scholarly journals First Report of Banana Streak Virus Infecting Sugarcane and Arrowroot in Colombia

Plant Disease ◽  
1997 ◽  
Vol 81 (5) ◽  
pp. 552-552 ◽  
Author(s):  
H. Reichel ◽  
S. Belalcázar ◽  
G. Múnera ◽  
E. Arévalo ◽  
J. Narváez
Keyword(s):  
Saccharum Officinarum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Streak Virus ◽  
Banana Streak Virus ◽  
First Report ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Sugarcane Bacilliform Virus ◽  
Foliar Tissue ◽  
Saccharicoccus Sacchari

We have recently reported on the presence of banana streak virus (BSV) affecting plantains (Musa spp.) in Colombia (2). BSV is serologically related to sugarcane bacilliform virus and has been found to be transmitted by the pink mealybug (Saccharicoccus sacchari) from sugarcane to banana (1). In the vicinity of affected plantain crops in the localities of Andes (Antioquia) and Montenegro (Quindio), we observed sugarcane (Saccharum officinarum L.) plants with chlorotic streaks on their leaves, as well as arrowroot (Canna edulis Ker-Gawl.) plants with mild mosaic symptoms. The foliar tissue of symptomatic plants of these two species was tested for BSV and cucumber mosaic virus (CMV) by double antibody sandwich-enzyme-linked immunosorbent assay with commercial polyclonal antisera (Agdia Inc., Elkhart, IN). BSV was detected in samples of both plant species, whereas CMV was not detected in either one. Immunosorbent electron microscopy analysis of BSV-infected, symptomatic, foliar tissue of sugarcane showed the presence of viral-like bacilliform particles measuring approximately 150 × 30 nm, typical of BSV. This is the first report of BSV infecting Saccharum officinarum in Colombia and the first report of Canna edulis as a host for this virus. References: (1) B. E. L. Lockhart and L. J. C. Autrey. Plant Dis. 72:230, 1988. (2) H. Reichel et al. Plant Dis. 80:463, 1996.

scholarly journals Primer Reporte del Virus del Rayado del Banano (BSV) Afectando Plantaciones de Plátano (Musa AAB Simmonds), Caña de azúcar (Sacharum officinarum) y Achira (Canna edulis) en Colombia

1996 ◽  
Vol 1 (1) ◽  
pp. 35 ◽  
Author(s):  
Helena Reichel ◽  
Silvio Belalcázar ◽  
Gladys Múnera ◽  
Emilio Arévalo ◽  
Javier Narváez
Keyword(s):  
Saccharum Officinarum ◽  
Streak Virus ◽  
Banana Streak Virus ◽  
First Report ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Polyclonal Antisera ◽  
Foliar Tissue ◽  
First Time ◽  
Das Elisa

