scholarly journals Identification of Genomic Regions Controlling Adult-Plant Stripe Rust Resistance in Chinese Landrace Pingyuan 50 Through Bulked Segregant Analysis

2010 ◽  
Vol 100 (4) ◽  
pp. 313-318 ◽  
Author(s):  
Caixia Lan ◽  
Shanshan Liang ◽  
Xiangchun Zhou ◽  
Gang Zhou ◽  
Qinglin Lu ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Bulked Segregant Analysis ◽  
Interval Mapping ◽  
Mean Value ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Phenotypic Variance ◽  
Chinese Landrace

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most widespread and destructive wheat diseases worldwide. Growing resistant cultivars with adult-plant resistance (APR) is an effective approach for the control of the disease. In this study, 540 simple sequence repeat markers were screened to map quantitative trait loci (QTL) for APR to stripe rust in a doubled haploid (DH) population of 137 lines derived from the cross Pingyuan 50 × Mingxian 169. The DH lines were planted in randomized complete blocks with three replicates in Gansu and Sichuan provinces during the 2005–06, 2006–07, and 2007–08 cropping seasons, providing data for four environments. Artificial inoculations were carried out in Gansu and Sichuan with the prevalent Chinese race CYR32. Broad-sense heritability of resistance to stripe rust for maximum disease severity was 0.91, based on the mean value averaged across four environments. Inclusive composite interval mapping detected three QTL for APR to stripe rust on chromosomes 2BS, 5AL, and 6BS, designated QYr.caas-2BS, QYr.caas-5AL, and QYr.caas-6BS, respectively, separately explaining from 4.5 to 19.9% of the phenotypic variation. QYr.caas-5AL, different from QTL previously reported, was flanked by microsatellite markers Xwmc410 and Xbarc261, and accounted for 5.0 to 19.9% of phenotypic variance. Molecular markers closely linked to the QTL could be used in marker-assisted selection for APR to stripe rust in wheat breeding programs.

scholarly journals QTL mapping of adult plant resistance to stripe rust in the Fundulea 900 × Thatcher RIL population

2021 ◽  
Vol 57 (No. 1) ◽  
pp. 1-8
Author(s):  
Xiaocui Yan ◽  
Huimin Zheng ◽  
Peipei Zhang ◽  
Gebrewahid Takele Weldu ◽  
Zaifeng Li ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Recombinant Inbred Lines ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Phenotypic Variance ◽  
Wheat Stripe Rust ◽  
Ril Population

Wheat stripe rust, caused by Puccinia striiformis Westend. f.sp. tritici Eriks (Pst), is one of the most important diseases of bread wheat worldwide. Breeding resistant wheat cultivars is the most economical, effective and environmentally friendly way for controlling wheat stripe rust in China. The Romanian wheat line Fundulea 900 showed good resistance to wheat stripe rust at the adult stage. The present study aimed to map the quantitative trait loci (QTLs) for stripe rust resistance in 176 F<sub>2:6</sub> recombinant inbred lines (RIL) derived from the cross of Fundulea 900 × Thatcher. The RIL population was phenotyped for stripe rust (YR) severity at Mianyang in the Sichuan province and Baoding in the Hebei province in the 2016/2017 and 2017/2018 cropping seasons. SSR markers combined with a preferred screened group (PSG) analysis were used to identify the QTLs for stripe rust in the population. Three QTLs for stripe rust resistance were mapped on chromosomes 1AL, 7BL and 7DS, respectively. All three QTLs originated from Fundulea 900 and were detected in all the environments. The QTL on 7DS was provided by the known resistance gene Yr18/Lr34. The two QTLs on chromosomes 1AL and 7BL were explained by 9.2 to 21.5% and 5.1 to 10.1% of the phenotypic variance, respectively and might be new QTLs. The QTLs identified in the study and their closely linked markers can be used for marker-assisted selection (MAS) in wheat breeding programmes.

