scholarly journals First Report of Dodder Transmission of ‘Candidatus Liberibacter asiaticus’ to Tomato (Lycopersicon esculentum)

Plant Disease ◽  
2008 ◽  
Vol 92 (5) ◽  
pp. 831-831 ◽  
Author(s):  
Y. P. Duan ◽  
Tim Gottwald ◽  
L. J. Zhou ◽  
D. W. Gabriel
Keyword(s):  
Lycopersicon Esculentum ◽  
Pcr Amplification ◽  
South Florida ◽  
Candidatus Liberibacter Asiaticus ◽  
Tomato Plants ◽  
Murraya Paniculata ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Liberibacter Asiaticus ◽  
Lower Titer

Citrus huanglongbing (HLB) is one of the most devastating diseases of citrus worldwide. The disease is associated with three different species of fastidious α-proteobacteria, namely ‘Candidatus Liberibacter asiaticus’, Ca. L. americanus, and Ca. L. africanus (1). ‘Ca. L. asiaticus’ was first detected in South Florida in 2005 and has spread throughout the citrus-growing areas of Florida. ‘Ca. L. asiaticus’ is transmitted naturally by the Asian citrus psyllid, Diaphorina citri, and can also be transmitted by graft propagation and via various species of dodder (Cuscuta). HLB affects most if not all citrus and citrus relatives within the family Rutaceae (2), including the ornamental shrub Murraya paniculata (4). In addition, ‘Ca. L. asiaticus’ and ‘Ca. L. americanus’ can infect tobacco (Nicotiana xanthi) and periwinkle (Catharanthus roseus) (1,4). Here we report that ‘Ca. L. asiaticus’ can infect tomato (Lycopersicon esculentum) cvs. Manapal and FL47. Manapal and FL 47 plants grown from seed were placed adjacent to ‘Ca. L. asiaticus’-infected sweet orange (Citrus sinensis) plants with dodder (Cuscuta pentagona) already well established on them. Young dodder shoots still connected to the citrus were draped over the tomato plants and subsequently also became attached to the tomato stems. After 1 month, the tomato plants were detached from the citrus and most of the dodder removed. One month later, these tomato plants started to show vein clearing and subsequently the mature leaves became thicker and leathery. Some leaves showed blotchy mottle symptoms and some fruits became lopsided in a manner similar to HLB symptom expression on citrus. PCR amplification of the ‘Ca. L. asiaticus’ 16S rDNA with primers OI1/OI2c and the β-operon with primers A2/J5 (1) revealed the presence of ‘Ca. L. asiaticus’ DNA. Sequence analysis confirmed that the sequences of the cloned amplicons were identical to those from the HLB-infected citrus source plant. Both conventional and quantitative real-time PCR (3) revealed a much lower abundance of ‘Ca. L. asiaticus’ DNA in tomato as compared with ‘Ca. L. asiaticus’-infected citrus or periwinkle, indicating that ‘Ca. L. asiaticus’ bacteria multiplied at a lower titer in these tomato cultivars. References: (1) J. M. Bové, J. Plant Pathol. 88:7, 2006. (2) S. E. Halbert et al. Fla. Entomol. 87:330, 2004. (3) W. Li et al. J. Microbiol. Methods 66:104, 2006. (4) L. Z. Zhou et al. Plant Dis. 91:227, 2007.

