scholarly journals First Report of Banana Streak Virus Infecting Banana Cultivars (Musa spp.) in Taiwan

Plant Disease ◽  
1997 ◽  
Vol 81 (5) ◽  
pp. 550-550 ◽  
Author(s):  
Hong-Ji Su ◽  
Ting-Hsuan Hung ◽  
Meng-Ling Wu
Keyword(s):  
Enzyme Linked Immunosorbent Assay ◽  
Streak Virus ◽  
Banana Streak Virus ◽  
Banana Bunchy Top Virus ◽  
Citrus Mealybug ◽  
First Report ◽  
Musa Spp ◽  
Cavendish Banana ◽  
Southern Taiwan ◽  
Leaf Symptoms

Banana (Musa sapientam L.) is an economically important crop for both export and local consumption in Taiwan. Recently, leaf symptoms characteristic of banana streak disease (1) were found on banana cv. Mysore (AAB group) introduced from Australia in the germ plasm collection of the Taiwan Banana Research Institute. The citrus mealybug (Planococus citri) has been shown to transmit banana streak virus (BSV) but not banana bunchy top virus or cucumber mosaic virus (CMV) (2). When mealybugs were fed on leaves of diseased Mysore banana and transferred to healthy banana cv. Cavendish seedlings in a growth chamber, the latter developed fine chlorotic streaks characteristic of symptoms caused by BSV within 1 to 3 months. Some chlorotic streaks became necrotic. BSV was detected in diseased but not healthy leaves of Mysore and Cavendish bananas by polymerase chain reaction (PCR) with primer pairs of BSV provided by J. E. Thomas of Queensland Department of Primary Industries. Subsequently, fine chlorotic streaks were observed in leaves of Cavendish banana in several fields in southern Taiwan. Some of these diseased plants developed severe leaf necrosis, causing heart rot of spindle leaves characteristic of symptoms caused by CMV. Presence of BSV in these plants was verified by PCR assay. However, CMV was also detected by double antibody sandwich-enzyme-linked immunosorbent assay with a monoclonal antibody to CMV, indicating that these plants were simultaneously infected by both viruses. This is the first report of BSV infecting Musa spp. in Taiwan. References: (1) B. E. L. Lockhart. Phytopathology 76:995, 1986. (2) B. E. L. Lockhart. 1995 Food & Fertilizer Technol. Center (ASPAC) Tech. Bull. 143. 11 pp.

scholarly journals First Report of Banana Streak Virus Infecting Bananas (Musa spp.) in Georgia, U.S.A.

Plant Disease ◽  
2020 ◽  
Vol 104 (4) ◽  
pp. 1261-1261
Author(s):  
S. Waliullah ◽  
E. G. Fonsah ◽  
P. Ji ◽  
M. E. Ali
Keyword(s):  
Streak Virus ◽  
Banana Streak Virus ◽  
First Report ◽  
Musa Spp

scholarly journals Banana Streak Badnavirus and Cucumber Mosaic Cucumovirus in Farmers' Fields in Zanzibar

Plant Disease ◽  
1998 ◽  
Vol 82 (12) ◽  
pp. 1403-1403 ◽  
Author(s):  
D. R. Vuylsteke ◽  
J. d'A. Hughes ◽  
K. Rajab
Keyword(s):  
Electron Microscopy ◽  
Pest Management ◽  
Research Station ◽  
First Report ◽  
Musa Spp ◽  
Cavendish Banana ◽  
Cucumber Mosaic Cucumovirus ◽  
Viruslike Particles ◽  
Banana Streak Badnavirus ◽  
Leaf Symptoms

Symptoms resembling those of viral leaf streak disease, caused by banana streak badnavirus (BSV), were observed in May 1998 on two banana (Musa spp.) landraces grown from farmer-collected propagules in a farmer's field at Kiboje Uchukuni, Zanzibar. Those showing symptoms were “French plantain” cv. Mzuzu and “Cavendish” banana cv. Mtwike. Leaf symptoms were expressed as chlorotic streaks and blotches. Leaf samples were indexed by immunosorbent electron microscopy with BSV and cucumber mosaic cucumovirus (CMV) antibodies, using partially purified preparations (2). The two landraces tested positive for BSV, corroborating the occurrence of BSV in Zanzibar. In addition, cv. Mtwike was found to be coinfected with CMV. No other viruslike particles were seen by electron microscopy. Although BSV has been reported in Zanzibar (1), it was only from symptoms in the Musa field genebank at Kizimbani Research Station. BSV has been found in many Musa collections worldwide, particularly in the widely grown cv. Mysore. This report confirms the presence of BSV in farmers' fields and is also the first report of CMV infecting banana in Zanzibar. References: (1) A. J. Dabek and J. M. Waller. Trop. Pest Management 36:157, 1990. (2) M. Diekmann and C. A. J. Putter. Musa spp. FAO/IPGRI Technical Guidelines for the Safe Movement of Germplasm No. 15. FAO/IPGRI, Rome, Italy, 1996.

