scholarly journals Molecular Characterization of Sweet potato feathery mottle virus (SPFMV) Isolates from Easter Island, French Polynesia, New Zealand, and Southern Africa

Plant Disease ◽  
2009 ◽  
Vol 93 (9) ◽  
pp. 933-939 ◽  
Author(s):  
M. Rännäli ◽  
V. Czekaj ◽  
R. A. C. Jones ◽  
J. D. Fletcher ◽  
R. I. Davis ◽  
...  

Strains of Sweet potato feathery mottle virus (SPFMV; Potyvirus; Potyviridae) infecting sweet-potato (Ipomoea batatas) in Oceania, one of the worlds' earliest sweetpotato-growing areas, and in southern Africa were isolated and characterized phylogenetically by analysis of the coat protein (CP) encoding sequences. Sweetpotato plants from Easter Island were co-infected with SPFMV strains C and EA. The EA strain isolates from this isolated location were related phylogenetically to those from Peru and East Africa. Sweetpotato plants from French Polynesia (Tahiti, Tubuai, and Moorea) were co-infected with SPFMV strains C, O, and RC in different combinations, whereas strains C and RC were detected in New Zealand. Sweetpotato plants from Zimbabwe were infected with strains C and EA and those from Cape Town, South Africa, with strains C, O, and RC. Co-infections with SPFMV strains and Sweet potato virus G (Potyvirus) were common and, additionally, Sweet potato chlorotic fleck virus (Carlavirus) was detected in a sample from Tahiti. Taken together, occurrence of different SPFMV strains was established for the first time in Easter Island, French Polynesia, and New Zealand, and new strains were detected in Zimbabwe and the southernmost part of South Africa. These results from the Southern hemisphere reflect the anticipated global distribution of strains C, O, and RC but reveal a wider distribution of strain EA than was known previously.

Author(s):  

Abstract A new distribution map is provided for Sweet potato virus G. Potyviridae: Potyvirus. Host: sweet potato (Ipomoea batatas). Information is given on the geographical distribution in Africa (Egypt, Ethiopia, Ghana, Nigeria, South Africa, Tanzania, Zimbabwe), Asia (China, Guangxi, Hainan, Shandong, Sichuan, Indonesia, Japan, Korea Republic, Taiwan), Europe (Spain, Canary Islands), North America (Barbados, USA, Hawaii, Louisiana, Mississippi, North Carolina), Oceania (French Polynesia, New Zealand, Papua New Guinea, Solomon Islands, Timor Leste), South America (Argentina, Brazil, Chile, Peru).


Author(s):  

Abstract A new distribution map is provided for Sweet potato feathery mottle virus (Potyviridae: Potyvirus). Main host: sweet potato (Ipomoea batatas), but also found in many ornamental plants. Information on the geographical distribution in Europe (Italy, Mainland Italy, Portugal, Spain, Canary Islands, Mainland Spain), Asia (China, Anhui, Guangxi, Henan, Jiangsu, Shandong, Yunnan, Zhejiang, East Timor, India, Andhra Pradesh, Kerala, Odisha, West Bengal, Indonesia, Israel, Japan, Kyushu, Ryukyu Archipelago, Korea Republic, Philippines, Syria, Taiwan, Vietnam), Africa (Cameroon, Congo Democratic Republic, Egypt, Ethiopia, Ghana, Kenya, Madagascar, Niger, Nigeria, Rwanda, South Africa, Tanzania, Togo, Uganda, Zambia, Zimbabwe), North America (Canada, Ontario, USA, California, Florida, Kansas, Louisiana, Maryland, Mississippi, North Carolina, Texas), Central America and Caribbean (Costa Rica, Guatemala, Honduras, Puerto Rico), South America (Argentina, Brazil, Goias, Pernambuco, Rio Grande do Sul, Easter Island, Peru, Venezuela) and Oceania (Australia, New South Wales, Northern Territory, Queensland, Western Australia, Fiji, French Polynesia, Guam, New Zealand, Papua New Guinea, Solomon Islands, Tonga) is also given.