Rapid identification of a stripe rust resistance gene YrXK in Chinese wheat line Xike01015 using specific locus amplified fragment (SLAF) sequencing

Plant Disease ◽  
2021 ◽  
Author(s):  
Cai Sun ◽  
Yike Liu ◽  
Qiang Li ◽  
Baotong Wang ◽  
Shuhui Chen ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Gene Annotation ◽  
Dominant Gene ◽  
Rapid Identification ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Phenotypic Variance ◽  
F2 Population ◽  
Chinese Wheat

Wheat stripe rust, an airborne fungal disease and caused by Puccinia striiformis Westend. f. sp. tritici (Pst), is one of the most devastating diseases on wheat. It is the most effective and economical measure for the diseases control to identify high-level resistance genes and apply in wheat breeding. Chinese wheat cultivar Xike01015 presents high levels of all stage resistance (ASR) to the current predominant Pst race CYR33. In this study, a single dominant gene, designated as YrXk, was identified in Xike01015 conferring resistance to CYR33 with genetic analysis of F2 and BC1 population from cross of Mingxian169 (susceptible) and Xike01015. The specific length amplified fragment sequencing (SLAF-seq) strategy was used to construct linkage map in the F2 population. QTL analysis mapped YrXk to a 12.4 Mb segment on chromosome1BS, explaining over 86.96% phenotypic variance. Gene annotation in the QTL region identified three differential expressed candidate genes , TraesCS1B02G168600.1, TraesCS1B02G170200.1, and TraesCS1B02G172400.1. The qRT-PCR results displayed that TraesCS1B02G170200.1 and TraesCS1B02G168600.1 significantly up-regulated and down-regulated, respectively, and TraesCS1B02G170200.1 slightly up-regulated after changed with CYR33 in the seedling stage, which indicating these genes may function in wheat resistance to stripe rust. The results of this study can be used in wheat breeding for improving resistance to stripe rust.

scholarly journals Emerging Yr26-Virulent Races of Puccinia striiformis f. tritici Are Threatening Wheat Production in the Sichuan Basin, China

Plant Disease ◽  
2015 ◽  
Vol 99 (6) ◽  
pp. 754-760 ◽  
Author(s):  
D. J. Han ◽  
Q. L. Wang ◽  
X. M. Chen ◽  
Q. D. Zeng ◽  
J. H. Wu ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Sichuan Basin ◽  
Puccinia Striiformis ◽  
Field Tests ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Breeding Lines ◽  
Wheat Production ◽  
Stripe Rust Resistance Gene ◽  
The Sichuan Basin

Stripe rust, caused by Puccinia striiformis f. tritici, is one of the most destructive diseases of wheat in the world. The Sichuan Basin is one of the most important regions of wheat production and stripe rust epidemics in China. Stripe rust resistance gene Yr26 (the same gene as Yr24) has been widely used in wheat breeding programs and in many cultivars grown in this region since the gene was discovered in the early 1990s. Virulence to Yr26 has increased in frequency since its first detection in 2008. The objective of this study was to assess the vulnerability of the wheat cultivars and breeding lines in the Sichuan Basin to Yr26-virulent races. In total, 85 wheat accessions were tested with Yr26-avirulent races CYR32, CYR33, and Su11-4 and two Yr26-virulent races, V26/CM42 and V26/Gui22. DNA markers for Yr26 were used to determine the presence and absence of Yr26 in the wheat accessions. Of the 85 wheat accessions, only 5 were resistant and 19 susceptible to all races tested, and the remaining 61 were resistant to at least one or more races tested in seedling stage. In all, 65 (76.5%) accessions were susceptible to the emerging Yr26-virulent race V26/Gui22. In field tests, susceptible accessions increased from 31.8% in a nursery inoculated with predominant and Yr26-avirulent races to 61.2% in the nursery inoculated with the predominant races mixed with V26/Gui22. Based on the results of the molecular marker and race tests, 33 (38.8%) accessions were determined to have Yr26, showing that the Yr26 virulence is a major threat to wheat production in the Sichuan Basin and potentially in other regions of China.