scholarly journals First Report of Banana Streak Virus Infecting Sugarcane and Arrowroot in Colombia

Plant Disease ◽  
1997 ◽  
Vol 81 (5) ◽  
pp. 552-552 ◽  
Author(s):  
H. Reichel ◽  
S. Belalcázar ◽  
G. Múnera ◽  
E. Arévalo ◽  
J. Narváez
Keyword(s):  
Saccharum Officinarum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Streak Virus ◽  
Banana Streak Virus ◽  
First Report ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Sugarcane Bacilliform Virus ◽  
Foliar Tissue ◽  
Saccharicoccus Sacchari

We have recently reported on the presence of banana streak virus (BSV) affecting plantains (Musa spp.) in Colombia (2). BSV is serologically related to sugarcane bacilliform virus and has been found to be transmitted by the pink mealybug (Saccharicoccus sacchari) from sugarcane to banana (1). In the vicinity of affected plantain crops in the localities of Andes (Antioquia) and Montenegro (Quindio), we observed sugarcane (Saccharum officinarum L.) plants with chlorotic streaks on their leaves, as well as arrowroot (Canna edulis Ker-Gawl.) plants with mild mosaic symptoms. The foliar tissue of symptomatic plants of these two species was tested for BSV and cucumber mosaic virus (CMV) by double antibody sandwich-enzyme-linked immunosorbent assay with commercial polyclonal antisera (Agdia Inc., Elkhart, IN). BSV was detected in samples of both plant species, whereas CMV was not detected in either one. Immunosorbent electron microscopy analysis of BSV-infected, symptomatic, foliar tissue of sugarcane showed the presence of viral-like bacilliform particles measuring approximately 150 × 30 nm, typical of BSV. This is the first report of BSV infecting Saccharum officinarum in Colombia and the first report of Canna edulis as a host for this virus. References: (1) B. E. L. Lockhart and L. J. C. Autrey. Plant Dis. 72:230, 1988. (2) H. Reichel et al. Plant Dis. 80:463, 1996.

scholarly journals First Report of Tomato Brown Rugose Fruit Virus Infecting Tomato Crop in Saudi Arabia

Plant Disease ◽  
2021 ◽  
Author(s):  
Ahmed Sabra ◽  
Mohammed Ali Al Saleh ◽  
I. M. Alshahwan ◽  
Mahmoud A. Amer
Keyword(s):  
Saudi Arabia ◽  
Mosaic Virus ◽  
Solanum Lycopersicum ◽  
Tomato Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
First Report ◽  
Pcr Products ◽  
Dark Green ◽  
Leaf Symptoms