Highly sensitive and rapid detection of citrus Huanglongbing pathogen (Candidatus Liberibacter asiaticus) using Cas12a-based methods

2021 ◽  
Author(s):  
Matthew Wheatley ◽  
Yong-Ping Duan ◽  
Yinong Yang
Keyword(s):  
Early Detection ◽  
Nucleic Acids ◽  
Detection Sensitivity ◽  
Sensitive Detection ◽  
Detection Methods ◽  
Candidatus Liberibacter Asiaticus ◽  
Fluorescent Reporter ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Liberibacter Asiaticus

Citrus Huanglongbing (HLB) or greening is one of the most devastating diseases of citrus worldwide. Sensitive detection of its causal agent, Candidatus Liberibacter asiaticus (CLas), is critical for early diagnosis and successful management of HLB. However, current nucleic acid-based detection methods are often insufficient for the early detection of CLas from asymptomatic tissue, and unsuitable for high-throughput and field-deployable diagnosis of HLB. Here we report the development of the Cas12a-based DETECTR (DNA endonuclease-targeted CRISPR trans reporter) assay for highly specific and sensitive detection of CLas nucleic acids from infected samples. The DETECTR assay, which targets the five-copy nrdB gene specific to CLas, couples isothermal amplification with Cas12a trans-cleavage of fluorescent reporter oligos and enables detection of CLas nucleic acids at the attomolar level. The DETECTR assay was capable of specifically detecting the presence of CLas across different infected citrus, periwinkle and psyllid samples, and shown to be compatible with lateral flow assay technology for potential field-deployable diagnosis. The improvements in detection sensitivity and flexibility of the DETECTR technology position the assay as a potentially suitable tool for early detection of CLas in infected regions.

scholarly journals Home Detection Kit for Candidatus Liberibacter asiaticus (LAS) Associated with Citrus Huanglongbing from Psyllids

2014 ◽  
Vol 1 (1) ◽  
Author(s):  
Manjunath L. Keremane ◽  
Chandrika Ramadugu ◽  
Ryo Kubota ◽  
Yongping Duan ◽  
David Hall ◽  
...  
Keyword(s):  
Candidatus Liberibacter Asiaticus ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Liberibacter Asiaticus

Regional Spatial-Temporal Spread of Citrus Huanglongbing Is Affected by Rain in Florida

2018 ◽  
Vol 108 (12) ◽  
pp. 1420-1428 ◽  
Author(s):  
M. M. Shimwela ◽  
T. S. Schubert ◽  
M. Albritton ◽  
S. E. Halbert ◽  
D. J. Jones ◽  
...  
Keyword(s):  
Large Scale ◽  
Disease Incidence ◽  
Kernel Density ◽  
Candidatus Liberibacter Asiaticus ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Density Analysis ◽  
Liberibacter Asiaticus ◽  
Kernel Density Analysis ◽  
Temporal Spread

Citrus huanglongbing (HLB), associated with ‘Candidatus Liberibacter asiaticus’ (Las), disseminated by Asian citrus psyllid (ACP), has devastated citrus in Florida since 2005. Data on HLB occurrence were stored in databases (2005 to 2012). Cumulative HLB-positive citrus blocks were subjected to kernel density analysis and kriging. Relative disease incidence per county was calculated by dividing HLB numbers by relative tree numbers and maximum incidence. Spatiotemporal HLB distributions were correlated with weather. Relative HLB incidence correlated positively with rainfall. The focus expansion rate was 1626 m month−1, similar to that in Brazil. Relative HLB incidence in counties with primarily large groves increased at a lower rate (0.24 year−1) than in counties with smaller groves in hotspot areas (0.67 year−1), confirming reports that large-scale HLB management may slow epidemic progress.

scholarly journals Historical Perspectives, Management, and Current Research of Citrus HLB in Guangdong Province of China, Where the Disease has been Endemic for Over a Hundred Years

2018 ◽  
Vol 108 (11) ◽  
pp. 1224-1236 ◽  
Author(s):  
Zheng Zheng ◽  
Jianchi Chen ◽  
Xiaoling Deng
Keyword(s):  
Southern China ◽  
Candidatus Liberibacter Asiaticus ◽  
Sequencing Technology ◽  
Next Generation Sequencing Technology ◽  
Historical Perspectives ◽  
Citrus Production ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Liberibacter Asiaticus ◽  
Generation Sequencing

