Alteration of Anthocyanin Glycosylation in Cranberry Through Interspecific Hybridization

The flavonoids of american cranberry (Vaccinium macrocarpon Ait.) are documented to be beneficial for human health. Among their benefits is a high antioxidant potential, with anthocyanin glycosides being the main contributors. Flavonoid glucose conjugates are reported to be more bioavailable than those with other sugar conjugates. The anthocyanin glycosides of V. macrocarpon fruit are mainly galactosides and arabinosides of the aglycones, cyanidin and peonidin, with less than 8% glucosides. In contrast, the fruit anthocyanins of another cranberry species, V. oxycoccus L. were found to be largely glucosides of cyanidin and peonidin. Interspecific hybrids between these two species were intermediate to the parental species in the proportion of fruit anthocyanin glucosides. About half the progeny (1:1 segregation) in a backcross population (to V. macrocarpon) maintained the relatively high anthocyanin glucoside ratio. In this study, we demonstrate the genetic manipulation of anthocyanin glycosylation in cranberry using interspecific hybridization, resulting in dramatically increased glucose-conjugated anthocyanins.

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Production and Molecular Identification of Interspecific Hybrids between Phaius mishmensis (Lindl. and Paxton) Rchb. f. and Phaius tankervilliae (Banks) Blume

Agriculture ◽

10.3390/agriculture11040306 ◽

2021 ◽

Vol 11 (4) ◽

pp. 306

Author(s):

Tasanai Punjansing ◽

Maliwan Nakkuntod ◽

Somjit Homchan ◽

Phithak Inthima ◽

Anupan Kongbangkerd

Keyword(s):

Interspecific Hybridization ◽

Interspecific Hybrids ◽

Parental Species ◽

Female Parent ◽

Genetic Fidelity ◽

Germination Percentage ◽

F1 Hybrids ◽

Scar Markers ◽

Sequence Characterized Amplified Region ◽

Decamer Primer

This study aimed at assessing the hybridization feasibility and evaluating genetic fidelity of the hybrid seedlings originated from Phaius mishmensis (Lindl. and Paxton) Rchb. f. and P. tankervilliae (Banks) Blume. Intra- and interspecific hybridization between Phaius mishmensis (Lindl. and Paxton) Rchb. f. and P. tankervilliae (Banks) Blume were examined to establish the primary hybrid, observe their cross ability and identify the F1 hybrids using sequence-characterized amplified region (SCAR) markers. Self-incompatibility and cross ability of P. mishmensis and P. tankervilliae were tested before starting the breeding program. Results showed that they were self-compatible orchids. The interspecific hybridization between P. mishmensis and P. tankervilliae was achieved with the highest pod setting (80%), seed germination percentage (94.8%) and the rate of protocorm development into mature seedlings (stage 6) (10.6%), but the smallest size of embryo with width 46.5 μm, length 67.3 μm was also observed when P. mishmensis was taken as the female parent. A comparative study on leaf morphology and anatomy of plantlets regenerated from intra- and interspecific hybrids of P. mishmensis and P. tankervilliae showed a transitional character to the parental species. Herein, the presence of interspecific hybrids between P. mishmensis and P. tankervilliae, as well as their reciprocal cross, was verified using Pmis524 SCAR markers developed by the decamer primer.

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Seed lipids of the American cranberry (vaccinium macrocarpon)

Phytochemistry ◽

10.1016/s0031-9422(00)88185-6 ◽

1969 ◽

Vol 8 (11) ◽

pp. 2219-2222 ◽

Cited By ~ 9

Author(s):

Rodney Croteau ◽

Irving S. Fagerson

Keyword(s):

Vaccinium Macrocarpon ◽

Seed Lipids ◽

American Cranberry

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Interspecific hybridization can generate functional novelty in cichlid fish

Proceedings of The Royal Society B Biological Sciences ◽

10.1098/rspb.2019.1621 ◽

2019 ◽

Vol 286 (1913) ◽

pp. 20191621 ◽

Cited By ~ 6

Author(s):

O. M. Selz ◽

O. Seehausen

Keyword(s):