Plant Disease ◽  
2007 ◽  
Vol 91 (6) ◽  
pp. 669-676 ◽  
Author(s):  
Milton Untiveros ◽  
Segundo Fuentes ◽  
Luis F. Salazar

Co-infection of Sweet potato chlorotic stunt virus (SPCSV, genus Crinivirus) with Sweet potato feathery mottle virus (SPFMV, genus Potyvirus) results in sweet potato virus disease (SPVD), a synergistic disease that is widely distributed in the sweet potato (Ipomoea batatas) growing regions of the world. Since both SPCSV and SPFMV are common and often detected as part of multiple co-infections of severely diseased plants, the occurrence of synergistic interactions with other viruses was investigated. Data from this study show that SPCSV, but not SPFMV, can cause synergistic diseases in sweet potato with all viruses tested, including members of the genus Potyvirus (Sweet potato latent virus, Sweet potato mild speckling virus), Ipomovirus (Sweet potato mild mottle virus), Cucumovirus (Cucumber mosaic virus), and putative members of the genus Carlavirus (Sweet potato chlorotic fleck virus and C-6 virus). The synergism was expressed as an increase in the severity of symptoms, virus accumulation, viral movement in plants, and as an effect on yield of storage roots. The presence of a third different virus in plants affected with SPVD increased the severity of symptoms even further compared with SPVD alone. There was a positive correlation between increase in virus accumulation and symptom expression in double and triple SPCSV-associated co-infections. The epidemiological implications of the results are discussed.


Author(s):  

Abstract A new distribution map is provided for Sweet potato mild mottle virus, Potyviridae: Ipomovirus. Hosts: sweet potato (Ipomoea batatas). Information is given on the geographical distribution in Africa (Burundi, Kenya, Tanzania, Uganda), Oceania (New Zealand). It is transmitted in a persistent manner by whiteflies, Bemisia tabaci (Hemiptera: Aleyrodidae).


Plant Disease ◽  
2013 ◽  
Vol 97 (9) ◽  
pp. 1260-1260 ◽  
Author(s):  
L.-Y. Wang ◽  
Y.-H. Cheng ◽  
N.-Y. Wang ◽  
K.-C. Chen ◽  
S.-D. Yeh

Sweet potato, Ipomoea batatas (L.) Lam., is an important root crop grown mainly in the counties of Changhua, Yunlin, Tainan, and Pingtung in Taiwan where Sweet potato feathery mottle virus (SPFMV) and Sweet potato latent virus (SPLV) have been reported. Commercial sweet potato grown in Nantou in 2009 and in Hualian in 2010 exhibited downward leaf curling and vein clearing, indicative of viral infection, yet symptoms were distinct from those caused by SPFMV, SPLV, or mixed infection of both viruses. Total RNA was extracted from two symptomatic plants from each county with RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and analyzed by reverse transcription (RT)-PCR using the potyvirusdegenerate primer Hrp5 (1) and oligo-dT18 with BamHI site at the 5′ end (5′-GGATCCTTTTTTTTTTTTTTTTTT-3′). Two healthy plants served as negative controls. An approximately 1.5-kb amplicon covering the region from the 3′-end of the nuclear inclusion protein b (NIb) gene to the 3′-untranslated region (3′-UTR) was amplified from all symptomatic plants, while the healthy controls remained negative. Subsequently, one sample from each location was cloned and sequenced (GenBank Accession No. HQ171932-TW1 [Nantou] and JN205346-TW2 [Hualian]). Based on sequence comparison, the two isolates shared only 86.7% nucleotide identity. BLAST analysis of the CP gene of the isolate TW1 revealed 99% nucleotide identity with the corresponding sequence of Sweet potato virus G (SPVG)-CH2 from China (Z83314). Isolate TW2, however, only shared 86% nucleotide identity with SPVG-CH2, indicating isolate TW2 is genetically different from other isolates and probably represents a new strain of SPVG. The presence of SPVG was further confirmed in symptomatic plants by indirect ELISA using SPVG antiserum developed by Y.-H. Cheng of the Agricultural Research Institute. Since co-infection of different viruses in sweet potato can cause severe leaf symptoms and significant yield reduction (3), a preliminary field survey was also conducted to determine the extent of co-infection with more than one potyvirus using three different primer pairs, SPVGup (5′-ACCGAGCTTTACCCCAGGTAGAGAG-3′)/SPVdw (5′-CGCGCAAGACTCATRTCAGTCAAAT-3′) for SPVG, FM16 (5′-GAATTTAAAGATGCAGGTGTGAAC-3′)/FM895 (5′-GAGGTTATGTATATTTCTAGTAAC-3′) for SPFMV, and L166 (5′-GACAGAGATATCAACACTGGCACC-3′)/L841 (5′-TCCAAGTAGTGTGTGTATGTTCCG-3′) for SPLV. Forty-six of 128 (36%) sweet potato samples collected from Nantou, Hualian, Yunlin, Tainan, and Chiayi counties during 2010 and 2011 tested positive for SPVG. Of the 46 samples that tested positive for SPVG, six were co-infected with SPLV, 19 were co-infected with SPFMV, and two were co-infected with all three viruses. Of the samples that tested negative for SPVG, 10 were infected with SPLV, eight were infected with SPFMV, and two were infected with both SPLV and SPFMV. To date, SPVG has been detected in China, the United States, Peru, Egypt, Ethiopia, Zimbabwe, South Africa, Spain, Java, New Zealand, Hawaii, French Polynesia, and Easter Island (2). To our knowledge, this is the first report of SPVG infecting sweet potato in Taiwan. SPVG could become a new and potentially serious threat to sweet potato production in Taiwan. References: (1) C. C. Chen et al. Bot. Stud. 47:369, 2006. (2) M. Rännäli et al. Plant Dis. 92:1313, 2008. (3) M. Untiveros et al. Plant Dis. 91:669, 2007.


