Development and characterization of novel wheat-rye 1RS•1BL translocation lines with high resistance to Puccinia striiformis f. sp. tritici

Wheat-rye 1RS•1BL translocations from Petkus rye have contributed substantially to wheat production worldwide with their great disease resistance and yield traits. However, the resistance genes on the 1RS chromosomes have completely lost their resistance to newly emerged pathogens. Rye could widen the variation of 1RS as a naturally cross-pollinated related species of wheat. In this study, we developed three new 1RS•1BL translocation lines by crossing rye inbred line BL1, selected from Chinese landrace rye Baili, with wheat cultivar Mianyang11. These three new translocation lines exhibited high resistance to the most virulent and frequently occurring stripe rust pathotypes and showed high resistance in the field where stripe rust outbreaks have been most severe in China. One new gene for stripe rust resistance, located on 1RS of the new translocation lines, is tentatively named YrRt1054. YrRt1054 confers resistance to Puccinia striiformis f. sp. tritici pathotypes that are virulent toward Yr9 and YrCn17. This new resistance gene, YrRt1054, is available for wheat improvement programs. The present study indicated that rye cultivars may carry additional untapped variation as potential sources of resistance.

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Molecular cytogenetic characterization of wheat–Elymus repens chromosomal translocation lines with resistance to Fusarium head blight and stripe rust

BMC Plant Biology ◽

10.1186/s12870-019-2208-x ◽

2019 ◽

Vol 19 (1) ◽

Author(s):

Biran Gong ◽

Wei Zhu ◽

Sanyue Li ◽

Yuqi Wang ◽

Lili Xu ◽

...

Keyword(s):

Fusarium Head Blight ◽

Stripe Rust ◽

Puccinia Striiformis ◽

Chromosomal Translocation ◽

Stripe Rust Resistance ◽

Translocation Line ◽

Head Blight ◽

Elymus Repens ◽

Translocation Lines

Abstract Background Fusarium head blight (FHB) caused by the fungus Fusarium graminearum Schwabe and stripe rust caused by Puccinia striiformis f. sp. tritici are devastating diseases that affect wheat production worldwide. The use of disease-resistant genes and cultivars is the most effective means of reducing fungicide applications to combat these diseases. Elymus repens (2n = 6x = 42, StStStStHH) is a potentially useful germplasm of FHB and stripe rust resistance for wheat improvement. Results Here, we report the development and characterization of two wheat–E. repens lines derived from the progeny of common wheat–E. repens hybrids. Cytological studies indicated that the mean chromosome configuration of K15–1192-2 and K15–1194-2 at meiosis were 2n = 42 = 0.86 I + 17.46 II (ring) + 3.11 II (rod) and 2n = 42 = 2.45 I + 14.17 II (ring) + 5.50 II (rod) + 0.07 III, respectively. Genomic and fluorescence in situ hybridization karyotyping and simple sequence repeats markers revealed that K15–1192-2 was a wheat–E. repens 3D/?St double terminal chromosomal translocation line. Line K15–1194-2 was identified as harboring a pair of 7DS/?StL Robertsonian translocations and one 3D/?St double terminal translocational chromosome. Further analyses using specific expressed sequence tag-SSR markers confirmed that the wheat–E. repens translocations involved the 3St chromatin in both lines. Furthermore, compared with the wheat parent Chuannong16, K15–1192-2 and K15–1194-2 expressed high levels of resistance to FHB and stripe rust pathogens prevalent in China. Conclusions Thus, this study has determined that the chromosome 3St of E. repens harbors gene(s) highly resistant to FHB and stripe rust, and chromatin of 3St introgressed into wheat chromosomes completely presented the resistance, indicating the feasibility of using these translocation lines as novel material for breeding resistant wheat cultivars and alien gene mining.

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Identification, Characterization, and Evaluation of Novel Stripe Rust-Resistant Wheat–Thinopyrum intermedium Chromosome Translocation Lines

Plant Disease ◽

10.1094/pdis-01-19-0001-re ◽

2020 ◽

Vol 104 (3) ◽

pp. 875-881

Author(s):

Xingwei Zheng ◽

Caiguo Tang ◽

Ran Han ◽

Jiajia Zhao ◽

Ling Qiao ◽

...