High-temperature adult-plant resistance to stripe rust in facultative winter wheat

2016 ◽  
Vol 67 (10) ◽  
pp. 1064 ◽  
Author(s):  
Beyhan Akin ◽  
Xian Ming Chen ◽  
Alex Morgunov ◽  
Nusret Zencirci ◽  
Anmin Wan ◽  
...  
Keyword(s):  
Molecular Markers ◽  
High Temperature ◽  
Winter Wheat ◽  
Stripe Rust ◽  
Field Experiments ◽  
Puccinia Striiformis ◽  
Yellow Rust ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Improvement Program

Stripe (yellow) rust, caused by Puccinia striiformis Westend. f. sp. tritici Erikss., is one of the most damaging diseases in wheat and is especially damaging for winter and facultative wheat. The objective of this study was to understand stripe rust resistance in 100 wheat and facultative wheat entries from the International Winter Wheat Improvement Program by conducting experiments in a greenhouse and in four field environments in Washington State, USA, and by genotyping molecular markers linked to Yr genes. Percentages of entries resistant to the rust races at the seedling stage were: PST-17, 44%; PST-37, 32%; PST-43, 45%; PST-45, 49%; PST-116, 18%; PST-100, 17%; and PST-127, 8%. Molecular markers were positive for genes Yr9, Yr17, and Yr18 and negative for Yr5, Yr10, and Yr15. Yr18 was present in 44 entries (44%). By using the highly virulent races PST-127 and PST-100 under controlled conditions, 16 entries were shown to have high-temperature adult-plant (HTAP) resistance and resistant–moderately resistant field reactions at all four field sites. Resistant entries, especially those with HTAP resistance, were also identified in the field experiments.

scholarly journals Quantitative Trait Loci Mapping of Adult Plant and Seedling Resistance to Stripe Rust (Puccinia striiformis Westend.) in a Multiparent Advanced Generation Intercross Wheat Population

2021 ◽  
Vol 12 ◽  
Author(s):  
Sandra Rollar ◽  
Manuel Geyer ◽  
Lorenz Hartl ◽  
Volker Mohler ◽  
Frank Ordon ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Quantitative Trait ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Seedling Resistance ◽  
Wheat Population ◽  
Advanced Generation

Stripe rust caused by the biotrophic fungus Puccinia striiformis Westend. is one of the most important diseases of wheat worldwide, causing high yield and quality losses. Growing resistant cultivars is the most efficient way to control stripe rust, both economically and ecologically. Known resistance genes are already present in numerous cultivars worldwide. However, their effectiveness is limited to certain races within a rust population and the emergence of stripe rust races being virulent against common resistance genes forces the demand for new sources of resistance. Multiparent advanced generation intercross (MAGIC) populations have proven to be a powerful tool to carry out genetic studies on economically important traits. In this study, interval mapping was performed to map quantitative trait loci (QTL) for stripe rust resistance in the Bavarian MAGIC wheat population, comprising 394 F6 : 8 recombinant inbred lines (RILs). Phenotypic evaluation of the RILs was carried out for adult plant resistance in field trials at three locations across three years and for seedling resistance in a growth chamber. In total, 21 QTL for stripe rust resistance corresponding to 13 distinct chromosomal regions were detected, of which two may represent putatively new QTL located on wheat chromosomes 3D and 7D.

