scholarly journals Transmission of ‘Candidatus Phytoplasma aurantifolia’ to Bakraee (Citrus reticulata Hybrid) by Feral Hishimonus phycitis Leafhoppers in Iran

Plant Disease ◽  
2007 ◽  
Vol 91 (4) ◽  
pp. 466-466 ◽  
Author(s):  
M. Salehi ◽  
K. Izadpanah ◽  
M. Siampour ◽  
A. Bagheri ◽  
S. M. Faghihi

Witches'-broom disease of lime (WBDL) caused by ‘Candidatus Phytoplasma aurantifolia’ is a devastating disease in the Sultanate of Oman, United Arab Emirates, and southern Iran. The disease primarily affects lime (Citrus aurantifolia), but in Iran, it is also found in bakraee, a natural C. reticulata hybrid. The disease has been experimentally transmitted from lime to several citrus cultivars by grafting and to a number of herbaceous hosts by dodder. However, the natural vector of ‘Ca. P. aurantifolia’ has not been determined. The most common phloem-feeding insect associated with lime trees in the area is the leafhopper Hishimonus phycitis. The WBDL phytoplasma has been detected in the body of this leafhopper by ELISA and PCR (1), but previous attempts to establish its vector status have failed. It was recently reported that the leafhopper can release the phytoplasma into a sugar solution by feeding through a Parafilm membrane (4). Here we report successful transmission of WBDL phytoplasma to bakraee seedlings by H. phycitis. The leafhopper nymphs and adults were collected in a WBDL-infected lime orchard in Minab (Hormozgan Province) in May of 2006. Of more than 100 leafhopper samples tested, at least 70% were positive for the phytoplasma by PCR using P1/P7 primer pair (3). Additional field-collected leafhoppers were caged (five per plant) on bakraee seedlings at the two-leaf stage in pots in the greenhouse in Zarghan (Fars Province). After 8 weeks, the remaining leafhoppers were killed with an insecticide. Six months after inoculation, 3 of 10 inoculated plants showed typical symptoms of WBDL, including bud proliferation, general chlorosis, and stunting. Symptomatic plants were strongly positive in PCR assays using primer pair P1/P7. No amplification was obtained with healthy control lime or nonsymptomatic bakraee seedlings. Amplified P1/P7 primed PCR products (1,800 bp) from experimentally vector-challenged bakraee seedlings, captured H. phycitis, and a naturally infected lime tree from Minab were subjected to restriction fragment length polymorphism (RFLP) analysis using AluI, HhaI, HpaII, RsaI, and TaqI enzymes. RFLP patterns from these sources were identical and similar to those reported earlier (2). These analyses verified the identity of WBDL phytoplasma in experimentally infected bakraee seedlings. To our knowledge, this is the first report of natural transmission of ‘Ca. P. aurantifolia’ by H. phycitis. References: (1) J. M. Bové et al. Proc. Conf. IOCV 12:342. 1993. (2) A. J. Khan et al. Phytopathology 92:1038, 2002. (3) B. Schneider et al. Pages 369–380 in: Molecular and Diagnostic Procedures in Mycoplasmology. Vol. 2. S. Razin and J. G. Tully, eds. Academic Press, New York, 1995. (4) M. Siampour et al. Iran. J. Plant Pathol. 41:139 (Farsi) and 35 (English), 2006.

Screen Bodies ◽  
2018 ◽  
Vol 3 (2) ◽  
pp. 86-98
Author(s):  
Josh Morrison ◽  
Sylvie Bissonnette ◽  
Karen J. Renner ◽  
Walter S. Temple

Kate Mondloch, A Capsule Aesthetic: Feminist Materialisms in New Media Art (Minneapolis: University of Minnesota Press, 2018), 151 pp. ISBN: 9781517900496 (paperback, $27) Alberto Brodesco and Federico Giordano, editors, Body Images in the Post-Cinematic Scenario: The Digitization of Bodies (Milan: Mimesis International, 2017). 195 pp., ISBN: 9788869771095 (paperback, $27.50) Cynthia J. Miller and A. Bowdoin Van Riper, editors, What’s Eating You? Food and Horror on Screen (New York: Bloomsbury Academic, 2017). 370pp., ISBN: 9781501322389 (hardback, $105); ISBN: 9781501343964 (paperback, $27.96); ISBN: 9781501322419 (ebook, $19.77) Kaya Davies Hayon, Sensuous Cinema: The Body in Contemporary Maghrebi Cinema (New York: Bloomsbury, 2018). 181pp., ISBN: 9781501335983 (hardback, $107.99)


2020 ◽  
Vol 45 (1) ◽  
pp. 15-30
Author(s):  
Yasuhiro Shimotsuura ◽  
Hiroyuki Maezawa ◽  
Yoshiaki Omura

As Bi-Digital O-Ring Test (originated and founded by Prof. Y. Omura in New York, 1997-2020; follow as BDORT)is a diagnosis method that is carried out on the basic theory of the physiological phenomenon called the decline of muscular power of fingers, the examiner, and patients (or mediator) are demanded to do BDORT by constant regular power. Namely BDORT is a diagnosis method that estimates the relative muscular decline of the patients, so there is such a view that the results of BDORT are reflected by consciousness of the examiner. The authors used the ORT tester by using air system to avoid the influence of electromagnetic wave and evaluated the decline of the muscle strength and open degree of the O-ring shaped by the patients. Patients of the Shimotsuura Clinic are subjected and checked by direct BDORT method. When the patients shapes the O-Ring, staff members stimulated the parts of the body by plastic stick and push foot switch. Decline of the muscle strength & open degree was evaluated. When the open degree was more than 20%, stimulated points were evaluated as abnormal. Opposite side arm of the O-Ring shaped arm was checked as control. The results of the direct BDORT method between ORT evaluation apparatus and the patient was consistent with the results of the indirect method of BDORT method between the doctor and the assistant. Even where the patients complain of ill, the muscle strength was declined and opened the O-Ring by using ORT evaluation apparatus. Especially in the parts of the strong response of Integrin α5β1 checked by the doctor, the muscle strength decreased and the open degree was much higher than other parts of the body. Patients could experience of BDORT by numeral objective evaluation of the decline of the muscle strength by using ORT evaluation apparatus.


1996 ◽  
Vol 37 (2) ◽  
pp. 67-92 ◽  
Author(s):  
David Krasner

Although Aida Overton Walker (1880–1914) belonged to the same generation of turn-of-the-century African American performers as did Bob Cole, J. Rosamond Johnson, Bert Williams, and George Walker, she had a rather different view of how best to represent her race and gender in the performing arts. Walker taught white society in New York City how to do the Cakewalk, a celebratory dance with links to West African festival dance. In Walker's choreography of it, it was reconfigured with some ingenuity to accommodate race, gender, and class identities in an era in which all three were in flux. Her strategy depended on being flexible, on being able to make the transition from one cultural milieu to another, and on adjusting to new patterns of thinking. Walker had to elaborate her choreography as hybrid, merging her interpretation of cakewalking with the preconceptions of a white culture that became captivated by its form. To complicate matters, Walker's choreography developed during a particularly unstable and volatile period. As Anna Julia Cooper remarked in 1892.


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