Keyword(s):

Stripe Rust ◽

Chromosome Translocation ◽

Stripe Rust Resistance ◽

Scar Markers ◽

Thinopyrum Intermedium ◽

Wheat Improvement ◽

Improvement Programs ◽

Translocation Lines ◽

Chromosome Translocation Lines ◽

Intermedium Chromosome

Stripe rust is an important disease in wheat, and development of genetic resistance in cultivars is an effective approach to control the disease. Wild species of wheat, such as Thinopyrum intermedium, are an excellent gene source for wheat improvement. In this study, two stripe rust-resistant wheat–Th. intermedium chromosome translocation lines, CH4131 and CH4132, were characterized by cytogenetic and pathological methods. The introgressed chromosome fragment was tagged using amplified fragment-length polymorphism-derived sequence-characterized amplified region (SCAR) markers and intron targeting markers, indicating that CH4131 and CH4132 both possess a homologous group 3 chromatin of Th. intermedium. Genomic in situ hybridization results suggested that a very small Th. intermedium chromosome segment was translocated to the terminal region of wheat 1BS for both lines, forming a configuration of T3Ai-1BS.1BL. The two translocation lines were resistant to stripe rust, and the resistance gene, temporarily designated YrCH-1BS, was likely derived from Th. intermedium. The translocated chromosome fragments have no genetic linkage drag to agronomic performance. The grain quality indexes of these two translocations were higher than local wheat varieties. Therefore, CH4131 and CH4132 could be used as potential gene sources in wheat improvement programs. The SCAR markers are useful to select stripe rust resistance from Th. intermedium.

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Molecular Mapping of Stripe Rust Resistance Gene Yr81 in a Common Wheat Landrace Aus27430

Plant Disease ◽

10.1094/pdis-06-18-1055-re ◽

2019 ◽

Vol 103 (6) ◽

pp. 1166-1171 ◽

Cited By ~ 14

Author(s):

Mesfin Gessese ◽

Harbans Bariana ◽

Debbie Wong ◽

Matthew Hayden ◽

Urmil Bansal

Keyword(s):

Stripe Rust ◽

Rust Resistance ◽

Molecular Mapping ◽

Puccinia Striiformis ◽

Stripe Rust Resistance ◽

Chromosomal Assignment ◽

Resistance Locus ◽

Sources Of Resistance ◽

Stripe Rust Resistance Gene ◽

Ril Population

The deployment of diverse sources of resistance in new cultivars underpins durable control of rust diseases. Aus27430 exhibited a moderate level of stripe rust resistance against Puccinia striiformis f. sp. tritici (Pst) pathotypes currently prevalent in Australia. Aus27430 was crossed with the susceptible parent Avocet S (AvS) and subsequent filial generations were raised. Monogenic segregation observed among Aus27430/AvS F3 families was confirmed through stripe rust screening of an F6 recombinant inbred line (RIL) population, and the resistance locus was temporarily named YrAW5. Selective genotyping using an Illumina iSelect 90K wheat SNP bead chip array located YrAW5 in chromosome 6A. Genetic mapping of the RIL population with linked 90K SNPs that were converted into PCR-based marker assays, as well as SSR markers previously mapped to chromosome 6A, confirmed the chromosomal assignment for YrAW5. Comparative analysis of other stripe rust resistance genes located in chromosome 6A led to the formal designation of YrAW5 as Yr81. Tests with a marker linked with Yr18 also demonstrated the presence of this gene in Aus27430. Yr18 interacted with Yr81 to produce stripe rust responses lower than those produced by RILs carrying these genes individually. Although gwm459 showed higher recombination with Yr81 compared with the other flanking marker KASP_3077, it amplified the AvS allele in 80 cultivars, whereas KASP_3077 amplified AvS allele in 67 cultivars. Both markers can be used in marker-assisted selection after confirming parental polymorphism.