Author(s):  
Willard Mbewe ◽  
Andrew Mtonga ◽  
Margret Chiipanthenga ◽  
Kennedy Masamba ◽  
Gloria Chitedze ◽  
...  

AbstractA survey was carried out in 19 districts to investigate the prevalence and distribution of sweetpotato virus disease (SPVD) and its implication on the sustainability of clean seed system in Malawi. A total of 166 leaf samples were collected and tested for the presence of 8 viruses using nitrocellulose membrane enzyme-linked immunosorbent assay (NCM-ELISA). SPVD foliar symptoms were observed in 68.42% of the surveyed districts. There were significant variations in disease incidence and severity (p < 0.001) among districts, with the highest incidence in Mulanje (28.34%). Average SPVD severity score was 3.05. NCM-ELISA detected sweet potato feathery mottle virus (SPFMV, 30.54%), sweet potato mild mottle virus (SPMMV, 31.14%), sweet potato mild speckling virus (SPMSV, 16.17%), sweet potato C-6 virus (SPC6V, 13.77%), sweet potato chlorotic stunt virus (SPCSV, 22.16%), sweet potato collusive virus (SPCV, 30.54%), sweet potato virus G (SPVG, 11.38%), cucumber mosaic virus (CMV, 7.78%) either in single or mixed infections. Data from this study indicate a significant SPVD occurrence in the country, and the consequence implications towards national sweetpotato seed system.


English Today ◽  
2013 ◽  
Vol 29 (3) ◽  
pp. 60-62
Author(s):  
J. P. Robinson

Eric Partridge's A Dictionary of Slang and Unconventional English, first published in 1937, ran to 8 editions culminating in 1984 and is widely acknowledged as the definitive record of twentieth-century British slang. The New Partridge Dictionary of Slang and Unconventional English (NPD) maintains the tradition impressively, enhanced by a more conventional approach to citing sources, a broader focus to include examples of colloquial and vernacular vocabulary worldwide and prominence given to usage since 1945. A thousand new entries from the UK, USA, Australia, New Zealand, Canada, India, South Africa, Ireland and the Caribbean, and increased representation of the language of social media, document linguistic innovation and/or reflect more sophisticated lexical data capture since the previous print edition of 2006. The 19 pages of introductory text outline criteria for inclusion, describe the structure of entries and provide a fascinating set of observations on slang drawn from Partridge's many published works. With over 60,000 entries the second edition of NPD is complemented for the first time by Partridge Slang Online (PSO), a resource which offers new ways to access and interrogate the data.


2015 ◽  
Vol Volume 111 (Number 11/12) ◽  
Author(s):  
Nozipho M. Motsa ◽  
Albert T. Modi ◽  
Tafadzwanashe Mabhaudhi ◽  
◽  
◽  
...  

Abstract Sweet potato is an important ‘indigenised’ root crop in South Africa. It features prominently in smallholder cropping systems because of its versatility, drought tolerance and positive role in food security. It outranks most staple crops in vitamins, minerals, dietary fibre and protein content. Much information is available on sweet potato as a drought tolerant and food security crop, but critical reviews that link its drought tolerance with food security are lacking. We review sweet potato as a food security crop, focusing on mechanisms associated with drought. We conclude that the crop has great potential in the light of imminent challenges associated with drought as a negative effect of climate change.


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