Development and characterization of novel wheat-rye 1RS•1BL translocation lines with high resistance to Puccinia striiformis f. sp. tritici

2022 ◽  
Author(s):  
Tianheng Ren ◽  
Qing Jiang ◽  
Zixin Sun ◽  
Zhenglong Ren ◽  
Feiquan Tan ◽  
...  
Keyword(s):  
Stripe Rust ◽  
High Resistance ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Sources Of Resistance ◽  
Chinese Landrace ◽  
New Gene ◽  
Improvement Programs ◽  
Translocation Lines

Wheat-rye 1RS•1BL translocations from Petkus rye have contributed substantially to wheat production worldwide with their great disease resistance and yield traits. However, the resistance genes on the 1RS chromosomes have completely lost their resistance to newly emerged pathogens. Rye could widen the variation of 1RS as a naturally cross-pollinated related species of wheat. In this study, we developed three new 1RS•1BL translocation lines by crossing rye inbred line BL1, selected from Chinese landrace rye Baili, with wheat cultivar Mianyang11. These three new translocation lines exhibited high resistance to the most virulent and frequently occurring stripe rust pathotypes and showed high resistance in the field where stripe rust outbreaks have been most severe in China. One new gene for stripe rust resistance, located on 1RS of the new translocation lines, is tentatively named YrRt1054. YrRt1054 confers resistance to Puccinia striiformis f. sp. tritici pathotypes that are virulent toward Yr9 and YrCn17. This new resistance gene, YrRt1054, is available for wheat improvement programs. The present study indicated that rye cultivars may carry additional untapped variation as potential sources of resistance.

scholarly journals Genome-wide Mapping for Stripe Rust Resistance Loci in Common Wheat Cultivar Qinnong 142

Plant Disease ◽  
2019 ◽  
Vol 103 (3) ◽  
pp. 439-447 ◽  
Author(s):  
Qingdong Zeng ◽  
Jianhui Wu ◽  
Shengjie Liu ◽  
Xianming Chen ◽  
Fengping Yuan ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Wheat Cultivar ◽  
Infection Type ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars

Stripe rust caused by Puccinia striiformis f. sp. tritici threatens worldwide wheat production. Growing resistant cultivars is the best way to control this disease. Chinese wheat cultivar Qinnong 142 (QN142) has a high level of adult-plant resistance to stripe rust. To identify quantitative trait loci (QTLs) related to stripe rust resistance, we developed a recombinant inbred line (RIL) population from a cross between QN142 and susceptible cultivar Avocet S. The parents and 165 F6 RILs were evaluated in terms of their stripe rust infection type and disease severity in replicated field tests with six site-year environments. The parents and RILs were genotyped with single-nucleotide polymorphism (SNP) markers. Four stable QTLs were identified in QN142 and mapped to chromosome arms 1BL, 2AL, 2BL, and 6BS. The 1BL QTL was probably the known resistance gene Yr29, the 2BL QTL was in a resistance gene-rich region, and the 2AL and 6BS QTLs might be new. Kompetitive allele specific polymerase chain reaction markers developed from the SNP markers flanking these QTLs were highly polymorphic in a panel of 150 wheat cultivars and breeding lines. These markers could be used in marker-assisted selection for incorporating the stripe rust resistance QTL into new wheat cultivars.

scholarly journals Point Inoculation Method for Measuring Adult Plant Response of Wheat to Stripe Rust Infection

Plant Disease ◽  
2019 ◽  
Vol 103 (6) ◽  
pp. 1228-1233 ◽  
Author(s):  
Willem H. P. Boshoff ◽  
Renée Prins ◽  
Corneli de Klerk ◽  
Simon G. Krattinger ◽  
Cornelia M. Bender ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Flag Leaf ◽  
Rust Fungus ◽  
Plant Response ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Adaxial Side ◽  
Inoculation Method ◽  
Water Proof

Depending on the pathogenicity of the stripe rust fungus Puccinia striiformis f. sp. tritici, the nature of resistance in the wheat host plant, and the environment, a broad range of disease phenotypes can be expressed. Therefore, the phenotyping of partial adult plant stripe rust resistance requires reliable and repeatable procedures, especially under controlled conditions. In this study, the development of a flag leaf point inoculation method, which resulted in a 100% initial infection rate, is reported. Flag leaf inoculations were achieved by placing 6-mm antibiotic test paper discs, dipped into a urediniospore and water suspension and covered with water-proof plastic tape, on the adaxial side of leaves. Results from independent trials allowed for the statistical comparison of stripe rust lesion expansion rate in wheat entries that differ in resistance. The technique is inexpensive, reliable, and applicable to routine screening for adult plant response type, quantitative comparison of stripe rust progress, environmental influences, and pathogenicity of different isolates.