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Identification of Genomic Regions Controlling Adult-Plant Stripe Rust Resistance in Chinese Landrace Pingyuan 50 Through Bulked Segregant Analysis

Phytopathology ◽

10.1094/phyto-100-4-0313 ◽

2010 ◽

Vol 100 (4) ◽

pp. 313-318 ◽

Cited By ~ 56

Author(s):

Caixia Lan ◽

Shanshan Liang ◽

Xiangchun Zhou ◽

Gang Zhou ◽

Qinglin Lu ◽

...

Keyword(s):

Stripe Rust ◽

Puccinia Striiformis ◽

Bulked Segregant Analysis ◽

Interval Mapping ◽

Mean Value ◽

Wheat Breeding ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Phenotypic Variance ◽

Chinese Landrace

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most widespread and destructive wheat diseases worldwide. Growing resistant cultivars with adult-plant resistance (APR) is an effective approach for the control of the disease. In this study, 540 simple sequence repeat markers were screened to map quantitative trait loci (QTL) for APR to stripe rust in a doubled haploid (DH) population of 137 lines derived from the cross Pingyuan 50 × Mingxian 169. The DH lines were planted in randomized complete blocks with three replicates in Gansu and Sichuan provinces during the 2005–06, 2006–07, and 2007–08 cropping seasons, providing data for four environments. Artificial inoculations were carried out in Gansu and Sichuan with the prevalent Chinese race CYR32. Broad-sense heritability of resistance to stripe rust for maximum disease severity was 0.91, based on the mean value averaged across four environments. Inclusive composite interval mapping detected three QTL for APR to stripe rust on chromosomes 2BS, 5AL, and 6BS, designated QYr.caas-2BS, QYr.caas-5AL, and QYr.caas-6BS, respectively, separately explaining from 4.5 to 19.9% of the phenotypic variation. QYr.caas-5AL, different from QTL previously reported, was flanked by microsatellite markers Xwmc410 and Xbarc261, and accounted for 5.0 to 19.9% of phenotypic variance. Molecular markers closely linked to the QTL could be used in marker-assisted selection for APR to stripe rust in wheat breeding programs.

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Utilization of the Genomewide Wheat 55K SNP Array for Genetic Analysis of Stripe Rust Resistance in Common Wheat Line P9936

Phytopathology ◽

10.1094/phyto-10-18-0388-r ◽

2019 ◽

Vol 109 (5) ◽

pp. 819-827 ◽

Cited By ~ 6

Author(s):

Shuo Huang ◽

Jianhui Wu ◽

Xiaoting Wang ◽

Jingmei Mu ◽

Zhi Xu ◽

...

Keyword(s):

Stripe Rust ◽

Puccinia Striiformis ◽

Recombinant Inbred Lines ◽

Snp Array ◽

Snp Markers ◽

Stripe Rust Resistance ◽

Biparental Population ◽

Chinese Landrace ◽

Allele Specific ◽

Rust Severity

Breeding for resistance to stripe rust (caused by Puccinia striiformis f. tritici) is essential for reducing losses in yield and quality in wheat. To identify genes for use in breeding, a biparental population of 186 recombinant inbred lines (RILs) from a cross of the Chinese landrace Mingxian 169 and CIMMYT-derived line P9936 was evaluated in field nurseries either artificially or naturally inoculated in two crop seasons. Each of the RILs and parents was genotyped with the wheat 55K single-nucleotide polymorphism (SNP) ‘Breeders’ array and a genetic linkage map with 8,225 polymorphic SNP markers spanning 3,593.37 centimorgans was constructed. Two major quantitative trait loci (QTL) and two minor QTL were identified. The major QTL QYr.nwafu-3BS.2 and QYr.nwafu-7BL on chromosomes arms 3BS and 7BL were detected in all field locations and explained an average 20.4 and 38.9% of phenotypic variation stripe rust severity, respectively. QYr.nwafu-3BS.2 likely corresponds to the locus Yr30/Sr2 and QYr.nwafu-7BL may be a resistance allele identified previously in CIMMYT germplasm. The other minor QTL had limited individual effects but increased resistance when in combinations with other QTL. Markers linked to QYr.nwafu-7BL were converted to kompetitive allele-specific polymerase chain reaction markers and validated in a panel of wheat accessions. Wheat accessions carrying the same haplotype as P9936 at the identified SNP loci had lower average stripe rust severity than the average severity of all other haplotypes.