Interspecific Hybridization ◽

First Generation ◽

Parental Species ◽

Cichlid Fish ◽

Context Dependency ◽

Functional Variation ◽

Generate Functional ◽

Cichlid Species ◽

Evolutionary Consequences ◽

African Cichlids

The role of interspecific hybridization in evolution is still being debated. Interspecific hybridization has been suggested to facilitate the evolution of ecological novelty, and hence the invasion of new niches and adaptive radiation when ecological opportunity is present beyond the parental species niches. On the other hand, hybrids between two ecologically divergent species may perform less well than parental species in their respective niches because hybrids would be intermediate in performance in both niches. The evolutionary consequences of hybridization may hence be context-dependent, depending on whether ecological opportunities, beyond those of the parental species, do or do not exist. Surprisingly, these complementary predictions may never have been tested in the same experiment in animals. To do so, we investigate if hybrids between ecologically distinct cichlid species perform less well than the parental species when feeding on food either parent is adapted to, and if the same hybrids perform better than their parents when feeding on food none of the species are adapted to. We generated two first-generation hybrid crosses between species of African cichlids. In feeding efficiency experiments we measured the performance of hybrids and parental species on food types representing both parental species niches and additional ‘novel’ niches, not used by either of the parental species but by other species in the African cichlid radiations. We found that hybrids can have higher feeding efficiencies on the ‘novel’ food types but typically have lower efficiencies on parental food types when compared to parental species. This suggests that hybridization can generate functional variation that can be of ecological relevance allowing the access to resources outside of either parental species niche. Hence, we provide support for the hypothesis of ecological context-dependency of the evolutionary impact of interspecific hybridization.

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Antibacterial activities of a polyphenolic-rich extract prepared from American cranberry (Vaccinium macrocarpon) fruit pomace against Listeria spp.

LWT ◽

10.1016/j.lwt.2020.109056 ◽

2020 ◽

Vol 123 ◽

pp. 109056 ◽

Cited By ~ 4

Author(s):

Moussa S. Diarra ◽

Yousef I. Hassan ◽

Glenn S. Block ◽

John C.G. Drover ◽

Pascal Delaquis ◽

...

Keyword(s):

Antibacterial Activities ◽

Vaccinium Macrocarpon ◽

American Cranberry ◽

Fruit Pomace

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Variability in the Qualitative and Quantitative Composition and Content of Phenolic Compounds in the Fruit of Introduced American Cranberry (Vaccinium macrocarpon Aiton)

Plants ◽

10.3390/plants9101379 ◽

2020 ◽

Vol 9 (10) ◽

pp. 1379

Author(s):

Ieva Gudžinskaitė ◽

Elicija Stackevičienė ◽

Mindaugas Liaudanskas ◽

Kristina Zymonė ◽

Vaidotas Žvikas ◽

...

Keyword(s):

Phenolic Compounds ◽

Vaccinium Macrocarpon ◽

Quantitative Composition ◽

Qualitative And Quantitative ◽

American Cranberry ◽

Fruit Samples ◽

Phytochemical Profile ◽

Ethanol Extracts ◽

The Individual

The aim of this study was to determine the composition and content of phenolic compounds in ethanol extracts of eight different cultivars of American cranberry (Vaccinium macrocarpon Aiton) fruit using spectrophotometric and UPLC-ESI-MS/MS analysis and to evaluate the antioxidant activity in vitro of these extracts. The highest total amount of phenolic compounds evaluated via Folin–Ciocalteu spectrophotometry was detected in American cranberry fruit samples of the ‘Bain’ clone, and the highest total amount of flavonoids was found in samples of the ‘Drever’ and ‘Baiwfay’ cultivars. The highest total amount of the individual phenolic compounds (519.53 ± 25.12 mg/g DW) identified and quantitatively evaluated via chromatography was detected in samples of the ‘Searles’ cranberry cultivar. In the studied cranberry samples, the predominant phenolic compounds were hyperoside, quercetin, and procyanidin A2, while the amounts of other compounds were significantly lower. HCA and PCA revealed that ‘Woolman’, ‘Holliston’, ‘Pilgrim, and ‘Searles’ fruit samples had different quantitative content of phenolic compounds from other cranberry cultivars. Meanwhile, fruit of ‘Baiwfay’, ‘Drever’, ‘Bain’, and ‘Bergman’ were similar in their phytochemical profile.