Tomato (Solanum lycopersicum L.) is the most economically important member of family Solanaceae and cultivated worldwide and one of the most important crops in Saudi Arabia. The aim of this study is screening of the most common viruses in Riyadh region and identified the presence of tomato brown rugose fruit virus (ToBRFV) in Saudi Arabia. In January 2021, unusual fruit and leaf symptoms were observed in several greenhouses cultivating tomatoes commercially in Riyadh Region, Saudi Arabia. Fruit symptoms showed irregular brown spots, deformation, and yellowing spots which render the fruits non-marketable, while the leaf symptoms included mottling, mosaic with dark green wrinkled and narrowing. These plants presented the symptoms similar to those described in other studies (Salem et al., 2015, Luria et al., 2017). A total 45 Symptomatic leaf samples were collected and tested serologically against suspected important tomato viruses including: tomato chlorosis virus, tomato spotted wilt virus, tomato yellow leaf curl virus, tomato chlorotic spot virus, tomato aspermy virus, tomato bushy stunt virus, tomato black ring virus, tomato ringspot virus, tomato mosaic virus, pepino mosaic virus and ToBRFV using Enzyme linked immunosorbent assay (ELISA) test (LOEWE®, Biochemica, Germany), according to the manufacturers' instructions. The obtained results showed that 84.4% (38/45) of symptomatic tomato samples were infected with at least one of the detected viruses. The obtained results showed that 55.5% (25/45) of symptomatic tomato samples were found positive to ToBRFV, three out of 25 samples (12%) were singly infected, however 22 out of 45 (48.8%) had mixed infection between ToBRFV and with at least one of tested viruses. A sample with a single infection of ToBRFV was mechanically inoculated into different host range including: Chenopodium amaranticolor, C. quinoa, C. album, C. glaucum, Nicotiana glutinosa, N. benthamiana, N. tabacum, N. occidentalis, Gomphrena globosa, Datura stramonium, Solanum lycopersicum, S. nigrum, petunia hybrida and symptoms were observed weekly and the systemic presence of the ToBRFV was confirmed by RT-PCR and partial nucleotide sequence. A Total RNA was extracted from DAS-ELISA positive samples using Thermo Scientific GeneJET Plant RNA Purification Mini Kit. Reverse transcription-Polymerase chain reaction (RT-PCR) was carried out using specific primers F-3666 (5´-ATGGTACGAACGGCGGCAG-3´) and R-4718 (5´-CAATCCTTGATGTG TTTAGCAC-3´) which amplified a fragment of 1052 bp of Open Reading Frame (ORF) encoding the RNA-dependent RNA polymerase (RdRp). (Luria et al. 2017). RT-PCR products were analyzed using 1.5 % agarose gel electrophoresis. RT-PCR products were sequenced in both directions by Macrogen Inc. Seoul, South Korea. Partial nucleotide sequences obtained from selected samples were submitted to GenBank and assigned the following accession numbers: MZ130501, MZ130502, and MZ130503. BLAST analysis of Saudi isolates of ToBRFV showed that the sequence shared nucleotide identities ranged between 98.99 % to 99.50 % among them and 98.87-99.87 % identity with ToBRFV isolates from Palestine (MK881101 and MN013187), Turkey (MK888980, MT118666, MN065184, and MT107885), United Kingdom (MN182533), Egypt (MN882030 and MN882031), Jordan (KT383474), USA (MT002973), Mexico (MK273183 and MK273190), Canada (MN549395) and Netherlands (MN882017, MN882018, MN882042, MN882023, MN882024, and MN882045). To our knowledge, this is the first report of occurrence of ToBRFV infecting tomato in Saudi Arabia which suggests its likely introduction by commercial seeds from countries reported this virus and spread in greenhouses through mechanical means. The author(s) declare no conflict of interest. Keywords: Tomato brown rugose fruit virus, tomato, ELISA, RT-PCR, Saudi Arabia References: Luria N, et al., 2017. PLoS ONE 12(1): 1-19. Salem N, et al., 2015. Archives of Virology 161(2): 503-506. Fig. 1. Symptoms caused by ToBRFV showing irregular brown spots, deformation, yellowing spots on fruits (A, B, C) and bubbling and mottling, mosaic with dark green wrinkled and narrowing on leaf (D).

INDEXING TISSUE CULTURE BANANA (MUSA SPP.) SEEDLINGS FOR BANANA STREAK VIRUS WITH THE AIM OF ESTABLISHING A CLEAN MOTHER BLOCK

2010 ◽  
pp. 357-360
Author(s):  
T. Mwangi ◽  
M. Otipa ◽  
A. Wangai ◽  
L. Karanja ◽  
E. Nyaboga ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Streak Virus ◽  
Banana Streak Virus ◽  
Musa Spp

scholarly journals Primer Reporte del Virus del Rayado del Banano (BSV) Afectando Plantaciones de Plátano (Musa AAB Simmonds), Caña de azúcar (Sacharum officinarum) y Achira (Canna edulis) en Colombia

1996 ◽  
Vol 1 (1) ◽  
pp. 35 ◽  
Author(s):  
Helena Reichel ◽  
Silvio Belalcázar ◽  
Gladys Múnera ◽  
Emilio Arévalo ◽  
Javier Narváez
Keyword(s):  
Saccharum Officinarum ◽  
Streak Virus ◽  
Banana Streak Virus ◽  
First Report ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Polyclonal Antisera ◽  
Foliar Tissue ◽  
First Time ◽  
Das Elisa