Citrus huanglongbing (HLB) is a highly destructive disease currently threatening citrus production worldwide. In China, the disease is exclusively associated with ‘Candidatus Liberibacter asiaticus’, a nonculturable proteobacterium. HLB was observed in Guangdong of China over a hundred years ago. Researchers and citrus growers have been battling with the disease through vigorous research and have exercised various control practices. Much of the early work was not well known outside China. This review is intended to fill in gaps of historical information by reviewing selected literature records. Along the way, the HLB system within southern China was evaluated. Emphases were on comparison of symptomatology, evolution of etiology, control practices, and impacts of using next-generation sequencing technology for ‘Ca. L. asiaticus’ research and detection.

scholarly journals First report on molecular evidence of Candidatus Liberibacter asiaticus associated with citrus Huanglongbing in Venezuela

2020 ◽  
Vol 102 (4) ◽  
pp. 1333-1333
Author(s):  
Edgloris Marys ◽  
Eduardo Rodríguez-Román ◽  
Rafael Mejías ◽  
Alexander Mejías ◽  
Mailyn Mago ◽  
...  
Keyword(s):  
Molecular Evidence ◽  
Candidatus Liberibacter Asiaticus ◽  
First Report ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Liberibacter Asiaticus

scholarly journals Latency and Persistence of ‘Candidatus Liberibacter asiaticus’ in Its Psyllid Vector, Diaphorina citri (Hemiptera: Liviidae)

2017 ◽  
Vol 107 (3) ◽  
pp. 264-272 ◽  
Author(s):  
Maria Cristina Canale ◽  
Arthur Fernando Tomaseto ◽  
Marineia de Lara Haddad ◽  
Helvécio Della Coletta-Filho ◽  
João Roberto Spotti Lopes
Keyword(s):  
Latent Period ◽  
Acquisition Time ◽  
Diaphorina Citri ◽  
Candidatus Liberibacter Asiaticus ◽  
Old Adults ◽  
Sequential Inoculation ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Liberibacter Asiaticus ◽  
Series Of Experiments

Although ‘Candidatus Liberibacter asiaticus’ (Las) is a major pathogen associated with citrus huanglongbing (HLB), some characteristics of transmission by the psyllid vector Diaphorina citri are not fully understood. We examined the latent period and persistence of transmission of Las by D. citri in a series of experiments at 25°C, in which third-instar psyllid nymphs and 1-week-old adults were confined on infected citrus for an acquisition access period (AAP), and submitted to sequential inoculation access periods (IAPs) on healthy citrus seedlings. The median latent period (LP50, i.e., acquisition time after which 50% of the individuals can inoculate) of 16.8 and 17.8 days for psyllids that acquired Las as nymphs and adults, respectively, was determined by transferring single individuals in 48-h IAPs. Inoculation events were intermittent and randomly distributed over the IAPs, but were more frequent after acquisition by nymphs. A minimum latent period of 7 to 10 days was observed by transferring groups of 10 psyllids in 48-h IAPs, after a 96-h AAP by nymphs. Psyllids transmitted for up to 5 weeks, when submitted to sequential 1-week IAPs after a 14-day AAP as nymphs. The long latent period and persistence of transmission are indirect evidences of circulative propagation of Las in D. citri.

scholarly journals Low Incidence of ‘Candidatus Liberibacter asiaticus’ in Murraya paniculata and Associated Diaphorina citri

Plant Disease ◽  
2012 ◽  
Vol 96 (6) ◽  
pp. 827-832 ◽  
Author(s):  
Abigail J. Walter ◽  
David G. Hall ◽  
Yong Ping Duan
Keyword(s):  
Quantitative Polymerase Chain Reaction ◽  
Diaphorina Citri ◽  
Candidatus Liberibacter Asiaticus ◽  
Disease Epidemiology ◽  
Citrus Groves ◽  
Murraya Paniculata ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Low Incidence ◽  
A Minor