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Molecular mapping as a tool for pre-emptive breeding for resistance to the exotic barley pathogen, Puccinia striiformis f. sp. hordei

Australian Journal of Agricultural Research ◽

10.1071/ar02231 ◽

2003 ◽

Vol 54 (12) ◽

pp. 1351 ◽

Cited By ~ 5

Author(s):

M. Cakir ◽

M. Spackman ◽

C. R. Wellings ◽

N. W. Galwey ◽

D. B. Moody ◽

...

Keyword(s):

Stripe Rust ◽

Molecular Mapping ◽

Puccinia Striiformis ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Sources Of Resistance ◽

Plant Stage ◽

The Usa ◽

Alternative Sources ◽

Barley Stripe Rust

Barley stripe rust (BSR), caused by Puccinia striiformis f. sp. hordei, has been a major disease problem to the barley industry worldwide that has not, to date, been detected in Australia. This paper describes the mapping of stripe rust resistance genes in Tallon/Kaputar (TK) and Arapiles/Franklin (AF) populations. The paper also reports on the usefulness of markers associated with alternative sources of resistance previously identified in the varieties Orca and Shyri in the USA. Stripe rust screening was conducted at the adult plant stage in Toluca, Mexico, for 2 years. Two major quantitative trait loci (QTLs) were found on chromosomes 2H and 5H in both populations. One region on chromosome 5H was highly significantly associated with resistance to stripe rust (R2 = 68% and 34% in TK and AF, respectively). The QTL on chromosome 2H accounted for 36% of the variation in TK and 10% of the variation in the AF population. These associations were consistent over both years. Further work will involve screening for additional markers in the target regions to identify polymorphism that can be used to select for multiple resistances in the absence of the pathogen.

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Development and molecular characterization of wheat-Aegilops peregrina Introgression Lines with resistance to leaf rust and stripe rust

10.1101/160648 ◽

2017 ◽

Author(s):

Deepika Narang ◽

Satinder Kaur ◽

Jyoti Saini ◽

Parveen Chhuneja

Keyword(s):

Leaf Rust ◽

Molecular Characterization ◽

Stripe Rust ◽

Puccinia Striiformis ◽

Puccinia Triticina ◽

Introgression Lines ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Plant Stage

AbstractA wild non-progenitor species from wheat tertiary gene pool Aegilops peregrina accession pau3519 (UUSS) was used for introgression of leaf rust and stripe rust resistance in bread wheat. It was crossed and backcrossed with hexaploid wheat line Chinese Spring PhI to develop two homozygous BC2F6 wheat-Ae. peregrina introgression lines (ILs) viz. IL pau16058 and IL pau16061 through induced homoeologous recombination. Homozygous lines were screened against six Puccinia triticina and two Puccinia striiformis f. sp. tritici pathotypes at the seedling stage and a mixture of prevalent pathotypes of both rust pathogens at the adult plant stage. IL pau16061 showed resistance to leaf rust only while IL pau16058 was resistant to both leaf and stripe rust pathotypes throughout plant life. Molecular characterization of these ILs aided in defining the introgressed regions. Identification of linked markers with advance genomic technologies will aid in marker assisted pyramiding of alien genes in cultivated wheat background.

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Characterization of Wheat Stripe Rust Resistance Genes in Shaanmai 139

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2010.00109 ◽

2010 ◽

Vol 36 (1) ◽

pp. 109-114 ◽

Cited By ~ 8

Author(s):

Hong ZHANG ◽

Zhi-Long REN ◽

Yin-Gang HU ◽

Chang-You WANG ◽

Wan-Quan JI

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Wheat Stripe Rust ◽

Rust Resistance Genes

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Rapid identification of a stripe rust resistance gene YrXK in Chinese wheat line Xike01015 using specific locus amplified fragment (SLAF) sequencing

Plant Disease ◽

10.1094/pdis-12-20-2648-re ◽

2021 ◽

Author(s):

Cai Sun ◽

Yike Liu ◽

Qiang Li ◽

Baotong Wang ◽

Shuhui Chen ◽

...