scholarly journals Barley Stripe Rust Resistance QTL: Development and Validation of SNP Markers for Resistance to Puccinia striiformis f. sp. hordei

2016 ◽  
Vol 106 (11) ◽  
pp. 1344-1351 ◽  
Author(s):  
K. Esvelt Klos ◽  
T. Gordon ◽  
P. Bregitzer ◽  
P. Hayes ◽  
X. M. Chen ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Recombinant Inbred Lines ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Seedling Resistance ◽  
Resistance Qtl ◽  
Barley Stripe Rust

Quantitative trait loci (QTL) for barley stripe rust resistance were mapped in recombinant inbred lines (RIL) from a ‘Lenetah’ × ‘Grannelose Zweizeilige’ (GZ) cross. GZ is known for a major seedling resistance QTL on chromosome 4H but linked markers suitable for marker-assisted selection have not been developed. This study identified the 4H QTL (log of the likelihood [LOD] = 15.94 at 97.19 centimorgans [cM]), and additional QTL on chromosomes 4H and 6H (LOD = 5.39 at 72.7 cM and 4.24 at 34.46 cM, respectively). A QTL on chromosome 7H (LOD = 2.04 at 81.07 cM) was suggested. All resistance alleles were derived from GZ. Evaluations of adult plant response in Corvallis, OR in 2013 and 2015 provided evidence of QTL at the same positions. However, the minor QTL on 4H was not statistically significant in either location/year, while the 7H QTL was significant in both. The single-nucleotide polymorphism markers flanking the resistance QTL were validated in RIL from a ‘95SR316A’ × GZ cross for their ability to predict seedling resistance. In 95SR316A × GZ, 91 to 92% of RIL with GZ alleles at the major 4H QTL and at least one other were resistant to moderate in reaction. In these populations, at least two QTL were required to transfer the barley stripe rust resistance from GZ.

scholarly journals Yr60, a Gene Conferring Moderate Resistance to Stripe Rust in Wheat

Plant Disease ◽  
2015 ◽  
Vol 99 (4) ◽  
pp. 508-511 ◽  
Author(s):  
S. A. Herrera-Foessel ◽  
R. P. Singh ◽  
C. X. Lan ◽  
J. Huerta-Espino ◽  
V. Calvo-Salazar ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Field Trials ◽  
Distal Region ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Plant Responses ◽  
Resistance Locus ◽  
Resistant Parent

Stripe rust, caused by Puccinia striiformis f. sp. tritici W., is a devastating disease of wheat worldwide. A new stripe rust resistance gene with moderate seedling and adult plant resistance was mapped using an F5 recombinant inbred line (RIL) population developed from the cross of the resistant parent ‘Almop’ with the susceptible parent ‘Avocet’. The parents and RILs were phenotyped for seedling stripe rust response variation in a greenhouse and in field trials at Toluca, Mexico for 2 years. Almop showed moderate levels of resistance at both seedling and adult plant stages compared with the highly susceptible response of Avocet. The distribution of homozygous resistant, homozygous susceptible, and segregating RILs conformed to segregation at a single locus. Seedlings and adult plant responses were correlated, indicating that the same gene conferred resistance at both stages. A bulk segregant analysis approach with widely distributed simple sequence repeat (SSR) markers mapped the resistance gene to the distal region of the long arm of chromosome 4A. The SSR marker wmc776 cosegregated with this gene, whereas markers wmc219 and wmc313 were tightly linked and both located at 0.6 centimorgans. The resistance locus was designated Yr60.

Sign in / Sign up

Export Citation Format

Share Document