficient way to construct CSSLs in rice, and extensively genotyped CSSLs will be a powerful tool for the genetic mapping of agronomic traits and molecular breeding for target QTLs/genes.

Evaluation of Agronomic Traits in Chromosome Segment Substitution Lines of KDML105 Containing Drought Tolerance QTL under Drought Stress

Rice Science ◽  
2012 ◽  
Vol 19 (2) ◽  
pp. 117-124 ◽  
Author(s):  
Vaiphot KANJOO ◽  
Kanchana PUNYAWAEW ◽  
Jonaliza L. SIANGLIW ◽  
Suwat JEARAKONGMAN ◽  
Apichart VANAVICHIT ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Agronomic Traits ◽  
Chromosome Segment ◽  
Substitution Lines ◽  
Chromosome Segment Substitution Lines ◽  
Segment Substitution

scholarly journals Genetic Dissection of Seed Dormancy using Chromosome Segment Substitution Lines in Rice (Oryza sativa L.)

2020 ◽  
Vol 21 (4) ◽  
pp. 1344 ◽  
Author(s):  
Shaowen Yuan ◽  
Yuntong Wang ◽  
Chaopu Zhang ◽  
Hanzi He ◽  
Sibin Yu
Keyword(s):  
Seed Dormancy ◽  
Genetic Architecture ◽  
Oryza Sativa L ◽  
Expression Profiles ◽  
Chromosome Segment ◽  
Isogenic Line ◽  
Substitution Lines ◽  
Chromosome Segment Substitution Lines ◽  
Segment Substitution ◽  
Seed Dormancy And Germination

Timing of germination determines whether a new plant life cycle can be initiated; therefore, appropriate dormancy and rapid germination under diverse environmental conditions are the most important features for a seed. However, the genetic architecture of seed dormancy and germination behavior remains largely elusive. In the present study, a linkage analysis for seed dormancy and germination behavior was conducted using a set of 146 chromosome segment substitution lines (CSSLs), of which each carries a single or a few chromosomal segments of Nipponbare (NIP) in the background of Zhenshan 97 (ZS97). A total of 36 quantitative trait loci (QTLs) for six germination parameters were identified. Among them, qDOM3.1 was validated as a major QTL for seed dormancy in a segregation population derived from the qDOM3.1 near-isogenic line, and further delimited into a genomic region of 90 kb on chromosome 3. Based on genetic analysis and gene expression profiles, the candidate genes were restricted to eight genes, of which four were responsive to the addition of abscisic acid (ABA). Among them, LOC_Os03g01540 was involved in the ABA signaling pathway to regulate seed dormancy. The results will facilitate cloning the major QTLs and understanding the genetic architecture for seed dormancy and germination in rice and other crops.

Sign in / Sign up

Export Citation Format

Share Document