Keyword(s):

Stripe Rust ◽

Puccinia Striiformis ◽

Gene Annotation ◽

Dominant Gene ◽

Rapid Identification ◽

Wheat Breeding ◽

Stripe Rust Resistance ◽

Phenotypic Variance ◽

F2 Population ◽

Chinese Wheat

Wheat stripe rust, an airborne fungal disease and caused by Puccinia striiformis Westend. f. sp. tritici (Pst), is one of the most devastating diseases on wheat. It is the most effective and economical measure for the diseases control to identify high-level resistance genes and apply in wheat breeding. Chinese wheat cultivar Xike01015 presents high levels of all stage resistance (ASR) to the current predominant Pst race CYR33. In this study, a single dominant gene, designated as YrXk, was identified in Xike01015 conferring resistance to CYR33 with genetic analysis of F2 and BC1 population from cross of Mingxian169 (susceptible) and Xike01015. The specific length amplified fragment sequencing (SLAF-seq) strategy was used to construct linkage map in the F2 population. QTL analysis mapped YrXk to a 12.4 Mb segment on chromosome1BS, explaining over 86.96% phenotypic variance. Gene annotation in the QTL region identified three differential expressed candidate genes , TraesCS1B02G168600.1, TraesCS1B02G170200.1, and TraesCS1B02G172400.1. The qRT-PCR results displayed that TraesCS1B02G170200.1 and TraesCS1B02G168600.1 significantly up-regulated and down-regulated, respectively, and TraesCS1B02G170200.1 slightly up-regulated after changed with CYR33 in the seedling stage, which indicating these genes may function in wheat resistance to stripe rust. The results of this study can be used in wheat breeding for improving resistance to stripe rust.

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Distribution of Puccinia striiformis f. sp. tritici races and virulence in wheat growing regions of Kenya from 1970 TO 2014

Plant Disease ◽

10.1094/pdis-11-20-2341-re ◽

2021 ◽

Author(s):

Mercy Wamalwa ◽

Ruth Wanyera ◽

Julian Rodriguez-Algaba ◽

Lesley Boyd ◽

James Owuoche ◽

...

Keyword(s):

Stripe Rust ◽

Fungal Pathogen ◽

Puccinia Striiformis ◽

Yellow Rust ◽

Stripe Rust Resistance ◽

Scar Markers ◽

Seedling Resistance ◽

Sequence Characterized Amplified Region ◽

Triticum Spp ◽

Virulence Diversity

Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a major threat to wheat (Triticum spp.) production worldwide. The objective of this study was to determine the virulence of Pst races prevalent in the main wheat growing regions of Kenya, which includes Mt. Kenya, Eastern Kenya, and the Rift Valley (Central, Southern, and Northern Rift). Fifty Pst isolates collected from 1970 to 1992 and from 2009 to 2014 were virulence phenotyped using stripe rust differential sets, and 45 isolates were genotyped with sequence characterized amplified region (SCAR) markers to differentiate among the isolates and identify aggressive strains PstS1 and PstS2. Virulence corresponding to stripe rust resistance genes Yr1, Yr2, Yr3, Yr6, Yr7, Yr8, Yr9, Yr17, Yr25, Yr27 and the seedling resistance in genotype Avocet S were detected. Ten races were detected in the Pst samples obtained from 1970 to 1992, and three additional races were detected from 2009 to 2014, with a single race being detected in both periods. The SCAR markers detected both Pst1 and Pst2 strains in the collection. Increasing Pst virulence was found in the Kenyan Pst population, and that diverse Pst race groups dominated different wheat growing regions. Moreover, recent Pst races in east Africa indicated possible migration of some race groups into Kenya from other regions. This study is important in understanding Pst evolution and virulence diversity and useful in breeding wheat cultivars with effective resistance to stripe rust. Keywords: pathogenicity, Puccinia f. sp. tritici stripe (yellow) rust, Triticum aestivum

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