Huanglongbing (HLB) is one of the most devastating diseases of citrus worldwide. ‘Candidatus Liberibacter asiaticus’ is the prevalent species of three HLB-associated Liberibacter spp., which is vectored by the psyllid Diaphorina citri. The vector and the bacteria have host plants outside the genus Citrus, and these plants have the potential to affect disease epidemiology within citrus groves. Murraya paniculata could be especially problematic because it is a popular ornamental plant and a host of both psyllid and bacteria. We conducted a year-long survey of eight urban plantings of M. paniculata in east-central Florida to characterize ‘Ca. L. asiaticus’ infection rates in plants and associated psyllids. Using sensitive quantitative polymerase chain reaction (qPCR) primers targeting two prophage genes of ‘Ca. L. asiaticus’, we found infection to be extremely low: less than 1% of psyllids and 1.8% of plants. With qPCR primers targeting ‘Ca. L. asiaticus’ 16S rDNA, none of the plants and only one psyllid were ‘Ca. L. asiaticus’-positive. Therefore, the titer of ‘Ca. L. asiaticus’ is low in M. paniculata and associated psyllids. These results suggest that urban plantings of M. paniculata may serve as a minor source of ‘Ca. L. asiaticus’ inoculum.

scholarly journals Development on Infected Citrus over Generations Increases Vector Infection by ‘Candidatus Liberibacter Asiaticus in Diaphorina citri’

Insects ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 469
Author(s):  
Inaiara de Souza Pacheco ◽  
Diogo Manzano Galdeano ◽  
João Roberto Spotti Lopes ◽  
Marcos Antonio Machado
Keyword(s):  
Management Strategies ◽  
Fourth Generation ◽  
Diaphorina Citri ◽  
Candidatus Liberibacter Asiaticus ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Liberibacter Asiaticus ◽  
Citrus Orchards ◽  
Vector Efficiency ◽  
Control And Eradication

‘Candidatus Liberibacter asiaticus’ (CLas) is a major causal agent of citrus Huanglongbing (HLB), which is transmitted by Asian citrus psyllid (ACP), Diaphorina citri, causing severe losses in various regions of the world. Vector efficiency is higher when acquisition occurs by ACP immature stages and over longer feeding periods. In this context, our goal was to evaluate the progression of CLas population and infection rate over four ACP generations that continuously developed on infected citrus plants. We showed that the frequency of CLas-positive adult samples increased from 42% in the parental generation to 100% in the fourth generation developing on CLas-infected citrus. The bacterial population in the vector also increased over generations. This information reinforces the importance of HLB management strategies, such as vector control and eradication of diseased citrus trees, to avoid the development of CLas-infected ACP generations with higher bacterial loads and, likely, a higher probability of spreading the pathogen in citrus orchards.

scholarly journals Genome Sequence Resource of “Candidatus Liberibacter asiaticus” strain Myan16 from Myanmar

Plant Disease ◽  
2020 ◽  
Author(s):  
Yongqin Zheng ◽  
jun guo ◽  
Xiaoling Deng ◽  
Zheng Zheng
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Sequence Database ◽  
Candidatus Liberibacter Asiaticus ◽  
Genome Sequence Database ◽  
Citrus Production ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Liberibacter Asiaticus

“Candidatus Liberibacter asiaticus” (CaLas), an uncultured α-proteobacterium, is associated with citrus Huanglongbing (HLB, yellow shoot disease), a destructive disease threatening citrus production worldwide. Here, we reported the draft genome sequence of CaLas strain Myan16 from a HLB-affected lime tree in Myitkyina, Kachin State, Myanmar. The strain Myan16 genome is 1,229,102 bp with an average G+C content of 36.4%, along with a circular prophage: P-Myan16-2 (36,303 bp, Type 2). This is the first genome sequence of CaLas strain from Myanmar, which will enrich the current CaLas genome sequence database and facilitate HLB epidemiology research in Asia and world.

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