<p>En noviembre de 1995, en los Municipios de Andes, Venecia e Hispania (Antioquia), La Tebaida y Montenegro (Quindío), se observaron hojas de plantas del don Dominico-Hartón (Musa AAB Simmonds) con rayas cloróticas y necróticas, síntomas que caracterizan la enfermedad del rayado del banano. En ocasiones las plantas presentaban síntomas de mosaico en el pseudotallo y el engrosamiento y/o rompimiento de la base del mismo. Además, en las cercanías de las plantaciones de plátano de los Municipios de Andes y Montenegro, se observaron respectivamente plantas de caña de azúcar (Saccharum officinarum) con síntomas de clorosis y de achira (Canna edulis) con síntomas de mosaico leve en sus hojas. Muestras de tejido foliar de éstas tres especies de plantas fueron analizadas para detectar la presencia del badnavirus del rayado del banano (BSV) y del virus del mosaico del pepino (CMV), mediante la prueba serológica DAS-ELISA, empleando anticuerpos policlonales comerciales (AGDIA Inc., Elkhart, IN). En el caso del plátano, en la región de Antioquia únicamente se detectó el BSV en el so% de las plantas sintomáticas analizadas, mientras que en el Quindío, el 6oo/o de las plantas estuvieron infectadas simultáneamente por BSV y CMV. El BSV se detectó también en muestras de tejido foliar de caña de azúcar y achira, pero en ningún caso resultaron positivas para el CMV, según la prueba DAS-ELISA. El análisis mediante microscopía electrónica de immunoabsorbancia (ISEM) del tejido foliar de plátano y caña de azúcar infectado, indicó la presencia de partículas baciliformes típicas del BSV de aproximadamente 30 x 110 nm. Hasta donde conocemos, este es el primer reporte sobre la presencia del BSV afectando plátano, caña de azúcar y achira en Colombia y es la primera vez que se reporta a la achira como hospedero de éste virus en el mundo.</p><p><strong><br /></strong></p><p><strong>First Report of Banana Streak Virus (BSV) Infecting Plantain (Musa AAB Simmonds), Sugar cane (Sacharumofficinarum) and Achira (Canna edulis) in Colombia</strong></p><p>Viruslike symptoms of yellow striate mosaic to necrotic streaks were observed on plantain leaves of the cultivar Dominico-Hartón (Musa AAB Simmonds) in the municipalities of Andes, Venecia and Hispania (Antioquia) and Tebaida and Montenegro (Quindío), Colombia. Symptoms sometimes included mosaic and swelling at the base of the pseudostem. Furthermore, in the neighborhood of the plantain crops, at the localities of Andes and Montenegro, it was observed respectively plants of sugarcane (Saccharum officinarum L.) with chlorotic foliar tissue and of achira (Canna edulis L.) with symptoms of mild mosaic in their leaves. The foliar tissue of symptomatic plants of these three species, was tested for banana streak virus (BSV ) and cucumber mosaic virus (CMV ) by double antibody sandwich enzime-linked immunosorbent assay (DAS-ELISA) with commercial polyclonal antisera (Agdia Inc., Elkhart, IN). BSV was detected in samples of all plant species. In Plantain, so% of the examined plants from the Antioquia region were infected by BSV, but not CMV; whereas, at the Quindío region, 6o% of the plants were simultaneously infected by both viruses, as detected by DAS- ELISA. CMV was not detected in foliar tissue of either sugarcane or achira plants. Immunosorbent electron microscopy analysis (ISEM) of BSV infected foliar tissue of plantain and sugarcane, showed the presence of viral bacilliform particles measuring ca. 30 x 110 nm, typical of BSV. Up to our knowledge, this is the first report of BSV infecting Musa spp., Saccharum officinarum and Canna edulis in Colombia, and the first time that Canna edulis is reported as a host for this virus.</p>

scholarly journals First Report of Banana Streak Virus Infecting Banana Cultivars (Musa spp.) in Taiwan

Plant Disease ◽  
1997 ◽  
Vol 81 (5) ◽  
pp. 550-550 ◽  
Author(s):  
Hong-Ji Su ◽  
Ting-Hsuan Hung ◽  
Meng-Ling Wu
Keyword(s):  
Enzyme Linked Immunosorbent Assay ◽  
Streak Virus ◽  
Banana Streak Virus ◽  
Banana Bunchy Top Virus ◽  
Citrus Mealybug ◽  
First Report ◽  
Musa Spp ◽  
Cavendish Banana ◽  
Southern Taiwan ◽  
Leaf Symptoms