<p>En noviembre de 1995, en los Municipios de Andes, Venecia e Hispania (Antioquia), La Tebaida y Montenegro (Quindío), se observaron hojas de plantas del don Dominico-Hartón (Musa AAB Simmonds) con rayas cloróticas y necróticas, síntomas que caracterizan la enfermedad del rayado del banano. En ocasiones las plantas presentaban síntomas de mosaico en el pseudotallo y el engrosamiento y/o rompimiento de la base del mismo. Además, en las cercanías de las plantaciones de plátano de los Municipios de Andes y Montenegro, se observaron respectivamente plantas de caña de azúcar (Saccharum officinarum) con síntomas de clorosis y de achira (Canna edulis) con síntomas de mosaico leve en sus hojas. Muestras de tejido foliar de éstas tres especies de plantas fueron analizadas para detectar la presencia del badnavirus del rayado del banano (BSV) y del virus del mosaico del pepino (CMV), mediante la prueba serológica DAS-ELISA, empleando anticuerpos policlonales comerciales (AGDIA Inc., Elkhart, IN). En el caso del plátano, en la región de Antioquia únicamente se detectó el BSV en el so% de las plantas sintomáticas analizadas, mientras que en el Quindío, el 6oo/o de las plantas estuvieron infectadas simultáneamente por BSV y CMV. El BSV se detectó también en muestras de tejido foliar de caña de azúcar y achira, pero en ningún caso resultaron positivas para el CMV, según la prueba DAS-ELISA. El análisis mediante microscopía electrónica de immunoabsorbancia (ISEM) del tejido foliar de plátano y caña de azúcar infectado, indicó la presencia de partículas baciliformes típicas del BSV de aproximadamente 30 x 110 nm. Hasta donde conocemos, este es el primer reporte sobre la presencia del BSV afectando plátano, caña de azúcar y achira en Colombia y es la primera vez que se reporta a la achira como hospedero de éste virus en el mundo.</p><p><strong><br /></strong></p><p><strong>First Report of Banana Streak Virus (BSV) Infecting Plantain (Musa AAB Simmonds), Sugar cane (Sacharumofficinarum) and Achira (Canna edulis) in Colombia</strong></p><p>Viruslike symptoms of yellow striate mosaic to necrotic streaks were observed on plantain leaves of the cultivar Dominico-Hartón (Musa AAB Simmonds) in the municipalities of Andes, Venecia and Hispania (Antioquia) and Tebaida and Montenegro (Quindío), Colombia. Symptoms sometimes included mosaic and swelling at the base of the pseudostem. Furthermore, in the neighborhood of the plantain crops, at the localities of Andes and Montenegro, it was observed respectively plants of sugarcane (Saccharum officinarum L.) with chlorotic foliar tissue and of achira (Canna edulis L.) with symptoms of mild mosaic in their leaves. The foliar tissue of symptomatic plants of these three species, was tested for banana streak virus (BSV ) and cucumber mosaic virus (CMV ) by double antibody sandwich enzime-linked immunosorbent assay (DAS-ELISA) with commercial polyclonal antisera (Agdia Inc., Elkhart, IN). BSV was detected in samples of all plant species. In Plantain, so% of the examined plants from the Antioquia region were infected by BSV, but not CMV; whereas, at the Quindío region, 6o% of the plants were simultaneously infected by both viruses, as detected by DAS- ELISA. CMV was not detected in foliar tissue of either sugarcane or achira plants. Immunosorbent electron microscopy analysis (ISEM) of BSV infected foliar tissue of plantain and sugarcane, showed the presence of viral bacilliform particles measuring ca. 30 x 110 nm, typical of BSV. Up to our knowledge, this is the first report of BSV infecting Musa spp., Saccharum officinarum and Canna edulis in Colombia, and the first time that Canna edulis is reported as a host for this virus.</p>

scholarly journals First Report of Banana bract mosaic virus in ‘Cavendish’ Banana in Ecuador

Plant Disease ◽  
2013 ◽  
Vol 97 (7) ◽  
pp. 1003-1003
Author(s):  
D. F. Quito-Avila ◽  
M. A. Ibarra ◽  
R. A. Alvarez ◽  
M. F. Ratti ◽  
L. Espinoza ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Genetic Relationships ◽  
The Philippines ◽  
Streak Virus ◽  
Rt Pcr ◽  
First Report ◽  
Agricultural Income ◽  
Cavendish Banana ◽  
Pcr Products ◽  
Banana Bract Mosaic Virus

