scholarly journals Detection of Tobacco streak virus isolates in North American Cranberry (Vaccinium macrocarpon Ait.)

2001 ◽  
Vol 2 (1) ◽  
pp. 5 ◽  
Author(s):  
A. Teifion Jones ◽  
Wendy J. McGavin ◽  
Alison Dolan

In tests on nine cranberry cultivars imported into Scotland from the USA, two cultivars, Pilgrim and AJ, were found infected with Tobacco streak virus (TSV). Three distinct isolates of this virus were detected in this infected material and were distinguished by their infection of, and reactions in, different herbaceous test plants. This is the first report of natural infection with TSV in Vaccinium species and the first characterised virus reported in cranberry. As TSV is seed and pollen transmitted in many hosts, care should be taken to test plants before propagation or when using as parents in plant breeding programs. Accepted for publication 9 July 2001. Published 17 July 2001.

2021 ◽  
Author(s):  
Ioannis E. Tzanetakis

Abstract TSV is prevalent in south-eastern Brazil, and can limit yields of soyabeans in some years (Costa and Carvalho, 1961). Epidemics of soyabean bud blight, caused by TSV, were monitored in two soyabean cultivars sown on seven different dates in 1987-88 in Arapoti County, Parana. The highest final level of disease incidence was 60% in 1987 and 90% in 1988 (Almeida et al., 1994a). Brazilian bud blight is also a serious disease in the Santa Fe province of Argentina (Laguna et al., 1988). It occurs in the USA (Fagbenle and Ford, 1970; Kaiser et al., 1982; Sherwood and Jackson, 1985) but is of no economic significance on soyabeans because conditions are not conducive to the development of the problem. TSV presents a major problem in sunflower production in the Indian subcontinent as severe necrosis symptoms may lead to major losses (Bhat et al., 2002).


Author(s):  
Amber Bassett ◽  
Kelvin Kamfwa ◽  
Daniel Ambachew ◽  
Karen Cichy

Abstract Key message Cooked bean flavor and texture vary within and across 20 Andean seed types; SNPs are significantly associated with total flavor, beany, earthy, starchy, bitter, seed-coat perception, and cotyledon texture. Abstract Common dry beans are a nutritious food recognized as a staple globally, but their consumption is low in the USA. Improving bean flavor and texture through breeding has the potential to improve consumer acceptance and suitability for new end-use products. Little is known about genetic variability and inheritance of bean sensory characteristics. A total of 430 genotypes of the Andean Diversity Panel representing twenty seed types were grown in three locations, and cooked seeds were evaluated by a trained sensory panel for flavor and texture attribute intensities, including total flavor, beany, vegetative, earthy, starchy, sweet, bitter, seed-coat perception, and cotyledon texture. Extensive variation in sensory attributes was found across and within seed types. A set of genotypes was identified that exhibit extreme attribute intensities generally stable across all three environments. seed-coat perception and total flavor intensity had the highest broad-sense heritability (0.39 and 0.38, respectively), while earthy and vegetative intensities exhibited the lowest (0.14 and 0.15, respectively). Starchy and sweet flavors were positively correlated and highest in white bean genotypes according to principal component analysis. SNPs associated with total flavor intensity (six SNPs across three chromosomes), beany (five SNPs across four chromosomes), earthy (three SNPs across two chromosomes), starchy (one SNP), bitter (one SNP), seed-coat perception (three SNPs across two chromosomes), and cotyledon texture (two SNPs across two chromosomes) were detected. These findings lay a foundation for incorporating flavor and texture in breeding programs for the development of new varieties that entice growers, consumers, and product developers alike.


Author(s):  
K. Saratbabu ◽  
K. Vemana ◽  
A.K. Patibanda ◽  
B. Sreekanth ◽  
V. Srinivasa Rao

Background: Peanut stem necrosis disease (PSND) caused by Tobacco streak virus (TSV) is a major constraint for groundnut production in Andhra Pradesh (A.P.). However, studies on prevalence and spread of the disease confined to only few districts of A.P. with this background current study focused on incidence and spread of the disease in entire state of A.P. Further an isolate of TSV occurring in A.P. characterized on the basis of genetic features by comparing with other TSV isolates originated from different hosts and locations from world.Methods: Roving survey was conducted during kharif 2017-18 in groundnut growing districts of Andhra Pradesh (A.P.) for peanut stem necrosis disease incidence. Groundnut plants showing PSND symptoms were collected and tested with direct antigen coating enzyme linked immunosorbent assay (DAC-ELISA). Groundnut samples found positive by ELISA once again tested by reverse transcription polymerase chain reaction (RT-PCR). The representative TSV-GN-INDVP groundnut isolate from Prakasham district was maintained on cowpea seedlings by standard sap inoculation method in glasshouse for further molecular characterization. The Phylogenetic tree for coat protein (CP) gene was constructed using aligned sequences with 1000 bootstrap replicates following neighbor-joining phylogeny.Result: Thirty-eight (52.7%) of seventy-two groundnut samples collected from different locations in A.P were given positive reaction to TSV by DAC-ELISA. For the first time, PSND incidence observed in coastal districts (Krishna, Guntur, Sri Pottisriramulu Nellore, Prakasham) of A.P. Maximum PSND incidence recorded from Bathalapalli (22.2%) and the minimum incidence in Mulakalacheruvu (4.1%). The coat protein (CP) gene of TSV-GN-INDVP groundnut isolate was amplified by RT-PCR and it shared maximum per cent nucleotide identity (97.51-98.62%) with TSV isolates from groundnut and other different crops reported in India. All Indian isolates cluster together irrespective of crop and location based on the phylogenetic analysis.


2003 ◽  
Vol 142 (3) ◽  
pp. 365-368 ◽  
Author(s):  
R D V J PRASADA RAO ◽  
A S REDDY ◽  
S V REDDY ◽  
K THIRUMALA-DEVI ◽  
S CHANDER RAO ◽  
...  

2005 ◽  
Vol 130 (5) ◽  
pp. 711-715 ◽  
Author(s):  
Nicholi Vorsa ◽  
James J. Polashock

The flavonoids of american cranberry (Vaccinium macrocarpon Ait.) are documented to be beneficial for human health. Among their benefits is a high antioxidant potential, with anthocyanin glycosides being the main contributors. Flavonoid glucose conjugates are reported to be more bioavailable than those with other sugar conjugates. The anthocyanin glycosides of V. macrocarpon fruit are mainly galactosides and arabinosides of the aglycones, cyanidin and peonidin, with less than 8% glucosides. In contrast, the fruit anthocyanins of another cranberry species, V. oxycoccus L. were found to be largely glucosides of cyanidin and peonidin. Interspecific hybrids between these two species were intermediate to the parental species in the proportion of fruit anthocyanin glucosides. About half the progeny (1:1 segregation) in a backcross population (to V. macrocarpon) maintained the relatively high anthocyanin glucoside ratio. In this study, we demonstrate the genetic manipulation of anthocyanin glycosylation in cranberry using interspecific hybridization, resulting in dramatically increased glucose-conjugated anthocyanins.


1969 ◽  
Vol 8 (11) ◽  
pp. 2219-2222 ◽  
Author(s):  
Rodney Croteau ◽  
Irving S. Fagerson

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