Banana (Musa sapientam L.) is an economically important crop for both export and local consumption in Taiwan. Recently, leaf symptoms characteristic of banana streak disease (1) were found on banana cv. Mysore (AAB group) introduced from Australia in the germ plasm collection of the Taiwan Banana Research Institute. The citrus mealybug (Planococus citri) has been shown to transmit banana streak virus (BSV) but not banana bunchy top virus or cucumber mosaic virus (CMV) (2). When mealybugs were fed on leaves of diseased Mysore banana and transferred to healthy banana cv. Cavendish seedlings in a growth chamber, the latter developed fine chlorotic streaks characteristic of symptoms caused by BSV within 1 to 3 months. Some chlorotic streaks became necrotic. BSV was detected in diseased but not healthy leaves of Mysore and Cavendish bananas by polymerase chain reaction (PCR) with primer pairs of BSV provided by J. E. Thomas of Queensland Department of Primary Industries. Subsequently, fine chlorotic streaks were observed in leaves of Cavendish banana in several fields in southern Taiwan. Some of these diseased plants developed severe leaf necrosis, causing heart rot of spindle leaves characteristic of symptoms caused by CMV. Presence of BSV in these plants was verified by PCR assay. However, CMV was also detected by double antibody sandwich-enzyme-linked immunosorbent assay with a monoclonal antibody to CMV, indicating that these plants were simultaneously infected by both viruses. This is the first report of BSV infecting Musa spp. in Taiwan. References: (1) B. E. L. Lockhart. Phytopathology 76:995, 1986. (2) B. E. L. Lockhart. 1995 Food & Fertilizer Technol. Center (ASPAC) Tech. Bull. 143. 11 pp.

scholarly journals Production of Human Papillomavirus Type 16 L1 Virus-Like Particles by Recombinant Lactobacillus casei Cells

2006 ◽  
Vol 72 (1) ◽  
pp. 745-752 ◽  
Author(s):  
Karina Araujo Aires ◽  
Aurora Marques Cianciarullo ◽  
Sylvia Mendes Carneiro ◽  
Luisa Lina Villa ◽  
Enrique Boccardo ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Lactobacillus Casei ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hpv 16 ◽  
Virus Like Particles ◽  
Human Papillomavirus Type 16 ◽  
Common Causes ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
L1 Protein

ABSTRACT Infections with human papillomavirus type 16 (HPV-16) are closely associated with the development of human cervical carcinoma, which is one of the most common causes of cancer death in women worldwide. At present, the most promising vaccine against HPV-16 infection is based on the L1 major capsid protein, which self-assembles in virus-like particles (VLPs). In this work, we used a lactose-inducible system based on the Lactobacillus casei lactose operon promoter (plac) for expression of the HPV-16 L1 protein in L. casei. Expression was confirmed by Western blotting, and an electron microscopy analysis of L. casei expressing L1 showed that the protein was able to self-assemble into VLPs intracellularly. The presence of conformational epitopes on the L. casei-produced VLPs was confirmed by immunofluorescence using the anti-HPV-16 VLP conformational antibody H16.V5. Moreover, sera from mice that were subcutaneously immunized with L. casei expressing L1 reacted with Spodoptera frugiperda-produced HPV-16 L1 VLPs, as determined by an enzyme-linked immunosorbent assay. The production of L1 VLPs by Lactobacillus opens the possibility for development of new live mucosal prophylactic vaccines.

scholarly journals First Report of Banana Streak Virus Infecting Bananas (Musa spp.) in Georgia, U.S.A.

Plant Disease ◽  
2020 ◽  
Vol 104 (4) ◽  
pp. 1261-1261
Author(s):  
S. Waliullah ◽  
E. G. Fonsah ◽  
P. Ji ◽  
M. E. Ali
Keyword(s):  
Streak Virus ◽  
Banana Streak Virus ◽  
First Report ◽  
Musa Spp

scholarly journals First Report on Molecular Detection of Phytoplasmas in Papaya in Cuba

Plant Disease ◽  
2003 ◽  
Vol 87 (9) ◽  
pp. 1148-1148 ◽  
Author(s):  
Y. Arocha ◽  
D. Horta ◽  
E. Peralta ◽  
P. Jones
Keyword(s):  
Electron Microscopy ◽  
Molecular Detection ◽  
Intergenic Sequence ◽  
Nested Polymerase Chain Reaction ◽  
First Report ◽  
Transmission Electron ◽  
Dehydrogenase Gene ◽  
Fruit Samples ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis

Phytoplasmas and rickettsias have been associated with recent nonviral papaya diseases (1,2). Forty leaf samples with symptoms similar to papaya bunchy top (PBT) (1) and Australian papaya diseases (2) were collected from surveys done in Cuban papaya areas of Havana and Villa Clara provinces. Thirty nine plants showed typical PBT symptoms, while 35 plants also exhibited symptoms previously described for phytoplasma diseases (2) such as yellowing, crinkling, necrosis and deformation of older leaves, phyllody, virescence, short internodes, reduced latex flow, stunted crown leaves, and abscission of the fruit. Samples were evaluated by transmission electron microscopy, nested polymerase chain reaction (nPCR) with 16S ribosomal RNA phytoplasma primers SN910601/DH6-R16F2n/R16R2, PCR with rickettsial succinate dehydrogenase gene (sdhA) primers PBT1/PBT2, HaeIII, RsaI, and AluI enzyme restriction, and phytoplasma intergenic sequence analyses. Rickettsia PCR amplifications of 750 bp were obtained for samples with PBT symptoms, while the 35 phytoplasma-infected samples yielded amplifications of 1,250 bp. Restriction profiles and a 98% homology in the intergenic sequence (GenBank Accession No. AY257547) confirmed the presence of apple proliferation phytoplasma group. Electron microscopy analysis evidenced the presence of particles similar to rickettsia and phytoplasma pleomorfic bodies in more than 50% of samples analysed. Also, those similar to potyvirus and rhabdovirus were observed in 22.5%. To our knowledge, these results are the first report on the molecular detection of phytoplasmas in papaya in Cuba and suggest a possible concomitance among phytopathogens detected. References: (1) M. Davis et al. Curr. Microbiol. 36:80, 1998. (2) K. Gibb et al. Plant Dis. 80:174, 1996.

scholarly journals First Report of Tobacco streak virus Infecting Safflower (Carthamus tinctorius) in Maharashtra, India

Plant Disease ◽  
2003 ◽  
Vol 87 (11) ◽  
pp. 1396-1396 ◽  
Author(s):  
S. Chander Rao ◽  
R. D. V. J. Prasada Rao ◽  
V. Manoj Kumar ◽  
Divya S. Raman ◽  
M. A. Raoof ◽  
...  
Keyword(s):  
Carthamus Tinctorius ◽  
Tobacco Streak Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Streak Virus ◽  
Leaf Extracts ◽  
Systemic Necrosis ◽  
First Report ◽  
Veinal Necrosis ◽  
Terminal Bud ◽  
Leaf Necrosis

Safflower, Carthamus tinctorius L. (Asteraceae), is extensively cultivated in India, China, and other parts of Asia for edible oil, dyeing agent, and its medicinal value. In 2003, safflower entry (NARI-6) in the All-India Coordinated Research Project on Oilseeds (Safflower) grown in the experimental fields of M/s Syngenta India Ltd., Aurangabad (Maharashtra State, India) exhibited symptoms of veinal and leaf necrosis, necrotic streaks on the stem, necrosis of the terminal bud, and ultimately plant death. The disease was attributed to Tobacco streak virus (TSV) because sunflower growing adjacent to safflower showed similar symptoms caused by TSV (1). Mechanical inoculations of sap from symptomatic safflower leaves caused typical symptoms of TSV (local, irregular, necrotic rings, veinal necrosis, and systemic veinal necrosis) on Vigna unguiculata (L.) Walp. cv. C-152 and Phaseolus vulgaris (L.) cv. Topcrop, and symptoms of local, necrotic lesions, veinal necrosis, and systemic necrosis of leaf and growing bud on Arachis hypogaea L. cv. JL-24. Sap-inoculated safflower cv. Manjeera showed chlorotic and necrotic local lesions followed by systemic leaf necrosis, leading to necrosis and death of the terminal bud. Safflower cvs. A-1, BIP-2, Co-1, and Bheema (10 plants of each cultivar) inoculated with sap from safflower plants showing typical TSV symptoms did not show any visible symptoms except stunting, but six to nine plants of each cultivar tested positive for TSV using enzyme-linked immunosorbent assay (ELISA) tests. In direct antigen coating-ELISA, the virus reacted positively with antiserum produced to an isolate of TSV from peanut (2) and to antiserum to TSV (ATCC-PVAS 276 for Datura stramonium), but did not react to peanut bud necrosis tospovirus antiserum. Examination of leaf extracts using leaf-dips and immunosorbent electron microscopy with the antiserum of TSV-peanut isolate showed isometric particles resembling those in the genus Ilarvirus. To our knowledge, this is the first report of an isolate of Tobacco streak virus infecting safflower. References: (1) R. D. V. J. Prasada Rao et al. J. Oilseeds Res. 17:400, 2000. (2) A. S. Reddy et al. Plant Dis. 86:173, 2002.