Banana bract mosaic virus (BBrMV), a member of the genus Potyvirus, family Potyviridae, is the causal agent of bract mosaic disease. The disorder has been considered a serious constraint to banana and plantain production in India and the Philippines, where the virus was first identified (3). To date, the presence of BBrMV has been reported only in a few banana-growing countries in Asia (3). In the Americas, BBrMV has been detected by ELISA tests in Colombia only (1). The efficient spread of BBrMV through aphids and vegetative material increases the quarantine risk and requires strict measures to prevent entrance of the virus to new areas. In Ecuador—the world's number one banana exporter—the banana industry represents the main agricultural income source. Thus, early detection of banana pathogens is a priority. In June of 2012, mosaic symptoms in bracts and bunch distortion of ‘Cavendish’ banana were observed in a commercial field in the province of Guayas, Ecuador. Leaves from 35 symptomatic plants were tested for Cucumber mosaic virus (CMV), Banana streak virus (BSV), and BBrMV using double antibody sandwich ELISA kits from Adgen (Scotland, UK). Twenty-one plants tested positive for BBrMV but not for CMV or BSV. In order to confirm the ELISA results, fresh or lyophilized leaf extracts were used for immunocapture reverse transcription (IC-RT)-PCR. In addition, total RNA was extracted from the ELISA-positive samples and subjected to RT-PCR. The RT reactions were done using both random and oligo dT primers. Several sets of primers, flanking conserved regions of the virus coat protein (CP), have been used for PCR-detection of BBrMV (2,3,4). The Ecuadorian BBrMV isolate was successfully detected by three primer sets with reported amplification products of 324, 280, and 260 nucleotides long, respectively (3,4). Amplification products of the expected size were purified and sequenced. All the nucleotide sequences obtained from 20 PCR-positive symptomatic plants were 100% identical between each other. However, 99% identity was observed when PCR products from the Ecuadorian isolate were compared with the corresponding fragment of a BBrMV isolate from the Philippines (NCBI Accession No. DQ851496.1). PCR products of the Ecuadorian isolate, amplified by the different CP primers described above, were assembled into a 408-bp fragment and deposited in the NCBI GenBank (KC247746). Further testing confirmed the presence of BBrMV in symptomatic plants from four different provinces. To our knowledge, this is the first report of BBrMV in Ecuador and the first BBrMV partial nucleotide sequence reported from the Americas. It is worth mentioning that primer set Bract 1/Bract 2, which amplifies a 604-bp product (2), was not effective in detecting the Ecuadorian isolate. It is hypothesized that nucleotide variation at the reverse primer site is the cause of the lack of amplification with this primer set, since the forward primer is part of the sequenced product and no variation was found. Sequencing of the entire CP region is underway to conduct phylogenetic analysis and determine genetic relationships across several other BBrMV isolates. References: (1) J. J. Alarcon et al. Agron 14:65, 2006. (2) M. F. Bateson and J. L. Dale. Arch. Virol 140:515, 1995. (3) E. M. Dassanayake. Ann. Sri Lanka Dept. Agric. 3:19, 2001. (4) M. L. Iskra-Caruana et al. J. Virol. Methods 153:223, 2008.

scholarly journals First Report of Tomato spotted wilt virus in Common Agapanthus

Plant Disease ◽  
2000 ◽  
Vol 84 (4) ◽  
pp. 491-491 ◽  
Author(s):  
C. R. Wilson ◽  
A. J. Wilson ◽  
S. J. Pethybridge
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Protein Gene ◽  
Enzyme Linked Immunosorbent Assay ◽  
First Report ◽  
Tomato Spotted Wilt ◽  
Nucleocapsid Protein Gene ◽  
Polymerase Chain ◽  
Wilt Virus ◽  
Line Patterns ◽  
Leaf Symptoms

Common agapanthus (Agapanthus praecox subsp. orientalis), native to South Africa, is a popular ornamental flowering bulb species belonging to the Amaryllidaceae and is commonly found in residential gardens. Roots from some Agapanthus sp. also are used in traditional medicine in Africa. Common agapanthus collected from a residential property in Hobart, Tasmania, Australia, showed leaf symptoms of concentric ring and line patterns, irregular chlorotic blotches, and streaks. Symptomatic plants were severely stunted and failed to flower. Symptomatic leaves prematurely senesced, but young foliage subsequently produced was symptomless. Similar symptoms have been reported in other members of the Amaryllidaceae and are associated with infection by Tomato spotted wilt virus (TSWV; e.g., Nerine and Hippeastrum spp.) or Cucumber mosaic virus (CMV; e.g., Hippeastrum sp.) (2). The presence of TSWV and absence of CMV in symptomatic plants of common agapanthus was determined by enzyme-linked immunosorbent assay. Confirmation of TSWV infection was provided by reverse-transcription polymerase chain reaction assay with primers specific to the nucleocapsid protein gene of TSWV, with nucleic extracts from symptomatic plants producing an expected ≈800-bp amplicon (1). This is the first report of TSWV infection of any species within the Amaryllidaceae in Australia and the first report of the occurrence of TSWV in common agapanthus. References: (1) R. K. Jain et al. Plant Dis. 82:900, 1998. (2) G. Loebenstein et al. 1995. Virus and Virus-like Diseases of Bulb and Flower Crops. John Wiley & Sons, Chichester, U.K.

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