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Interspecific hybridization of sturgeon species affects differently their gonadal development

Czech Journal of Animal Science ◽

10.17221/37/2016-cjas ◽

2017 ◽

Vol 63 (No. 1) ◽

pp. 1-10 ◽

Cited By ~ 7

Author(s):

Z. Linhartová ◽

M. Havelka ◽

M. Pšenička ◽

M. Flajšhans

Keyword(s):

Interspecific Hybridization ◽

Germ Cells ◽

Sertoli Cells ◽

Interspecific Hybrids ◽

Gonad Development ◽

Gonadal Development ◽

Genetic Origin ◽

Ploidy Levels ◽

Sturgeon Species ◽

Fertile Hybrids

Gonad development in fish is generally assumed to be negatively influenced by interspecific hybridization, resulting in sterility or sub-sterility. However, this is not the case in sturgeons (Acipenseridae), in which fertile hybrids are common. In the present study, we investigated gonad development in several sturgeon interspecific hybrids and purebred species. Six interspecific hybrid groups and three purebred groups were analyzed including 20 hybrid specimens with even ploidy, 40 specimens having odd ploidy levels, and 30 purebred specimens. Hybrids of species with the same ploidy (even ploidy – 2n, 4n) exhibited normally developed gonads similar to those seen in purebred specimens. In contrast, hybrids of species differing in ploidy (odd ploidy – 3n) did not display fully developed gonads. Ovaries were composed of oocytes or nests of differentiating oocytes that ceased development in early stages of meiosis (pachytene to zygotene) with a higher content of adipose and apoptotic tissue. Testes contained single spermatogonia along with Sertoli cells and spaces lacking germ cells. The obtained results showed that gonad development was influenced by genetic origin and ploidy of the sturgeon hybrids and were consistent with full fertility of hybrids with even ploidy. Sterility of females, but possibly limited fertility of males, is suggested for hybrids with odd ploidy.

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Oligo-painting and GISH reveal meiotic chromosome biases and increased meiotic stability in synthetic allotetraploid Cucumis ×hytivus with dysploid parental karyotypes

BMC Plant Biology ◽

10.1186/s12870-019-2060-z ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 1

Author(s):

Qinzheng Zhao ◽

Yunzhu Wang ◽

Yunfei Bi ◽

Yufei Zhai ◽

Xiaqing Yu ◽

...

Keyword(s):

Interspecific Hybridization ◽

Interspecific Hybrids ◽

Chromosome Doubling ◽

Meiotic Chromosome ◽

Genomic In Situ Hybridization ◽

Meiotic Behavior ◽

Cytological Evidence ◽

Meiotic Stability ◽

Inbred Family

Abstract Background Meiosis of newly formed allopolyploids frequently encounter perturbations induced by the merging of divergent and hybridizable genomes. However, to date, the meiotic properties of allopolyploids with dysploid parental karyotypes have not been studied in detail. The allotetraploid Cucumis ×hytivus (HHCC, 2n = 38) was obtained from interspecific hybridization between C. sativus (CC, 2n = 14) and C. hystrix (HH, 2n = 24) followed by chromosome doubling. The results of this study thus offer an excellent opportunity to explore the meiotic properties of allopolyploids with dysploid parental karyotypes. Results In this report, we describe the meiotic properties of five chromosomes (C5, C7, H1, H9 and H10) and two genomes in interspecific hybrids and C. ×hytivus (the 4th and 14th inbred family) through oligo-painting and genomic in situ hybridization (GISH). We show that 1) only two translocations carrying C5-oligo signals were detected on the chromosomes C2 and C4 of one 14th individual by the karyotyping of eight 4th and 36 14th plants based on C5- and C7-oligo painting, and possible cytological evidence was observed in meiosis of the 4th generation; 2) individual chromosome have biases for homoeologous pairing and univalent formation in F1 hybrids and allotetraploids; 3) extensive H-chromosome autosyndetic pairings (e.g., H-H, 25.5% PMCs) were observed in interspecific F1 hybrid, whereas no C-chromosome autosyndetic pairings were observed (e.g. C-C); 4) the meiotic properties of two subgenomes have significant biases in allotetraploids: H-subgenome exhibits higher univalent and chromosome lagging frequencies than C-subgenome; and 5) increased meiotic stability in the S14 generation compared with the S4 generation, including synchronous meiosis behavior, reduced incidents of univalent and chromosome lagging. Conclusions These results suggest that the meiotic behavior of two subgenomes has dramatic biases in response to interspecific hybridization and allopolyploidization, and the meiotic behavior harmony of subgenomes is a key subject of meiosis evolution in C. ×hytivus. This study helps to elucidate the meiotic properties and evolution of nascent allopolyploids with the dysploid parental karyotypes.

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