scholarly journals First Report of Bean pod mottle virus in Soybean in Canada

Plant Disease ◽  
2002 ◽  
Vol 86 (3) ◽  
pp. 330-330 ◽  
Author(s):  
R. Michelutti ◽  
J. C. Tu ◽  
D. W. A. Hunt ◽  
D. Gagnier ◽  
T. R. Anderson ◽  
...  
Keyword(s):  
Soybean Mosaic Virus ◽  
Mottle Virus ◽  
Tobacco Streak Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Streak Virus ◽  
Growth Stages ◽  
Essex County ◽  
Bean Pod Mottle Virus ◽  
First Report ◽  
Soybean Plants

In 2001, soybean fields were surveyed to determine the incidence of viruses because soybean aphids (Aphis glycines Matsamura), known to transmit Soybean mosaic virus (SMV) (2), were found in Ontario. In addition, bean leaf beetle (Cerotoma trifurcata Forster) was found during 2000 to be contaminated with Bean pod mottle virus (BPMV), although soybean plants, on which the beetles were feeding, tested negative (3). In the current survey, young soybean leaves were selected at random in July and August from 20 plants per site at growth stages R4 to R5 (1) from 415 sites representing the entire soybean-producing area in Ontario. Samples were maintained under cool conditions until received at the laboratory, where they were promptly processed. A combined sub-sample was obtained from the 20 plants per site. The 415 sub-samples were tested for SMV, BPMV, Tobacco ringspot virus (TRSV), and Tobacco streak virus (TSV) using polyclonal antibody kits for double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) (Agdia Inc., Elkart, IN). The ELISA plates were read with a plate reader (MRX, Dynex Technologies Inc., Chantilly, VA), and results were analyzed using ELISA software (Leading Edge Research, Merrickville, Ontario) and compared positive and negative controls (Agdia). TRSV was detected in one sample from Essex County and another sample from Middlesex County. SMV, BPMV, and TSV were not found in commercial soybean fields. However, SMV and BPMV were found in samples originating from two soybean breeding nurseries, one in Essex County and one in Kent County. Seedlings of soybean cv. Williams 82 were inoculated in the greenhouse with sap from leaf samples that tested positive for BPMV. Leaves of plants that developed mosaic symptoms were retested using ELISA and confirmed to be positive for BPMV. SMV and TRSV have been found previously in commercial soybean fields in Ontario (4). To our knowledge, this is the first report of BPMV on soybean plants in Canada. References: (1) W. R. Fehr et al. Merr. Crop. Sci. 11:929, 1971. (2) J. H. Hill et al. Plant Dis. 85:561, 2001. (3) A. U. Tenuta. Crop Pest. 5 (11):8, 2000. (4) J. C. Tu. Can. J. Plant Sci. 66:491, 1986.

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